REMODELING PECULIARITIES OF ATRIAL ARTERY IN CONDITIONS OF POSTRESECTION PORTAL HYPERTENSION

It is known that structural and functional changes in the heart can be caused by various changes in hemodynamics, which include post resection portal hypertension. It should be noted that the structure of the atrial arteries in postresection portal hypertension has not been studied enough. The purpose of the study is to investigate the peculiarities of atrial artery remodeling in the conditions of post resection portal hypertension. Material and methods. The arteries of the atria of 34 male rats, which were divided into 2 groups, were studied by a complex of morphological methods. The group 1 consisted of 15 experimental intact animals; group 2 included 19 rats with post resection portal hypertension, which was simulated by removal of the left and right lateral lobes of the liver. Euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia one month after the start of the experiment. The outer and inner diameters of the atrial arteries of medium and small calibers, the thickness of the media and adventitial membrane, Wagenworth and Kernogan indices, the height of endothelial cells, the diameters of their nuclei, nuclear-cytoplasmic ratios in the studied endothelial cells, and relative volumes of damaged endotheliocytes were measured. Quantitative indicators were processed statistically. Results. It was established that in the conditions of post resection portal hypertension the arteries of small caliber of the atria changed more markedly. The outer diameter of these vessels of the left atrium increased by 4.2 %, the right - by 3.3 % (p <0.05), the thickness of the media and the adventitial membrane increased by 12.58 % and 45.7 % and respectively 11.8 % and 33.7 % (p <0.001, their lumen decreased by 13.5 % and 10.7 %, respectively) (p <0.001). The Wagenworth index of small caliber arteries increased markedly, and the Kernogan index decreased. Nuclear-cytoplasmic ratios in endotheliocytes of small arteries of the left atrium were changed by 7.6 %, and in the right - by 6.1 % (p<0.01), the relative volumes of damaged endotheliocytes respectively increased by 7.6 and 5, 2 times (p <0.001). Damage to a significant number of endothelial cells led to their dysfunction, blockade of NO synthase, decreased NO synthesis, activation of its degradation, which exacerbated spasm, narrowing of vessels and significantly impaired blood supply to the body and complicated by hypoxia.Histologically at post resection portal hypertension, there were pronounced vascular disorders, plethora, dilation of mainly venous vessels, perivasal edema and stroma edema, foci of dystrophic, necrobiotic, apoptotically altered endotheliocytes, stromal structures and cardiomyocytes. There was also swelling of endothelial cells, their dystrophy, necrobiosis, desquamation and proliferation. Conclusion. Post resection portal hypertension leads to severe remodeling of mainly small atrial arteries, which is characterized by thickening of their wall, narrowing of the lumen, changes in the Kernogan and Vaughanworth indices, endothelial cell damage, endothelial dysfunction, decreasing of organ blood supply, hypoxia, trophic disorders, dystrophic and necrobiolic changes of tissues and cells, infiltration, sclerosis.

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Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

10.21203/rs.2.24396/v3 ◽

2020 ◽

Author(s):

Sabin Bhandari ◽

Ruomei Li ◽

Jaione Simón-Santamaría ◽

Peter McCourt ◽

Steinar Daae Johansen ◽

...

Keyword(s):

