EFFECT OF LEAD NITRATE ON THE TESTIS OF ADULT WISTAR RATS AT DIFFERENT DOSAGE

2016 ◽  
Vol 2 (1) ◽  
pp. 5
Author(s):  
Richard Agbonluai EHIMIGBAI
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Lead Nitrate ◽  
Normal Diet ◽  
Seminiferous Tubules ◽  
Control Groups ◽  
Orogastric Tube ◽  
Group A ◽  
Group B ◽  
Group D

Introduction The aim of this study was aimed at evaluating the effects of lead nitrate on adult wistar rats.Methodology A total of 32 adult wistar rats weighing between 200 and 240g were divided into 4 groups of 8 rats per group. The control groups (group A) were placed on normal diet, while Group B rats received 35mg/ kg body weight / day of lead nitrate via orogastric tube. Group C rats received 45mg / kg body weight/ day of lead nitrate via orogastric tube. Group D rats received 50 mg/ kg body weight/ day of lead nitrate via orogastric tube and all the dosage were given for 40 days.Results The results in group A, B, C and D showed normal histologic testicular architecture as evidenced by normal seminiferous tubules and interstitial spaces in figure 1, 2, 3 and 4.Conclusion It was concluded that lead nitrate at 35mg/kg, 45mg/kg and 50mg/kg body weight/day was not toxic to the testis of an adult Wistar rats. 

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals Histological Assessment on Cadmium Induced Liver Damage Following Oral Administration of Moringa oleifera Oil and Anarcardium occidentale Nuts Oil in Wistar Rats

2019 ◽  
pp. 1-8
Author(s):  
O. D. Omotoso ◽  
S. A. Adelakun ◽  
O. O. Oyewo ◽  
B. T. Adediran
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Central Vein ◽  
Group A ◽  
Moringa Oleifera Oil ◽  
Group B ◽  
Group D

Background: Cadmium is a human carcinogen element and one of the most toxic pollutants in the environment. Present study aims at histomorphological study of some of the effects of Moringa oleifera oil and cashew (Anarcardium occidentale) nuts oil on Cadmium-induced liver damage in wistar rats. Methods: Thirty-five Wistar rats (80-180 g) were randomly selected and divided into seven groups of five rats each after acclimatization for two weeks. Group A which served as control received phosphate buffer, Group B received Cadmium only (3.5 mg/kg body weight). Group C received Vitamin C and Vitamin E; Group D received Vitamin C, Vitamin E and Cadmium. Group E received Cadmium and Moringa oleifera oil, Group F received Cadmium and Cashew nut oil and Group G received Cadmium (3.5 mg/kg body weight) and Moringa oleifera oil and Cashew (Anarcardium occidentale) nut oil. Results: Histological examination revealed normal histo-architecture of the liver, the central vein and the hepatocytes were seen clearly and there was orderly arrangement of the hepatocytes in Group A (control group). In group B, there was enlarged portal vein and there was distorted arrangement of the hepatocytes. In group C, the hepatocytes and the central vein were seen. In group D, the central vein and hepatocytes were seen though not orderly arranged. In groups E, F and G, there was restoration of the hepatocytes and the central vein was seen. Conclusion: This therefore suggests that Moringa oleifera oil and Cashew (Anarcardium occidentale) nut oil have ameliorative effects which led to the restoration of the damaged and distorted hepatocytes.

scholarly journals Histological studies on the retina and cerebellum of Wistar rats treated with Arteether™

2014 ◽  
Vol 31 (01) ◽  
pp. 028-032
Author(s):  
A. Okunlola ◽  
C. Okunlola ◽  
C. Okani ◽  
O. Adewole ◽  
D. Ofusori ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Study Group ◽  
Group A ◽  
Cervical Dislocation ◽  
Nissl Stain ◽  
Group B ◽  
Haematoxylin And Eosin Stain ◽  
Therapeutic Doses ◽  
Cellular Components ◽  
Group D

