Molecular Characterization of Two Polysaccharides from Phellinus vaninii Ljup and their Cytotoxicity to Cancer Cell Lines

2019 ◽  
Vol 18 (9) ◽  
pp. 1356-1363
Author(s):  
Xuewei Jia ◽  
Mingqi Gao ◽  
Mingzhe Li ◽  
Yan Wu ◽  
Ying Zeng ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Molecular Conformation ◽  
Sodium Hydroxide Solution ◽  
Fruit Body ◽  
Water Soluble ◽  
Hot Water ◽  
Active Components ◽  
Molecular Conformations ◽  
Chemical Structures

Background: Phellinus vaninii, a medicinal basidiomycete fungus, is often confused with Phellinus igniarius and Phellinus linteus. Polysaccharides extract from P. igniarius and P. linteus are reported to stimulate humoral immunity and inhibit tumor growth However, available literature reviewed no information on the relationship between bioactivities and structures of polysaccharides from the fruit body of P. vaninii. Methods: Two water-soluble polysaccharides (PV-W, PV-B) were isolated and purified from fruiting bodies of P. vaninii by hot water and sodium hydroxide solution, respectively. The chemical structures of PV-W, PV-B were analyzed by FT-IR, GC-MS analysis and 13C NMR spectra. And, their molecular conformations were analyzed by viscosity method and SEC-MALLS-RI. Finally, their inhibition of cancer cells was investigated using MTT assay. Results: The results illustrated that PV-W was a heteropolysaccharide, mainly composed of mannose, glucose, arabinose and galactose. PV-B was a β-1, 3-D-glucan branched with β-1, 6-D-glucose. The results of viscometry proved that PV-W and PV-B could be molecularly dispersed in water without aggregation. The results of SECMALLS- RI indicated that the two polysaccharides had the similar Mw but different molecular conformation. That is, PV-W existed as a stable globular shape, while PV-B presented a more expanded flexible random coils conformation. MTT assay indicated that PV-B showed higher inhibition effect on HepG2 and HeLa cells than PV-W in vitro. Conclusion: This work provided the important information of active components from P. vaninii and its potential applications in the food and medicine industry.

scholarly journals Apple Pomace Extract as a Sustainable Food Ingredient

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 189 ◽  
Author(s):  
Pedro A. R. Fernandes ◽  
Sónia S. Ferreira ◽  
Rita Bastos ◽  
Isabel Ferreira ◽  
Maria T. Cruz ◽  
...  
Keyword(s):  
Solid Phase ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Apple Pomace ◽  
Water Soluble ◽  
Hot Water ◽  
Array Detector ◽  
Food Ingredient ◽  
Sustainable Solutions

Apple pomace is a by-product of apple processing industries with low value and thus frequent disposal, although with valuable compounds. Acidified hot water extraction has been suggested as a clean, feasible, and easy approach for the recovery of polyphenols. This type of extraction allowed us to obtain 296 g of extract per kg of dry apple pomace, including 3.3 g of polyphenols and 281 g of carbohydrates. Ultrafiltration and solid-phase extraction using C18 cartridges of the hot water extract suggested that, in addition to the apple native polyphenols detected by ultra-high-pressure liquid chromatography coupled to a diode-array detector and mass spectrometry UHPLC-DAD-ESI-MSn, polyphenols could also be present as complexes with carbohydrates. For the water-soluble polyphenols, antioxidant and anti-inflammatory effects were observed by inhibiting chemically generated hydroxyl radicals (OH•) and nitrogen monoxide radicals (NO•) produced in lipopolysaccharide-stimulated macrophages. The water-soluble polyphenols, when incorporated into yogurt formulations, were not affected by fermentation and improved the antioxidant properties of the final product. This in vitro research paves the way for agro-food industries to achieve more diversified and sustainable solutions towards their main by-products.

scholarly journals Production of Ulvan Oligosaccharides with Antioxidant and Angiotensin-Converting Enzyme-Inhibitory Activities by Microbial Enzymatic Hydrolysis

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 160
Author(s):  
Yueh-Hao Ronny Hung ◽  
Guan-Wen Chen ◽  
Chorng-Liang Pan ◽  
Hong-Ting Victor Lin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Antioxidant Activities ◽  
Ace Inhibition ◽  
Aeromonas Salmonicida ◽  
Water Soluble ◽  
Hot Water ◽  
Converting Enzyme ◽  
Green Seaweed ◽  
Ft Ir