Endothelial Cells ◽

Antigen Processing ◽

Constitutive Expression ◽

Cell Types ◽

The Body ◽

Male Rats ◽

Molecular Profile ◽

Immune Functions ◽

Liver Sinusoidal Endothelial Cells ◽

Sinusoidal Endothelial Cells

Abstract Background: Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs; liver resident macrophages) form the body´s most effective scavenger cell system for the removal of harmful blood-borne substances, ranging from modified self-proteins to pathogens and xenobiotics. Controversies in the literature regarding the LSEC phenotype pose a challenge when determining distinct functionalities of KCs and LSECs. This may be due to overlapping functions of the two cells, insufficient purification and/or identification of the cells, rapid dedifferentiation of LSECs in vitro, or species differences. We therefore characterized and quantitatively compared expressed gene products of freshly isolated, highly pure LSECs (fenestrated SE-1/FcgRIIb2+) and KCs (CD11b/c+) from Sprague Dawley, Crl:CD(SD), male rats using high throughput mRNA-sequencing and label-free proteomics.Results: We observed a robust correlation between the proteomes and transcriptomes of the two cell types. Integrative analysis of the global molecular profile demonstrated the immunological aspects of LSECs. The constitutive expression of several immune genes and corresponding proteins of LSECs bore some resemblance with the expression in macrophages. LSECs and KCs both expressed high levels of scavenger receptors (SR) and C-type lectins. Equivalent expression of SR-A1 (Msr1), mannose receptor (Mrc1), SR-B1 (Scarb1), and SR-B3 (Scarb2) suggested functional similarity between the two cell types, while functional distinction between the cells was evidenced by LSEC-specific expression of the SRs stabilin-1 (Stab1) and stabilin-2 (Stab2), and the C-type lectins LSECtin (Clec4g) and DC-SIGNR (Clec4m). Many immune regulatory factors were differentially expressed in LSECs and KCs, with one cell predominantly expressing a specific cytokine/chemokine and the other cell the cognate receptor, illustrating the complex cytokine milieu of the sinusoids. Both cells expressed genes and proteins involved in antigen processing and presentation, and lymphocyte co-stimulation. Conclusions: Our findings support complementary and partly overlapping scavenging and immune functions of LSECs and KCs. This highlights the importance of including LSECs in studies of liver immunity, and liver clearance and toxicity of large molecule drugs and nano-formulations.

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Variants of the anatomical structure of the coronary arteries and the left atrium remodeling in patients with different forms of atrial fibrillation

Clinical Medicine (Russian Journal) ◽

10.18821/0023-2149-2018-96-9-809-813 ◽

2018 ◽

Vol 96 (9) ◽

pp. 809-813

Author(s):

Nikita L. Sharikov ◽

S. M. Chibisov ◽

O. N. Ragozin ◽

S. Sh. Gasimova

Keyword(s):

Atrial Fibrillation ◽

Coronary Artery ◽

Left Atrium ◽

Blood Supply ◽

Right Coronary Artery ◽

Left Coronary Artery ◽

Sinoatrial Node ◽

Anatomical Structure ◽

Case Histories ◽

The Right

One of the reasons leading to dilatation of the left atrium is atrial fibrillation. A retrospective analysis of 136 case histories of patients with various forms of “non-valvular” atrial fibrillation was performed, depending on the shape of atrial fibrillation, the patients were divided into 3 groups. In patients with atrial fibrillation in 62.5%, the source of the atrial branches was the envelope branch of the left coronary artery. Atrial branches originating from the right coronary artery system were identified in 35.8%. In men, atrial arteries occur significantly more often. The results differ from publications, according to which the blood supply of the atria and sinoatrial node from 60 to 75% is carried out by branches departing from the basin of the right coronary artery. The degree of dilatation of the left atrium does not depend on the source of blood supply, but a correlation between the size of the left atrium and the diameter of the branches of the artery of the sinatrial node in the group of patients with paroxysmal atrial fibrillation is traced.

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Causes of sudden cardiac death in athletes

Medicinski pregled ◽

10.2298/mpns0702061p ◽

2007 ◽

Vol 60 (1-2) ◽

pp. 61-65

Author(s):

Dejana Popovic ◽

Miodrag Ostojic ◽

Nada Popovic ◽

Stanimir Stojiljkovic ◽

Ljiljana Scepanovic

Keyword(s):

Physical Activity ◽

Sudden Cardiac Death ◽

Cardiac Death ◽

Vigorous Physical Activity ◽

The Body ◽

Functional Changes ◽

Blunt Impact ◽

Dilatative Cardiomyopathy ◽

Physical Examinations ◽

The Right

Introduction Sudden cardiac death in athletes is a growing problem, despite the huge existing knowledge in medicine and sports. Effects of vigorous physical activity In response to vigorous physical activity, the body undergoes profound morphologic and functional changes. These changes are usually healthy, but sometimes may gravitate to some cardiac diseases. But still, most sudden cardiac deaths are due to previous unknown diseases. Causes of sudden cardiac death The most common cause of sudden cardiac death in athletes is hypertrophic cardiomyopathy. Other reasons are congenital coronary artery anomalies, myocarditis, dilatative cardiomyopathy, arrhythmogenic cardiomyopathy of the right ventricle, sarcoidosis, mitral valve prolapse, aortic valve stenosis, atherosclerosis, long QT syndrome, and blunt impact to the chest. Conclusion Bearing in mind the above mentioned, more frequent physical examinations of athletes are recommended.

