Physico-chemical and phytochemical evaluation of Aloe acutissima leaves

2016 ◽  
Vol 5 (06) ◽  
pp. 4633
Author(s):  
Abdul Nasar S.* ◽  
Narasegowda P. N.

Aloe acutissima is a fast growing shrub belonging to the family Xanthorrhoeaceae. This plant is a point of concern as it has profound importance in the field of pharmacology. The secondary metabolites obtained from this plant viz., alkaloids, flavonoids, steroids, quinines, glycosides, tannins, saponins, terpenoids, phenols, proteins, oils and free fatty acids serves as a medicinal tool to mankind. The study comprises of physico-chemical and phytochemical evaluation of leaves of Aloe acutissima by using standard methods. Physico-chemical parameters such as percentage of loss on drying (LOD), ash values, extractive values were determined. Phytochemical evaluation was carried out to detect the presence of alkaloids, carbohydrates, tannins, saponins, flavonoids, quinines, glycosides, terpenoids, phenols, couramins, acids, proteins, oils and free fatty acids in different extracts of Aloe acutissima leaf powder. Estimation of Phenols, Tannin, flavonoids, Alkaloids, steroids and ascorbic acid content of Aloe acutissima leaves were also carried out. Thus the present study revealing the physico-chemical data and phytochemical analysis of leaves of Aloe acutissima is useful for further studies of pharmacological parameters which is helpful for the future at large. 

2021 ◽  
Vol 13 (1) ◽  
pp. 96-104
Author(s):  
Jelili Babatunde Hussein ◽  
Joseph Idowu Olaniyi ◽  
Esther Anjikwi Msheliza ◽  
Seember Bernadette Kave

The partial substitution of margarine with mature avocado pear pulp in the production of cookies was investigated. Five cookie samples were produced with avocado pear pulp and margarine blends in the ratios 80:20%, 70:30%, 60:40%, 50:50%, and 100% margarine serve as the control, labeled as B, C, D and E and A, respectively while the other ingredients used remain constant. The physico-chemical (proximate compositions, free fatty acids (FFA), iodine value, and peroxide value) and sensory qualities of the cookies were evaluated using standard methods. The results show ranged in moisture (11.13 to 14.60%), crude protein (6.93 to 7.83%), crude fat (16.00 to 18.03%), ash (1.40 to 2.09%), crude fiber (0.29 to 0.62%), carbohydrate (59.70 to 62.79%), FFA (0.35 to 1.01 mg KOH/g), iodine value (75.63 to 81.17 g I2/100 g) and peroxide value (2.96 to 5.27 meq/kg). The partial substitution of margarine with avocado pear pulp produced nutritious cookies with desirable organoleptic qualities. Also, the results demonstrated that cookies had acceptability up to a 30% level of substitution with avocado pear pulp. The findings indicated the feasibility of avocado pear pulp in fat-reduced cookies preparation, this will reduce the pressure in using only margarine in cookies making and diversify the use of avocado pear.


Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7383
Author(s):  
Tingting Dong ◽  
Yueqi Sha ◽  
Hairong Liu ◽  
Liwei Sun

Rhodiolacrenulata (Hook.f. & Thomson) H.Ohba is an alpine medicinal plant that can survive in extreme high altitude environments. However, its changes to extreme high altitude are not yet clear. In this study, the response of Rhodiola crenulata to differences in altitude gradients was investigated through chemical, ICP-MS and metabolomic methods. A targeted study of Rhodiola crenulata growing at three vertical altitudes revealed that the contents of seven elements Ca, Sr, B, Mn, Ni, Cu, and Cd, the phenolic components, the ascorbic acid, the ascorbic acid/dehydroascorbate ratio, and the antioxidant capacity were positively correlated with altitude, while the opposite was true for total ascorbic acid content. Furthermore, 1165 metabolites were identified: flavonoids (200), gallic acids (30), phenylpropanoids (237), amino acids (100), free fatty acids and glycerides (56), nucleotides (60), as well as other metabolites (482). The differential metabolite and biomarker analyses suggested that, with an increasing altitude: (1) the shikimic acid-phenylalanine-phenylpropanoids-flavonoids pathway was enhanced, with phenylpropanoids upregulating biomarkers much more than flavonoids; phenylpropanes and phenylmethanes upregulated, and phenylethanes downregulated; the upregulation of quercetin was especially significant in flavonoids; upregulation of condensed tannins and downregulation of hydrolyzed tannins; upregulation of shikimic acids and amino acids including phenylalanine. (2) significant upregulation of free fatty acids and downregulation of glycerides; and (3) upregulation of adenosine phosphates. Our findings provide new insights on the responses of Rhodiola crenulata to extreme high altitude adversity.


