scholarly journals Antibiotic Residues and Microbial Contamination in Animal-Derived Foodstuffs in Pontianak City, Indonesia

2020 ◽  
Vol 15 (3) ◽  
pp. 152-161
Author(s):  
Yuli Arif Tribudi ◽  
Oke Anandika Lestari ◽  
Musa Alfius ◽  
Arif Hidayatullah
Keyword(s):  
Microbial Contamination ◽  
Chicken Meat ◽  
Coliform Bacteria ◽  
Animal Origin ◽  
Plate Count ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Beef Products ◽  
Microbial Contaminants ◽  
Pork Samples

Nowadays, demand for foods of animal origin is not limited in terms of quantity, but also the quality, nutritional value and safety for human consumption. Animal-derived foodstuffss can contain biological and chemical hazards through a contamination process. The aim of this study was to detect antibiotic residues and microbial contaminants in animal-derived foodstuffs, i.e. chicken meat, eggs, beef and pork. Samples were collected from different traditional markets (Flamboyan, Mawar, Kemuning and Dahlia) in Pontianak city, Indonesia. Antibiotic residues in foodstuffs were detected using the bioassay method against 4 classes of antibiotics (aminoglycoside, macrolide, penicillin and tetracycline), while the tested microbial contaminants included total plate count (TPC), Escherichia coli, Coliform and Salmonella sp.. Data obtained were analyzed using descriptive statistics. The results showed that antibiotic residues were only detected in chicken products. In chicken meat, 3 (60%) samples tested positive for tetracycline, while in chicken eggs, 3 (60%) and 5 (100%) samples tested positive for aminoglycoside and penicillin, respectively. As per the SNI 7388:2009, of the analyzed samples, 12 (100%) chicken meat, 6 (75%) beef and 5 (100%) pork samples exceeded the limit for TPC; 5 (41.67%) chicken meat and 5 (100%) pork samples exceeded the limit for both coliform bacteria and E. coli; and 7 (58.33%) chicken meat and 3 (37.5%) beef samples were positive for Salmonella. In conclusion, considerable levels of microbial contamination were detected in chicken and beef products in Pontianak city.

scholarly journals Antibiotic resistance and residue in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh

2021 ◽  
Vol 5 (1) ◽  
pp. 19-26
Author(s):  
Bidyut Matubber ◽  
Farzana Islam Rume ◽  
Mohammad Enamul Hoque Kayesh ◽  
Mohammad Mahfuzur Rahman ◽  
Mohammad Rohul Amin ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Chicken Meat ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Salmonella Spp ◽  
Resistance Patterns ◽  
Cattle Meat ◽  
Meat Samples ◽  
Controlling Measures ◽  
Antibiotic Resistance Patterns

The presence of antibiotic residue in chicken and animal meats is a serious threat to human health due to its harmful effects. This study aimed at identifying the antibiotic resistance patterns of the isolates as well as antibiotic residues in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh. A total of 205 meat samples, including 70 chicken meat, 60 cattle meat, 50 buffalo meat and 25 goat meat were aseptically collected and analysed for the detection of antibiotic residues by thin layer chromatography and the isolates obtained from these samples were subjected to antibiogram study against 16 commonly used antibiotics. The isolates found in this study were Staphylococcus spp., Streptococcus spp., Escherichia coli, and Salmonella spp. and their prevalence were 37.5% (77/205), 22.1% (48/205), 29.7% (61/205), 8.7% (19/205), respectively. The isolates showed different degrees of sensitivity to the antibiotics used in the study. The most resistant phenotype was against cefradine, amoxicillin, penicillin, oxytetracycline, erythromycin, and enrofloxacin. 18.5% (38/205) meat samples were found to be positive for antibiotic residues and the highest prevalence was observed in chicken meat compared to other meat types. Overall, the findings of the study suggest that it is important to take controlling measures for the emergence of antibiotic resistance and also for ensuring healthy meats for human consumption. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 19-26

scholarly journals Development and Optimization of an Amperometric Immunosensor for the Detection of Banned Antibiotic Residues in Honey