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red and brown seaweeds, fewer studies have focused on the biological activity of green seaweed’s oligosaccharides. This study aimed to produce bioactive ulvan oligosaccharides via enzymatic hydrolysis from green seaweed Ulva lactuca. Ulvan, a water-soluble polysaccharide, was obtained by hot water extraction. Two isolated marine bacteria, Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, were used to produce multiple hydrolases, such as ulvanolytic enzymes, amylase, cellulase, and xylanase, to degrade the ulvan extract. An ultrafiltration system was used to separate the enzymatic hydrolysate to acquire the ulvan oligosaccharides (UOS). The characteristics of the ulvan extract and the UOS were determined by yield, reducing sugar, uronic acid, sulfate group, and total phenols. The FT-IR spectrum indicated that the ulvan extract and the UOS presented the bands associated with O-H, C=O, C-O, and S=O stretching. Angiotensin I converting enzyme (ACE) inhibition and antioxidant activities in vitro were evaluated in the ulvan extract and the UOS. These results provide a practical approach to producing bioactive UOS by microbial enzymatic hydrolysis that can benefit the development of seaweed-based products at the industrial scale.

Curry Leaf and its Antioxidant Potential: A Systematic Study to Enhance its Activity in Aqueous Medium

2020 ◽  
Vol 16 (3) ◽  
pp. 323-332
Author(s):  
Deepa Kumari ◽  
Tamanna Mallick ◽  
Abhijit Karmakar ◽  
Samiran Mondal ◽  
Sreeparna Das ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Medium ◽  
Systematic Study ◽  
Water Soluble ◽  
Active Components ◽  
Chemical Methods ◽  
Lady’S Finger ◽  
Encapsulation Process ◽  
Set Up

Background: We have done a systematic study on the antioxidant activity of the methanol and petroleum ether (60-80°C) extracts (MEC and PEC respectively) of Curry leaves (Murraya koenigii Spreng. Family: Rutaceae) using various in-vitro chemical methods. Methods: Both of these two extracts were found to be highly efficient in the formation of Ag and Au nanoparticles. So, we have explored their ability to form the nanoparticles to study their antioxidant activity. In all the assay systems, MEC showed higher activity over PEC in aqueous medium. This may be due to the higher solubility of MEC and its active components, like polyphenols and flavonoids in the aqueous medium. PEC contains lesser amount of these water soluble active components but PEC was rich in carbazole types of alkaloids which are hydrophobic in nature. So, to enhance the antioxidant activity of PEC and its carbazole constituents, like 2-hydroxy carbazole and mahanimbine, we have encapsulated these in the biopolymeric matrix of the mucilage isolated from an edible vegetable, Abelmoschus esculentus L. (commonly known as Lady's finger, family: Malvaceae). Results: It was interesting to note that, PEC and its carbazole compounds showed better antioxidant activity (ferrous ion chelation and ferric reducing antioxidant activity) in aqueous medium after this encapsulation process. Conclusion: The protocols used in the present study were very simple and can be implemented in any lab set-up. In future, this work can be extended to evaluate antioxidant potentials of other plant based materials.

Characterization and Antitumor Activity of a Glucan Structure-Activity Relationship Analysis

2013 ◽  
Vol 726-731 ◽  
pp. 47-49
Author(s):  
Shi Gang Li ◽  
Yong Qi Zhang
Keyword(s):  
Antitumor Activity ◽  
Therapeutic Potential ◽  
Structural Characteristics ◽  
Average Molecular Weight ◽  
Periodate Oxidation ◽  
Water Soluble ◽  
Hot Water ◽  
Human Gastric Cancer ◽  
Relationship Analysis

A water-soluble polysaccharide named as HPS-1 was isolated from the roots of Hedysarum polybotrys Hand.-Mazz by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its antitumor activity. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, GLCMS, methylation analysis, Periodate oxidation and Smith degradation. Based on the data obtained, HPS-1 was found to be an α- (14)-D-glucan, with a single α-D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 9.4×104 Da. MTT assay revealed that HPS-1 significantly inhibited the proliferation of Human hepatocellular carcinoma HEP-G2 cells and human gastric cancer MGC-803 cells in vitro, indicating HPS-1 could have a possible cancer therapeutic potential.

