scholarly journals Biomarkers of the type of recurrent uterine myoma-associated growth

2021 ◽  
Vol 12 (4) ◽  
pp. 6-11
Author(s):  
E. V. Bashirov ◽  
V. A. Krutova ◽  
I. I. Kutsenko
Keyword(s):  
Hormone Receptors ◽  
Doppler Sonography ◽  
Inflammatory Diseases ◽  
Treatment Options ◽  
Blood Perfusion ◽  
Tissue Growth ◽  
Uterine Myoma ◽  
Steroid Hormone Receptors ◽  
Reproductive Age ◽  
Proliferative Potential

Objective: To assess the diagnostic value of biomarkers: microbiological, molecular and biological, immunological biomarkers, characteristic of various types of recurrent myoma-associated growth.Materials and Methods: Seventy women of reproductive age with recurrent uterine myoma and its combination with adenomyosis after conservative treatment in the Clinic of Kuban State Medical University were examined. Methods: microbiological examination, sonography, Doppler sonography, histology, immunohistochemistry, morphometry.Results: The type of recurrent myoma-associated growth was proved to be dependent on molecular and biological characteristics of tumors, the presence of infection and blood perfusion. It was indicated that women with recurrence of myoma-associated growth of a “false” type were characterized by high rates of infections (the presence of reproductive losses, chronic inflammatory diseases of the pelvic organs) and significant bacterial contamination of the genital tract biotopes. Blood perfusion features were identified for true and “false” types of recurrent myoma-associated growth based on Doppler sonography data, which were consistent with features of tumor vessel morphometry. Analysis of uterine myoma histological types and their vascularization features showed correlation of forms with a high proliferative potential of a tumor on a molecular and cellular level to moderate and high expression of steroid hormone receptors in combination with Ki67, a significant diameter of the lumen of the vessels with the highest VI and VFI values.Conclusions: A comprehensive study of women with uterine hyperplasia determines the possibility of prediction of pathogenetic variants of recurrent myoma-associated tissue growth and adequate choice of treatment options and rehabilitation course.

scholarly journals Sex steroids regulate skin pigmentation through nonclassical membrane-bound receptors

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Christopher A Natale ◽  
Elizabeth K Duperret ◽  
Junqian Zhang ◽  
Rochelle Sadeghi ◽  
Ankit Dahal ◽  
...  
Keyword(s):  
Hormone Receptors ◽  
Treatment Options ◽  
Skin Pigmentation ◽  
Progesterone Receptors ◽  
Steroid Hormone Receptors ◽  
Pigment Synthesis ◽  
17Β Estradiol ◽  
Membrane Bound ◽  
Epidermal Melanocyte ◽  
G Protein Coupled

The association between pregnancy and altered cutaneous pigmentation has been documented for over two millennia, suggesting that sex hormones play a role in regulating epidermal melanocyte (MC) homeostasis. Here we show that physiologic estrogen (17β-estradiol) and progesterone reciprocally regulate melanin synthesis. This is intriguing given that we also show that normal primary human MCs lack classical estrogen or progesterone receptors (ER or PR). Utilizing both genetic and pharmacologic approaches, we establish that sex steroid effects on human pigment synthesis are mediated by the membrane-bound, steroid hormone receptors G protein-coupled estrogen receptor (GPER), and progestin and adipoQ receptor 7 (PAQR7). Activity of these receptors was activated or inhibited by synthetic estrogen or progesterone analogs that do not bind to ER or PR. As safe and effective treatment options for skin pigmentation disorders are limited, these specific GPER and PAQR7 ligands may represent a novel class of therapeutics.

scholarly journals Ontogeny and expression profiles of steroid hormone receptors in a mouse model of endometriosis

2019 ◽  
Author(s):  
Anuradha Mishra ◽  
Mosami Galvankar ◽  
Neha Singh ◽  
Shantashri Vaidya ◽  
Uddhav Chaudhari ◽  
...  
Keyword(s):  
Mouse Model ◽  
Stromal Cells ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Expression Profiles ◽  
Steroid Hormone Receptors ◽  
Reproductive Age ◽  
Unknown Etiology ◽  
Mrna Levels ◽  
Post Surgery

