scholarly journals Morphometric aspects of studying the features of remodeling of atrial structures at postresection portal hypertension

2021 ◽  
Vol 11 (11) ◽  
pp. 214-220
Author(s):  
N. Gdanska ◽  
M. Hnatjuk ◽  
L. Tatarchuk ◽  
N. Monastyrska
Keyword(s):  
Portal Hypertension ◽  
Left Atrium ◽  
Morphological Changes ◽  
Multiorgan Failure ◽  
White Male ◽  
Male Rats ◽  
White Rats ◽  
Left And Right ◽  
Structural Homeostasis ◽  
Right Atria

Introduction. Removal of the left and right lateral lobes of the liver in white rats leads to postresection portal hypertension, which is characterized by dilation and plethora of the hepatic portal vein, mesenteric veins, esophageal and gastric veins, veins of the anterior abdominal wall, splenomegaly, ascites and multiorgan failure. Objective of the research: to study the features of remodeling of the structures of the atria at postresection portal hypertension histostereometrically. Materials of the research and their discussion. The peculiarities of remodeling the structures of the atria were histostoreometrically studied in 65 adult white male rats, which were divided into 3 groups: the 1- consisted of 20 intact animals, the 2 – 30 rats with postresection portal hypertension, which was modeled by removal of the left and right lateral lobes of the liver, the 3 – 15 animals with postresection portal hypertension and multiorgan failure. One month after the start of the experiment, rats were euthanized by bloodletting under conditions of thiopental anesthesia. Histological micropreparations were used to determine the diameters of cardiomyocytes of the left and right atria, diameters of their nuclei, nuclear-cytoplasmic relations, relative volumes of cardiomyocytes, capillaries, stroma, stromal-cardiomyocyte, capillary-cardiomyocyte relations, relative volumes of damaged cardiomyocytes. Quantitative indicators were processed statistically. Results of the research and their discussion. Histostereometrically, it was found that postresection portal hypertrophy leads to hypertrophy of atrial cardiomyocytes, their nuclei, growth of stromal structures, relative volumes of damaged cardiomyocytes, disorders of tissue and cellular structural homeostasis. The revealed features of remodeling of the studied structures dominated in the left atrium at combination of postresection portal hypertension with multiorgan failure. Conclusions. Postresection portal hypertension leads to pronounced remodeling of the structures of the left and right atria, characterized by hypertrophy of cardiomyocytes, changes in their nuclear-cytoplasmic relations, an increase of the relative volume of stroma, damaged cardiomyocytes, pronounced disorders of tissue and cellular structural  homeostasis. The revealed morphological changes dominated in the left atrium at combination of postresection portal hypertension with multiorgan failure.

scholarly journals ALTERATION OF BRAIN TISSUE, LIVER, AND KIDNEY IN THE POST-CONTACT PERIOD IN WHITE RATS EXPOSED TO VIBRATION

2019 ◽  
Vol 98 (10) ◽  
pp. 1108-1112
Author(s):  
Eugeny A. Titov ◽  
V. A. Pankov ◽  
A. V. Lizarev ◽  
M. V. Kuleshova
Keyword(s):  
Brain Tissue ◽  
Liver Tissue ◽  
Morphological Changes ◽  
White Male ◽  
Experimental Studies ◽  
Kidney Tissue ◽  
Male Rats ◽  
Ultrastructural Changes ◽  
Contact Period ◽  
White Rats

