scholarly journals A POTENT CYTOTOXICITY AND ANTIMICROBIAL ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESIZED BY LEAF OF IPOMOEA PES-CAPRAE (L.) R. BR.

2019 ◽  
pp. 111-117
Author(s):  
RAMESH A ◽  
SUNDARRAJ P ◽  
BALAMANI J
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Assay Method ◽  
Oxide Nanoparticles ◽  
Visible Spectroscopy ◽  
Zno Nps ◽  
Cytotoxicity Activity ◽  
Uv Visible

Objective: The present study was conducted to investigate the cytotoxicity and antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) synthesized as eco-friendly technique from the leaf extract of Ipomoea pes-caprae (L.) R. Br. against human lung adenocarcinoma (A549), brain tumor (U87) cells, and human pathogens Salmonella typhi, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, and Bacillus subtilis. Materials and Methods: The work was carried out with varying precursor (plant extract) volume to optimize the synthesis of ZnO NPs and it was confirmed by ultraviolet (UV)-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, scanning electron microscopy, and atomic force microscope (AFM) characterization techniques and evaluate its cytotoxicity activity by 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl--tetrazolium bromide assay method, antimicrobial activity by disk diffusion method. Results: A peak at 320 nm with maximum intensity was observed at temperature of 80°C with pH of 8.0 in UV-visible spectroscopy confirmed the formation of ZnO NPs and we calculate the size of ZnO NPs from XRD data found as 15.8 nm. The FTIR analysis evaluated that the presence of different functional groups is carboxyl, amine, and phenolic compounds of leaves extract which are involved in the reduction of zinc ions and acts as capping the ZnO NPs. AFM microgram confirms that ZnO NPs were in nanorange and spherical in nature. The cytotoxicity activity of A549 and U87 cell lines treated with various concentrations of ZnO NPs showed a dose-dependent increase in cell inhibition and the half maximal inhibitory concentration value was calculated to be 7.8 μg/ml. The antibacterial activity of selected pathogens shows higher zone of inhibition. Conclusion: The present study reveals that synthesized ZnO NPs capping with various bioactive compounds present in the leaf of I. pes-caprae show promising activity of cancer cell lines and antimicrobial agents; hence, further detailed study may lead to develop at a novel phytomedicine for the anticancer and antimicrobial drugs.

scholarly journals Plant Mediated Green Synthesis of Zinc Oxide Nanoparticles for Burn Wound Therapy

2020 ◽  
Author(s):  
Mohd Riyaz Beg ◽  
Shital Ghodinde ◽  
Vidhi Gupta
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Zinc Oxide ◽  
Green Synthesis ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Burn Wounds ◽  
Burn Wound Healing

In this changing world, we all are surrounded by the surmountable risk of getting injured. Amongst various risk factors, major burns are the most distressing and catastrophic. Burn wounds are not easy to heal via natural healing process and ultimately ended up with scar formation. If the degree of burn is high then the loss of tissue and its function is very common. To fasten-up the natural burn wound healing; zinc, an essential trace element is found to be very much effective. But due to its’ particle size limitation, less contact with wounded cells and tissues, and high inherent toxicity restrict its use. Needlessly, zinc is an element with dual action i.e. both antimicrobial and wound healing it is a prime choice to apply its aptitude in burn wound healing. To overcome the documented limitations zinc has converted to nanoparticle form. Zinc oxide nanoparticles, in particular, have attained ample of interest due to their unique properties and potential antimicrobial activity along with wound healing activity which makes it promising for the healing of topical burn wounds. Plant mediated green synthesis of nano-metal oxide particles is gaining a lot of significance due to its simplicity, eco-friendliness and extensive antimicrobial activity and recommended as an appealing substitute to not only physical methods but also chemical methods avoiding the use of the high rate of toxic chemicals and extreme surroundings. This study includes ZnO NPs role in burn wound healing with Phyto-mediated synthesis methods to provide evidence of their potential applications. Additionally, it provides an overview of traditional methods used for the synthesis of ZnO nanoparticles and characterization techniques to obtain information concerning the size, shape and optical properties along with toxicity and safety concern of ZnO NPs and its biomedical applications.