Abstract Introduction: Arteether™, a derivative of artemisinin, is among the recent drugs that have given renewed hope for combating malarial menace. The present study investigated the effects of arteether™ on the histology of the retina and cerebellum of Wistar rats. Materials and Methods: Twenty adult albino Wistar rats weighing 150-200 g, were randomly divided into four groups (A, B, C and D) of five animals each and used for this study. Group A rats were given intramuscular (i.m.) arteether™ (3 mg/kg b.w.) daily for 3 days. Group B rats were given i.m. arteether™ (6 mg/kg b.w.) daily for 3 days. Group C rats were also given i. m. of arteether™ (3 mg/kg b. w.) daily for 3 days, and the same dose was repeated at two-weekly intervals for 4 further weeks; while Group D rats which received normal saline (0.9 % w/v, 3 ml/kg b.w.), served as controls. At the end of the experiment, the rats were sacrificed by cervical dislocation. The retina and cerebellum were excised and processed routinely for histopathology changes, using haematoxylin and eosin stain (H & E), as well as Nissl stain. Results: Results obtained showed normal cellular components of the retina and cerebellum in all groups, and no cyto-pathological changes were observed. Conclusion: Thus, this study showed that under light microscopic examination, therapeutic doses of arteether™ caused no significant cyto-pathologic changes in the retina and cerebellum of Wistar rats.

scholarly journals Pengaruh pemberian ekstrak daun cengkeh (Syzygium aromaticum) terhadap gambaran histopatologik hati tikus wistar yang diberikan parasetamol dosis toksik

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Denty M. Sidabutar ◽  
Carla F. Kairupan ◽  
Meilany Durry
Keyword(s):  
Single Dose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Cell Damage ◽  
Liver Cells ◽  
Syzygium Aromaticum ◽  
Toxic Dose ◽  
Group A ◽  
Group B ◽  
Group D

Abstract: Clove (Syzygium aromaticum) contains eugenol, a phenolic compound, which has been suggested to possess antioxidant activity. This compound is suspected to be able to minimize damage to the liver cells caused by drugs such as paracetamol. This study aimed to observe the effects of clove leaf extract on histopathological features of Wistar rat liver tissue induced with toxic doses of paracetamol. This was an experimental laboratory study. Subjects were 24 male Wistar rats. The dose of clove leaf extract was 200 mg/day (single dose) and of paracetamol 50 mg/day (single dose) orally. Group A (negative control) was given no treatment for 14 days. Group B was given paracetamol for 14 days. Group C was given clove leaf extract for 7 days and then added with paracetamol for 7 days. Group D was given clove leaf extract and paracetamol simultaneously for 14 days. Group A showed normal histological feature of liver cells. Group B showed liver cell damage induced by paracetamol. Group C showed regeneration of liver cells, but there were still some necrosis and fatty liver cells. Group D showed regeneration of liver cells meanwhile cell necrosis was hardly found. Conclusion: Clove leaf extract could improve the histopathological changes of liver tissues of Wistar rats due to administration of paracetamol at toxic dose. This improvement was manifested as better regeneration of liver cells than that of rats not treated with clover leaf extract. Keywords: clove, paracetamol, liver Abstrak: Cengkeh (Syzygium aromaticum) mengandung senyawa eugenol, suatu komponen fenolik yang memiliki aktivitas antioksidan. Senyawa eugenol diduga dapat meminimalisir kerusakan sel hati yang antara lain disebabkan oleh obat-obatan yang berefek hepatototoksik seperti parasetamol. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun cengkeh terhadap gambaran histopatologik hati tikus wistar yang diinduksi dengan parasetamol dosis toksik. Jenis penelitian ini eksperimental laboratorik. Subjek penelitian 24 ekor tikus wistar. Pada penelitian ini digunakan ekstrak daun cengkeh 200 mg/hari (dosis tunggal) dan obat parasetamol 50 mg/hari (dosis tunggal) per oral. Subjek penelitian dibagi menjadi 4 kelompok. Kelompok A (kontrol negatif) tidak diberi perlakuan selama 14 hari. Kelompok B diberikan parasetamol selama 14 hari. Kelompok C diberikan ekstrak daun cengkeh selama 7 hari kemudian ditambahkan parasetamol secara bersamaan selama 7 hari. Kelompok D diberikan ekstrak daun cengkeh dan parasetamol secara bersamaan selama 14 hari. Kelompok A memperlihatkan gambaran histopatologik sel hati normal. Kelompok B memperlihatkan kerusakan sel hati berupa nekrosis dan perlemakan sel. Kelompok C memperlihatkan regenerasi sel hati namun masih terdapat nekrosis dan perlemakan sel hati. Kelompok D memperlihatkan regenerasi sel hati yang luas dan hampir tidak ditemukan nekrosis sel. Simpulan: Pemberian ekstrak daun cengkeh memperlihatkan perbaikan gambaran histopatologik jaringan hati tikus wistar yang mengalami kerusakan akibat parasetamol dosis toksik berupa regenerasi sel hati yang lebih baik dibandingkan dengan yang terlihat pada jaringan hati tikus wistar yang tidak diberi ekstrak daun cengkeh.Kata kunci: cengkeh, parasetamol, hati

scholarly journals Gestational and neurodevelopmental effects of black mustard seeds' (Brassica nigra) extract in wistar rats