The question of grain content and forage quality in maize: comparisons between isogenic fertile and sterile plants

1975 ◽  
Vol 85 (3) ◽  
pp. 455-463 ◽  
Author(s):  
E. S. Bunting
Keyword(s):  
Dry Matter ◽  
Water Soluble ◽  
Hot Water ◽  
Soluble Carbohydrates ◽  
Maize Breeding ◽  
Forage Maize ◽  
Yield And Quality ◽  
Crop Development ◽  
Grain Formation

SUMMARYResults are presented from four experiments in which comparisons were made between isogenic sterile and fertile plants of maize to study the effects of grain content on yield and quality of shoot dry matter. In two experiments comparisons were made over a range of densities, extending from 5 to 25 plants/m2 in 1972 and 5 to 20 plants/m2 in 1973. The other two experiments, grown in 1974 at a density of 10 plants/m2, were sampled 30, 60 and 90 days after flowering.In 1972 and 1973 yield of shoot dry material was about 10% higher in fertile plants at the lowest density, 5 plants/m2, but grain formation had a negligible effect at higher densities. In 1974, at 10 plants/m2, the yield advantage for fertile plants was 6–7%. These results are in line with other European data, but show a much smaller effect of grain formation on shoot dry matter yield than is normally recorded in USA trials. The discrepancies probably reflect differences in environmental conditions.Absence of grain had little effect on contents of nitrogen, ash and in vitro digestible dry matter, and increased content of pepsin soluble material and hot water soluble carbohydrates. Percentage content of dry matter in the shoot was higher in fertile plants in 1973, and during the later stages of crop development in 1974.The results suggest that the importance attached to high grain content as an essential requirement for yield and quality in forage maize is exaggerated, and that the restriction of maize breeding and testing programmes to assessments of grain production, in the belief that the best grain varieties will also be best for forage, can no longer be justified in northern European countries.

scholarly journals In Vitro Evaluation of Cocoa Pod Husk Pectin as a Carrier for Chronodelivery of Hydrocortisone Intended for Adrenal Insufficiency

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Ofosua Adi-Dako ◽  
Kwabena Ofori-Kwakye ◽  
Mariam El Boakye-Gyasi ◽  
Samuel Oppong Bekoe ◽  
Samuel Okyem
Keyword(s):  
Drug Release ◽  
Adrenal Insufficiency ◽  
Aqueous Medium ◽  
Water Soluble ◽  
Hot Water ◽  
Matrix Tablets ◽  
Lag Phase ◽  
Wet Granulation ◽  
Cocoa Pod Husk

This study evaluated the in vitro potential of cocoa pod husk (CPH) pectin as a carrier for chronodelivery of hydrocortisone intended for adrenal insufficiency. FTIR studies found no drug-CPH pectin interactions, and chemometric analysis showed that pure hydrocortisone bears closer similarity to hydrocortisone in hot water soluble pectin (HWSP) than hydrocortisone in citric acid soluble pectin (CASP). CPH pectin-based hydrocortisone matrix tablets (~300 mg) were prepared by direct compression and wet granulation techniques, and the tablet cores were film-coated with a 15% HPMC formulation for timed release, followed by a 12.5% Eudragit® S100 formulation for acid resistance. In vitro drug release studies of the uncoated and coated matrix tablets in simulated gastrointestinal conditions showed that wet granulation tablets exhibit greater retardation of drug release in aqueous medium than directly compressed tablets. CASP showed greater suppression of drug release in aqueous medium than HWSP. Wet granulation HWSP-based matrix tablets coated to a final coat weight gain of ~25% w/w were optimized for chronodelivery of hydrocortisone in the colon. The optimized tablets exhibited a lag phase of ~6 h followed by accelerated drug release in the colonic region and have potential to control night time cortisol levels in patients with adrenal insufficiency.

scholarly journals Isolation and Bioactivity Analysis of Ethyl Acetate Extract fromAcer tegmentosumUsing In Vitro Assay and On-Line Screening HPLC-ABTS+System

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Kwang Jin Lee ◽  
Na-Young Song ◽  
You Chang Oh ◽  
Won-Kyung Cho ◽  
Jin Yeul Ma
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Hot Water ◽  
Assay Method ◽  
Vitro Assay ◽  
Chemical Structures ◽  
Freeze Dried ◽  
On Line

TheAcer tegmentosum(3 kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (n-BuOH), and water. From the EA extract fraction (1.24 g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. Based on spectroscopic methods such as1H-NMR,13C-NMR, and LC/MS the chemical structures of the compounds were confirmed as feniculin (1), avicularin (2), (+)-catechin (3), (−)-epicatechin (4), and 6′-O-galloyl salidroside (5). Moreover, a rapid on-line screening HPLC-ABTS+system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed. The results indicated that compounds1and2were first isolated from theA. tegmentosum. The anti-inflammatory activities and on-line screening HPLC-ABTS+assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity ofA. tegmentosum.

scholarly journals Assessment of the Quality and Evaluation of the Antioxidant Potential of a Novel Sri Lankan Ayurvedic Polyherbal Formulation