2021 ◽  
Vol 6 (2) ◽  
pp. 18-24
Author(s):  
Shashikala Kokcha ◽  
Harsha Chatrath

Purpose of the study: The purpose of this study is to make sure that the Bird valley’s quarry water is suitable for drinking purposes or not and to monitor the seasonal variations in the physico-chemical parameters of this quarry water. Methodology: Water samples from the quarry were collected in clean and sterilized polyethylene bottles. Water samples were collected from different points and mixed together to get an integrated sample. Some of the selected physico-chemical parameters of the quarry water have been analyzed. Results were compared with standard limits of IS: 10500-2012. All the parameters were analyzed in the laboratory by using standard methods and techniques. Main Findings: As per the obtained results this quarry water contains a very large number of Coliforms detected in the months of September and December and in June month Coliform count was 33 CFU/ml. This overall result for coliform is making this quarry water unfit for domestic purposes. Applications of this study: This study helps us to understand the current condition of this quarry water and also enables us to know whether the quarry water is fit for drinking purposes. It also enables us to know whether this quarry water can be used for domestic purposes after the treatment. Novelty/Originality of this study: Bird valley’s quarry water has not been analyzed from this point of view till date. This study will help us to understand the present condition of the water.


2014 ◽  
Vol 1 (2) ◽  
pp. 112-115
Author(s):  
Thenmozhi K ◽  
Karthika K ◽  
Manian S

The objective of the study is to cover the pharmacognostical and preliminary phytochemical screening of traditional medicinal plant, Kedrostis foetidissima belonging to the family Cucurbitaceae. This study includes organoleptic characters, physico-chemical analysis, fluorescence analysis, behaviour of leaf powder with different chemical reagents, phytochemical extraction, extractive yield and qualitative phytochemical screening. The preliminary screening revealed the presence of alkaloids, flavonoids, steroids, tannins, phenolics, glycosides, carbohydrates, proteins and aminoacids which explains that the plant must have valuable medicinal properties and so it can be explored


2019 ◽  
Vol 3 (02) ◽  
pp. 176-184
Author(s):  
Amit Kotiyal ◽  
Siddharth Shankar Bhatt ◽  
Jitendra Kumar ◽  
Dinesh Chandra Dimri ◽  
Narender Singh Mehta

The objective of this study was to compare the physico-chemical properties of 10 apple cultivars grown in mid hills of India. Fruit weight, volume, size, total soluble solids (T.S.S.), total sugars, titratable acidity and ascorbic acid content were measured in the cultivars Aurora, Brookfield, Braeburn, Galaxy, Azetec, Scarlet Gala, Marini Red, Jonagold, Royal Gala and Royal Delicious. The results showed significant differences in physico-chemical attributes of apple cultivars. Among all the cultivars the maximum fruit size (50.66 mm length, 74.73 mm diameter) and fruit weight (170.12 g) were observed in cv. Royal Delicious, while the minimum fruit size (40.52 mm length, 53.03 mm diameter) and weight (110.97 g) were measured in Azetec and Aurora, respectively. The highest volume (196.79 ml) of fruit was registered in Royal Delicious in comparison to the minimum (125.75 ml) in Aurora. The maximum T.S.S. (14.27 °B) and acidity (0.717%) was noticed in Scarlet Gala and Marini Red, respectively. While the minimum T.S.S. and acidity was observed in Marini Red (11.20 °B) and Azetec (0.186%). The ascorbic acid varied from 6.07 mg/100 g in Royal Gala to 9.86 mg/100 g in Braeburn, whereas the total sugar ranged 11.36% in Royal Gala to 7.06% in Jonagold.


2018 ◽  
Vol 3 (02) ◽  
pp. 150-157
Author(s):  
Asad Amir ◽  
Neelesh Kapoor ◽  
Hirdesh Kumar ◽  
Mohd. Tariq ◽  
Mohd. Asif Siddiqui

Sandalwood is a commercially and culturally important plant species belonging to the family Santalaceae and the genus Santalum. In Indian sandalwood is renowned for its oil, which is highly rated for its sweet, fragrant, persistent aroma and the fixative property which is highly demanded by the perfume industry. For better production and varieties, requires to understanding the functions of proteins, their analysis and characterization of proteins sequences and their structures, their localizations in cell and their interaction with other functional partner. Due to limited number of in silico studies on sandalwood, in the present study we have performed in silico analysis by characterization of sandalwood proteins. Total 23 proteins were obtained and characterization using UniProtKB, identifying their physico-chemical parameters using ProtParam tool and prediction of their secondary structure elements using GOR of all 23 proteins.


2016 ◽  
Vol 1 (01) ◽  
pp. 88-91
Author(s):  
Ram Kumar ◽  
M. M. Syamal ◽  
R. Chandra ◽  
Vishwanath .