1970 ◽  
Vol 8 (1) ◽  
pp. 1368-1378
Author(s):  
Bernard Ufuoma Enaibe ◽  
Tolulope Timothy Arogundade ◽  
Oluwaseun Adigun ◽  
Foyeke Munirat Adigun ◽  
Emmanuel Olusola Yawson ◽  
...  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Brassica Nigra ◽  
Female Rats ◽  
Rat Pups ◽  
Group A ◽  
Black Mustard ◽  
Group B ◽  
Mustard Seeds

This study investigated the effect of the crude aqueous extract of Brassica nigra (Black Mustard Seeds) in gestation and on the prefrontal cortex of newborn Wistar rats at different doses following prenatal administration. Eighteen (18) adult female rats weighing an average of 180±10g were used. The female rats were split into 3 groups of six animals; Group A received distilled water throughout gestation, Group B received 200 mg/kg body weight of extract throughout gestation, and Group C received 100 mg/kg body weight of extract throughout gestation). Rat pups from the experimental groups were sacrificed on postnatal days 1, 7, 14, 21, 28, and 35 and subsequently prepared through routine histological and histochemical procedures. Brassica nigra was abortifacient at 200 mg/kg body weight and reduced litter size at 100 mg/kg body weight. No observed physical deformities in pups of treated groups. Comparative prefrontal microarchitecture revealed little to no alteration in the treated group. This study concludes that Brassica nigra (black mustard) is not totally innocuous and as such, should be moderately consumed or totally avoided in pregnancy.Keywords: Brassica nigra; Mustard seeds; Gestation; Neurodevelopment.

scholarly journals EFEK PEMBERIAN ANABOLIK ANDROGENIK STEROID INJEKSI DOSIS RENDAH DAN TINGGI TERHADAP GAMBARAN HISTOPATOLOGI HATI DAN OTOT RANGKA TIKUS WISTAR (Rattus Novergicus)

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Pamela K. Sari ◽  
Poppy M. Lintong ◽  
Lily L. Loho
Keyword(s):  
Protein Synthesis ◽  
Wistar Rats ◽  
Low Dose ◽  
Anabolic Steroids ◽  
Inflammatory Cells ◽  
High Dose ◽  
Group A ◽  
Androgenic Anabolic Steroids ◽  
Group B ◽  
Group D