Introduction. Experimental studies in animals have shown ultrastructural changes in hepatic sinusoidal endothelial cells, tissue hypoxia of the kidneys, changes in the activity of oxidative processes and antioxidant enzymes, the formation of bioenergetic hypoxia, cell response in the form of infiltration of both lymphoid and macrophage cells to develop due to exposure to vibration. However, there is almost no data about the status of animals’ organs in the post-contact period. Material and methods. The study was carried out in white male rats weighing 220-240 g, were exposed to 40 Hz vibration for 60 days 5 times a week for 4 hours a day. Histological and morphometric analysis was used to assess the sensorimotor cortex tissue and hepatorenal system. Results. A decrease in the total number of brain neurons, astraglial cells in rats in 30, 60 and 120 days of the post-exposure period was found. There was hyperemia in the portal and Central veins, an increase in the number of Kupffer cells in the liver tissue at the 30th, 60th, 120th day after the exposure. Decrease in the area of the Shumlyansky-Bowman capsule was recorded in the experimental rats’ kidney tissue 30 days after the end of vibration exposure; though there were no differences in the number of renal bodies in the tissue of white rats of the experimental and control groups at 60th and 120th day after the end of exposure to vibration. Conclusion. Morphological changes in the tissues of white rats exposed to prolonged vibration presented in the form of a decrease in the total number of neurons and astroglia cells in the brain tissue, a pronounced macrophage response in the liver tissue have been preserved in the post-contact period following the exposure to vibration.

scholarly journals Morphological Changes of the Thymus of the Juvenile White Rats Exposed to the Inhalation of Formaldehyde

2020 ◽  
Vol 9 (1) ◽  
pp. 16-23
Author(s):  
I. Yu. Vash
Keyword(s):  
Body Weight ◽  
Light Microscopy ◽  
Morphological Changes ◽  
White Male ◽  
Male Rats ◽  
Initial Body Weight ◽  
White Rats ◽  
Cortical Substance ◽  
Control Set ◽  
Number Of Cells

The aim of the article is to study the morphological features of the thymus of the white rats exposed to the inhalation of formaldehyde.Material and methods. 72 white male rats with an initial body weight of 130–150 g were were exposed to formaldehyde at a concentration of 2.766 mg/m3. Exposures were carried out once a day during 60 minutes. Exposure durations were 10, 20, 30, 60 and 90 days. We separately studied the thymus of rats, which, after 60 days of exposure to formaldehyde, were kept for 30 days in standard vivarium conditions (rehabilitation group). The structure of the thymus was studied by light microscopy.Results. The number of cells in an area of 2500 μm2 of the subcapsular zone in animals exposed to formaldehyde for 10, 20, and 30 days was 131.19±4.20, 135.65±7.74, and 135.35±10.83, respectively, which was less than the control set by 4.35%, 4.68% and 3.72%, respectively. After 40 and 60 days, this indicator was below the control by 8.90% and 9.63%.Conclusion. Inhalation of formaldehyde reduces the area of the thymus cortex. Phenomena of inversion of the cortex and the medulla are observed. Under the influence of formaldehyde, a picture of so called «starry sky» is observed in the cortical substance of the thymus. A decrease in the severity of changes in the thymus of rats belonging to the rehabilitation group, in comparison with animals that received 90 exposures, indicates that the changes are reversible.

THYMUS VARIABILITY IN WHITE RATS EXPOSED TO FORMALDEHYDE

2019 ◽  
pp. 105-116
Author(s):  
V.N. Voloshin ◽  
I.S. Voloshina ◽  
I.Yu. Vash
Keyword(s):  
White Male ◽  
Principal Component ◽  
Middle Part ◽  
Procrustes Analysis ◽  
Male Rats ◽  
Canonical Variables ◽  
Generalized Procrustes Analysis ◽  
White Rats ◽  
Main Component ◽  
Formaldehyde Inhalation

