scholarly journals Effects of Xylopia aethiopica Pod Extract on Reproductive Hormones in Female Wistar Rats

2021 ◽  
pp. 1-9
Author(s):  
C. G. Okonkwo ◽  
H. A. Ogbunugafor ◽  
A. A. Oladejo
Keyword(s):  
Luteinizing Hormone ◽  
Wistar Rats ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Standard Drug ◽  
Significant Difference ◽  
Female Wistar Rats ◽  
Xylopia Aethiopica

Plants and their metabolites have been used in the treatment of various disease conditions from time immemorial. This study investigated the effect of hydro-ethanolic extract of pods of Xylopia aethiopica on reproductive hormone (follicle stimulating hormone (FSH), luteinizing hormone (LH) and progesterone) in female Wistar rats. Thirty female wistar rats were randomly divided into 5 groups of six animals per group, per cage (n=6). Group A served as the normal control group and was administered distilled water while group B served as positive control and was administered 5 mg of standard drug (Clomid-Clomiphene Citrate). Groups C, D and E served as the treatment group and was administered 50 mg/kg, 100 mg/kg and 200 mg/kg extract of X. aethiopica respectively for seven days. Administration of drugs and extract was done via oral route. The rats were thereafter copulated with their male counterparts for 72 hours and the female rats were observed throughout their gestation and the numbers of litters produced were recorded. The hormonal assay was carried out using Enzyme Linked Immunosorbent Assay. Result showed a significant increase in FSH and Progesterone level (p<0.05) in treated group when compared with the control group while no significant difference (p>0.05) was observed for the luteinizing hormone in the treated groups. The work thus showed that the plant could exert a positive effect on female fertility by enhancing levels of female reproductive hormones.

scholarly journals SUBCHRONIC ORAL TOXICITY TEST FOR 28 DAYS OF SAUROPUS ANDROGYNOUS L. MERR. LEAVES SOUP IN WISTAR FEMALE RATS

2019 ◽  
pp. 31-34
Author(s):  
AGUSLINA KIRTISHANTI ◽  
RIDHO ISLAMIE
Keyword(s):  
Wistar Rats ◽  
Information Source ◽  
Liver Volume ◽  
Weight Ratio ◽  
Female Rats ◽  
Toxicity Tests ◽  
Control Group ◽  
Oral Toxicity ◽  
Significant Difference ◽  
Female Wistar Rats

Objective: The objective of the study was to evaluate the subchronic toxicity of Sauropus androgynus L. Merr. leaves soup. Methods: Subchronic oral toxicity tests were carried out for 28 days in female Wistar rats using conventional methods. Thirty rats were divided into six groups, namely, one control group and three test groups with each extract being given at a dose of 500 mg/kg body weight (BW), 1000 mg/kg BW, and 3000 mg/kg BW, and one group satellite control and satellite test group with doses of 3000 mg/kg BW were carried out for 14 days after 28 days of treatment to see the effects of reversibility. All rat groups were observed for behavior, development of BW, serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), creatinine serum, ratio of liver and lung organs to BW, and histology of liver and lung. Results: The macroscopic observation of rat’s lung and rat weight ratio did not show a significant difference to the control group (p>0.05). In addition, the ratio of liver volume to BW was significantly different between the doses of 1000 mg/kg BW and 3000 mg/kg BW with satellite groups 3000 mg/kg BW (p<0.05). Levels of SGOT and SGPT as well as liver and lung histopathology scores did not show a significant difference to the control group (p>0.05). However, creatinine serum had the highest increase at a dose of 500 mg/kg BW and a dose of 1000 mg/kg BW. Reversibility effects were not seen after 14 days of the past day given Sauropus androgynous soup for 28 days in female Wistar rats. Conclusion: The given of S. androgynous soup for 28 days for female Wistar rats did not show any specific toxicity effect so that its use was relatively safe for the consumption under 30 days. This study is expected to be the information source about the safety profile of S. androgynus leaves soup consumption.

