scholarly journals Effects of auxin and cytokinin levels on the success of air layering in tea plant clones of GMB 7 and GMB 9 using husk charcoal, cocopeat and moss media

2020 ◽  
Vol 5 (2) ◽  
pp. 86
Author(s):  
Ika Betty Widyastuti ◽  
Prapto Yudono ◽  
Eka Tarwaca Susila Putra

The research aimed to propagate tea plants by air layering in order to obtain new plants with shorter immature plants period (TBM), which is 1.5 years, by utilizing wasted branches from routine clean pruning activities in tea plantations. The research was conducted from August 2018 to January 2019 at tea plantation of PT. Pagilaran, Batang, Central Java. Experiment using single factor treatment was arranged in a Completely Randomized Design with three replications. The treatment was layering media, consisting of husk charcoal, moss, and cocopeat, which were applied on GMB 7 and GMB 9 clones. The results showed significant effects of the layering media on the levels of auxin and cytokinin in GMB 7. The highest levels were found in the husk charcoal and moss media. Analysis of sucrose, glucose and total sugar as well as the physiological analysis of the air layering roots showed no significant difference in the fresh weight, dry weight, volume, surface area, diameter, and length of the roots. Husk charcoal resulted in the highest success rate of the air layering in GMB 7, which was 100%. Meanwhile, GMB 9 showed significant difference only in the auxin levels. There was no significant effect of layering media on the analysis of sucrose, glucose and total sugar as well as on the physiological analysis of the air layering roots. Both husk charcoal and moss media resulted in the highest success rate of the air layering in GMB 9, which was 58.33%.   

2021 ◽  
Vol 26 (1) ◽  
pp. 113-119
Author(s):  
Ika Betty Widyastuti ◽  
Prapto Yudono ◽  
Eka Tarwaca Susila Putra

The large number of old, damaged, or dead tea plants cause the plant population per hectare to be low that eventually decreases the productivity. Propagation using the transplantation technique is used for embroidery because it has the advantage of a shorter immature plant period and higher seed size so that it can compensate for the surrounding plants but the weakness of transplantation is low success rate. This study aims to 1) determine the morphological changes of root transplantation and biochemistry on TRI 2025 clone transplantation using cocopeat, husk charcoal, and moss, 2) determine the success rate of transplantation in each medium. This research was conducted in August 2018–January 2019 at the Pagilaran production garden, PT. Pagilaran, Batang, Central Java. Field experiments were prepared using a single factor Completely Randomized Environment Design (CRD). The factors tested were the type of transplantation media, namely the media of husk charcoal, cocopeat, and moss. Observations were made on transplantation root morphology, levels of glucose, sucrose, and total sugar in the transplanted stem bark and the concentration of auxin, gibberellin, and cytokinin in the transplant roots. The data obtained were then analyzed using variance and if there was a significant difference between treatments, the Duncan multiple distance test (DMRT) was continued at the 95% confidence level. The results showed that the type of media had no significant effect on the ability to form glucose, sucrose, and total sugar in the bark. The development of root morphology of cocopeat media was seen to be the highest. The ratio of cytokinin/auxin in cocopeat was the lowest but the highest levels of cytokinin and auxin were found in moss, while cocopeat had the highest level of gibberellin. The highest transplanting success rate was found in moss medium with 58.33%, cocopeat and husk charcoal media had the same success rate, namely 41.60%.   Keywords: transplantation, biochemistry, media, success, TRI 2025


2014 ◽  
Vol 1 (1) ◽  
pp. 25-29
Author(s):  
Rahim Mohammadian ◽  
Behnam Tahmasebpour ◽  
Peyvand Samimifar

A factorial experiment was conducted with a completely randomized design to evaluate the effects of planting date and density on calendula herbs and peppermint. It had 3 replicates and was done in Khosroshahr research farm, Tabriz in 2006. Under studied factors were: 3 planting dates (10 May, 25 May and 10 June) in 4 densities (25, 35, 45, 55) of the plant in square meters. The results of variance a nalysis showed that there was 1% probability significant difference between the effects of planting date and bush density on the leave number, bush height and the bush dry weight. But the mutual effect of the plant date in mentioned traits density was insignificant. Regarding the traits mean comparison, the total maximum dry weight was about the 55 bush density in mm. Also, the bush high density in mm causes the bush growth and its mass reduction. When there is the density grain, the flower number will increase due to bush grain in surface unit. Overall, we can conclude that 10 June planting and 45 bush density in mm is the most suitable items and results in favored production with high essence for these crops.