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MORPHOMETRIC ANALYSIS REMODELING VESSELS HEMOMICROCIRCULATORY BED OF JEJUNUM AT RESECTIONS OF LIVER

Reports of Morphology ◽

10.31393/morphology-journal-2018-24(1)-03 ◽

2018 ◽

Vol 24 (1) ◽

pp. 16-20

Author(s):

M.S. Hnatjuk ◽

L.V. Tatarchuk

Keyword(s):

Portal Hypertension ◽

Varicose Veins ◽

Liver Parenchyma ◽

Male Rats ◽

Lateral Part ◽

The Difference ◽

The Right ◽

Bed Expansion ◽

Quantitative Indicators ◽

Sexually Mature

Liver resection is widely used in modern surgical departments. Removal of large volumes of the liver leads to postresection portal hypertension, which is complicated by bleeding from varicose veins of the esophagus, stomach, rectum, ascites, splenomegaly with secondary hypersplenism, parenchymal jaundice and portosystemic encephalopathy. The widespread prevalence of this pathology, high mortality from its complications indicates that it is an important medical and social problem. The aim of the study is quantitative morphological study of the features of remodeling the vessels of the hemomicrocirculatory bad of the jejunum at resection of different volumes of the liver. The studies were conducted on 45 sexually mature male rats, which were divided into 3 groups. The first group consisted of 15 intact virtually animals, 2-15 rats after resection of the left lateral part – 31.5% of the parenchyma of the liver, 3-15 animals after resection of the right and left lateral parts of the liver (58.1%). Euthanasia of experimental animals was carried out by bloodletting in conditions of thiopental anesthesia 1 month after the beginning of the experiment. The hemomicrocirculatory bad of the jejunum was studied by injection of its vessels into the carcass-gelatinous mixture, which was injected through the abdominal aorta. From the jejunum, lightened and histologic preparates were prepared and morphometrically studied the vessels of the microhemocirculatory bed. Quantitative indicators were processed statistically, the difference between the comparative values was determined by the Student's criterion. It was established that the severity of structural transformation of the vessels of the microhemocirculatory bad of the jejunum depends on the volume of the removed parenchyma of the liver. The analysis of the obtained results revealed that one month after resection of 31.5% of liver parenchyma, the morphometric parameters of the vessels of the haemomicrocirculatory bad of the jejunum were insignificantly altered. It was determined that resection of 58.1% of liver parenchyma caused postresection portal hypertension and marked remodeling of blood vessels of the hemomicrocirculatory bad of the jejunum, which was characterized by narrowing of arterioles by 23.4%, precapillary arterioles by 22.8%, hemocapillaries by 22.9%%, decrease in the density of microvessels – by 22.4%, the expansion of the capillary venules – by 35.5% and venules – by 28.7%, venous plethora, hypoxia, dystrophy, necrobiosis of cells and tissues, infiltrative processes and sclerosis. Resection of 58.1% of liver parenchyma leads to postresection portal hypertension, pronounced narrowing of lumen of the vessels of the adnexa and exchange units hemomicrocirculatory bed, expansion of the postcapillary venules and venules, venous plethora, hypoxia, dystrophy and necrobiosis of cells and tissues, infiltrative and sclerotic processes in wall of the jejunum.

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312 An Evaluation of the Clinical and Histological Effects of High Dose Radiosurgery on the Rat Dorsal Root Ganglion

Neurosurgery ◽

10.1093/neuros/nyx417.312 ◽

2017 ◽

Vol 64 (CN_suppl_1) ◽

pp. 266-267

Author(s):

Ezequiel Goldschmidt ◽

Wendy Fellows-Mayle ◽

Erin Paschel ◽

Ajay Niranjan ◽

John Flickinger ◽

...

Keyword(s):

