In vitro biodegradation of wheat straw and wheat straw based total mixed ration using zoospores of different elite ruminal fungi.

2018 ◽  
Vol 5 (04) ◽  
Author(s):  
SANJAY KUMAR ◽  
JP SEHGAL ◽  
AK PUNIYA ◽  
RAJNI KUMARI
Keyword(s):  
Wheat Straw ◽  
Stress Conditions ◽  
In Vitro Digestibility ◽  
Complex Media ◽  
Total Mixed Ration ◽  
Neutral Detergent Fibre ◽  
Acid Detergent Fibre

The elite anaerobic fungal cultures Neocallimastix sp GR-1, Piromyces sp WNG-12 andOrpinomyces sp C-14 were isolated from wild blue bull and cattle respectively of grazing and browsing goat. Attempts were made to grow thesecultures in normal media or using stress conditions by keeping them at lower or highertemperature than39oc or using a complex media; Normal media produced maximum number of fungal zoospores after 120hrs of incubation. The in- vitrodry matter, acid detergent fibre, neutral detergent fibre digestibilitywere studied using fresh zoospores; or those being kept at normal; lower orhigher temperature than 39oC. Based on in vitro digestibility of DM, NDF and ADF of wheat straw and wheat straw based totalmixed rations.The Neocallimastix sp GR-1was found better as compared to Piromyces sp WNG-12 andOrpinomyces sp C-14as this anaerobic fungiproduced maximum numbers of zoospores and also proved to be best performing for its abilityto degrade lignified feed material.With the addition of zoospores of Neocallimastix GR1 kept at different temperature for 45 days in treatments T2, T3, T4 and T5, NDF digestibility increased significantly (P≤0.05) and values were recorded as 42.54±0.54, 41.52±0.58, 41.00±0.56 and 42.27±0.55.respectively.With addition of zoospores of Piromyces sp.WNG-12, the NDF digestibility also increased significantly (P≤0.01) as compared to control both for WS and WS based rations.Addition of zoospores of Neocallimastix GR-1 to different treatments increased total number of zoospores significantly in all treatments compared to that of control.

Washing and filtration of wheat straw treated with sodium hydroxide alone or with hydrogen peroxide to modify cell wall composition and in vitro digestibility

1997 ◽  
Vol 37 (6) ◽  
pp. 617 ◽  
Author(s):  
A. S. Chaudhry
Keyword(s):  
Hydrogen Peroxide ◽  
Wheat Straw ◽  
Dry Matter ◽  
Ph Control ◽  
Fibre Content ◽  
In Vitro Digestibility ◽  
Neutral Detergent Fibre ◽  
Chemical Treatments ◽  
Dry Matter Digestibility

Summary. This study compared the effect of different levels (24–160 g/kg DM) of NaOH alone or in combination with 2 levels (130 or 260 g/kg straw DM) of H2O2 (alkaline hydrogen peroxide, AHP) in improving in vitro dry matter digestibility of wheat straw. The study also examined the role of filtration or a water wash of straws treated at a regulated pH (± s.d.) of 11.5 ± 0.2 to maximise the effects of NaOH and AHP on in vitro dry matter digestibility. The experiment was a 3 × 2 × 3 factorial design, replicated twice, and included 3 straw groups (pH-control, NaOH-control, AHP), each treated with 2 volumes of chemical solutions (high, 26 L/kg DM containing 48 or 160 g NaOH alone or 160 g NaOH plus 260 g H2O2 ; low, 13 L/kg DM containing 24 or 80 g NaOH alone or 80 g NaOH plus 130 g H2O2 ) and each was subdivided into 3 groups for storage (unfiltered, filtered, washed). Both NaOH-control and AHP reduced (P<0.001) neutral detergent fibre content and increased (P<0.001) in vitro dry matter digestibility of straw compared with pH-control. However, AHP was more effective (P<0.001) in reducing neutral detergent fibre and increasing in vitro dry matter digestibility of straw compared with NaOH alone. Filtration and washing of treated straws increased (P<0.001) neutral detergent fibre and reduced (P<0.001) in vitro dry matter digestibility compared with unfiltered samples of treated straws. High volumes of chemicals were more effective (P<0.001) in reducing neutral detergent fibre content and improving in vitro dry matter digestibility of straws compared with those treated with low volumes of chemicals. While regulation of pH around 11.5 was effective in reducing neutral detergent fibre and enhancing in vitro dry matter digestibility the use of filtration or a water wash of straws following chemical treatments is not recommended.