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 48
Author(s):  
Valérie Gaudin ◽  
Caroline Bodin ◽  
Céline Hédou ◽  
Christophe Soumet ◽  
Eric Verdon
Keyword(s):  
Electrochemical Method ◽  
Magnetic Beads ◽  
Matrix Effects ◽  
Antioxidant Activities ◽  
Simultaneous Detection ◽  
Animal Origin ◽  
Human Consumption ◽  
Screening Methods ◽  
Antibiotic Residues ◽  
The European Union

Veterinary drugs could contaminate animal-derived food products for human consumption. Some antibiotic residues (e.g., chloramphenicol (CAP), nitrofuran metabolites) are banned in foodstuffs of animal origin (e.g., milk, honey, etc.) in the European Union because of toxicological risks for the consumer. Screening methods applied for food safety monitoring should be sensitive, specific, cheap, quick, and portable for field testing (e.g., self-control). Electrochemical biosensors make it possible to develop a promising and economically interesting approach. An innovative and cheap electrochemical method based on disposable screen-printed carbon electrodes (SPCE), coupled to magnetic beads (MB), that allows the simultaneous detection of three families of antibiotics in milk was published by a Spanish academic team. When the biosensor method was applied to detect CAP residues in honey, two major issues were identified: firstly, the very low levels of residues to reach (i.e., regulatory limits below 1 µg/kg), and secondly, the complexity of the honey matrix; there is not a single honey matrix. Honey composition and color vary considerably depending on the botanical origin. Moreover, some honey ingredients can interfere with the electrochemical detection, especially substances with antioxidant activities (e.g., polyphenols). Therefore, in parallel with the optimization of the electrochemical method, the reduction of matrix effects was a big challenge.

scholarly journals Determination of Antibiotic Residues in Milk by Microbial Inhibitory Tests

2017 ◽  
Vol 61 (3) ◽  
pp. 57-64
Author(s):  
D. Juščáková ◽  
I. Kožárová
Keyword(s):  
Detection Sensitivity ◽  
Animal Origin ◽  
Cow’S Milk ◽  
Human Consumption ◽  
Grocery Stores ◽  
Feed Water ◽  
Antibiotic Residues ◽  
Cow's Milk ◽  
Raw Cow’S Milk

AbstractUndesirable substances enter the organism of animals mostly via feed, water or veterinary medicines and their residues pass subsequently into the products of animal origin. In dairy cows, sheep and goats these residues are eliminated particularly in milk. Milk intended for human consumption must comply with safety criteria also with respect to residues of antibiotics. The aim of this study was to determine the presence or absence of antibiotic residues in the milk using the tests Milchtest and Premi®Test. While the Milchtest was developed for the determination of antibiotic residues in cow, sheep and goat milk, the Premi®Test is intended for the determination of antibiotic residues in meat juice, liver, kidneys, fish, eggs and in the urine of animals treated with antibiotics. As examined matrices, we used 45 samples of raw cow’s milk collected at 3 agricultural farms and 10 samples of milk offered to consumers at grocery stores. When using the Milchtest, 8 samples tested positive and 10 provided dubious results while testing with the Premi®Test showed that only 6 samples were positive for antibiotics. Comparison of the results confirmed a higher detection sensitivity of Milchtest reflected in higher numbers of positive samples and the detection of dubious results in samples of raw cow’s milk. However, it should be noted that even the Premi®Test, although not intended preferably for the determination of antibiotics in milk, can be used, if needed, for the preliminary screening of antibiotic residues in such a matrix.

scholarly journals Impact of Antibiotics Residues on Food Microbiological Quality in Togo: Cases of Chicken Meat and Eggs

