scholarly journals Microbiota profile in mesophilic biodigestion of sugarcane vinasse in batch reactors

2021 ◽  
Author(s):  
Janaina Iltchenco ◽  
Vanessa Peruzzo ◽  
Flaviane Eva Magrini ◽  
Letícia Marconatto ◽  
Ana Paula Torres ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Metabolic Pathways ◽  
High Throughput Sequencing ◽  
Batch Reactors ◽  
Initial Stage ◽  
Genus Clostridium ◽  
The Family ◽  
Sugarcane Vinasse

Abstract The vinasse is a residue of ethanol production with the potential for methane production, requiring an allochthonous inoculum. Several microorganisms act in the different phases of anaerobic digestion, and the identification of these microbial communities is essential to optimize the process. The characterization of the microbiota involved in the biodigestion of vinasse was observed in the initial stage (IS), at the peak of methane production (MS) and the end of the process (FS) of the best performance assay by high-throughput sequencing. The highest methane production was 0.78 mmolCH4.gVS.h−1 at 243.7 h in the substrate/inoculum ratio of 1.7, with consumption partial of acetic, propionic and isobutyric acids and an 82% reduction of COD. High microbial diversity was found. The genus Clostridium, Acinetobacter, Candidatus Cloacamonas, Bacteroides, Syntrophomonas, Kosmotoga, the family Porphyromonadaceae and the class Bacteroidia were the most abundant in the maximum methane production. Methane production was driven by Methanobacterium and Methanosaeta, suggesting the metabolic pathways using were hydrogenotrophic and acetoclastic.

scholarly journals Effects of sulfonamide antibiotics on digestion performance and microbial community during swine manure anaerobic digestion

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Shaona Wang ◽  
Kang Du ◽  
Rongfang Yuan ◽  
Huilun Chen ◽  
Fei Wang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Anaerobic Digestion ◽  
Dissolved Organic Matter ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
High Throughput Sequencing ◽  
Batch Reactor ◽  
Inhibition Mechanism ◽  
Sulfonamide Antibiotics ◽  
High Concentration

The effects of four types of sulfonamide antibiotics (SAs), including sulfaquinoxaline, sulfamethoxazole, sulfamethoxydiazine and sulfathiazole, on the digestion performance during anaerobic digestion process were studied using a lab-scale anaerobic sequencing batch reactor, and the changes of the community structure in the presence of SAs were investigated with the help of high throughput sequencing. The results indicated that when SAs were added, the hydrolytic acidification process was inhibited, and the accumulation of volatile fatty acids (VFAs) was induced, resulting in the suppression of methane production. However, the inhibition mechanism of different SAs was quite different. The inhibitory effect of high concentration of SAs on the hydrolysis of solid particulate matter into dissolved organic matter followed the order of sulfaquinoxaline > sulfamethoxydiazine > sulfathiazole > sulfamethoxazole. SAs have obvious inhibitory effects on acidification and methanation of dissolved organic matter, especially sulfathiazole. The richness and the community composition of the microorganism including bacteria and archaea in the digestion system were affected by SAs. Under the effect of SAs, the relative abundance of many microorganisms is negatively correlated with methane production, among which Methanobrevibacter, a kind of Archaea, had the greatest influence on methane production.

Characterization of microbial community in cold-chain hairtail fish by high-throughput sequencing technology

2021 ◽  
Author(s):  
Jiali Xing ◽  
Xiaorong Xu ◽  
Xiaohu Luo ◽  
Ruihang Zheng ◽  
Lingyan Mao ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Flora ◽  
Diversity Indices ◽  
Cold Chain ◽  
Class Level ◽  
The Family ◽  
Dominant Bacteria

Abstract: High-throughput sequencing was used to analyze the microbial communities in the muscle samples of hairtail fish to study their diversity and dynamic changes during cold-chain circulation. The results showed that the richness and diversity of the microbial community in hairtail fish had a transient decline in 0–24 h and decreased after the first rise during 24–216 h. The diversity and richness of bacteria in cold-chain hairtail fish reached the maximum at 168 h. The Shannon and Simpson diversity indices of the bacteria were 2.96 and 0.16, respectively, and their ACE and Chao1 richness indices were 254.84 and 155.10, respectively. In addition, the dominant bacteria were Proteobacteria in the phylum level, Gammaproteobacteria in the class level, Pseudomonadales in the order level, Pseudomonadaceae in the family level, and Pseudomonas in the genus level, and their relative abundance were 80.52%, 72.11%, 76.68%, 23.25%, and 53.50%, respectively. In this study, the structure of bacterial flora and the dominant bacteria in cold-chain hairtail fish were analyzed by high-throughput sequencing to provide a basis for exploring how to maintain the freshness of hairtail fish and for predicting the shelf-life of hairtail fish.

scholarly journals Synthetic Effect of EDTA and Ni2+ on Methane Production and Microbial Communities in Anaerobic Digestion Process of Kitchen Wastes

