scholarly journals Изменение функциональной активности тромбоцитов при длительном световом воздействии в эксперименте

2021 ◽  
Vol 129 (6) ◽  
pp. 711
Author(s):  
О.В. Злобина ◽  
В.Ф. Киричук ◽  
С.С. Пахомий ◽  
А.Н. Иванов ◽  
А.Ю. Каретникова ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Light Exposure ◽  
White Male ◽  
The Body ◽  
Male Rats ◽  
Light Transmittance ◽  
Laboratory Animals ◽  
Transmittance Curve ◽  
Microcirculatory Disturbances ◽  
Aggregation Activity

We studied the effect of intensive light exposure on the aggregation activity of white male rats' platelets using the experimental Light-Dark (18:6) lighting model. Analysis of platelet aggregation activity was carried out using a computerized 230LA "Biola" aggregation analyzer using the method of V.A. Gabbasov. It was established that in case of prolonged light exposure in the body of laboratory animals there was a disturbance of aggregation activity accompanied by an increase in the parameters of the curve of the average weighted radius of platelets and the parameters of the light transmittance curve. Violation of the daytime regime in the form of artificial prolongation of illumination causes increase of platelet aggregation activity and provokes development of microcirculatory disturbances. Forming changes in the hemostasis system increase in proportion to the duration of the experiment. The most pronounced changes in the vascular-platelet mechanism of hemostasis are formed on the 21st day of the experiment, indicating the development of processes of maladaptation followed by exhaustion of the organism.

scholarly journals Assessment of changes in the main homeostasis indicators in mammals after hyperthermic exposure

2021 ◽  
Vol 100 (12) ◽  
pp. 1397-1403
Author(s):  
Vladimir E. Kriyt ◽  
Yuliya N. Sladkova ◽  
Maksim V. Sannikov ◽  
Aleksandr O. Pyatibrat
Keyword(s):  
White Male ◽  
The Body ◽  
Male Rats ◽  
Laboratory Animals ◽  
Physical Factors ◽  
Electrolyte Balance ◽  
Leading Indicators ◽  
Acid Base ◽  
Blood Tests ◽  
The Impact

Introduction. The occupation of firefighters is characterized by the impact of both hazardous fire factors and a complex of harmful and dangerous characteristics of various natures, often exceeding the expected values. High air temperature is typical for almost all types of fire and is one of the main adverse physical factors affecting the body of firefighters. Experimental models on animals are of leading importance in studying extreme fire factors, including hyperthermic exposure. Material and methods. The study was carried out on 124 outbred white male rats of 3 months of age weighing 250-300 g, divided randomly into two groups. The first group (62 rats) was subjected to a single hyperthermic load. The second group (62 rats) was subjected to daily hyperthermic load during 14 days. The study of the leading indicators of the acid-base state and water-electrolyte balance, biochemical and clinical blood tests, cellular and humoral immunity was carried out before and after hyperthermic exposure. Results. The obtained data indicate high ambient temperatures cause changes in homeostasis indices in laboratory animals. At the same time, there are alterations in the indicators of water-electrolyte balance, acid-base state, clinical and biochemical blood tests and the immune system. Conclusion. An experimental model of extreme heat exposure on animals showed the emergence and persistence of changes in the leading indicators of homeostasis. In the group of single hyperthermia, these parameters were restored to the background levels in a day. In the group of multiple hyperthermia, they remained altered.

scholarly journals PECULIARITIES OF BILIARY FUNCTION OF THE LIVER IN THE DYNAMICS OF POLYTRAUMA IN THE EXPERIMENT

2020 ◽  
Vol 16 (3) ◽  
pp. 9-13
Author(s):  
D.V. Kozak
Keyword(s):  
General Pattern ◽  
White Male ◽  
Bile Secretion ◽  
The Body ◽  
Digital Data ◽  
Male Rats ◽  
Subsequent Decrease ◽  
Biliary Function ◽  
Thiopental Sodium ◽  
Main Components