2020 ◽  
Author(s):  
Banfitebiyi GAMBOGOU ◽  
Abla Judith Estelle GNAMEY ◽  
Raouf T. MENSAH ◽  
Délia Carmen DIABANGOUAYA ◽  
Messanh KANGNI-DOSSOU ◽  
...  
Keyword(s):  
Food Security ◽  
Microbiological Quality ◽  
Chicken Meat ◽  
Test Method ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Bacteriological Quality ◽  
Antibiotics Residues ◽  
The Impact ◽  
Meat And Eggs

Abstract Purpose: The use of antibiotics in veterinary medicine has greatly promoted the intensification of animal production in recent decades. However, the improper use of these veterinary antibiotics causes their residues to be present in foodstuffs of animal origin and thus creates public health issues. Thus, the aim of this study is to assess the impact of the presence of antibiotic residues on bacteriological quality of meat and chicken eggs in Togo.Methodology: a survey was carried out among hundred chicken farmers in six markets in the city of Lomé. Then, AFNOR's routine method and the rapid Test method (Premi test) were used to assess the bacteriological quality and the presence of antibiotics in Chicken meats (54 samples) and eggs (36 samples). The statistical analysis is done with software SPSS v25.Results: The results of surveys of our operators have shown that broilers and laying hens are the most represented in the six markets. None of the interviewees ever received any training in biosecurity, food security and good hygiene practice. Out of 90 samples analyzed, 18.89% are positive for antibiotic residues with 99.51% of correlation between antibiotic residues and origin of the sample (p<0.05). Regarding microbiological quality, 16,7% (13/78) of compliance sample are positive to antibiotic residues and 33.3% (4/12) of no compliance sample are positive for antibiotic residues with 92.91% of correlation between microbiology quality and antibiotics residues (p <0.05).Conclusion: The presence of antibiotic residues in the chicken meat and eggs influence the detection of microorganisms. This leads to a food security problem in Togo.

scholarly journals Level of escherichia coli contamination of broiler chicken meat in Ambon City Market

2021 ◽  
Vol 883 (1) ◽  
pp. 012036
Author(s):  
I J Liur ◽  
M Veerman
Keyword(s):  
Broiler Chicken ◽  
Microbial Contamination ◽  
Sampling Technique ◽  
Chicken Meat ◽  
The Body ◽  
Plate Count ◽  
Broiler Meat ◽  
E Coli ◽  
Total Plate Count ◽  
Meat Markets

Abstract Chicken meat is a source of animal protein that is very popular with all people, especially in Indonesia. The meat also contains nutrients that are good for the body. However, broiler meat also has a weakness because it is a food that is easily damaged; most of the damage is caused by poor handling, thus providing opportunities for growth microbes. Broiler meat is easily contaminated by various microorganisms that are in the environment. This study aimed to determine the level of microbial contamination in broiler chicken meat in the Ambon city market. The study used a random sampling technique and used 12 samples from the market in Ambon city. Parameters of microbiologic status observed total plate count and E. coli. Data were described descriptively. The result showed that total plate count (TPC) and E-coli was not more than the standard. The microbial contamination of broiler chicken meat markets in Ambon city was not in excess with the National Standardization Agency (NSA, 2009). Thus, broiler meat is safe for consumption.

scholarly journals Knowledge and practice of cattle handlers on antibiotic residues in meat and milk in Kwara State, Northcentral Nigeria

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0257249
Author(s):  
Mary Idowu Olasoju ◽  
Taiwo Israel Olasoju ◽  
Oluwawemimo Oluseun Adebowale ◽  
Victoria Olusola Adetunji
Keyword(s):  
Online Survey ◽  
Animal Health ◽  
Health Concern ◽  
Animal Origin ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Cross Sectional ◽  
Poor Knowledge ◽  
Food Of Animal Origin ◽  
Knowledge And Practice