ABSTRACTEndometriosis is a chronic incurable disorder of unknown etiology affecting a large proportion of women in reproductive age. In order to understand the pathogenesis and preclinical testing of drugs,animal models that recapitulate the key features of the disorder are highly desirous. Herein, we describe the ontogeny of the ectopic endometrial lesion in a mouse model where uterine tissue was ligated to the intestinal mesentery and the animals were followed up from day 5 to day 60 post-surgery. Out of 60 animals that underwent surgery, 58 developed endometriosis using this strategy. Most lesions were pale, fluid filled while red lesions were seen in ~10% of animals. Histologically, in most animals there was one large cystic gland with well differentiated epithelium, in 13% of animals there was mixed phenotype (well and poorly differentiated). There was extensive stromal compaction and increased number of macrophages in ectopic lesions. During the course of endometriosis, there was an increase in number of PCNA positive epithelial and stromal cells. The epithelial cells at all the time point were cytokeratin positive and the stroma was vimentin positive. However, at day 30 and 60, the stromal cells were also cytokeratin positive. The mRNA levels of estrogen receptorsEsr1andGper1were reduced while those ofEsr2were elevated as compared to normal endometrium, the levels of progesterone receptor (Pgr) were found to be downregulated in ectopic lesions as compared to control. However, these differences were not statistically significant due to high biological variability. Low abundance ofCyp19a1transcripts (aromatase gene) were only detected in the ectopic endometrium. Immunohistochemically, the expression of ERα and ERβ was significantly reduced only in stromal cells; the epithelial cell staining was maintained. GPER1 and PR immunoreactivity was significantly low in both epithelial and stromal cells. The immunostaining of all the steroid receptors was highly heterogeneous in the ectopic tissues with some areas of sections had stained intensely while others had negligible staining. We propose that temporal and spatial difference in the expression of steroid hormone receptors during the course of endometriosis development coupled with micro-heterogeneity may alter the effectiveness of steroid hormone analogues resulting in variable outcomes and often failure of therapy.

scholarly journals Deficiency of ERβ and prostate tumorigenesis in FGF8b transgenic mice

2014 ◽  
Vol 21 (4) ◽  
pp. 677-690 ◽  
Author(s):  
Teresa Elo ◽  
Lan Yu ◽  
Eeva Valve ◽  
Sari Mäkelä ◽  
Pirkko Härkönen
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Hormone Receptors ◽  
Intraepithelial Neoplasia ◽  
Estrogen Receptor Α ◽  
Tissue Growth ◽  
Steroid Hormone Receptors ◽  
Fibroblast Growth ◽  
Fibroblast Growth Factor Receptors ◽  
Prostate Tumorigenesis

Estrogens contribute to the development and growth of the prostate and are implicated in prostate tumorigenesis. In their target tissues, estrogens mediate their effects via estrogen receptor α (ERα (ESR1)) and β (ERβ (ESR2)). Hyperplasia and decreased differentiation of epithelial cells in the prostate have been reported in ERβ knockout (BERKO) mice. Herein, we studied the effect of ERβ deficiency on prostate tumorigenesis by crossing BERKOFVB mice with prostate-targeted human fibroblast growth factor 8b transgenic (FGF8b-Tg) mice. Consistent with results described in our previous report, the prostates of 1-year-old FGF8b-Tg mice displayed stromal aberrations, prostatic intraepithelial neoplasia (mPIN) lesions, inflammation, and occasionally cancer. The prostates of BERKOFVB mice exhibited mild epithelial hypercellularity and inflammation. The prostate phenotypes of FGF8b-Tg-BERKOFVB mice closely resembled those of FGF8b-Tg mice. However, mucinous metaplasia, indicated by Goblet-like cells in the epithelium, was significantly more frequent in the prostates of FGF8b-Tg-BERKOFVB mice when compared with FGF8b-Tg mice. Furthermore, compared with FGF8b-Tg mice, there was a tendency for increased frequency of inflammation but milder hyperplasias in the prostate stroma of FGF8b-Tg-BERKOFVB mice. The expression levels of mRNAs for FGF8b-regulated genes including osteopontin (Spp1), connective tissue growth factor (Ctgf), fibroblast growth factor receptors (Fgfrs), and steroid hormone receptors and cytokines were similar in the prostates of FGF8b-Tg and FGF8b-Tg-BERKOFVB mice. Our results indicate that ERβ plays a role in the differentiation of the prostatic epithelium and, potentially, in the defensive mechanism required for protection against inflammation but do not support a direct tumor-suppressive function of ERβ in the prostate of FGF8b-Tg mice.

scholarly journals Study of causal factors of uterine fibroids development in patients of reproductive age