scholarly journals Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4896
Author(s):  
Siva Sankar Sana ◽  
Divya Vishambhar Kumbhakar ◽  
Akbar Pasha ◽  
Smita C. Pawar ◽  
Andrews Nirmala Grace ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Migration ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Positive Control ◽  
Ftir Spectra ◽  
Total Phenolic ◽  
Oxide Nanoparticles ◽  
Zno Nps

In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

scholarly journals Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles

2021 ◽  
Vol 22 (17) ◽  
pp. 9529
Author(s):  
Anna Król-Górniak ◽  
Katarzyna Rafińska ◽  
Fernanda Monedeiro ◽  
Paweł Pomastowski ◽  
Bogusław Buszewski
Keyword(s):  
Reactive Oxygen Species ◽  
Methylene Blue ◽  
Zinc Oxide ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Uv Light ◽  
Reactive Oxygen ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Zno Nps

In this paper, a study of the cytotoxicity of bare and functionalized zinc oxide nanoparticles (ZnO NPs) is presented. The functionalized ZnO NPs were obtained by various types of biological methods including microbiological (intra- and extracellular with Lactobacillus paracasei strain), phytochemical (Medicago sativa plant extract) and biochemical (ovalbumin from egg white protein) synthesis. As a control, the bare ZnO NPs gained by chemical synthesis (commercially available) were tested. The cytotoxicity was measured through the use of (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye as well as lactate dehydrogenase (LDH) assays against murine fibroblast L929 and Caco-2 cell lines. As a complementary method, scanning electron microscopy (SEM) was performed to assess the morphology of the tested cells after treatment with ZnO NPs. The microscopic data confirmed the occurrence of apoptotic blebbing and loss of membrane permeability after the administration of all ZnO NPs. The reactive oxygen species (ROS) concentration during the cell lines’ exposure to ZnO NPs was measured fluorometrically. Additionally, the photocatalytic degradation of methylene blue (MB) dye in the different light conditions, as well as the antioxidant activity of bare and functionalized ZnO NPs, is also reported. The addition of all types of tested ZnO NPs to methylene blue resulted in enhanced rates of photo-degradation in the presence of both types of irradiation, but the application of UV light resulted in higher photocatalytic activity of ZnO NPs. Furthermore, bare (chemically synthetized) NPs have been recognized as the strongest photocatalysts. In the context of the obtained results, a mechanism underlying the toxicity of bio-ZnO NPs, including (a) the generation of reactive oxygen species and (b) the induction of apoptosis, is proposed.

scholarly journals The toxic effect of zinc oxide nanoparticles on the terrestrial slug Lehmannia nyctelia (Gastropoda-Limacidae)

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Wafaa A. Mohammad ◽  
Safaa M. Ali ◽  
Nasser Farhan ◽  
Shimaa Mohamed Said
Keyword(s):  
Zinc Oxide ◽  
Toxic Effect ◽  
Zinc Oxide Nanoparticles ◽  
Plant Protection ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
Zno Nps ◽  
X Ray ◽  
Histological Alterations

Abstract Background Chemicals have deleterious effect on the environment. The wide use of nanomaterials as products for plant protection, fertilizers, and also in water purification leads to the release of these materials to the environment. Terrestrial gastropods including snails and slugs have the ability to accumulate heavy metals in their bodies. The present study evaluates the toxic effect of zinc oxide nanoparticles on the terrestrial slug Lehmannia nyctelia. Zinc oxide nanoparticles (ZnO NPs) were prepared by thermal decomposition method. ZnO NPs are characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and ultraviolet-visible spectroscopy (UV). Slugs were treated with three different concentrations of ZnO NPs. Results A total of three animals died by the end of the experiment. Many histological alterations were detected after exposure to different concentration of ZnO NPs. Conclusions The obtained histological alterations prove the toxic effect of ZnO NPs on the animal under study.