The aim of the paper is to study thymus variability in white rats, which were exposed to formaldehyde, and to compare these data with the indicators in control animals. Materials and Methods. The trial enrolled 72 white male rats, initial body weight 40–50 g. The animals were divided into 2 groups (36 rats in each). The first group consisted of control rats. Animals of the second group were exposed to formaldehyde inhalation, 2.766 mg/m3. To characterize the variability of the organ size, centroids were determined. The superposition of landmark configurations was performed using the generalized Procrustes analysis method, MorphoJ 1.06d program. The principal component analysis and canonical analysis of the obtained data were carried out. Results. One-Way ANOVA revealed a high level of intergroup differences in Procrust distance (F=1.34; p<0.0001). The significant effect of the duration of formaldehyde exposure on centroid size was established. The Kruskal-Wallis criterion was 19.778 (p=0.0014). The analysis of the principal components indicated that each of the first 10 components stands for more than 1 % of Procrustes coordinate variance. In this case, the first 7 components compatibly explain 91.398 % of thymus variability. The proportion of the first main component to the total variance of the Procrustes coordinates is 40.236 %. PC1 (-) shows changes in the thymus shape, mostly affecting the tops of its lobes, the middle part of the right boundary and the entire left thymus boundary. The scattering ellipses of the thymus ordinates in rats exposed to formaldehyde, in the first two canonical variables are located higher than those in the control animals. Conclusion. Formaldehyde inhalation leads to thymus changes in white rat. The most significant differences with control data are determined along the second canonical variable. Keywords: thymus, form, rat, formaldehyde, geometric morphometry. Цель. Изучение изменчивости формы тимуса белых крыс, находившихся в условиях влияния формальдегида, и сравнение этих данных с показателями, полученными у контрольных животных. Материалы и методы. Работа выполнена на 72 белых крысах-самцах с начальной массой тела 40–50 г. Животные были разделены на 2 серии (по 36 крыс). Первую серию составляли контрольные крысы. Животные второй серии подвергались ингаляционному воздействию формальдегида (ФА) в концентрации 2,766 мг/м3. Для характеристики изменчивости размеров органов определяли размер их центроидов. Процедуру суперимпозиции конфигураций ландмарок выполняли методом генерализованного прокрустова анализа с использованием программы MorphoJ 1.06d. Проводили анализ главных компонент и канонический анализ полученных данных. Результаты. Однофакторный дисперсионный анализ выявил высокий уровень межгрупповых различий по показателю прокрустовых расстояний (F=1,34; р<0,0001). Установлено значительное влияние продолжительности нахождения животных в условиях воздействия ФА на размер центроида. Критерий Краскела–Уоллиса составил 19,778 (р=0,0014). Анализ главных компонент указывал на то, что каждая из первых 10 компонент объясняет более 1 % дисперсии прокрустовых координат. При этом первые 7 компонент совместно объясняют 91,398 % изменчивости формы тимуса. Вклад первой главной компоненты в общую дисперсию прокрустовых координат составляет 40,236 %. РС1 (–) показывает изменения формы тимуса, в большей степени затрагивающие верхушки его долей, среднюю часть правого контура и весь левый контур тимуса. Эллипсы рассеивания ординат тимусов, принадлежащих крысам, подвергавшимся влиянию ФА, в пространстве первых двух канонических переменных расположены выше по отношению к таковым контрольных животных. Заключение. Ингаляционное воздействие формальдегида приводит к изменению формы тимуса белых крыс. Наибольшие различия с контрольными данными определяются вдоль второй канонической переменной. Ключевые слова: тимус, форма, крыса, формальдегид, геометрическая морфометрия.

scholarly journals Determination of the cumulative and skin-resorptive action of the Zoodizin disinfectant

2020 ◽  
Vol 22 (97) ◽  
pp. 26-30
Author(s):  
O. L. Nechyporenko ◽  
A. V. Berezovskyy ◽  
T. І. Fotina ◽  
R. V. Petrov
Keyword(s):  
Skin Irritation ◽  
White Male ◽  
Cumulative Effect ◽  
Female Rats ◽  
Male Rats ◽  
White Female ◽  
Test Substance ◽  
High Concentration ◽  
White Rats ◽  
Single Effect