2019 ◽  
Vol 2 (14) ◽  
pp. 52-54
Author(s):  
S. M. Safarova ◽  
G. U. Bolieva ◽  
N. J. Abdurahmanova
Keyword(s):  
High Frequency ◽  
Inflammatory Diseases ◽  
Varicose Veins ◽  
Uterine Fibroids ◽  
Mammary Glands ◽  
Uterine Myoma ◽  
Reproductive Age ◽  
Deficiency Anemia ◽  
Menstrual Dysfunction ◽  
Status Of Women

The somatic and gynecological status of women with uterine myoma is presented. A significantly high frequency of concomitant extragenital pathology was revealed, including: iron deficiency anemia, thyroid and urinary tract diseases, pathology of the gastrointestinal tract, obesity and varicose veins. It has been established that patients with uterine myoma often suffer from menstrual dysfunction of the type of hypermenstrual syndrome and algodimenorrhea, menometrorrhagia. More than half of the examined women with uterine myoma detected a combination of this pathology with endometrial hyperplasia, benign ovarian formations, pelvic inflammatory diseases, cystic fibrosis of the mammary glands.

Structure-Based Virtual Screening of Perfluoroalkyl and Polyfluoroalkyl Substances (PFASs) as Endocrine Disruptors of Androgen Receptor Activity Using Molecular Docking and Machine Learning

2020 ◽  
Author(s):  
Azhagiya Singam Ettayapuram Ramaprasad ◽  
Phum Tachachartvanich ◽  
Denis Fourches ◽  
Anatoly Soshilov ◽  
Jennifer C.Y. Hsieh ◽  
...  
Keyword(s):  
Machine Learning ◽  
Molecular Docking ◽  
Androgen Receptor ◽  
Endocrine Disruptors ◽  
Hormone Receptors ◽  
Steroid Hormone Receptors ◽  
Machine Learning Techniques ◽  
Support Vector ◽  
Polyfluoroalkyl Substances ◽  
Perfluoroalkyl And Polyfluoroalkyl Substances

Perfluoroalkyl and Polyfluoroalkyl Substances (PFASs) pose a substantial threat as endocrine disruptors, and thus early identification of those that may interact with steroid hormone receptors, such as the androgen receptor (AR), is critical. In this study we screened 5,206 PFASs from the CompTox database against the different binding sites on the AR using both molecular docking and machine learning techniques. We developed support vector machine models trained on Tox21 data to classify the active and inactive PFASs for AR using different chemical fingerprints as features. The maximum accuracy was 95.01% and Matthew’s correlation coefficient (MCC) was 0.76 respectively, based on MACCS fingerprints (MACCSFP). The combination of docking-based screening and machine learning models identified 29 PFASs that have strong potential for activity against the AR and should be considered priority chemicals for biological toxicity testing.

scholarly journals Chronic Inflammation in PCOS: The Potential Benefits of Specialized Pro-Resolving Lipid Mediators (SPMs) in the Improvement of the Resolutive Response

2020 ◽  
Vol 22 (1) ◽  
pp. 384
Author(s):  
Pedro-Antonio Regidor ◽  
Anna Mueller ◽  
Manuela Sailer ◽  
Fernando Gonzalez Santos ◽  
Jose Miguel Rizo ◽  
...  
Keyword(s):  
Chronic Inflammation ◽  
Unsaturated Fatty Acids ◽  
Inflammatory Diseases ◽  
Reproductive Age ◽  
Lipid Mediator ◽  
Female Population ◽  
Tnf Α ◽  
Potential Benefits ◽  
Cardinal Symptoms ◽  
Resolution Processes

PCOS as the most common endocrine disorder of women in their reproductive age affects between 5–15% of the female population. Apart from its cardinal symptoms, like irregular and anovulatory cycles, hyperandrogenemia and a typical ultrasound feature of the ovary, obesity, and insulin resistance are often associated with the disease. Furthermore, PCOS represents a status of chronic inflammation with permanently elevated levels of inflammatory markers including IL-6 and IL-18, TNF-α, and CRP. Inflammation, as discovered only recently, consists of two processes occurring concomitantly: active initiation, involving “classical” mediators including prostaglandins and leukotrienes, and active resolution processes based on the action of so-called specialized pro-resolving mediators (SPMs). These novel lipid mediator molecules derive from the essential ω3-poly-unsaturated fatty acids (PUFAs) DHA and EPA and are synthesized via specific intermediates. The role and benefits of SPMs in chronic inflammatory diseases like obesity, atherosclerosis, and Diabetes mellitus has become a subject of intense research during the last years and since PCOS features several of these pathologies, this review aims at summarizing potential roles of SPMs in this disease and their putative use as novel therapeutics.

Sign in / Sign up

Export Citation Format

Share Document