Addition of Zinc Oxide Nanoparticles in Biodegradable Polymer and Evaluation of its Antimicrobial Activity

2018 ◽  
Vol 930 ◽  
pp. 230-235 ◽  
Author(s):  
Ana Paula Capelezzo ◽  
Laura Cassol Mohr ◽  
Janayne Sander Godoy ◽  
Alessandra Sgnaulin Bellei ◽  
Luciano Luiz Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Biodegradable Polymer ◽  
Zinc Oxide Nanoparticles ◽  
Antimicrobial Agents ◽  
Food Contamination ◽  
Good Alternative ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Good Antibacterial Activity

The food contamination for pathogenic microorganisms is a serious problem for the food security. Many actions can be taken in order to reduce this pathogenic multiplication, highlighting the use of active packaging for the food conservation, which have antimicrobial agents dispersed in its surface. The metal oxides and its nanoparticles have been shown effective additives for this purpose. With the growing concern with the environmental and the use of less aggressive materials, biodegradable polymers emerge like a good alternative. This work has the goal to investigate the effect on antimicrobial activity against Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) bacteria when added zinc oxide nanoparticles (ZnO NPs) in concentration of 1% (wt/wt) in the biodegrable polymer Ecoflex®. The system was homogenized and proccessed in industrial extruder single screw and the polymer’s antimicrobial activity was evaluated by the agar diffusion tests and counting surviving microorganisms with the time. The results showed that the biodegradable polymer Ecoflex®with ZnO NPs exhibit good antibacterial activity.

Green Synthesis of Zinc Oxide Nanoparticles (ZnO NPs) using Aqueous Extract of Tagetes erecta flower and Evaluation of its Antioxidant, Antimicrobial, and Cytotoxic activities on HeLa cell line.

2020 ◽  
Vol 09 ◽  
Author(s):  
Akila Ilangovan ◽  
Akshaya Venkatramanan ◽  
Pakutharivu Thangarajan ◽  
Anitha Saravanan ◽  
Sowndarya Rajendran ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Cell Line ◽  
Cancer Cell ◽  
Crude Extract ◽  
Zinc Oxide Nanoparticles ◽  
Cancer Cell Line ◽  
Tagetes Erecta ◽  
Oxide Nanoparticles ◽  
Zno Nps

Background: The Zinc oxide Nanoparticles (ZnO NPs) were synthesized successfully by using Tagetes erecta flower aqueous extract and evaluated for their antioxidant potential, antimicrobial and cytotoxic potential. Methods: Phytochemical screening of aqueous crude extract and synthesized ZnO NPs of Tagetes erecta flower revealed the presence of alkaloids, flavonoids, carbohydrates, amino acids, tannins, and proteins, etc. The characterization was done by various spectral analyses. Invitro antioxidant activities of synthesized ZnO NPs were found to possess concentrationdependent free radical scavenging activity was carried on different free radicals i.e. DPPH and ABTS. Antimicrobial activity of synthesized ZnO NPs was done by agar well diffusion method and compared with control ampicillin, while cytotoxic effects were determined by MTT assay against human cervical cancer cell line. Results: UV-Visible spectra were conducted to confirm the synthesis of ZnO NPs and peak obtained at 364.15nm. X-ray analysis confirmed the crystalline nature of the nanoparticles and the average size of the nanoparticles was 30-50nm and was spherical shape analyzed by SEM. The synthesized ZnO NPs showed antimicrobial activity against all tested microorganisms and maximum inhibition zone was found against E.coli in gram-negative and S.aureus in gram-positive bacteria. Synthesized ZnO NPs were showed 50% cell viability at 26.53µg/ml against the HeLa cancer cell line. Conclusion: The conclusion of this study suggests both the aqueous crude extract of Tagetes erecta flower and synthesized ZnO NPs showed an excellent alternative source of antimicrobial agent also an attractive selective cytotoxic activity against HeLa tested cancer lines, offering satisfying ‘safe and cheaper’ alternatives to conventional therapy protocols.