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
H. P. Wakkumbura ◽  
W. M. D. Wickramaarachchi ◽  
L. D. A. M. Arawwawala ◽  
J. A. Liyanage ◽  
R. P. V. J. Rajapakse
Keyword(s):  
Cold Water ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Hot Water ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Phytochemical Screening ◽  
Anti Inflammatory ◽  
Water Extractable

Background. In Sri Lanka, a Polyherbal Ayurvedic Formulation (PHAF), which consists of powders of seven medicinal plants, is being trialed for use as an anti-inflammatory drug. In general, anti-inflammatory drugs have good antioxidant properties. Therefore, in the present study, an attempt was made to assess the quality and evaluate the antioxidant potential of PHAF. Methods. Physicochemical parameters such as ash content, extractable matter, phytochemical screening for secondary metabolites, levels of heavy metals, and microbes were determined according to standard protocols. Antioxidant activity was evaluated using five in vitro assays: total polyphenolic content (TPC), total flavonoid content (TFC), ORAC (oxygen radical absorbance capacity), DPPH (1,1-diphenyl-2-picryl-hydrazyl), and ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt. Results. PHAF contained 5.6 ± 0.2% of moisture, 6.5 ± 0.1% of total ash, 1.4 ± 0.1% of water soluble ash, 0.9 ± 0.0% of acid insoluble ash, 7.7 ± 0.2% of hot water extractable matter, 3.9 ± 0.1% of cold water extractable matter, 10.5 ± 4.1% of hot-ethanol extractable matter, and 8.4 ± 0.2% of cold-ethanol extractable matter. Phytochemical screening revealed the presence of phenolic compounds, tannins, flavonoids, coumarins, and saponins in both aqueous and ethanolic extracts of the drug. TPC, TFC, ORAC, DPPH, and ABTS of aqueous and ethanol extracts of PHAF were 103.65 ± 4.94 and 327.07 ± 9.65 mg gallic acid equivalents/g extract, 76.6 ± 5.83 and 224.6 ± 8.42 mg quercetin equivalents/g of extract, 481.11 ± 17.30 and 1481.44 ± 30.20 mg trolox equivalents/g of extract, 79.50 ± 4.42 and 227.17 ± 6.16 mg trolox equivalents/g of extract, and 198.20 ± 4.55 and 577.08 ± 5.48 mg trolox equivalents/g of extract, respectively. Conclusion. Ethanolic extract of PHAF is better than aqueous extract in terms of antioxidant properties.

scholarly journals Identification of Scopoletin and Chlorogenic Acid as Potential Active Components in Sunflower Calathide Enzymatically Hydrolyzed Extract towards Hyperuricemia

2021 ◽  
Vol 11 (21) ◽  
pp. 10306
Author(s):  
Huining Dai ◽  
Shuai Lv ◽  
Xueqi Fu ◽  
Wannan Li
Keyword(s):  
Chlorogenic Acid ◽  
Glucose Transporter ◽  
Water Soluble ◽  
Active Components ◽  
Expression Levels ◽  
Binding Pockets ◽  
Glucose Transporter 9 ◽  
Agricultural Byproduct ◽  
Soluble Components

It is known that sunflower (Helianthus annuus L.) calathide enzymatically hydrolyzed extract (SCHE) contributes to the regulation of serum uric acid (UA); however, evidence regarding its bioactive components and mechanism are lacking. We identified two water-soluble components (scopoletin and chlorogenic acid) that are abundant in sunflower calathide, especially evaluated for the inhibition of xanthine oxidase (XO) and the expression levels of urate transporters with SCHE. Molecular docking of a chlorogenic acid–XO complex was more stable than that of the Scopoletin–XO, and its binding pockets, which closed the Mo = S center, was similar to xanthine pockets. Moreover, chlorogenic acid exhibited stronger inhibition than that of the scopoletin below 260 μM, despite the IC50 of scopoletin (577.7 μM) being lower than that chlorogenic acid (844.7 μM) on the UA generation assessed by a spectrophotometer in vitro. It revealed that chlorogenic acid and scopoletin were competitive inhibitors of XO. In addition, the SCHE (300 μg/mL) and chlorogenic acid (0.75 mM) obviously inhibited urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) expression levels, while scopoletin significantly upregulated the expression of GLUT9. To summarize, chlorogenic acid served a crucial role in UA regulation consistent with the SCHE and functioned as an important ingredient of SCHE. The strategic analysis of SCHE combined with scopoletin and chlorogenic acid may contribute to the development of food supplemental alternatives on UA metabolism and the reduction of agricultural byproduct waste.

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