The rational organization and implementation of effective disinfection activities plays an important role in the complex of measures for the prevention of infections. The development and introduction of new disinfectants into production is an urgent issue of modern poultry farming. When developing a disinfectant, it is important to determine the cumulative effect of the drug. The purpose of the study was to determine the cumulative and skin-resorptive action of the disinfectant “Zoоdizin”. For the toxicological study of the drug used healthy white male rats and white female rats weighing 200 ± 10 g 1.5 years of age. To study the toxicity of the drug “Zoоdizin” when applied to the skin used the method of immersion of the tails of rats in a test tube with the test substance. The tail was injected 2/3 into a regular tube with a 5 % solution of the drug “Zoоdizin”. The tube was closed with a cork ring whose diameter was slightly larger than the tail diameter. For 15 days, the tubes were placed daily in a water bath at 28–30 °C for 2 hours. Control animals tails were immersed in distilled water. To establish the local action of the drug “Zoodizin” on the mucous membranes of the study drug was introduced into the conjunctival sac of the right eye of the rabbit at a dose of 50 mg, and in the left eye was buried saline in a volume of 0,05 cm3. When studying the cumulative effect of Zoоdizin, no significant changes in the biochemical parameters in the serum of rats were observed. In the study of possible irritant or damaging effect on the skin and the development of contact non-allergic dermatitis found that a single application of disinfectant “Zoоdizin” on the unaffected skin of the back of white rats in the maximum significant recommended concentration of working solutions (2 %) did not cause signs. The single effect of the drug on the intact areas of the skin did not cause skin irritation, but it can be stated that prolonged daily epicutaneous exposure of high concentration (5 %) of the solution of the drug “Zoоdizin”, which is 2.5 times higher than the maximum recommended concentration, caused a general resorption. When assessing the cumulative properties, it was taken into account that the total dose administered to rats was Zodizin 42000 mg/kg body weight and did not result in animal death. It did not allow to calculate the cumulative coefficients for the “lethal effect”. A single effect of the product on the intact areas of the skin did not cause skin irritation, but it can be stated that prolonged daily epicutaneous exposure of a high concentration (5 %) of the Zodizin solution, which is 2.5 times the maximum recommended concentration, caused a general resorption. In the future, it is planned to study the virulidal properties of the biocide “Zoоdizin”.

scholarly journals Morphological features of the wall of common bile duct under the conditions of experimental opioid exposure

2020 ◽  
Vol 26 (2) ◽  
pp. 26-31
Author(s):  
L.R. Mateshuk-Vatseba ◽  
I.I. Hirniak ◽  
U.Y. Pidvalna
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Ducts ◽  
White Male ◽  
Acute Cholangitis ◽  
Medical Science ◽  
Male Rats ◽  
Pathological Changes ◽  
White Rats ◽  
The Common

The morphological condition of the bile ducts remains one of the most important problems of modern medical science. In order to obtain an analgesic effect in patients with acute cholangitis, opioids are often used. However, information on the effectiveness of opioids in the treatment of pathological conditions of the bile ducts is contradictory. The rapidly progressive destruction of the intrahepatic bile ducts associated with the use of narcotic agents has been described. Further study of the effect of opioids on the structural organization of the common bile duct is relevant. In order to establish the morphological state of the common bile duct under conditions of long-term opioid exposure, a study was performed on 24 sexually mature white male rats, aged 3.5-5.0 months and weighing 180-200 g, which were injected intramuscularly with Nalbuphine for 6 weeks. The study material is represented by histological specimens of the common bile duct of white rats. The “Aver Media” computer system was used to photograph microspecimens. The “ImageJ” computer program was used to measure the diameter of the lumen and the wall thickness of the common bile duct. After 2 weeks of Nalbuphine administration to white rats, plethora of wall microvessels and a significant increase in the longitudinal diameter of the lumen of the common bile duct were observed. After 4 weeks of the experiment, the common bile duct was dilated, the transverse and longitudinal diameters of its lumen almost doubled, pathological changes in its wall had all the signs of inflammation. In the later stages of the experiment (introduction of Nalbuphine for 6 weeks), the pathological changes increased and manifested by destructuring the wall of the common bile duct, disorganization of cholangiocytes, thinning of the cell layer due to detachment of cholangiocytes, polymorphism of their nuclei, destruction of intercellular junctions, stratification of its own plate, vacuolar dystrophy of the muscular membrane “varicose” expansion of venules, significant smooth muscle hyperplasia of arterioles, the presence of perivascular lymphocytic infiltrates in the duct wall.