Abstract: Androgenic-anabolic steroids (AAS) are synthetic derivatives of the male hormone endogenous testosterone that stimulates anabolic (protein synthesis) and androgenic effects (masculinization). Long-term usage of AAS can result in liver damage. However, physiological concentrations of testosterone can stimulate protein synthesis which lead to an increase in muscle size, body mass, and endurance. This study aimed to determine the histopathology of liver and skeletal muscles of wistar rats that were given low dose and high dose injection of AAS. Subjects were 21 wistar rats divided into 7 groups. Group A was given standard pellets for 56 days (negative control), terminated on days 29,43, and 57. Group B was treated with low-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group C was treated with low-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group D was treated with low-dose AAS injection and standard pellets for 56 days, terminated on day 57. Group E was treated with high-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group F was treated with high-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group G was treated with high-dose AAS injection and standard pellets for 56 days, terminated on day 57. The results showed that the histopathology of liver and muscles in group A was still normal. In group B, the architecture of liver was still normal with a few inflammatory cells around the Kiernan triangle while in muscle the ratio of myofibril diameter was 1.28:1. In group C and group D, there were widening of the hepatic artery, bile duct, and portal vein containing blood fibrin, and inflammatory cells around the Kiernan triangle. The ratio of myofibril diameter was 1.43:1 in group C and 2.14:1 in group D. In group E, F and G, there were micro-vesicular fatty cells in the peripheral part of the liver meanwhile the myofibril diameter ratio of the muscles in group E was 1.43:1, group F 2.1:1, and group G 2.28:1. Conclusion: Administration of AAS injection of low dose and high dose for less than 4 weeks could result in inflammation, dilation of the portal vein, hepatic artery and bile duct meanwhile administration of AAS for over 4 weeks could ressult in focal fatty liver (steatosis). The administration of AAS injection of low dose and high dose for 4,6 and 8 weeks reslutid in enlargement of skeletal muscle (muscle hypertrophy).Keywords: androgenic-anabolic steroids, liver, skeletal muscleAbstrak: Anabolik Androgenik Steroid (AAS) adalah derivat sintetis dari hormon sex testosteron endogen pria, yang merangsang efek anabolik (sintesis protein) dan androgenik (maskulinisasi). Penggunaan AAS jangka panjang dapat mengakibatkan terjadinya kerusakan hati namun secara fisiologi testosteron dapat menstimulasi sintesis protein sehinggaberdampak pada peningkatan ukuran otot, massa tubuh dan ketahanan tubuh. Penelitian ini bertujuan untuk mengetahui gambaran histopatologi hati dan otot rangka wistar yang diberikan AAS injeksi dosis rendah dan dosis tinggi. Subjek penelitian 21 ekor wistar yang dibagi menjadi 7 kelompok. Kelompok A diberi pelet standar selama 56 hari (kontrol negatif), terminasi pada hari ke-29, 43, dan 57. Kelompok B diberi perlakuan AAS injeksi dosis rendah dan pelet standar selama 28 hari, terminasi hari ke-29. Kelompok C diberi AAS injeksi dosis rendah dan pelet standar selama 42 hari, terminasi hari ke-43. Kelompok D diberi AAS injeksi dosis rendah dan pelet standar selama 56 hari, terminasi hari ke-57. Kelompok E diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 28 hari, terminasi hari ke-29. Kelompok F diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 42 hari, terminasi hari ke-43. Kelompok G diberi perlakuan AAS injeksi dosis tinggi dan diberi pelet standar selama 56 hari, terminasi hari ke-57. Hasil penelitian menunjukkan pada kelompok A didapatkan gambaran histopatologi hati normal sedangkan pada otot tidak terdapat perubahan. Pada kelompok B didapatkan arsitektur hati masih normal dengan sedikit sel radang disekitar segitiga Kiernan sedangkan pada otot terlihat diameter miofibril ratio 1,28:1. Pada kelompok C dan D terlihat pelebaran arteri hepatika, duktus biliaris, dan vena porta yang berisi fibrin darah, serta sel-sel radang di sekitar segitiga Kiernan. Pada kelompok C diameter miofibril ratio 1,43;1 dan pada kelompok D 2,14:1. Pada kelompok E, F dan G terdapat sel-sel perlemakan mikrovesikuler di perifer sedangkan pada otot diameter miofibril ratio kelompok E 1,43:1, kelompok F 2,1:1, dan kelompok G 2,28:1. Simpulan: Pada pemberian AAS injeksi dosis rendah dan dosis tinggi kurang dari 4 minggu terjadi peradangan hati, pelebaran vena porta, arteri hepatika dan duktus biliaris sedangkan lebih dari 4 minggu terdapat perlemakan (steatosis) fokal hati. Pemberian AAS injeksi dosis rendah dan tinggi dalam waktu 4,6 dan 8 minggu menunjukkan pembesaran otot rangka (hipertrofi otot).Kata kunci: AAS, hati, otot rangka

scholarly journals The effects of buccal administration of azaperone on the sedation level and biochemical variables of weaned piglets

2021 ◽  
Vol 90 (1) ◽  
pp. 47-56
Author(s):  
Martin Svoboda ◽  
Zdeněk Fajt ◽  
Michaela Mruvčinská ◽  
Jan Vašek ◽  
Jana Blahová
Keyword(s):  
Body Weight ◽  
Blood Plasma ◽  
Animal Health ◽  
Weaned Piglets ◽  
Sedation Level ◽  
Biochemical Variables ◽  
Group A ◽  
Group B ◽  
Double Blinded ◽  
Group D

The aim of the study was to compare the efficacy of buccal and parenteral administration of azaperone in order to achieve pig sedation. The type of study was prospective randomized and double blinded. A total of 40 weaned piglets were divided into 4 groups (10 each) and monitored. Group A was injected intramuscularly (i.m.) with azaperone (Stresnil®, 40 mg/ml inj., Elanco Animal Health) at a dose of 2 mg/kg body weight (b.w.). Group B (control) was given 1 ml of saline buccally. Group C received a dose of 2 mg/kg b.w. of azaperone buccally. Group D was given azaperone buccally at a dose of 4 mg/kg b.w. The response to defined stimulus (a blunt blow of a metal rod into the metal edge of the pen), degree of salivation, movement level, blood plasma azaperone concentration, and the haematological and biochemical variables were included in the study. We found that the buccal administration of azaperone is effective, however, a dose of 4 mg/kg b.w. is required to induce a sedation level comparable to the standard 2 mg/kg b.w. i.m. administration.

scholarly journals In Vivo Study on Site of Action of Sinapine Thiocyanate following Acupoint Herbal Patching