Relevance. Severe trauma is accompanied by the development of multiple organ dysfunction and the insufficiency of internal organs. The dynamics of changes in the functional state of the liver didn't investigate fully. It is the central organ of detoxification of the body, whose activity occurs in close integration with other organs and systems of the body. The objective is to find out the features of the biliary function of the liver in the dynamics of the developed model of polytrauma. Materials and methods. Polytrauma was performed using 62 nonlinear white male rats weighing 180-200 g under conditions of thiopental-sodium anesthesia (40 mg 1kg-1 intraperitoneally). In surviving animals, the biliary function of the liver was studied in 2 h, 1, 3, 7, 14, 21, and 28 days after injury. For this purpose, the common bile duct was catheterized, and bile was collected for 1 hour in animals under thiopental-sodium anesthesia (60 mg kg-1). The rate of bile excretion and the concentration of total bile acids, cholesterol, direct and indirect bilirubin in the selected portion of bile were determined. Based on these data, the rate of excretion of the studied components of bile was calculated. Euthanasia of rats throughout the experiment was performed by total bloodletting from the heart after previous thiopental-sodium anesthesia (60 mg kg-1 intraperitoneally). The obtained digital data were subject to statistical analysis. Results. In the conditions of experimental polytrauma, there is a violation of the biliary function of the liver. It is manifested in the period of an acute reaction to the trauma first (after 2 hours) by a significant it decreases, then (up to 1 day) development of polycholia - 1.52 times increased of bile secretion. Also increased excretion of the main components of bile, with their subsequent decrease to 7 days, development of the period of temporary improvement in 14 days with the repeated of exacerbation period in 21 days and approach to the norm - in 28 days. The decrease in bile secretion corresponds to a period of shock, which is characterized by the centralization of blood circulation and reduced blood supply to the organs of the gastrointestinal tract. Increased bile secretion and excretion of main bile components in 1 day after a severe injury is associated with the increased biliary polarity of hepatocytes and unloading of the liver from endotoxins. It accumulates due to tissue damage, microcirculation, and hypoxia. Subsequently, the indicators of the biliary function of the liver changed by the identified patterns of lipoperoxidation deviations, antioxidant protection, cytolysis, and endogenous intoxication. The pathogenesis of biliary disorders is the damage of the endoplasmic reticulum membranes, where the synthesis of the main components of bile. As well as the development of edema of the organ, which prevents the outflow of bile. Conclusion. The dynamics of the development of functional liver failure due to polytrauma coincides with the general pattern characteristic of the dynamics of other biochemical markers of traumatic disease. Namely: after 3 days of the post-traumatic period, there was a phase of maximum deepening of deviations of the studied indexes. After 7-14 days there was noted a phase of temporary improvement which is characterized by a change of indexes towards the norm. After 21 days there was a re-exacerbation of the pathological process. After 28 days the indexes changed towards the norm, but for most cases do not reach it. This means that in a critical state of the body the organs and systems coupling is getting worse, which are remote from the site of injury. It can be considered as a factor of compensation and adaptation directed to the survival of the organism.

scholarly journals Features of morphological changes in the thyroid gland of white male rats 1 day after thermal trauma of the skin on the background of the introduction of 0.9 % NaCl solution

2019 ◽  
pp. 55-59
Author(s):  
O. I. Tiron
Keyword(s):  
Thyroid Gland ◽  
White Male ◽  
Experimental Studies ◽  
Vena Cava ◽  
Scientific Data ◽  
The Body ◽  
Male Rats ◽  
Nacl Solution ◽  
Experimental Animals ◽  
Thermal Trauma

Despite the fact that the main pathogenetic factor in the development of burn disease is the alteration of the skin, the severity of the condition of patients in the acute period of the disease is due to systemic changes in the body caused by thermal factor. Being a powerful stress factor, severe burns are accompanied by activation of the hypothalamic-pituitary system. Although thyroid hormones are not considered typical "stress hormones", they also play a role in maintaining vasomotor function under stress. The question of thyroid dysfunction on the background of burn injury remains debatable, and scientific data on its morphological and ultramicroscopic changes are quite limited. The aim of the work is to establish microscopic and submicroscopic changes of the thyroid gland of experimental animals in the stage of shock after thermal trauma of the skin. Experimental studies were performed on 12 white adult male rats, which received a thermal burn of the skin of 2-3 degrees with a lesion area of 21-23 % of the skin. An infusion of 0.9 % NaCl solution at a dose of 10 ml/kg was performed into the inferior vena cava. Collection of material for histological and electron microscopic examinations was performed according to generally accepted methods. Histological specimens were examined using a MIKROmed SEO SCAN light microscope and photo-documented using a Vision CCD Camera with an image output system from histological specimens. Ultrathin sections made on an ultramicrotome UMPT-7 were contrasted with uranyl acetate, lead citrate according to the Reynolds method and studied under an electron microscope PEM-125K. Conducted micro- and submicroscopic studies of the structural components of the thyroid gland a day after the reproduction of thermal trauma to the skin on the background of the introduction of 0.9 % NaCl solution revealed reactive adjunctive-compensatory changes and initial manifestations of destruction. Vascular, stromal and parenchymal components of the organ in the stage of shock after burns correspond to the state of "stress" as a reaction to pathological exogenous exposure, and the intensification of metabolic processes in the affected organism of experimental animals.