scholarly journals Comparative Modulation of the Reproductive System by Ethanolleaf Extracts of Asystasia Gangetica Andanthocleista Vogelli in Male Wistar Rats

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Simeon I. Egba ◽  
C. O. Okonkwo ◽  
H. C. Omeoga ◽  
I. E. Ekong
Keyword(s):  
Luteinizing Hormone ◽  
Reproductive System ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Reproductive Hormones ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Male Wistar Rats ◽  
Stimulating Hormone

A number of medicinal plants have been found to influence the level of reproductive hormones and thus affect fertility in the overall. This work evaluated the effects of ethanol leaf extracts of A. gangetica and A. vogelli respectively on some reproductive system parameters in male Wistar rats. A total of sixteen (16) male albino rats were grouped into four (four rats in each group): group A served as normal control, group B received Immunace (Vitabiotics) group C and D received 400 mg/kg body weight of A. gangetica and A. vogelli extracts respectively. Extracts were administered orally to rats for 21 days, after which they were sacrificed by cervical dislocations and blood samples drawn by cardiac puncture. The effect of the extracts on testosterone, follicle stimulating hormone, luteinizing hormone and semen analysis of the test rats were determined using enzyme linked immunosorbent assay (ELISA) and standard techniques. Data collected were analyzed using Graph pad prism V6 and p values < 0.05 were adopted as significant. There was no significant (p>0.05) change in testosterone, FSH and luteinizing hormones in the group administered A. gangetica plant extract relative to the control. There was also no visible difference in the testes weight and sperm morphology relative to the control group. In contrast, administration of A. vogelli extract caused significant (p<0.05) decrease in testosterone and follicle stimulating hormone concentrations from: 1.12 ± 0.20 to 0.89 ± 0.05 and 1.41 ± 0.07 to 1.35 ± 0.12 respectively relative to the control group. While significant (p<0.05) decrease in luteinizing hormone (1.71 ± 0.15) was observed relative to the standard drug group (1.76 ± 0.05). A significant (p<0.05) decrease in sperm count and testes weight was also observed in rats treated with A. vogelli extract relative to the normal control.The results suggest that, A. vogelli extract may cause decreased fertility in male albino rats and could be developed further into potent male contraceptives. A. gangetica on the other hand, had no effect on male reproductive hormones.

scholarly journals Red dragon fruit (Hylocereus polyrhizhus) in preventing collagen decrease in menopause induced rats skin

2020 ◽  
Vol 3 (1) ◽  
pp. 5-8
Author(s):  
Eswariy Chandramogan ◽  
I Wayan Sugiritama ◽  
I Gusti Ayu Dewi Ratnayanti ◽  
I Gusti Nyoman Sri Wiryawan ◽  
Ida Ayu Ika Wahyuniari
Keyword(s):  
Wistar Rats ◽  
Ethanol Extract ◽  
Skin Aging ◽  
Female Rats ◽  
Control Group ◽  
Visual Evaluation ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Significant Difference ◽  
Female Wistar Rats

Background: Skin aging in menopause women was due to fewer collagen numbers in cells and antioxidants are necessary for inhibiting the process of skin aging due to oxidative stress. Red dragon fruits which plant in Indonesia was known to have a high concentration of antioxidants. This study aimed to assess the ethanol extract of red dragon fruit skin effects on the number of collagens on the skin of female Wistar rats which has been ovariectomized. Methods: This experimental research was a post-test only control group design that was conducted on 30 female Wistar rats. Groups were divided into control (P0), 5% ethanol extract (P1), and 15% ethanol extract concentrations (P2). The dosage was given orally to the 30 female rats and left for observation for 30 days. The number of collagens was observed and calculated on visual evaluation of the skin biopsy under a light microscope. Results: The result showed a significant difference between treatment groups and control (p<0.05). The number of collagen are higher in P2 (83.03 ±4.84) and P1 (77.26 ±5.06) than P0 (57.73 ±5.04). Conclusion: Ethanol extract of red dragon fruit skin could increase the number of collagen in rat skin.