The present investigation was conducted on different genotypes of aonla to assess the variability in physico- chemical properties of fruits. These plants were planted in the campus of Banaras Hindu University, Varanasi, U.P. The experiment was arranged in randomized block design with 12 treatments and 4 replications. The observations recorded during experiment showed that genotype 6 and 7 were found most superior, in terms of physico-chemical properties of fruits. The maximum pulp content (88.25%), pulp: stone ratio (8.24) and ascorbic acid content (679.25 mg/ 100g) was recorded in genotype 7. Whereas, highest TSS (12.18%), lowest acidity (1.80%) and maximum sugar (3.50%, 2.50% and 6.0% reducing, non-reducing and total sugar respectively) was found in genotype 6.


Author(s):  
Richard M Evans ◽  
Lilias Currie ◽  
Allan Campbell

The leucocyte ascorbic acid content is widely used as a measure of tissue ascorbic acid status. Standard methods of analysis, however, isolate both leucocytes and platelets (buffy layer), with consequent overestimation, since platelet ascorbic acid is attributed to the leucocytes. Fourteen healthy individuals on ascorbic acid supplements and 11 patients on mega dose ascorbic acid therapy were studied. A significant correlation was demonstrated between the ‘leucocyte’ ascorbic acid content and the platelet: leucocyte ratio (r = 0·70, p<0·001). It is suggested that changes in the relative distribution of platelets and leucocytes in the blood will result in an apparent change in the ‘leucocyte’ ascorbic acid content regardless of any actual change in the ascorbic acid content of the cells.


2017 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Kamyar Movagharnejad ◽  
Sepideh Pouya

Abstract— Drying is known as a food preservation method which increases the food’s storage time by water reduction. Traditional drying consisted of open sun-drying, but different industrial dryers have been widely used in recent times. The new dryers consist of convective, infrared, ultrasound, freeze fluidized bed and freeze dryers. All of these dryers reduce the water content but under different mechanisms which leads to the end products with different qualities. In this study we aim to compare the difference in quality of kiwi fruit slices dried by three different dryers: 1. Convective tray dryer, 2. Microwave dryer and 3. Freeze dryer. The tray dryer experiments were conducted in two air temperatures of 60 and 80oC in the constant air velocity of 0.8 m/s. The microwave dryer operated in 3 output powers of 180, 270 and 360 W. The condenser temperature and pressure in the freeze dryer reduced to -50oC and 0.1 mbar, respectively. The operating conditions and time were regulated so that the moisture content of all dried samples reduced to nearly 10% in the wet basis. The three parameters of color change, ascorbic acid and antioxidant reduction were selected as the measuring criteria for the comparison of the product qualities. The experiments show that the freeze drying caused the minimum color change while the microwave drying in the maximum power of 360W caused the maximum amount of color change. The concentration of ascorbic acid was measured in the fresh fruits and dried samples by standard methods. The measurements proved that the ascorbic acid content of the freeze dried samples was 80% of the fresh fruits. The ascorbic acid content of other samples was much lower. The antioxidant activity of the dried samples and the fresh fruits was also measured by standard methods and the experimental data also showed that the freeze drying causes the minimum reduction in the antioxidant activity.


Author(s):  
Priyanka Sharma

Introduction: Piper longum Linn, belonging to the family Piperaceae, is a climber, perennial shrub which is commonly found in India.  The dried fruits of Piper longum are widely used in Ayurvedic System of medicines since time unknown. It posses significant pharmacological properties due to presence of variety of chemical constituents in it. Objective: The present study is aimed to evaluate the pharmacognostical, physiochemical and phytochemical parameters for Piper longum fruits, as per the WHO guidelines for herbal drug standardization. Materials and Methods: WHO emphasized the use of standardized herbs and formulations for safety and best therapeutic results. Results and Discussion: Pharmacognostical studies shows that the fruits of Piper longum are found as green when fresh which turns grayish black upon drying. It posses pungent, bitter and acrid taste. The fruits are cylindrical with small petiole. The powder microscopy shows the presence of brown content, oleo resins, stone cells and calcium oxalate crystals. Physiochemical analysis shows variable extractive values in different solvents with maximum extractives of 20.6±0.021% in water and minimum extractive value of 6.6±0.036% in chloroform. Total Ash value of 8.3±0.015%, Acid Insoluble Ash- 1.16±0.025% and Water Soluble Ash- 5.5±0.01%, Foreign Matter- 1.62±0.12%, Moisture content of 15.70±0.051%, Bitterness value-1.96, Swelling Index-1.5±0.01 and Foaming Index- 102.33. Phytochemical analysis of the fruits of Piper longum shows the presence of alkaloids, volatile oil, tannins, fixed oils, and phenolics. Conclusion: The botanical, physical and chemical parameters obtained in this study can be used for establishing the identity and purity of the drug that will lead to safety and efficacy of the herb.


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