scholarly journals Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

2020 ◽  
Vol 3 (2) ◽  
pp. 38
Author(s):  
Ayu Mayang A. P. ◽  
Liliawanti Liliawanti ◽  
Wachjudi Kurnia
Keyword(s):  
Rattus Norvegicus ◽  
White Male ◽  
Brown Seaweed ◽  
Negative Control ◽  
Laboratory Method ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference

<p>Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (<em>Sargassum duplicatum</em>) which can increase hematocrit levels in the blood of white male wistar strain rat (<em>Rattus norvegicus</em>) suffered from anemia in induction NaNO<sub>2</sub>.</p><p>            This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K<sub>0</sub>) without treatment, the positive control (K<sub>1</sub>) be treated anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract and treatment group (K<sub>2</sub>) treated with anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract at a dose of 2,45gr / kgBB. On the 40<sup>th</sup> day, a blood sample was taken, then measured the hematocrit level.</p><p>            The results showed that the mean number of blood hematocrit levels in K<sub>2</sub> was higher than K<sub>1</sub>, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p &gt; 0,05 showed that there were no statistically significant differences between groups of experimental animals.</p><p>            From the results of this research, the conclusion is the administration of brown seaweed extract (<em>Sargassum duplicatum</em>) descriptively increased, but not statistically significant.</p><p> </p><p><strong>Keywords :</strong> Anemia, hematocrit, <em>Sargassum duplicatum</em>, NaNO<sub>2</sub></p>

scholarly journals Acute Oral Toxicity of the Supramolecular Complex Based on Albendazole and Triclabendazole (Altric-Extra) in Laboratory Outbred Mice and Rats

2020 ◽  
Vol 14 (1) ◽  
pp. 64-69
Author(s):  
Ekaterina V. Lagereva ◽  
Vladislav E. Abramov
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
The Body ◽  
Male Rats ◽  
Laboratory Animals ◽  
Oral Toxicity ◽  
Hazard Class ◽  
Russian Research Institute ◽  
Starch Gel ◽  
Outbred Mice

The purpose of the research is to evaluate the acute toxicity of Altric-Extra when introduced into the stomach to mice and rats. Materials and methods. The studies were conducted in the vivarium of the All-Russian Research Institute of Fundamental and Applied Parasitology of Animals and Plants. The acute toxicity of Altric-Extra was determined on 20 white outbred male mice weighing 19.3–23.3 g, 10 animals in a group and on 30 white outbred male rats weighing 150–196 g, 6 animals in a group. Altric-Extra was administered to mice of the experimental group once into the stomach in the form of a suspension in a dose of 5,986 mg/kg at the rate of 0.2 ml/10 g of body weight. Altric-Extra rats were also administered once into the stomach in the form of a suspension at the rate of 2.0 ml/100 g body weight. As a carrier in the preparation of the suspension, 1% starch gel was used. The experimental rats of groups 1, 2, 3 and 4 were given Altric-Extra at doses of 4,580.2 mg/kg, 3,846.2; 3,088.8 and 1,577.9 mg/ kg respectively. Mice and rats of the control groups were administered once with 1% starch gel. For 14 days, the behavior and condition of the animals was monitored. The body weight of the experimental animals was measured on the 1st, 3rd, 7th, 9th and 14th days of the experiment. Results and discussion. Medium lethal doses of LD50 have been established for oral administration to laboratory animals. For mice, the LD50 was more than 5 986 mg/kg, i.e., according to the generally accepted hygienic classification, Altrick-Extra belongs to hazard class 4 (low-hazard substances). On rats, the LD50 was 3 103.1±48.5 mg/kg (2,354.6÷3,851.5 mg/kg). Therefore, Altrik-Extra belongs to hazard class 3 (substances are moderately hazardous).