Australian and Spanish scientists are collaborating in the domestication of tedera: young Merino sheep grazing a monoculture of tedera in autumn showed preference for certain accessions but no signs of ill health

2013 ◽  
Vol 64 (4) ◽  
pp. 399 ◽  
Author(s):  
C. Oldham ◽  
D. Real ◽  
H. J. Bailey ◽  
D. Thomas ◽  
A. Van Burgel ◽  
...  
Keyword(s):  
Selection Index ◽  
Blood Analysis ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Sheep Grazing ◽  
Neutral Detergent Fibre ◽  
Relative Preference ◽  
Condition Score ◽  
Acid Detergent Fibre

We hypothesised (i) that sheep grazing a monoculture of tedera (Bituminaria bituminosa (L.) C.H. Stirton var. albomarginata and var. crassiuscula) would not show signs of photosensitisation or ill health, and (ii) that when given free grazing choice they would show a repeatable preference for certain accessions of tedera related to their chemical composition. We tested this by grazing a group of young merino wethers on a monoculture containing seven accessions of tedera for 21 days. General health was assessed via daily visual checks for skin pinkness on the nose and ears, weekly measures of liveweight, condition score, and blood analysis compared with a group of control sheep fed wheaten hay ad libitum. The Chesson–Manly selection index was used to examine the relative preference of sheep for the seven accessions of tedera over the 21 days. Each accession of tedera was sampled weekly to estimate the dry matter on offer, and these samples were also analysed for crude protein, neutral detergent fibre, acid detergent fibre, in vitro digestibility, water soluble carbohydrates, minerals, and concentrations of the furanocoumarins psoralen and angelicin. None of the sheep showed any signs of ill health, with all blood parameters being within the normal reference range. All sheep gained weight and body condition over the 21 days. The difference in the rate of gain in condition score in favour of the sheep grazing tedera over the 21 days (0.014 v. 0.002 unit/sheep.day) was significant (P < 0.001). Sheep showed repeated preference for accessions T31 and T43 (α >0.143). Nutritive value of all accessions of tedera was high. However, only acid detergent fibre and neutral detergent fibre affected the relative preference of the sheep (P < 0.05) and they were only weakly correlated (r2 = 0.208 and 0.165, respectively). We conclude that there are accessions of tedera that are preferred by sheep that could be used to fill the autumn feed gap experienced in the south of Western Australia without any risk to the health of the sheep.

Upgrading wheat straw with urea at a tropical temperature: effects of amount of urea and moisture on in vitro digestibility and pH

1996 ◽  
Vol 1996 ◽  
pp. 212-212
Author(s):  
I.U. Haq ◽  
E. Owen
Keyword(s):  
Wheat Straw ◽  
Urea Solution ◽  
In Vitro Digestibility ◽  
Ammonia Treatment ◽  
Urea Treatment ◽  
Cow Urine ◽  
The Cost ◽  
The Tropics ◽  
On Farm

Urea-ammonia treatment of straws in the tropics involves mixing 1.0 kg of air dry straw with 1.0 kg of a 40 g/kg urea solution and storing under plastic for at least 4 weeks (Schiere and Ibrahim, 1989). The economics of treatment is dependent on the cost of urea. Treatment cost would reduce, if on-farm-produced urine, e.g. cow urine, could be used as a source of urea. However cow urine is dilute and may contain only 10 g/kg urea or less (Owen, 1993). The present study therefore investigated varying concentrations of urea solution for treating wheat straw at a tropical temperature.

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

Cultivation of housefly larvae (Musca domestica L.) as a biological method to enrich rice straw and maximise its utilisation

2021 ◽  
pp. 1-12
Author(s):  
F.M. Abo-Donia ◽  
U.A. Nayel
Keyword(s):  
Rice Straw ◽  
Musca Domestica ◽  
Crude Protein ◽  
In Vivo Studies ◽  
Feed Consumption ◽  
Neutral Detergent Fibre ◽  
Biological Technique ◽  
Acid Detergent Fibre

This study is aimed at presenting a new biological technique to enrich rice straw via the cultivation of Musca domestica (MD) and at evaluating the outcome of this technique using in vitro and in vivo studies. Rice straw was treated using molasses and water at 17.5: 0.375: 7.0 (w/v/v), respectively. The mixture was left in an open shaded area for 11 successive days to allow MD to lay eggs and produce larvae. During this time, the recorded temperature was 35.6±2.2 °C and the humidity was 49±5.7%. Rice straw treated with 4% urea (URS) and untreated rice straw (RS) were used for comparison with in vitro and in vivo MD maggot rice straw (MRS). The crude protein (CP) and ether extract (EE) contents gradually increased during the first 8 days, and then began to gradually decrease until the 11th day. In vitro studies were performed on RS, MRS and URS as a single substrate. Instead, in the in vivo studies, experimental RS was offered ad libitum to sheep, and a concentrate feed mixture was provided as pellets to cover the maintenance requirements. The volume of produced gas and the in vitro degradability of the organic matter (OM), ash-free neutral detergent fibre, and CP (P<0.05) increased with the fermentation of the mg of MRS, compared to URS, which in turn was (P<0.05) higher than that of RS incubation. The consumed MRS was significantly (P<0.05) higher than the consumed URS. At the same time, the URS consumption was highly (P<0.05) significant, compared to the RS consumption. The eating, ruminating and chewing time (min/head/d) was significantly (P<0.05) higher when RS was consumed, compared to URS and MRS. Eating a diet including MRS led to higher (P<0.05) dry matter, OM, CP, EE, neutral detergent fibre, and acid detergent fibre digestibility, than the diet containing URS, which was significantly (P<0.05) superior to the diet containing RS. The total digestible nutrients (TDN) and digestible crude protein values of the MRS-containing diet improved (P<0.05) more than that of URS, which was significantly (P<0.05) higher than RS. In short, the results indicate that MD maggots could be produced on rice straw to elevate the protein content and nutritional value of feeds. Moreover, this technique improved the feed consumption and digestion coefficients more than the treated and untreated rice straw.