scholarly journals Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome

2015 ◽  
Vol 6 (2) ◽  
pp. 67-72
Author(s):  
Manizheh Karami ◽  
Fatemeh Lakzaei ◽  
MohammadReza Jalali Nadoushan
Keyword(s):  
Nitric Oxide ◽  
Polycystic Ovary Syndrome ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Ovary Syndrome ◽  
Crown Rump Length ◽  
Female Wistar Rats

ABSTRACT Background and objective Polycystic ovary syndrome (PCOS) can be induced in Wistar rats by over production of nitric oxide (NO). This study evaluated the efficacy of naloxone on the breeding characteristics of rats suffering from nitric oxide induced PCOS. Materials and methods Twenty-four female Wistar rats(200–250 gm) were kept as virgin under standard conditions. They were divided into four groups (n = 6). One group of the animals received L-arginine (50 mg/kg) intraperitoneally (i.p.) for 9 days/once a day. Another group was administered naloxone hydrochloride (0.4 mg/kg, i.p.) prior to injection of L-arginine. The third group was injected solely naloxone. Control group received saline solution (1 ml/kg, i.p.). After the treatments, all female rats were coupled with the intact males. They were then separated by observation of vaginal plaques; it was considered as day 0 of pregnancy. Eventually, they were operated on days 18 to 19 of the gestation to collect the animals’ ovaries. The samples were studied for pathological evidence. The fetal number and weight along with the fetal crown-rump length (CRL) were measured. Results The ovaries obtained from the L-arginine treated group had large cysts with thickened granulosa cell layer in contrast to those of the control or naloxone treated rats (p < 0.0001). The number of fetus though showed a decrease in the L-arginine treated rats (3 ± 1), but the fetal weight or fetal CRL did not change (p > 0.05). Conclusion This study may clearly illustrate the polycystic characteristics in the L-arginine treated group. It may particularly display the breeding efficacy of naloxone in rats with PCOS. How to cite this article Karami M, Lakzaei F, Nadoushan MRJ. Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome. Int J Infertil Fetal Med 2015;6(2):67-72.

scholarly journals Histological and hormonal studies of calabash chalk on ovarian function in adult female wistar rats

2017 ◽  
Vol 34 (03) ◽  
pp. 173-177
Author(s):  
A. Oyewopo ◽  
K. Obasi ◽  
K. Anumudu ◽  
E. Yawson
Keyword(s):  
Body Weight ◽  
Adult Female ◽  
Wistar Rats ◽  
Ovarian Function ◽  
Female Rats ◽  
Control Group ◽  
Treatment Groups ◽  
Female Wistar Rats ◽  
Group A ◽  
Group B

Abstract Introduction: Calabash chalk is a naturally occurring mineral, chiefly composed of fossilized sea shells. It is prepared from clay and mud mixed with other ingredients, including lead, arsenic, sand and wood ash. Clay consumption is correlated with pregnancy, and also to eliminate morning sickness in women. The aim of this study is to evaluate the effects of calabash chalk on the ovarian function in adult female Wistar rats. Methods: Eighteen (18) adult female Wistar rats. Group A served as the control group, group B received 40 mg/kg body weight of Non-salted calabash chalk while group C received 40 mg/kg body weight of Salted calabash chalk for 14 days. On day 15, the animals were sacrificed for histological and biochemical examination. Results: Results showed a significant (P< 0.05) reduction in follicle stimulating hormone (FSH) levels of the treatment groups when compared with the control group and an increase in luteinizing hormone (LH) levels of the treatment groups when compared with the control group. Histological examination of the ovaries showed severe deterioration of the ovarian follicles, necrosis and follicular atresia. Conclusion: Calabash chalk is toxic to ovarian function. These alterations have been shown to be the leading cause ofinfertility in female rats. Hence, proper monitoring, education, and regulation of the product is needed.