Processes ◽  
2019 ◽  
Vol 7 (9) ◽  
pp. 590
Author(s):  
Tingting Zhong ◽  
Yali Liu ◽  
Xiaorong Kang
Keyword(s):  
Anaerobic Digestion ◽  
Propionic Acid ◽  
Methane Production ◽  
High Throughput Sequencing ◽  
Ethylenediaminetetraacetic Acid ◽  
Speciation Analysis ◽  
Water Soluble ◽  
Sequencing Analysis ◽  
Biogas Yield ◽  
Ni Addition

Batch tests were carried out to study the effect of simultaneous addition of ethylenediaminetetraacetic acid and Ni2+ (EDTA-Ni) on anaerobic digestion (AD) performances of kitchen wastes (KWs). The results indicated that the cumulative biogas yield and methane content were enhanced to 563.82 mL/gVS and 63.7% by adding EDTA-Ni, respectively, which were almost 1.15 and 1.07-fold of that in the R2 with Ni2+ addition alone. At the same time, an obvious decrease of propionic acid was observed after EDTA-Ni addition. The speciation analysis of Ni showed that the percentages of water-soluble and exchangeable Ni were increased to 38.8% and 36.3% due to EDTA-Ni addition, respectively. Also, the high-throughput sequencing analysis revealed that the EDTA-Ni promoted the growth and metabolism of Methanosarcina and Methanobacterium, which might be the major reason for propionic acid degradation and methane production.

scholarly journals Molecular Characterization of the Complete Coding Sequence of Olive Leaf Yellowing-Associated Virus

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1272
Author(s):  
Ana Belén Ruiz-García ◽  
Thierry Candresse ◽  
Celia Canales ◽  
Félix Morán ◽  
Carlos Machado de Oliveira ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Genomic Sequence ◽  
Olive Tree ◽  
Open Reading Frames ◽  
Olive Leaf ◽  
Coding Sequence ◽  
The Family ◽  
Leaf Yellowing ◽  
Yellowing Disease

Genome organization and phylogenetic relationships of olive leaf yellowing-associated virus (OLYaV) with other members of the Closteroviridae family were determined. The complete coding sequence of OLYaV was obtained by high throughput sequencing of total RNA from a 35-year-old olive tree (cv. Zarzaleña) from Brazil, showing olive leaf yellowing disease and deformations in the wood. This represents the first report of OLYaV in this country. A genomic sequence of 16,700 nt containing 11 open reading frames (ORFs) was recovered, representing the complete virus coding capacity. The knowledge of the nucleotide sequence of the genome including the gene that codes the coat protein will facilitate the development of diagnostic tests, which are limited so far to PCR-based methods targeting the HSP70h gene. Interestingly, a thaumatin-like protein (ORF2), previously reported in other unassigned viruses in the Closteroviridae family, persimmon virus B and actidinia virus 1, was identified in the OLYaV genome. Phylogenetic analysis of shared proteins (ORF1a, ORF1b, HSP70h, HSP90h and CP) with all members of the Closteroviridae family provides new insight into the taxonomic position of these three closteroviruses and suggests they could represent a new genus in the family.

scholarly journals Characterization of the Mycovirome of the Phytopathogenic Fungus, Neofusicoccum parvum

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 375
Author(s):  
Armelle Marais ◽  
Chantal Faure ◽  
Gwenaëlle Comont ◽  
Thierry Candresse ◽  
Elodie Stempien ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Phylogenetic Analyses ◽  
Phytopathogenic Fungus ◽  
Sequencing Analysis ◽  
Single Strain ◽  
Neofusicoccum Parvum ◽  
The Family ◽  
Pcr Products ◽  
Botryosphaeria Dieback

Neofusicoccum parvum is a fungal plant-pathogen belonging to the family Botryosphaeriaceae, and is considered one of the most aggressive causal agents of the grapevine trunk disease (GTD) Botryosphaeria dieback. In this study, the mycovirome of a single strain of N. parvum (COLB) was characterized by high throughput sequencing analysis of total RNA and subsequent bioinformatic analyses. Contig annotations, genome completions, and phylogenetic analyses allowed us to describe six novel mycoviruses belonging to four different viral families. The virome is composed of two victoriviruses in the family Totiviridae, one alphaendornavirus in the family Endornaviridae, two mitoviruses in the family Mitoviridae, and one narnavirus belonging to the family Narnaviridae. The presence of the co-infecting viruses was confirmed by sequencing the RT-PCR products generated from total nucleic acids extracted from COLB. This study shows that the mycovirome of a single N. parvum strain is highly diverse and distinct from that previously described in N. parvum strains isolated from grapevines.

scholarly journals Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms

2021 ◽  
Vol 12 ◽  
Author(s):  
Xudong Fan ◽  
Chen Li ◽  
Zunping Zhang ◽  
Fang Ren ◽  
Guojun Hu ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
High Throughput Sequencing ◽  
Rna Virus ◽  
Reading Frame ◽  
A Genome ◽  
The Family ◽  
Pcr Products ◽  
Chlorotic Mottling