scholarly journals THE PECULIARITIES LOCAL IMMUNE REACTIONS IN ILEUM AT POSTRESECTION PORTAL HYPERTENSION

2018 ◽  
Vol 17 (2) ◽  
pp. 71-75
Author(s):  
L. V. Tatarchuk ◽  
M. S. Hnatyuk
Keyword(s):  
Portal Hypertension ◽  
Mast Cells ◽  
Cell Density ◽  
Structural Changes ◽  
White Male ◽  
Liver Parenchyma ◽  
Male Rats ◽  
Secretory Iga ◽  
Immune Homeostasis ◽  
Immune Reactions

In the experiment, in laboratory white male rats, local immune reactions were investigated in the ileum in conditions of postresection portal hypertension, which was modeled by removing 58.1% of liver parenchyma. It has been established that postresection portal hypertension leads to pronounced changes in local immune reactions in the ileum, characterized by a 29.6% reduction in secretory IgA, a plasmoncytes with IgA on 1 mm² on the mucosa by 32.5%, an uneven, disproportional increase in the number of plasmatic cells with IgM. IgG, IgE, respectively, by 77.0%, 4.1 and 5.1 times. The cell density of the infiltrate in the mucosa of the investigated organ increased by 37.0%, and the number of mast cells by 35.8%. The results obtained indicate that postresection portal hypertension leads to weakening, tension and instability of the local immune homeostasis of the ileum mucosa and marked structural changes in the wall of the investigated organ.

scholarly journals Enterosgel role in neurodegenerative changes in the retina rats under the influence of Cr (VI) - induced retinopathy by study morphological changes in dynamic

2021 ◽  
pp. 14-20
Author(s):  
Olena Kuzenko ◽  
Yevhen Kuzenko ◽  
Yuri Demin
Keyword(s):  
Drinking Water ◽  
Morphological Changes ◽  
White Male ◽  
Degenerative Changes ◽  
Male Rats ◽  
Control Group ◽  
Fiber Layer ◽  
Experimental Conditions ◽  
Typical Structure ◽  
Ganglion Neurons

Chromium galvanic production have leaded to biosphere pollution. Therefore advisable to study of role in the neurodegenerative development in retinal diseases under experimental conditions. The aim is to study the Enterosgel effect on morphological changes in rats retina with Cr(VI) – induced retinopathy. Materials and methods. An experimental study had carried out on 72 outbred white male rats. The rats had divided into 3 groups: I – control group of intact rats (n = 24). Control rats were received drinking water, II group – rats (n = 24), were received drinking water with Cr (VI) (K2Cr2O7) – 0.02 mol/L, III group – animals (n = 24) were received drinking water with K2Cr2O7– 0.02 mol/L and hydrogel methylsilicic acid (Enterosgel) at a dose of 0.8 mg/kg per day as a corrector. The animals had been decapitated under ether anesthesia. The retina had been studied on days 20, 40 and 60 of the experiment. Morphologically and morphometrically they had analyzed. Results. According to histological studies, it has proved that Cr (VI) causes dystrophic and degenerative changes in all rats retina layers. They increase as the duration of the experiment. The use of Enterosgel as a corrective therapy showed positive results in restoring the morphological structure of rats retina. After Enterosgel 20 days using as a corrector of Cr (VI) exposure, there is a barely noticeable swelling of the outer and inner nuclear layers. Other layers of the retina, morphologically, look undamaged. Forty days Enterosgel treatment have outer and inner nuclear layer edema of retina of animals persists but does not increase. It is easy noticeable swelling of the outer and inner layers of mesh, but no signs of damage processes of cell populations nuclear layers. State ganglionic layer and nerve fiber layer entirely satisfactory. These pathological changes are not critical. After 60 days from the beginning of loading of Cr (VI) and application of Enterosgel in the retina of rats there are initial degenerative changes in the photosensory layer. Cystic dilated outer segments of rods and cones were visible throughout, and areas of their fragmentation were observed. Ganglion neurons are not damaged, but their axons appear somewhat thickened and fluffy. But in general, the typical structure of the retina is preserved. Conclusions. Chromium-induced toxicity in rats is characterized by pronounced histological and morphometric changes and retinal thickness, which appear after 20 days, increase by 40 days and acquire maximum transformations after 60 days of the experiment. The use of Enterosgel improves picture morphological structures of the retina in rats under the influence of Cr (VI). The changes were expressed on days 20 and 40, which indicates the presence of protective properties for the retina