2019 ◽  
Vol 5 (2) ◽  
pp. 41
Author(s):  
Yetti Elidar

The research objective was to determine the effect of water and the volume of urea fertilizer and the interaction of both the oil palm plant roots in the nursery early (pre nursery). This research was conducted in the Nursery Faculty of Agriculture, University of Mulawarman, Samarinda from February to May 2014. The study using completely randomized design (CRD) factorial 3 x 4 with three replications. The first factor is the volume of water (A) in the planting medium consists of three levels, namely: a1 = 100 cc of water plants-1; a2 = 200 cc of water plants-1; a3 = 300 cc water plant-1. The second factor is the provision of urea fertilizer (P), which consists of four levels, namely: p0 = without fertilizer urea; p1 = urea 1 g L-1 water to 100 seedlings; p2 = urea 2 g L-1 water to 100 seedlings; PP3 = urea 3 g L-1 water to 100 seedlings. Number of treatment were 12 and each treatment was repeated three times and each replication consisted of three plants so the total number is 108 plants. Data were statistically analyzed and tested further by testing the smallest Significant Difference (LSD) at 5% level. The results showed that the water volume of 200 cc of plant-1 (p2) provides the highest root dry weight average of 254.13 g. While the interaction between the water volume of 200 cc of plant-1 and dose of urea 3 g L-1 water (a2p3) provides the highest root dry weight average of 300,00 g.


Author(s):  
Dwi Astutik ◽  
Ratih Rahhutami ◽  
Aline Sisi Handini ◽  
Ahmad Sutopo

<em>A planting medium that rich in nutrients is needed for oil palm, especially in the nursery phase. This study aimed to determine the effect of various doses of EM4 and to get the best dose of EM4 on the growth of oil palm seedlings in the pre-nursery. The research method used was a non-factorial completely randomized design with 4 treatments, namely 0 ml EM4 (A1), 4 ml EM4 (A2), 8 ml EM4 (A3), and 12 ml EM4 (A4). Each treatment was repeated 6 times so that there were 24 experimental units. The data obtained were analyzed for variance at the 5% level, if the effect was real, it was followed by the least significant difference (LSD) test. Based on the results of the study, it could be concluded that application of various EM4 doses had a significant effect on plant height (4-12 MST), the number of leaves (4-8 MST), trunk girth (4-12 MST), and shoot wet weight but did not significantly affect the number of leaves (12 MST), the volume of roots, length of roots, root wet weight and shoot dry weight, dry weight root, the best dose was shown at a dose of 12 ml EM4.</em>


2021 ◽  
Vol 9 (3) ◽  
pp. 262
Author(s):  
Alawiyah Alawiyah ◽  
Slamet Budi Yuwono ◽  
Melya Riniarti ◽  
Dermiyati Dermiyati ◽  
Christine Wulandari

Open mining activities have caused soil damage, water pollution, air pollution, and damage to vegetation. Limestone post mining is a critical area that must be rehabilitated to be productive again. This problems can be fixed by doing land reclamation activities which mandated in the Mining Law Number 4, 2009. The success of land reclamation is largely determined by the choice of plant species and the provision of appropriate soil enhancers (ameliorant). This study was aimed to analyze the growth response of sengon plants on giving ameliorant in mixed limestone post mining soil. This study was designed in completely randomized design for 7 treatments and 3 replications used combination of ameliorant: P0 (without ameliorant); P1 (AH 4 kg ha-1); P2 (BFA 350 kg ha-1); P3 (FMA 500 spores plant-1); P4 (the combination of AH and FMA); P5 (the combination of BFA and FMA); and P6 (the combination of AH, BFA, and FMA). Data were processed by analysis of variance at the 0,05 significance level followed by the Least Significant Difference test. The results showed that the best growth response of sengon on giving ameliorant in mixed limestone post mining soil was P1 treatment (humic acid material) which significantly increased root length, root volume, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight in sengon plants. The addition of ameliorant can be recommended to improve the quality of revegetation plants


2020 ◽  
Vol 7 (1) ◽  
pp. 62-66
Author(s):  
Masniar Vina Kesti ◽  
Bambang Irawan ◽  
Priyambodo Priyambodo ◽  
Martha Lulus Lande

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.


2020 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Icha Rusita ◽  
Hadi Sasongko

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.