A novel negative-sense, single-stranded (ss) RNA virus was identified in a “Shennong Jinhuanghou” (SJ) grapevine showing severe chlorotic mottling symptoms by integrating high-throughput sequencing (HTS) and conventional Sanger sequencing of reverse transcription polymerase chain reaction (RT-PCR) products. The virus was provisionally named as “grapevine emaravirus A” (GEVA). GEVA had a genome comprising five genomic RNA segments, each containing a single open reading frame on the viral complementary strand and two untranslated regions with complementary 13- nt stretches at the 5′ and 3′ terminal ends. RNA1 (7,090 nt), RNA2 (2,097 nt), RNA3 (1,615 nt), and RNA4 (1,640 nt) encoded putative proteins P1–P4 that, based on their conserved motifs, were identified as the RNA-dependent RNA polymerase, glycoprotein, nucleocapsid protein, and movement protein, respectively. However, the functional role of protein P5 encoded by RNA5 (1,308 nt) could not be determined. Phylogenetic trees constructed based on amino acids of P1 to P4, allocated GEVA in clade I, together with other species-related emaraviruses. These data support the proposal that GEVA is a representative member of a novel species in the genus Emaravirus of the family Fimoviridae. Moreover, when GEVA was graft-transmitted to SJ and “Beta” grapevines, all grafted plants showed the same symptoms, similar to those observed in the source of the inoculum. This is the first report to our knowledge of an emaravirus infecting grapevine and its possible association with chlorotic mottling symptoms.

Anaerobic co-digestion of winery wastewater

2007 ◽  
Vol 56 (2) ◽  
pp. 49-54 ◽  
Author(s):  
L. Rodríguez ◽  
J. Villaseñor ◽  
F.J. Fernández ◽  
I.M. Buendía
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Reduction Rate ◽  
Operational Performance ◽  
Waste Activated Sludge ◽  
Batch Reactors ◽  
Methane Generation ◽  
Winery Wastewater ◽  
Methane Production Rate ◽  
Mesophilic Conditions

The operational performance of anaerobic batch reactors treating winery wastewater (WW) combined with waste activated sludge (WAS) in different proportions was investigated under mesophilic conditions. In these experiments it was shown that for anaerobic digestion of WW alone, methane production rate was lower than the rates achieved when WW and WAS were treated together. When WW was mixed with WAS at a concentration of 50% WW resulted in the highest methane production rates. A simplified anaerobic model was used to determine the main kinetic parameters; maximum COD reduction rate (qDA) and maximum methane generation rate (kmax). The maximum values of qDA and kmax were 16.50 kgCOD COD−1 d−1 and 14.34 kgCOD kgCOD−1 d−1, respectively.

Metataxonomic characterization of bacterial and archaeal community involved in hydrogen and methane production from citrus peel waste (Citrus sinensis L. Osbeck) in batch reactors

2021 ◽  
Vol 149 ◽  
pp. 106091
Author(s):  
Franciele Pereira Camargo ◽  
Isabel Kimiko Sakamoto ◽  
Iolanda Cristina Silveira Duarte ◽  
Edson Luiz Silva ◽  
Maria Bernadete Amâncio Varesche
Keyword(s):  
Methane Production ◽  
Citrus Sinensis ◽  
Archaeal Community ◽  
Batch Reactors ◽  
Citrus Peel

scholarly journals Energy Recovery from Anaerobic Digestion of Banana Peels

2019 ◽  
Vol 8 (3) ◽  
pp. 6269-6273
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Biogas Production ◽  
Kinetic Modelling ◽  
Batch Reactor ◽  
Waste Product ◽  
Banana Peel ◽  
Batch Reactors ◽  
Order Kinetic ◽  
First Order

Bananas are tropical fruits mostly eaten in Malaysia. The banana peels are high in organic, and putrescible caused the odour and leachate problem where it has been a dump. In practice, banana peels considered as a waste product that has been combined with municipal solid waste and dumped into the landfills. However, banana peels are bountiful in organic matter and high with moisture content. Thus, it could be a convincing substrate for biogas production through anaerobic digestion so that the major concerns of environmental protection is achieved aside from producing energy in a sustainable way. Therefore, this study was initiated to estimate the ultimate methane yield from the unripe banana peel (UBP) and ripe banana peel (RBP). Besides that, the assessment on the kinetics of the methane production from UBP and RBP is conducted using Modified Gompertz and first-order kinetic modelling. In this study, the anaerobic digestibility of banana peels measured in a batch reactor for 25 days each fed by UBP and RBP. The batch reactors operated at an inoculum to substrate ratio (I/S) of 1.0 and at a mesophilic temperature (37°C). The ultimate methane yields from UBP and RBP digestion were 847.57mLCH4 /gVS and 1405.31mLCH4 /gVS, respectively. The higher bioavailability (in term of COD, and solid) in RBP resulted in the higher methane production rate. Two first-order and modified Gompertz kinetic models were compared for the prediction of organic degradation, and the results indicated that the first-order kinetic model of the RBP fitted the experiment best. It concluded that ripe banana peels are the most preferable feedstock for the anaerobic digestion.

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