STUDY OF THE LOCAL ANTIOXIDANT SYSTEM IN RATS AFTER TOOTH EXTRACTION IN A COMPARATIVE EXPERIMENT

2020 ◽  
pp. 65-68
Author(s):  
Арпине Бронислави Антонян ◽  
Дмитрий Юрьевич Харитонов ◽  
Алик Эдикович Петросян ◽  
Эльвира Валерьевна Ветрова
Keyword(s):  
Tooth Extraction ◽  
White Male ◽  
Practical Importance ◽  
Daily Practice ◽  
Mucosal Healing ◽  
Oxidative Status ◽  
The Body ◽  
Male Rats ◽  
Laboratory Assessment ◽  
Hydrogen Water

Проблема недостаточной прогнозируемости развития осложнений после операции удаления зуба и заживления слизистой оболочки имеет актуальность в ежедневной практике врача стоматолога-хирурга. Использование белых крыс-самцов линии Вистар в качестве биомоделирования сложного удаления зуба позволяет провести визуальную и лабораторную оценку течения раневого процесса и оценить влияние растворов, ионизированных серебром, и водородной воды на местный оксидативный статус. Исследование прооксидантного и антиоксидантного состояния имеет важное практическое значение, поскольку позволяет оценить местные компенсаторные возможности организма. Состояние равновесия характерно для физиологической нормы, в то время как воспалительные процессы приводят к разности между этими системами. Статья посвящена осложнениям, возникающим в области удаленных зубов у лабораторных крыс. Изучали влияние физиологического раствора, ионизированного серебром, и водородную воду в качестве жидкости для орошения операционного поля и влияние данных растворов на местный оксидативный статус, а также течение раневого процесса и эпителизацию лунки. Полученные в ходе экспериментального исследования результаты подтверждают необходимость применения новых растворов для орошения оперируемого поля в процессе операции удаления зуба The problem of insufficient prognosticability of complications after tooth extraction and mucosal healing is relevant in the daily practice of a dentist-surgeon. The use of white male rats of the Wistar line as a biomodeling of complex tooth extraction allows for visual and laboratory assessment of the course of the wound process and to assess the effect of solutions ionized with silver and hydrogen water on the local oxidative status. The study of the Pro-oxidant and antioxidant state is of great practical importance, since it allows us to evaluate the local compensatory capabilities of the body. The state of equilibrium is characteristic of the physiological norm, while inflammatory processes lead to a difference between these systems. The article is devoted to complications that occur in the area of removed teeth in laboratory rats. We studied the effect of saline solution ionized with silver and hydrogen water as a liquid for irrigation of the operating field and the effect of these solutions on the local oxidative status, as well as the course of the wound process and epithelization of the well. The results obtained during the experimental study confirm the need to use new solutions for irrigation of the operated field during the operation of tooth extraction

scholarly journals PENGARUH PEMBERIAN EKSTRAK PEPAYA TERHADAP KADAR KOLESTEROL TOTAL, LDL DAN HDL DARAH TIKUS PUTIH JANTAN HIPERKOLESTEROLEMIA

2012 ◽  
Vol 36 (1) ◽  
pp. 29 ◽  
Author(s):  
Endrinaldi Endrinaldi ◽  
Asterina Asterina
Keyword(s):  
Total Cholesterol ◽  
Bile Acids ◽  
White Male ◽  
Serum Levels ◽  
The Body ◽  
Water Soluble ◽  
Male Rats ◽  
Intestinal Lumen ◽  
Control Group ◽  
Soluble Fiber