Objectives Antibiotics are important for improving animal health and production. However, the deposition of its residues in food of animal origin intended for human consumption at non-permissible levels has generated global health concern and the need to tackle this using the “One Health Approach”. This study assessed the knowledge and practice of 286 cattle handlers in Kwara State, Nigeria. Methods A web-based cross sectional online survey using a semi-structured questionnaire was conducted from November to December, 2019. Univariate, bivariate and multivariate analyses were performed at 95% confidence interval to determine predictors of good knowledge and practices towards Antibiotic Residues in Meat and Milk among cattle handlers. Results This study revealed that majority (52.7% n = 165/286) of the cattle handlers were not aware of antibiotic residues. Knowledge and practices regarding antibiotic residues were generally poor among the study population; 36.7% and 35.5% had satisfactory knowledge and practice respectively. The age (p = 0.026), gender (p = 0.006) and business duration (p = 0.001) of participants were significantly associated with their knowledge of antimicrobial residues. The effect of education on knowledge was modified by age. The odds of having poor knowledge on antibiotic residues increased 4 times among participants who were ≤40 years old than those above 40 years (Stratum Specific OR = 3.65; CI = 1.2, 11.1; p = 0.026). Knowledge levels of participants were statistically associated with their practice levels p<0.05 (OR = 2.43; CI = 1.45. 4.06; p = 0.0006). Conclusion This implies that poor knowledge is a risk factor to having poor practice among cattle handlers. Deliberate efforts towards educating cattle farmers on best farm practices in antibiotic use would prevent antibiotic residues in meat and milk. Also, an effective surveillance system for monitoring the use of veterinary drugs in Kwara State, Nigeria is crucial.

Evaluation of indicators for assessment of human and animal faecal pollution of surface run-off

1995 ◽  
Vol 31 (5-6) ◽  
pp. 235-241 ◽  
Author(s):  
P. Jagals ◽  
W. O. K. Grabow ◽  
J. C. de Villiers
Keyword(s):  
Informal Settlement ◽  
Dry Season ◽  
Faecal Coliform ◽  
Coliform Bacteria ◽  
Animal Origin ◽  
Human Consumption ◽  
Human Origin ◽  
Faecal Pollution ◽  
Run Off ◽  
Faecal Streptococci

The value of selected indicators for assessment of faecal pollution, as well as the distinction of faecal pollution of human or animal origin, has been investigated. The following indicators were included: faecal coliform bacteria, faecal streptococci, sorbitol-fermenting bifidobacteria, Rhodococcus coprophilus, somatic and male-specific coliphages, phages of Bacteroides fragilis, and cytopathogenic viruses. Comparative tests were carried out on samples collected from a stream and river exposed to predominantly faecal pollution of domestic animal origin, and the same stream and river after downstream exposure to run-off from a low socio-economic informal settlement with restricted sanitation. Samples were collected from perennial flow during the dry season and from stormwater run-off after thundershowers. Stormwater run-off from the settlement reached faecal coliform counts of up to 4 400 000 per 100 ml, which is equivalent to that of many raw sewage effluents. Faecal pollution was less during the dry season. Sorbitol-fermenting bidifobacteria were identifiable with faecal pollution of human origin, and R coprophilus bacteria with that of animal origin. The ratio of faecal coliforms to faecal streptococci was in the order of 3.5 to 4.7 immediately after exposure to sewage pollution of human origin. In water exposed to faecal pollution predominantly of animal origin, and downstream from pollution of human origin, this ratio varied from 0.8 to 1.7, which indicates that under circumstances the ratio may also distinguish between faecal pollution of human and animal origin. Phages of B fragilis and cytopathogenic viruses were not detected by direct titration in any of the samples, which implies that their application in this situation would require more sensitive techniques. The results show that the run-off from the informal settlement constituted a major source of pollution for a river catchment which downstream is used as a source of water for human consumption, and that faecal pollution of human and animal origin can reliably be distinguished by means of combinations of appropriate indicators.

scholarly journals Detection of Antibiotic Residues in Chicken Meat and Eggs from Traditional Markets at Yogyakarta City Using Bioassay Method