Variability in the chemical composition of cereal straws and in vitro digestibility with and without sodium hydroxide treatment

1979 ◽  
Vol 19 (98) ◽  
pp. 350 ◽  
Author(s):  
GR Pearce ◽  
J Beard ◽  
EP Hilliard
Keyword(s):  
In Vitro Digestibility ◽  
Sodium Potassium ◽  
Factors Affecting ◽  
Wide Range ◽  
Acid Detergent Fibre ◽  
Gross Energy ◽  
Calcium Magnesium ◽  
Straw Quality ◽  
Sodium Hydroxide Treatment

A total of 28 samples of wheat, oat and barley straws showed a wide range in concentration of nitrogen, ash, gross energy, neutral detergent fibre, acid detergent fibre, lignin, cellulose, hemicellulose, residual ash, calcium, magnesium, sodium, potassium and phosphorus. Four straws that had suffered rain damage before collection ranged in in vitro organic matter digestibility from 25 to 34%, compared with 30 to 49% for the other straws; after treatment with 6 g NaOH per 100 g dry matter the ranges were 42 to 46% and 43 to 62%, respectively. The need to determine the effects of the various factors affecting straw quality is emphasized.

Effects of increasing the inclusion level of Atriplex amnicola in the diet of sheep

2009 ◽  
Vol 49 (11) ◽  
pp. 1029
Author(s):  
M. B. P. Kumara Mahipala ◽  
G. L. Krebs ◽  
P. McCafferty ◽  
K. Dods
Keyword(s):  
Crude Protein ◽  
Energy Content ◽  
Gas Production ◽  
Mineral Contents ◽  
Neutral Detergent Fibre ◽  
Freeze Dried ◽  
Acid Detergent Fibre ◽  
Inclusion Level ◽  
Metabolisable Energy

The effects of increasing the level of inclusion of fresh Atriplex amnicola Paul G.Wilson foliage in an oaten chaff (Avena sativa L.) diet fed to sheep were investigated. Six experimental diets were formulated to contain varying amounts [0, 161, 362, 496, 650 and 836 g/kg diet dry matter (DM)] of A. amnicola, and these diets were fed to individually penned sheep according to a Latin square design. Feed and faecal samples were analysed for crude protein, neutral detergent fibre, acid detergent fibre, acid detergent lignin, and ash contents. Mineral contents and 24 h in vitro gas production were measured in freeze-dried feed samples. As the level of inclusion of A. amnicola increased, digestibility of DM, organic matter and neutral detergent fibre increased (P < 0.05), reaching the peak at the 496 g/kg DM inclusion level and then decreasing (P < 0.05) thereafter. Acid detergent fibre digestibility followed a similar trend, but peak digestibility was at 362 g/kg DM inclusion level. Digestibility of crude protein continued to increase (P < 0.05) with increasing inclusion of A. amnicola. Gas production (24 h) from A. amnicola was lower (P < 0.05) than that from oaten chaff (24.4 v. 45.3 mL/200 mg DM) and also had lower metabolisable energy content (in vitro ME, 6.3 v. 8.6 MJ/kg DM). As the level of inclusion of A. amnicola in the diet increased, gas production and in vitro metabolisable energy content decreased (P < 0.05). The 496 g/kg DM inclusion level provided the optimum N : energy ratio for fermentation of dietary fibre. At this level of inclusion the diet was rich in Na, Ca, P, Mg, K, S, Mn and Zn. High inclusion levels improved the crude protein value of the diet but compromised fibre digestibility.

Effect of fungal treatment on the chemical composition, in vitro digestibility and in sacco degradability of wheat straw

2003 ◽  
Vol 2003 ◽  
pp. 166-166
Author(s):  
H. Fazaeli ◽  
A. Azizi ◽  
Z. A. M. Jelan ◽  
S. A. Mirhadi
Keyword(s):  
Chemical Composition ◽  
Wheat Straw ◽  
Nutritive Value ◽  
White Rot Fungi ◽  
White Rot ◽  
In Vitro Digestibility ◽  
Fungal Treatment ◽  
In Sacco Degradability ◽  
In Sacco

Fungal treatment has been recently considered as a promising method for improving the nutritive value of straw (Zadrazil et al., 1997). Several studies have been conducted to identify species of white-rot fungi for assessing their ability to improve the nutritive value of straw (Yamakamwa et al., 1992). Since there are many species of fungi in nature, there is an interest in characterising of some species. The objectives of this experiment were to study the effect of five Pleurotus fungi on the chemical composition, in vitro digestibility and in sacco degradability of wheat straw and evaluate their effect in upgrading the nutritive value of lignicellulosic materials.

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