Dorsal Root Ganglion ◽

Dorsal Root ◽

The Body ◽

Male Rats ◽

High Dose ◽

Sprague Dawley ◽

Histopathological Response ◽

Histological Techniques ◽

Sensory Deficits ◽

The Right

Abstract INTRODUCTION Stereotactic radiosurgery (SRS) is a safe and effective technique to create lesions of the brain and trigeminal nerve (TGN) in order to achieve neuromodulation. The lumbar dorsal root ganglion (DRG) contains the body of the sensory neurons responsible for pain sensitivity and can be targeted to treat chronic and debilitating pain in the extremities. Neuromodulation of the DRG might therefore improve chronic peripheral pain. This study was performed to determine the feasibility as well as clinical and histological effects of delivering high dose SRS targeted to the lumbar DRG in a rat model. METHODS Four Sprague Dawley male rats underwent 80 Gy maximum dose single-fraction SRS to the left L5 and L6 DRG using the Leksell Gamma Knife Icon (Elekta, Atlanta, GA) with onboard cone-beam CT imaging using 4 mm diameter collimators. The right L5 and L6 DRGs served as the controls. The animals were evaluated for motor and sensory deficits every two weeks. Two animals were sacrificed at 3 and two at 6 months after SRS. The lumbar spines were harvested and decalcified. Common histological techniques (Masson trichrome, Prussian blue) were used to assess for fibrosis and demyelination. RESULTS >No detectable motor or sensory deficits were seen in any animal. Histological changes including fibrosis and loss of myelin were noted to the left L5 and L6 DRGs, but not the right side control DRGs. Fibrotic changes within the vertebral body were also evident on the treated sides of the vertebral bodies. CONCLUSION We were able to detect a demyelinating histopathological response from SRS delivered to the DRG in rats. Since such changes mimic those seen after trigeminal SRS in experimental animals, we hypothesize that radiosurgery may be a potential option in chronic spinal radicular pain amenable to neuromodulation.

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Allevatori di uomini. Il caso dell'Istituto biotipologico ortogenetico di Genova

RIVISTA SPERIMENTALE DI FRENIATRIA ◽

10.3280/rsf2009-001003 ◽

2009 ◽

pp. 19-35

Author(s):

Emilio Maura ◽

F. Peloso Paolo

Keyword(s):

Public Health ◽

Early Diagnosis ◽

Catholic Church ◽

The Body ◽

Medical Tradition ◽

Medical Theory ◽

The Media ◽

The Right ◽

The University ◽

The Relationship

- The Biotypologic Orthogenetic Institute of the University of Genoa, was created, in 1926, by the Italian endocrinologist Nicola Pende (1880 -1970). Pende's biotypology follows the Italian medical tradition, fruit of two different trains of thought: Cesare Lombroso's medical approach and Achille De Giovanni and Giacinto Viola's constitutionalist theory. This dual line of thought brings medical scholars to focus on public health, early diagnosis and prevention, all topics comprising a political interest in society, nation and race. Moreover, this approach involves a reductionist view of the body/mind relationship - enclosing mental and relational life in the body - and consequently allows morphological and endocrinologic measurements. Pende's orthogenetics originates from the same premises as Eugenetics and adopts the same aims, but differs when it advocates the importance of acting after birth, so as not to infringe the tenets of the catholic church on the right of every person to live. Pende's medical theory - outlined before the fascist era - proposes a "total" and reductive approach to the complexity of the human being, in line with the fascists' endeavour to put each person in the right place (hence the usefulness of early diagnosis), and thus build, once and for ever, a perfect and stable social organisation. Pende's biotypology considers public health as a priority, followed by individual health. The past debate in the media - set off by the experience of Pende's Institute - addressed some issues discussed today : the relationship between individual and public health interests, and the bioethical features of early diagnosis in medicine and psychology. Keywords: biotypology, orthogenetics, biopolitics, constitutionalism, fascism, bioethics.

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Стан антиоксидантної системи експериментальних тварин при пошкодженні дванадцятипалої кишки за умов пострезекційної портальної гіпертензії

Medical and Clinical Chemistry ◽

10.11603/mcch.2410-681x.2017.v0.i4.8240 ◽

2018 ◽

Author(s):

M. S. Hnatjuk ◽

L. V. Tatarchuk

Keyword(s):