METANA ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 55-60
Author(s):  
Edy Supriyo ◽  
Isti Pudjihastuti

Teh merupakan minuman yang dihasilkan dari pucuk daun tanaman Camellia sinensis  yang tumbuh di pegunungan pada ketinggian 600–2500 m dpl. Teh hitam merupakan jenis teh yang banyak dikonsumsi masyarakat Indonesia. Teh ini diproduksi dengan cara fermentasi melalui proses oksidasi enzimatis katekin oleh polifenol oksidase. Teh hitam yang dikemas dalam bentuk the celup banyak digemari konsumen.  Tujuan dari penelitian ini mengetahui konsentrasi polyfenol dalam teh hitam celup komersial. Sampel teh hitam celup komersial dengan merk dagang TP, TDT, TB, TL,  TD, dan TM diproduksi oleh enam perkebunan teh di Jawa Tengah yang diperoleh secara acak dari swalayan di Kota Semarang.   Kandungan polyfenol pada sampel teh hitam celup dianalisa dengan menggunakan spetrofotometer UV-Vis pada panjang gelombang 725nm. Hasil penelitian menunjukkan bahwa teh hitam celup komersial yang beredar di Kota Semarang telah memenuhi  standard SNI 3753–2014, dengan rata-rata konsentrasi polyfenol 8,83-43,63 %b/b. Analisa Zscore  menunjukkan tidak ada perbedaan yang sangat nyata antar konsentrasi polyfenol di enam sampel teh hitam celup komersial, hal ini dimungkinkan dengan adanya standarisasi proses produksi teh hitam di berbagai industri teh di Indonesia. Dan teh hitam celup komersial TDT mempunyai konsentrasi polyfenol diatas rata-rata yaitu 43,63 % b/b.    Tea is a drink produced from the leaves of the Camellia sinensis plant that thrives in the mountains at an altitude of 600–2500 m above sea level. Black tea is a type of tea that is widely consumed by Indonesian people. Black tea is produced by fermentation, namely the process of enzymatic oxidation of catechins by polyphenol oxidase. Black tea is produced in several packages, including as black tea bags. The purpose of this study was to determine the concentration of polyphenols in commercial black tea bags. Samples of commercial black tea bags with the trademarks TP, TDT, TB, TL, TD, and TM were produced by six tea plantations in Central Java which were obtained randomly from supermarkets/stores in Semarang City. The polyphenol content in black teabag samples was analyzed using a UV-Vis spectrophotometer at a wavelength of 725nm. The results showed that the commercial black tea bags marketed in the Semarang City had met the standards of SNI 3753–2014, with an average polyphenol concentration of 8.83-43.63% w/w. The Zscore analysis showed that there was no significant difference between the concentrations of polyphenols in the six samples of commercial black tea bags, this may cause by the standardization of black tea production processes in various tea industries in Indonesia, but commercial black tea bags with the trademark TDT had polyphenol concentrations above the average is 43.63% w/w.


2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Hossain Sohrawardy ◽  
Md. Lokman Hossain

The study was conducted as a pot experiment in the tropical glasshouse to evaluate the response of grain legumes (Phaseolus vulgaris, Vigna unguiculata, andLablab purpureus) in comparison to maize (Zea mays) and estimate their potential and performance. Two experiments were established using completely randomized design. Physiological measurements (stomatal conductance, photosynthetic activities, and transpiration rates) were measured using LCpro instrument. Scholander bomb was used for the measurement of plant cell water potential. Significant difference was observed in different plant species with increase of different water regimes. Among the legumes,L. purpureusshowed better response in water stressed conditions. At the beginning, in dry watered treatment the photosynthetic rate was below 0 µmol m−2 s−1and in fully watered condition it was 48 µmol m−2 s−1. In dry treatment, total dry weight was 10 g/pot and in fully watered condition it was near to 20 g/pot inP. vulgaris. The study concludes that water stress condition should be taken into consideration for such type of crop cultivation in arid and semiarid regions.


2021 ◽  
Vol 316 ◽  
pp. 03007
Author(s):  
Sutarman ◽  
A. Miftahurrohmat

This study aims to determine the growth response of the vegetative stadia of three soybean varieties which are widely used in East Java on acid soils that have been given biological fertilizers from various Trichoderma isolates. The experiment was arranged factorially using a completely randomized design. The first factor was the variety consisting of Detam 3, Detam 4, Gema, Dering 1, and Burangrang. The second factor was Trichoderma isolates formulated as bio fertilizers, consisting of Tc-Jjr-02, Tc-Pjn-01, and Tc-Jro-01. Overall this experiment has 60 experimental units. Data were analyzed by variance analysis (ANOVA) followed by an honest significant difference test at the level of 5%. The results showed that there was no interaction between soybean varieties and Trichoderma isolates formulated in bio fertilizer. Soybean varieties significantly affected plant height, wet weight, and dry weight of roots and stover at 21 days after planting. The Burangrang variety shows the best growing ability in acid soils. There was no interaction between soybean varieties and Trichoderma bio fertilizers. Trichoderma isolate Tc-Jjr-02 increased the wet weight and dry weight of the roots and roots by 48.2 and 54.5%, respectively, and 38.9 and 48.2% compared to without Trichoderma. Trichoderma application maintains soil acidity between pH 4.50-4.67.


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