The adrenal cortex in female rats after estradiol or calcium treatment

2011 ◽  
Vol 6 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Verica Milošević ◽  
Vladimir Ajdžanović ◽  
Branko Filipović ◽  
Nataša Nestorović ◽  
Milica Manojlović-Stojanoski ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Zona Glomerulosa ◽  
Female Rats ◽  
Control Group ◽  
Zona Fasciculata ◽  
Calcium Treatment ◽  
Inhibitory Effects ◽  
Serum Aldosterone ◽  
Female Wistar Rats ◽  
Cell Volumes

AbstractAdministration of estradiol or calcium, or combined, represents the classical therapeutic approach in the treatment of some menopausal symptoms. We have studied the effects of estradiol dipropionate (EDP) and calcium glucoheptonate (Ca) on morphological and hormonal features of the adrenal gland in 14-month-old female Wistar rats. The animals were treated with EDP (0.625 mg/kg b.w.) or Ca (11.4 mg/kg b.w.) daily for two weeks, with control rats receiving vehicle alone by the same schedule. The cell volumes in the zona glomerulosa (ZG) and zona fasciculata (ZF) were 11.2% and 5.5% greater (P<0.05) and in the zona reticularis (ZR) 13.0% smaller (P<0.05) in the EDP group than in the control group. In the Ca group, cell volume in the ZG was increased by 5.6% (P<0.05), while cell volumes in the ZF and ZR were decreased by 26.0% and 14.7%, respectively (P<0.05), in comparison with control values. Serum aldosterone and corticosterone concentrations were higher in the EDP-treated (by 27.8% and 19.8%, respectively) and Ca-treated (by 80.0% and 24.1%, respectively) groups in comparison with the control group (P<0.05). These data suggest that EDP and Ca treatments have stimulatory effects on the ZG and ZF, but inhibitory effects on the ZR in middle-aged female rats.

scholarly journals Perbedaan Penurunan Suhu Tubuh Tikus Wistar Jantan Dan Betina Yang Mati Akibat Diinduksi Metanol

2021 ◽  
Vol 4 (2) ◽  
pp. 51
Author(s):  
Abdul Hakim Nitiprodjo ◽  
Titik Kusumawinakhyu
Keyword(s):  
Body Temperature ◽  
Wistar Rats ◽  
Wistar Rat ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Male And Female ◽  
Significant Difference ◽  
Post Test ◽  
Female Wistar Rats

Someone who dies will experience a decrease in body temperature from body temperature at the beginning of death, both within normal and abnormal limits to room temperature. Decrease in body temperature according to sex may differ in duration. Coupled with exposure to methanol, it is also possible to influence a decrease in body temperature of the corpse. This study aimed to analyze the differences in body temperature reduction in male and female Wistar rats  induced with methanol.This research is an experimental study with a pre and post test control group design approach. The design of this study was to observe the body temperature of male and female Wistar rats while still alive and after death and the duration of decrease in body temperature of male and female Wistar mice after being induced with methanol. The population studied was male and female Wistar rat. Based on the results from analysis test, it can be concluded that there is no significant difference between the decrease in body temperature of male and female Wistar rats that died induced by methanol, but there is a significant difference between the decrease in body temperature of male and female Wistar rats who died induced by methanol and without methanol.

scholarly journals Lycopene: Hepatoprotective and Antioxidant Effects toward Bisphenol A-Induced Toxicity in Female Wistar Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Haidy G. Abdel-Rahman ◽  
Heba M. A. Abdelrazek ◽  
Dalia W. Zeidan ◽  
Rasha M. Mohamed ◽  
Aaser M. Abdelazim
Keyword(s):  
Bisphenol A ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Female Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Cytotoxic Effects ◽  
Female Wistar Rats ◽  
Low Levels ◽  
Group 10