Portal Hypertension ◽

Epithelial Cells ◽

Antioxidant System ◽

Liver Parenchyma ◽

The State ◽

Male Rats ◽

Antioxidant Defence ◽

Experimental Animals ◽

Functional Changes ◽

Smooth Myocytes

Introduction. The resections of large volumes of the liver leads to postresection portal hypertension that results in structural-functional changes in the organs of portal vena system. The features of remodeling structures of duodenum and state of the antioxidant system of organism in the conditions of postresection portal hypertension are not studied yet. The aim of the study – to learn the state of the antioxidant system for experimental animals at the structural-functional changes of duodenum in the conditions of postresection portal hypertension. Research Methods. The studies were performed on 39 white male rats, which were divided into 3 groups. The first group consisted of 15 intact experimental animals (control), 2nd – 12 animals, which eliminated 42 % of liver parenchyma, 3rd – 12 rats after resection of 58.1 % of liver parenchyma. One month after the beginning of the experiment, euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia. In the blood of experimental animals, indicators characterizing the state of oxidative protection were determined – the activity of superoxidedismutase, catalase and ceruloplasmin. Sections of the duodenum were fixed in a 10 % neutral formalin solution and, after passing through the ethyl alcohol of increasing concentration, were placed in paraffin. Histologic sections 5–7 мm thick after deparaffinization were stained with hematoxylin-eosin, for van Gizon, Mallory, Weigert, and toluidine blue. Gistostereometrically determined the relative volumes of damaged endothelial cells, epithelial cells, smooth myocytes. A correlation analysis was carried out between biochemical and histogeometric indices with the definition of the coefficient (r) of correlation. Quantitative values were processed statistically. Results and Discussion. It is set that in the conditions of the modelled experiment activity of superoxidedismutase at the resection of 42 % parenchyma of liver statistically for certain (р<0.001) diminished on 15.5 % and at removal of 58.1 % parenchima of liver – on 25 %; activity of catalase – accordingly on 22.2 and 38.8 % and activity of ceruloplasmin – on 15.3 and 21.3 %. Received and the analyzed indexes testify that at resection of considerable volumes (42; 58.1 %) parenchyma of liver antioxidant defence of organism gets worse substantially and depends on removal at volumes of liver. A relative volume of the damaged endotheliocytes is in 2 to the group of supervisions with high authenticity (р<0.001) grew in 8.4 times, epithelial cells – in 18.9 times, and smooth myocytes – in 12 times. After the resection of 58.1 % parenchyma of liver the investigated morphometric parameters increased accordingly in 15.8; 31.4 and 18.1 times. Correlation connections between the indexes of antioxidant defence and relative at volumes of the damaged endotheliocytes, epithelial cells and smooth myocytes in duodenum at resection 42 % parenchima of liver were opposite moderate and significance (r=–0.48ч–0.56) at resection 58.1 % of liver – significance (r=–0.72÷-0.83). Conclusions. The obtained results indicate that antioxidant protection of the organism plays an important role in the adaptive-compensatory processes of the duodenum after resection of different volumes of liver parenchyma. The degree of antioxidant defense of the organism depends on the removed volume of liver parenchyma and the number of damaged structures of the duodenum.

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Morphometric aspects of studying the features of remodeling of atrial structures at postresection portal hypertension

Journal of Education, Health and Sport ◽

10.12775/jehs.2021.11.11.021 ◽

2021 ◽

Vol 11 (11) ◽

pp. 214-220

Author(s):

N. Gdanska ◽

M. Hnatjuk ◽

L. Tatarchuk ◽

N. Monastyrska

Keyword(s):

Portal Hypertension ◽

Left Atrium ◽

Morphological Changes ◽

Multiorgan Failure ◽

White Male ◽

Male Rats ◽

White Rats ◽

Left And Right ◽

Structural Homeostasis ◽

Right Atria

Introduction. Removal of the left and right lateral lobes of the liver in white rats leads to postresection portal hypertension, which is characterized by dilation and plethora of the hepatic portal vein, mesenteric veins, esophageal and gastric veins, veins of the anterior abdominal wall, splenomegaly, ascites and multiorgan failure. Objective of the research: to study the features of remodeling of the structures of the atria at postresection portal hypertension histostereometrically. Materials of the research and their discussion. The peculiarities of remodeling the structures of the atria were histostoreometrically studied in 65 adult white male rats, which were divided into 3 groups: the 1- consisted of 20 intact animals, the 2 – 30 rats with postresection portal hypertension, which was modeled by removal of the left and right lateral lobes of the liver, the 3 – 15 animals with postresection portal hypertension and multiorgan failure. One month after the start of the experiment, rats were euthanized by bloodletting under conditions of thiopental anesthesia. Histological micropreparations were used to determine the diameters of cardiomyocytes of the left and right atria, diameters of their nuclei, nuclear-cytoplasmic relations, relative volumes of cardiomyocytes, capillaries, stroma, stromal-cardiomyocyte, capillary-cardiomyocyte relations, relative volumes of damaged cardiomyocytes. Quantitative indicators were processed statistically. Results of the research and their discussion. Histostereometrically, it was found that postresection portal hypertrophy leads to hypertrophy of atrial cardiomyocytes, their nuclei, growth of stromal structures, relative volumes of damaged cardiomyocytes, disorders of tissue and cellular structural homeostasis. The revealed features of remodeling of the studied structures dominated in the left atrium at combination of postresection portal hypertension with multiorgan failure. Conclusions. Postresection portal hypertension leads to pronounced remodeling of the structures of the left and right atria, characterized by hypertrophy of cardiomyocytes, changes in their nuclear-cytoplasmic relations, an increase of the relative volume of stroma, damaged cardiomyocytes, pronounced disorders of tissue and cellular structural homeostasis. The revealed morphological changes dominated in the left atrium at combination of postresection portal hypertension with multiorgan failure.