AbstrakPepaya mengandung pektin yang merupakan serat larut air. Pektin berkemampuan mengikat asam empedu dan dieliminasi keluar tubuh melalui feses. Penurunan jumlah asam empedu di lumen usus menyebabkan hepar menggunakan kolesterol dalam darah untuk membentuk asam empedu. Hal tersebut mengakibatkan penurunan jumlah kolesterol.Tujuan studi ini adalah untuk melihat pengaruh pemberian ekstrak pepaya mengkal terhadap kadar kolesterol total, HDL dan LDL tikus putih. Desain penelitian ini adalah eksperimental menggunakan 20 ekor tikus putih jantan yang dibagi menjadi empat kelompok, yaitu kelompok kontrol, dan kelompok perlakuan dengan pemberian ekstrak pepaya dengan dosis 0,5 ml, 1 ml dan 1,5 ml per 200g/BB melalui oral selama 15 hari.Hasil penelitian menunjukkan terjadinya penurunan rerata kadar kolesterol total, LDL, dan peningkatan kadar HDL serum tikus setelah pemberian ekstrak pepaya selama 15 hari secara bermakna (p<0,05). Tetapi tidak terjadi perbedaan yang bermakna (p>0,05) antara kelompok yang diberi ekstrak pepaya dengan dosis 0,5 ml, 1 ml, dan 1,5 ml.Kesimpulan yang dapat diambil dari studi ini ialah bahwa ekstrak pepaya dapat menurunkan kadar kolesterol total, LDL, dan peningkatan kadar HDL pada serum tikus putih jantanKata kunci : pektin, kolesterol, asam empedu, serat, tikus.AbstractPapaya contain pectin which is a water-soluble fiber. Pectin has the ablity to bind bile acids and eliminated out of the body through the feces. Decrease in the amount of bile acids in intestinal lumen causing liver uses cholesterol to synthesize bile acids blood.The purpose of this study was to see the effect of papaya extract on total cholesterol, HDL and LDL of male rats. This is an experimental research design using 20 white male rats were divided into four groups : control group and group treated with papaya extract a dose of 0,5 ml, 1 ml per 200 gr/body weight orally for 15 days.Results showed a decrease in everage total cholesterol, LDL and increased serum levels of rats after administration of papaya extract after 15 days wereARTIKEL PENELITIAN30significantly (p<0,05). But no significantly difference occused (p>0,05) between groups were fed papaya extractat a dose of 0,5 ml, 1 ml and 1,5 ml.Conclusions from the study are that papaya extract can lower total cholesterol, LDL and levels increased in the serum of male ratsKey word : pectin, cholesterol, bile acid, fiber, rats.

scholarly journals Submicroscopic changes of alveoli of respiratory department of lungs a day after experimental thermal trauma

2019 ◽  
Vol 25 (3) ◽  
pp. 16-20
Author(s):  
Z.M. Nebesna ◽  
O.I. Bashynska ◽  
N.P. Ocheretna ◽  
G.М. Galunko ◽  
O.Ya. Slyvka
Keyword(s):  
Burn Injury ◽  
White Male ◽  
Pathological Process ◽  
The Body ◽  
Male Rats ◽  
Structural Components ◽  
Thermal Lesion ◽  
Thermal Trauma ◽  
Impaired Lung Function ◽  
Ketamine Anesthesia

Deep, large thermal burns are not limited to local lesions of tissues, they cause significant disruption of all systems and organs of the organism, change in metabolic processes. It is revealed that the primary links in the pathogenesis of burn disease are destruction of the skin, impaired neuroendocrine regulation and significant hemodynamic disorders. The reorganization of structures and impaired lung function, in response to a pathological process in the body, is attracting increasing attention of scientists. The aim of the study was to establish a submicroscopic rearrangement of the alveoli after a thermal lesion for 1 day after the experimental thermal trauma. Grade III burns were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97-1000С. The size of the lesion area was 18-20 % of the epilated surface of the body of rats. An experimental study of the structural components of lung alveoli after burn injury was performed on laboratory white male rats weighing 160-180 g. Euthanasia of rats was performed after ketamine anesthesia by decapitation. In the experiment, the study of the submicroscopic state of the walls of the alveoli of the lungs after thermal trauma was done. It is established that in the stage of shock after the application of burn injury – 1 day, in the alveoli of the respiratory department of the lung, there are adaptive compensatory and initial destructive changes of all structural components of the alveoli. Damage to the ultrastructure of the aerohematical barrier is manifested by intracellular edema and edema of the organelles of the endothelial cells, respiratory and secretory epitheliocytes, and the amount of heterochromatin increases in their deformed nuclei. The basement membrane also has signs of edema, sometimes homogeneous, fuzzy. The decrease in the number of vesicles and micropinocytotic vesicles in endothelial and respiratory epitheliocytes leads to impaired endothelial and alveolar metabolism. Numerous actively phagocytic alveolar macrophages with a well-expressed lysosomal apparatus are found in the alveoli. Initial alternative alterations of the ultrastructure of the components of the air-barrier barrier lead to disruption of gas exchange in the respiratory department of the lungs

scholarly journals Modeling of the acute pyelonephritis in animals of various kind