scholarly journals REMODELING PECULIARITIES OF ATRIAL ARTERY IN CONDITIONS OF POSTRESECTION PORTAL HYPERTENSION

2021 ◽  
pp. 38-43
Author(s):  
М. S. Hnatjuk ◽  
N. M. Gdanska ◽  
L. V. Tatarchuk ◽  
Т. І. Gargula ◽  
О. В. Jasinovsky
Keyword(s):  
Endothelial Cells ◽  
Portal Hypertension ◽  
Left Atrium ◽  
Blood Supply ◽  
The Body ◽  
Male Rats ◽  
Outer Diameter ◽  
Functional Changes ◽  
The Media ◽  
The Right

It is known that structural and functional changes in the heart can be caused by various changes in hemodynamics, which include post resection portal hypertension. It should be noted that the structure of the atrial arteries in postresection portal hypertension has not been studied enough. The purpose of the study is to investigate the peculiarities of atrial artery remodeling in the conditions of post resection portal hypertension. Material and methods. The arteries of the atria of 34 male rats, which were divided into 2 groups, were studied by a complex of morphological methods. The group 1 consisted of 15 experimental intact animals; group 2 included 19 rats with post resection portal hypertension, which was simulated by removal of the left and right lateral lobes of the liver. Euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia one month after the start of the experiment. The outer and inner diameters of the atrial arteries of medium and small calibers, the thickness of the media and adventitial membrane, Wagenworth and Kernogan indices, the height of endothelial cells, the diameters of their nuclei, nuclear-cytoplasmic ratios in the studied endothelial cells, and relative volumes of damaged endotheliocytes were measured. Quantitative indicators were processed statistically. Results. It was established that in the conditions of post resection portal hypertension the arteries of small caliber of the atria changed more markedly. The outer diameter of these vessels of the left atrium increased by 4.2 %, the right - by 3.3 % (p <0.05), the thickness of the media and the adventitial membrane increased by 12.58 % and 45.7 % and respectively 11.8 % and 33.7 % (p <0.001, their lumen decreased by 13.5 % and 10.7 %, respectively) (p <0.001). The Wagenworth index of small caliber arteries increased markedly, and the Kernogan index decreased. Nuclear-cytoplasmic ratios in endotheliocytes of small arteries of the left atrium were changed by 7.6 %, and in the right - by 6.1 % (p<0.01), the relative volumes of damaged endotheliocytes respectively increased by 7.6 and 5, 2 times (p <0.001). Damage to a significant number of endothelial cells led to their dysfunction, blockade of NO synthase, decreased NO synthesis, activation of its degradation, which exacerbated spasm, narrowing of vessels and significantly impaired blood supply to the body and complicated by hypoxia.Histologically at post resection portal hypertension, there were pronounced vascular disorders, plethora, dilation of mainly venous vessels, perivasal edema and stroma edema, foci of dystrophic, necrobiotic, apoptotically altered endotheliocytes, stromal structures and cardiomyocytes. There was also swelling of endothelial cells, their dystrophy, necrobiosis, desquamation and proliferation. Conclusion. Post resection portal hypertension leads to severe remodeling of mainly small atrial arteries, which is characterized by thickening of their wall, narrowing of the lumen, changes in the Kernogan and Vaughanworth indices, endothelial cell damage, endothelial dysfunction, decreasing of organ blood supply, hypoxia, trophic disorders, dystrophic and necrobiolic changes of tissues and cells, infiltration, sclerosis.