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BIOELECTRIC IMPEDANCE OF THE BODY OF WISTAR RATS OF BOTH SEXES WITH A MONOCROTALINE MODEL OF PULMONARY ARTERIAL HYPERTENSION

Journal of Ural Medical Academic Science ◽

10.22138/2500-0918-2020-17-2-98-109 ◽

2020 ◽

Vol 17 (2) ◽

pp. 98-109

Author(s):

N.L. Kolomeyets ◽

◽

O.V. Syslonova ◽

S.L. Smirnova ◽

I.M. Roshchevskaya ◽

...

Keyword(s):

Pulmonary Hypertension ◽

Right Ventricle ◽

Amplitude Ratio ◽

Bioelectrical Impedance ◽

The Body ◽

Female Rats ◽

Male Rats ◽

High Current ◽

Bioelectric Impedance ◽

The Right

The aim of the work was to study the bioelectric impedance of the body in rats of both genders with monocrotaline-induced pulmonary hypertension. Materials and methods. Multi-frequency bioimpedance studies of the body of rats of both genders were carried out one month after the administration of monocrotaline (dose of 60 mg/kg, subcutaneously). Results. Hypertrophy of the right ventricle of the heart was revealed in animals with experimentally induced pulmonary hypertension, with a significantly greater thickness of the free wall of the right ventricle in males compared to females. Significant influence of the interaction of the gender factor and the effect of monocrotaline on the indicators of the bioelectric impedance of the body (amplitude, ratio of resistances at low and high current frequencies) was shown using the method of analysis of variance. The body bioelectric impedance amplitudes, normalized to body length, were significantly lower in the male rats and higher in the female rats with experimental pulmonary hypertension compared to the control animals of the same gender. In the female rats with the monocrotaline model of pulmonary hypertension, the ratio of the amplitudes of the bioelectric impedance of the body at low and high current frequencies was significantly lower than in the control animals. Conclusion. Changes in the body resistance of the rats with pulmonary hypertension indicate an inflammatory process. Gender differences in the changes in the bioelectrical impedance of the body indicate a more severe manifestation of pulmonary hypertension in the males.

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Assessment of sperm production and reproductive organs of Wistar rats to long-term exposure of Caesalpinia ferrea

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652010000400013 ◽

2010 ◽

Vol 82 (4) ◽

pp. 907-914 ◽

Cited By ~ 6

Author(s):

Leda M.F. Lucinda ◽

Camila B. Rocha ◽

Maycon M. Reboredo ◽

Vinícius C. Faria ◽

Rita C.S. Sá

Keyword(s):

Reproductive System ◽

Wistar Rats ◽

Wistar Rat ◽

Reproductive Organs ◽

The Body ◽

Male Rats ◽

Sperm Production ◽

Male Wistar Rats ◽

The Right

Caesalpinia ferrea Mart (Leguminosae) is a medicinal plant used to treat diabetes, among other therapeutic properties, but which is also reported to have hepatotoxic effects. Although it contains substances such as flavonoids and coumarin, which are known to have antifertility activity, no studies have apparently been conducted to evaluate the potential adverse side effects of this plant on the function of the reproductive system after a chronic treatment. Therefore, this investigation was carried out to evaluate the effect and safety of the long-term exposure to C. ferrea on male Wistar rats' vital organs, reproductive system and sperm production. Adult and immature male rats were treated with an aqueous extract of C. ferrea at a dose level of 300 mg/kg of body weight, administered during one or two spermatogenic cycles of this species. The reproductive and vital organs were analyzed, and sperm was collected from the epididymal secretion of the right epididymis cauda. The long-term administration of C. ferrea did not significantly alter the body, vital and reproductive organs weights. Gamete production was not affected either. The chronic assessment of C. ferrea suggests that this plant does not affect the normal functioning of the Wistar rat reproductive system.

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