2006 ◽  
Vol 5 (4) ◽  
pp. 42-47
Author(s):  
A. V. Lukjanov ◽  
V. T. Dolgikh ◽  
E. G. Potievsky ◽  
B. A. Rais ◽  
T. F. Sokolova ◽  
...  
Keyword(s):  
Acute Pyelonephritis ◽  
Asymptomatic Bacteriuria ◽  
The Body ◽  
Male Rats ◽  
Laboratory Animals ◽  
E Coli ◽  
Acute Infections ◽  
High Virulence ◽  
Universal Mechanism ◽  
Infectious Organism

Experiments have been carried out with two kinds of laboratory animals: 27 rabbits of chinchilla breed with the age of 3—4 months and the weight of the body from 1740 to 2 290 grams (1 993,1 ± 243,2) and 49 white alley male rats with the weight of the body of 180—220 g (195,4 ± 27,3). As an infectious organism, the daily dredge hemolysinogen E. coli with colicinogenic factor Ca 38 was used. Roots of introduction of dredge E. coli were intravenous, transurethral, transcapsular in parenchyma kidneys, enteral and endotracheal (in rats). As the animals were infected through various entrance gates, three variants of acute infections of the uric system were obtained: purulent-destructive defeats, tubular-interstitial infringements and asymptomatic bacteriuria. High virulence of the urostrain E. coli , used in the experiment, is demonstrated. The hypothesis about the universal mechanism of infection of the uric system "from top to down", i.e. hematogenic, has been formulated.

scholarly journals EXPERIMENTAL STUDY OF THE IMPACT OF LOW FLUORINE CONCENTRATIONS ON THE TISSUE LEVEL OF HSP FAMILY PROTEINS

2018 ◽  
Vol 97 (7) ◽  
pp. 604-608
Author(s):  
Anna G. Zhukova ◽  
L. G. Gorokhova ◽  
A. V. Kiseleva ◽  
T. G. Sazontova ◽  
N. N. Mikhailova
Keyword(s):  
Toxic Effect ◽  
White Male ◽  
Antioxidant Properties ◽  
Tissue Level ◽  
Male Rats ◽  
Laboratory Animals ◽  
Heme Oxygenase 1 ◽  
Sensitive Organ ◽  
The Impact ◽  
The Brain

Introduction. Fluoride in high concentrations has a toxic effect not only on bone tissue but also on the heart, liver, kidneys, and brain. In the implementation of the response to toxic doses of fluorine the proteins of the HSP family are involved regulating intracellular and tissue homeostasis under various stress effects. The toxic effect of high fluorine concentrations the mechanisms of which are disclosed in fluorosis can be realized and at a level significantly lower than a toxic one. In the literature, there is little data on the peculiarities of the effects of low fluorine concentrations at the tissue and cellular levels. The aim of the study. To investigate the impact of low fluorine concentrations on the tissue level of HSP family proteins in the brain and liver of laboratory animals. Material and methods. The experiments were carried out on 60 white male rats of the same age weighing 200-250 g. The rats were divided into 2 groups: the control and the group of the animals exposed to sodium fluoride (NaF) within 6 weeks (at a concentration of 10 mg/l corresponding to the daily fluorine dose of 1.2 mg/kg per body weight). We determined the level of inducible HSP72 and HSP32 (heme-oxygenase-1) referred to proteins of HSP family (Heat shock proteins), the activity of free radical processes and antioxidant enzymes (superoxide dismutase (SOD) and catalase) in the brain and liver tissues. Results. The important role of stress-inducible HSP72 protein in protecting the brain from the damage caused by the prolonged exposure to low fluorine concentrations was shown. In the liver, a protective role against fluoride exposure is played by the protein HSP32 with antioxidant properties. At the tissue level, the prolongation of the terms of the development of chronic fluoride intoxication with low fluorine concentrations was revealed. In the liver appeared to be the highly sensitive organ to the fluorine accumulation, the significant lesion was detected.

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