Bisphenol A (BPA)—an endocrine disruptor xenoestrogen—is widely spread in the environment. Lycopene (LYC) is an antioxidant phytochemical carotenoid. The hereby study was designed to verify the deleterious effect of BPA on cyclic female rats’ hepatic tissue as well as evaluation of the effect of LYC toward BPA hepatic perturbation. Twenty-eight female Wistar rats were allocated equally into four groups: control group, LYC group (10 mg/kg B.wt), BPA group (10 mg/kg B.wt), and BPA + LYC group (the same doses as former groups). The treatments were given daily via gavage to the rats for 30 days. The rats in BPA displayed high activities of serum liver enzymes with low levels of total proteins (TP) and albumin. Moreover, BPA induced hepatic oxidative stress via depletion of antioxidant enzymes concomitant with augmentation of lipid peroxidation, increased comet tail DNA %, and overexpression of caspase-3. Meanwhile, LYC administration reduced the cytotoxic effects of BPA on hepatic tissue, through improving the liver function biomarkers and oxidant-antioxidant state as well as DNA damage around the control values. These findings were confirmed by hepatic histopathological examination. Finally, LYC credited to have a noticeable protective effect versus BPA provoked oxidative injury and apoptosis of the liver tissue.

scholarly journals Efek konsumsi kopi arabika berlebih selama masa laktasi terhadap densitas tulang mandibula anak tikus WistarThe effect of excessive Arabica coffee consumption during lactation on the mandibular bone density of Wistar rats offspring

2020 ◽  
Vol 32 (3) ◽  
pp. 170
Author(s):  
Shabrina Widya Ardiningrum ◽  
Amandia Dewi Permana Shita ◽  
Dwi Kartika Apriyono
Keyword(s):  
Bone Density ◽  
Wistar Rats ◽  
Coffee Consumption ◽  
Control Group ◽  
Lactation Period ◽  
Mandibular Bone ◽  
Arabica Coffee ◽  
Significant Difference ◽  
Female Wistar Rats ◽  
One Way Anova

Pendahuluan: Laktasi merupakan periode penting dalam pertumbuhan dan perkembangan bayi. Salah satu faktor risiko yang menyebabkan penurunan densitas tulang mandibula adalah konsumsi kopi berlebih selama masa laktasi. Penelitian ini bertujuan untuk menganalisis pengaruh konsumsi kopi Arabika selama masa laktasi terhadap densitas tulang mandibula anak tikus Wistar. Metode: Penelitian eksperimental laboratoris dilakukan terhadap 12 ekor tikus Wistar betina yang sedang menyusui. Penghitungan besar sampel berdasarkan jumlah kelompok penelitian (tiga kelompok), yaitu dengan rumus Arifin, dan didapatkan hasil empat ekor tikus untuk setiap kelompok penelitian, sehingga total jumlah sampel adalah 12 ekor tikus. Tiga kelompok penelitian terdiri dari kelompok kontrol (K) yang diberi akuades, kelompok perlakuan yang diberi kopi dosis normal (P1), dan kelompok perlakuan yang diberi kopi dosis berlebih (P2). Akuades dan kopi diberikan selama 21 hari sejak tikus mulai menyusui anaknya. Masing-masing induk tikus diambil satu anaknya untuk di-euthanasia pada hari ke-22. Kemudian dilakukan pengambilan sampel tulang mandibula dan dilakukan pengambilan rontgen foto periapikal. Selanjutnya dilakukan pengukuran densitas tulang mandibula menggunakan densitometer. Data dianalisis menggunakan uji one-way ANOVA dan LSD. Hasil: Hasil dari uji one-way ANOVA dari densitas tulang mandibula anak tikus menunjukkan bahwa terdapat perbedaan signifikan (p=0,0001) diantara seluruh kelompok penelitian. Hasil uji LSD menunjukkan perbedaan yang signifikan (p=0,0001) antara kelompok kontrol dengan kelompok yang diberi kopi dosis normal maupun dengan kelompok kopi dosis berlebih, demikian juga antara kelompok yang diberi dosis normal dengan dosis berlebih. Simpulan: Konsumsi kopi Arabika dalam dosis berlebih selama masa laktasi pada tikus Wistar dapat menurunkan densitas tulang mandibula anak tikus Wistar. Kata kunci: Laktasi, kopi arabika, densitas tulang mandibula. ABSTRACTIntroduction: Lactation is an important period in the growth and development. One of the risk factors that cause a decrease in mandibular bone density is excessive coffee consumption during lactation. This study was aimed to analyse the effect of coffee consumption, especially Arabica coffee, during lactation period on the rats’ mandibular bone density. Methods: This study was an experimental laboratory study of 12 lactating female Wistar rats. The sample size calculation was based on the number of research groups (three groups), the Arifin formula, and the results were four rats for each research group, so the total number of samples was 12 rats. The three research groups consisted of control group (K) which was given aquadest, treatment group that was given a normal dose of coffee (P1), and a treatment group that was given an excessive dose of coffee (P2). Aquadest and coffee were given for 21 days since the initial lactation period. One offspring of each female rat was taken to be euthanised on the 22nd day. Afterwards, the mandibular bone samples were taken from the young rats, and then the periapical x-rays was performed. Furthermore, the mandibular bone density measurement was carried out using a densitometer. Data was analysed using the one-way ANOVA and LSD tests. Results: The one-way ANOVA test of the mandibular bone density of the rats’ offspring showed a significant difference (p = 0.0001) between all study groups. The LSD test results showed a significant difference (p = 0.0001) between the control group and the group was given the normal dose of coffee and the group with an excessive dose of coffee and between the group that was given the normal and the excessive dose. Conclusion: Consumption of Arabica coffee in excessive dose during lactation of female Wistar rats can reduce the mandibular bone density of the offspring.Keywords: Lactation, arabica coffee, mandibular bone density.