scholarly journals Antimicrobial activity of silymarin mediated zinc oxide and hydroxy apatite nanoparticles against oral pathogens

2020 ◽  
Vol 16 (11) ◽  
pp. 863-868
Author(s):  
S Aravind Kumar ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Candida Albicans ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Pseudomonas Sp ◽  
Hydroxy Apatite ◽  
Oral Pathogens ◽  
Uv Visible

The nanoparticles such as hydroxyapatite, zinc oxide, titanium dioxide and zirconium nanoparticles have application in dentistry. Therefore, it is of interest to document the antimicrobial activity of silymarin mediated zinc oxide and hydroxy apatite nanoparticles against oral pathogens. Hence, we synthesized hydroxyapatie and zinc oxide nanoparticles with silymarin and characterized by UV-visible spectrophotometer. Data shows that silymarin mediated HAP and ZnO nanoparticles have antimicrobial activity against oral pathogens such as Pseudomonas sp, Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Candida albicans.

scholarly journals PARTHENIUM MEDIATED SYNTHESIS OF ZINC OXIDE NANOPARTICLES AND ITS CHARACTERIZATION

2019 ◽  
Vol 11 (1) ◽  
pp. 113
Author(s):  
Siva Kumar Ramamurthy ◽  
Chenchugari Sridhar
Keyword(s):  
High Pressure ◽  
Zinc Oxide ◽  
Plant Extract ◽  
Zinc Oxide Nanoparticles ◽  
Reducing Agent ◽  
Oxide Nanoparticles ◽  
Visible Spectroscopy ◽  
External Energy ◽  
High Pressure And Temperature ◽  
Uv Visible

Objective: To biosynthesize zinc oxide nanoparticles by using parthenium hysterophorous plant extract as a reducing agent and its characterization by spectroscopic techniques.Methods: A novel method was developed to prepare zinc oxide nanoparticles by using zinc nitrate as a precursor and biosynthesis of zinc oxide nanoparticles was mediated by parthenium hysterophorous plant extract without the aid of external energy (high pressure and temperature). This new method involves simple techniques such as centrifugation, filtration, and stirring. Zinc oxide nanoparticles formation was confirmed by analytical techniques such as UV-Visible spectroscopy, powder X-ray diffraction (XRD), Raman spectroscopy and by scanning electron microscopy (SEM) analysis.Results: Zinc oxide nanoparticles were synthesized by using parthenium hysterophorous plant extract as a reducing agent. The XRD measurement showed that zinc oxide nanoparticles possess a typical hexagonal structure and the crystallite size of the synthesized zinc oxide nanoparticles was found to be 32 nm calculated by scherrer’s formula. The SEM images show agglomeration of zinc oxide nanoparticles that are spherical clusters. The maximum absorbance (380 nm) of UV-Visible spectroscopy further confirmed synthesized nanoparticles are zinc oxide. The Raman spectra show both E2 mode and E1 mode, which indicates that the prepared zinc oxide nanoparticles possess crystalline nature with hexagonal wurtzite structure.Conclusion: A method was established to prepare zinc oxide nanoparticles with parthenium hysterophorous plant extract which is a novel approach without the aid of external energy (high pressure and temperature), and formation of zinc oxide nanoparticles was confirmed by spectroscopic techniques. This method can be used in pharmaceutical industry for the synthesis of an antimicrobial agent.

Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa
Keyword(s):  
Zinc Oxide ◽  
Camellia Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Enzymatic Antioxidants ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

2020 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Faizan ◽  
Fangyuan Yu ◽  
Chen Chen ◽  
Ahmad Faraz ◽  
Shamsul Hayat
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Abiotic Stresses ◽  
Zinc Oxide Nanoparticles ◽  
Growth And Yield ◽  
Oxide Nanoparticles ◽  
Main Concern ◽  
Negative Effects ◽  
Zno Nps ◽  
Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

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