2020 ◽  
pp. 1-6
Author(s):  
Dyah Ayu Widiasih ◽  
Yatri Drastini ◽  
Doddi Yudhabuntara ◽  
F. Lintang R. Daru Maya ◽  
Prisha Lini Sivalingham ◽  
...  
Keyword(s):  
Chicken Meat ◽  
Animal Origin ◽  
Antibiotic Residues ◽  
Traditional Markets ◽  
Bioassay Method ◽  
Food Of Animal Origin ◽  
Meat Samples ◽  
Tetracycline Residues ◽  
Meat And Eggs ◽  
Yogyakarta City

Studies on antibiotic residues content in food of animal origin are currently needed to support veterinary public health programs. The present study was described bioassay method for the detection of antibiotic residues in chicken meat and eggs from traditional market at Yogyakarta City. A number of twenty-four chicken meat samples and 24 egg samples were taken from 8 traditional markets in Yogyakarta city. Samples were examined at Centre for Veterinary Wates, Yogyakarta, Indonesia using bioassay method for screening detection of penicillin, aminoglycoside, macrolide and tetracycline residues. This bioassay method using some bacteria, such as Bacillus stearothermophillus, B. cereus, B. subtilis, and Kocuria rizophila. A percentage of the results showed that 8.33% (2/24) samples of chickens tested positively contained the oxytetracycline antibiotic residues. Meanwhile, as much as 75% (18/24) samples of positive eggs contain penicillin antibiotic residues, positive residues of aminoglycoside amounted to 12.5% (3/24) and the positive residues of oxytetracycline also amounted to 12.5% (3/24).

scholarly journals Estimation of Microbial Contamination in Fresh and Commercial Fruit Juices: A Comparative Study

2021 ◽  
pp. 145-153
Author(s):  
Vanessa James ◽  
Hiral Panchal
Keyword(s):  
Staphylococcus Aureus ◽  
Microbial Contamination ◽  
Fruit Juice ◽  
Aloe Vera ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Plate Count ◽  
Human Consumption ◽  
Coliform Count ◽  
Total Plate Count

Aim and Objective: The objective of the present study is to determine microbial contamination in fresh and packaged commercial fruit juices (including a combination of Aloe vera with fruit juices) available in the Ahmedabad city of Gujarat, India. Materials and Methods: Seventeen samples were collected from various parts of the city which includes 9 commercial fruit juice samples and 8 street vended fresh fruit juice samples. Samples were examined for Total plate count, Yeast and mould count, coliform count, Escherichia coli, Salmonella, Staphylococcus aureus, Shigella, Enterobacteriaceae, Listeria monocytogens and Vibrio Cholerae. Results: Commercial fruit juices do not exceed the FSSAI standards for fruit juices and are free of harmful pathogens making themsafe for human consumption. Street vended fresh fruit juice samples exceedthe FSSAI limit for Total Plate count, Yeast and mould count and Coliform count. Street vended Fresh fruit juices demonstratethepresence of Ecoli, Salmonella and Staphylococcus aureus in 75% (6/8) samples. Enterobacteriaceae were identified in street vended fruit juices which exceeds the FSSAI standard limit. Conclusion: The study demonstrates that commercial fruit juices were safe for human consumption but fresh juices showed significant microbial growth and harmful pathogens which must be controlled to ensure consumer’s safety and health. However regular monitoring of commercial and fresh fruit juices is recommended to avoid food borne illness resulting from pathogens encountered in the study.

Effect of trisodium phosphate on the microbial contamination of chicken meat

2005 ◽  
Vol 34 (1) ◽  
pp. 5-11 ◽  
Author(s):  
L.A. Castillo A. ◽  
L. Mészáros ◽  
F. Kiss
Keyword(s):  
Microbial Contamination ◽  
Chicken Meat ◽  
Trisodium Phosphate
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