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Shan Chen ◽  
Yu-Tong Jin ◽  
Zheng-Yang Zhu ◽  
Ling-Tao Wu ◽  
Ping Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Wistar Rats ◽  
Pharmacokinetic Profile ◽  
Time Intervals ◽  
Site Of Action ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Group D

Objective. To investigate the site of action of sinapine thiocyanate (ST), following acupoint herbal patching (AHP). Methods. Twenty Wistar rats were randomized into five groups (groups A, B, C, D, and E), and all groups received the same AHP in vivo. Skin samples were excised at 2 h, 4 h, 6 h, 10 h, and 26 h after AHP administration from group A to group E separately and the concentrations of ST in the skin were determined using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A pharmacokinetic profile of ST following AHP was performed at the same time in a group of five Wistar rats to detect plasma levels at the same time intervals. Results. The mean ± SD ST concentrations (ng/ml) at 2 h (group A), 4 h (group B), 6 h (group C), 10 h (group D), and 26 h (group E) after AHP administration were 250.01±61.99, 61.01±30.41, 40.12±26.94, 78.66±59.43, and 19.55±18.95, respectively. No ST was detected in rats’ plasma samples at the same time points. Conclusions. The site of action of ST following AHP is in the skin.

scholarly journals Effects of selected vitamins and minerals on growth rate and hematological parameters in broilers

2016 ◽  
Vol 1 (3) ◽  
pp. 487-494 ◽  
Author(s):  
Mohammad Harun Ar Rashid ◽  
Nazim Ahmad ◽  
Mohammad Rohul Amin ◽  
Mohammad Lalmoddin Mollah
Keyword(s):  
Body Weight ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Hematological Parameters ◽  
Haemoglobin Concentration ◽  
Erythrocyte Sedimentation ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Standard Ration

The present study was designed with a view to study the body weight and hematological parameters; total erythrocyte count, haemoglobin concentration, packed cell volume and erythrocyte sedimentation rate of growing broilers fed with standard poultry ration. Broilers were kept under close observation for a period of 14 days and body weight was measured at each 7 days interval up to the end of the 35 days of experimental period. A total of 20 (7 days old) broiler chicks were reared throughout the entire period of study. These chicks were randomly divided into four equal groups (n=5). Group A considered as control, fed only standard ration. Group B, C and D was considered as treated group. Group B with vitamin AD3E 1 ml/5 liter drinking water. Group C treated with mineral 1 ml/5 liter dirking water and Group D treated with vitamin (AD3E) 1 ml/5liter and mineral 1 ml/5 liter dirking water with standard ration. It was observed that body weight of broiler increased significantly (p<0.01) in group D compare to group A, B, & C. Total erythrocyte count, haemoglobin concentration and erythrocyte sedimentation rate values were significant (p<0.01) in the treated groups as compared to that of control group A. So it is concluded that vitamin (AD3E) and mineral supplementation with standard ration is beneficial for broiler production and economically profitable.Asian J. Med. Biol. Res. December 2015, 1(3): 487-494

Effects of Ganoderma Lucidum and Krestin on Subset T-cell in Spleen of γ-irradiated Mice

1995 ◽  
Vol 23 (03n04) ◽  
pp. 289-298 ◽  
Author(s):  
Wang-Chi Chen ◽  
Dou-Mong Hau ◽  
Chih-Chung Wang ◽  
I-Hsin Lin ◽  
Shiuh-Sheng Lee
Keyword(s):  
Body Weight ◽  
Ganoderma Lucidum ◽  
Radiation Treatment ◽  
Whole Body ◽  
Control Group ◽  
Γ Irradiation ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Better Than

Effects of Ganoderma lucidum (Gl) and Krestin (PSK) extracts on spleen, thymus and splenocytes in γ-irradiated mice were investigated in this study. ICR strain male mice were divided into five groups. Group A was the normal control. Group B, the experimental control, was treated with Gl. Group C, the radiation treatment control, was treated with whole body exposure to 4 Gy γ-irradiation (RT). Group D was treated with RT and Gl. Group E was treated with RT and PSK. The dosage of Gl was 400 mg/day/kg body weight and PSK was 500 mg/day/kg body weight. Our results indicated that the relative thymus weight in groups D and E were higher than group C on day 28 after γ-irradiation. Group D was the highest in all the experimental groups. CD4 and CD8 splenocytes in group D were higher than group C on days 7 and 28. Gl was better than PSK in repairing the damage of subset T-cells in the spleen of γ-irradiated mice.

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