scholarly journals INVESTIGATION OF THE EFFECTS OF ALLOXAN-INDUCED DIABETES ON REPRODUCTIVE HORMONES (FOLLICLE STIMULATING, LUTEINIZING AND PROLACTIN), LIPID PROFILE AND SERUM ELECTROLYTES IN MALE AND FEMALE WISTAR RATS

2020 ◽  
Vol 7 ◽  
pp. 40
Author(s):  
Bukonla Oluyemisi Adegbesan ◽  
Olugbenga Owolabi Ogunlabi ◽  
Temtope Abigail Fasanya ◽  
Ayodeji Adebayo Adelegan ◽  
Emmanuel Oladipupo Ajani
Keyword(s):  
Lipid Profile ◽  
Wistar Rats ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Type1 Diabetes ◽  
Female Wistar Rats ◽  
The Impact ◽  
Female Subjects

Human patients with Type1 diabetes and inadequate metabolic control have been found to exhibit a high prevalence of infertility but the exact mechanism by which this happens has not been fully elucidated. Alterations in the levels of lipids and electrolytes are considered to be good indications of some certain diseases including diabetes. This study aims at elucidating the impact of diabetes on the reproductive functions of male and female subjects as well as the responses of lipid profile which could be a determining factor affecting diabetic condition, to Alloxan-induced diabetes using male and female Wistar rats. To explore this, various hormonal assays assessing reproductive hormones such as Follicle stimulating hormone (FSH); Luteinizing hormone (LH); Prolactin hormone (PRL); lipid profile analyses and electrolytes measurements were employed. Our results showed that diabetes induce significant reduction in the levels of FSH and LH in both male and female rats (p<0.0001); significant reduction in the levels of LH in male (p<0.01) and female (p<0.0001); significant increase in the levels of prolactin (PRL) in male (p<0.05) and female (p<0.0001) ; significant increases in cholesterol levels in both male and female rats (p<0.01), an indication that diabetes is accompanied in most cases by hypercholesterolemia and triglycerides levels in both male (p<0.01) and female (p<0.0005) rats when compared to the control rats. The results obtained in this study shed more light on the mechanism by which fertility in male and female subjects is affected by diabetes and suggest possible mechanisms by which infertility could result from diabetes.

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