Effects of hyperthyroidism on delayed rectifier K+ currents in left and right murine atria

2005 ◽  
Vol 289 (4) ◽  
pp. H1448-H1455 ◽  
Author(s):  
Ying Hu ◽  
S. V. Penelope Jones ◽  
Wolfgang H. Dillmann
Keyword(s):  
Protein Expression ◽  
Left Atrium ◽  
Right Atrial ◽  
Delayed Rectifier ◽  
Rnase Protection ◽  
Expression Levels ◽  
Mrna And Protein Expression ◽  
Left And Right ◽  
The Right ◽  
Right Atria

Hyperthyroidism has been associated with atrial fibrillation (AF); however, hyperthyroidism-induced ion channel changes that may predispose to AF have not been fully elucidated. To understand the electrophysiological changes that occur in left and right atria with hyperthyroidism, the patch-clamp technique was used to compare action potential duration (APD) and whole cell currents in myocytes from left and right atria from both control and hyperthyroid mice. Additionally, RNase protection assays and immunoblotting were performed to evaluate the mRNA and protein expression levels of K+ channel α-subunits in left and right atria. The results showed that 1) in control mice, the APD was shorter and the ultra-rapid delayed rectifier K+ conductance ( IKur) and the sustained delayed rectifier K+ conductance ( Iss) were larger in the left than in the right atrium; also, mRNA and protein expression levels of Kv1.5 and Kv2.1 were higher in the left atrium; 2) in hyperthyroid mice, the APD was shortened and IKur and Iss were increased in both left and right atrial myocytes, and the protein expression levels of Kv1.5 and Kv2.1 were increased significantly in both atria; and 3) the influence of hyperthyroidism on APD and delayed rectifier K+ currents was more prominent in right than in left atrium, which minimized the interatrial APD difference. In conclusion, hyperthyroidism resulted in more significant APD shortening and greater delayed rectifier K+ current increases in the right vs. the left atrium, which can contribute to the propensity for atrial arrhythmia in hyperthyroid heart.

scholarly journals Features of angioarchitectonics and structure of the aortic wall of white rats in the early stages of experimental diabetes mellitus

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 167-174
Author(s):  
M.N. Tsytovskyi ◽  
M.V. Logash ◽  
I.I. Savka ◽  
G.M. Dmytriv
Keyword(s):  
Diabetes Mellitus ◽  
Aortic Wall ◽  
Experimental Diabetes ◽  
White Male ◽  
Male Rats ◽  
White Rats ◽  
Significant Difference ◽  
Streptozotocin Induced Diabetes ◽  
Atherosclerotic Cardiovascular Diseases ◽  
Imagej Software

Background. Atherosclerotic cardiovascular diseases, as well as coronary heart disease, cerebrovascular disease, and peripheral artery disease (probably caused by atherosclerosis), are the leading cause of disability and mortality in people with diabetes. Objective. The purpose of our study was to determine the histostructural features and morphometric analysis of the components of the aortic wall and its hemomicrocirculatory bed after 2 and 4 weeks of streptozotocin-induced diabetes mellitus. Methods. The samples for the histology were the sections of the wall of the ascending part, the aortic arch, and the descending part of the aorta of 26 mature white male rats weighing 100 - 160 g. For morphometric examination, a series of images of the aortic wall was taken using a Meiji MT4300 LED microscope with an x40 objective, x10 eyepiece. Results and conclusion. The measurements were carried out using the ImageJ software. The development of micro- and macroangiopathies in experimental animals with 8-week streptozotocin-induced diabetes mellitus was histologically proved. Statistical analysis revealed a significant difference of all morphometric parameters of both - the components of the aortic wall and the vessels of its hemomicrocirculatory bed after 4 weeks of experimental diabetes in comparison with the norm, control, and the 2-nd week of the experiment. An explicit dependency of the severity of destructive changes in the wall of the aorta and links of its hemomicrocirculatory bed of vessels on the duration of the experiment was determined.

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