The Effect of Vitamin E Supplement in the Diet on Gonad Maturation of Nilem (Osteochilus hasselti, CV)

The aim of this study was to examine the effect of vitamin E on gonad maturation and determining the best doses in improving gonad maturation in nilem fish. In this study used a flockof fishbrood which is never experienced spawning. There are 4 doses of vitamin E which used in this research following by 0, 125, 250, and 375 mg/kg. The results showed that fortifiedof vitamin E which were in feed significantly affected to the percentage of gonad development, gonadosomatic index (GSI), hepatosomatic index (IHS), fecundity, the diameter of eggs, and the percentage of egg maturity at P<0.05. The result showed that the best dose of vitamin E to reach gonad maturity was 375 mg kg-1 with six weeks maintenance. The percentage of gonad maturity was 100%, IGS was 11.50%, IHS was 0.34%, fecundity was 23.484 eggs/individual, the diameters of the eggs were 0.31 mm, and the percentage of mature eggs was 64.44%. These results supported the potential of feed additives such as vitamin E plays a critical role in enhancing gonad maturation in nilem fish thereby providing methods to improve fish reproduction

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Penggunaan serotonin dalam formulasi hormon pregnant mare serum gonadotropin dan antidopamin untuk menginduksi perkembangan gonad ikan ringau, Datnioides microlepis Bleeker, 1854 [Serotonin application in pregnant mare serum gonadotropin hormone and dopamin antagonist formulation to induce gonadal development of Indonesian tigerfish (Datnioides microlepis Bleeker, 1854)]

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v17i1.302 ◽

2017 ◽

Vol 17 (1) ◽

pp. 29

Author(s):

NFN Bastiar ◽

Agus Oman Sudrajat ◽

Melta Rini Fahmi

Keyword(s):

Plasma Concentration ◽

Gonadosomatic Index ◽

Gonad Development ◽

Gonadal Development ◽

Hepatosomatic Index ◽

Small Fish ◽

Pregnant Mare Serum Gonadotropin ◽

Randomized Design ◽

Serum Gonadotropin ◽

Completely Randomized Design

The objective of study was to analyze the effect of serotonin (5-HT) in the formulation of hormones Pregnant Mare Serum Gonadotropin (PMSG) and dopamin antagonist (AD) hormones to gonad development of tigerfish. This study used completely randomized design (CRD) with five treatments of hormone namely: (P1) 1 ml of 0.9% NaCl (control); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG AD+10 mg+0.2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; and (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Each treatment was tested on five fish as individual replications. Hormone injected intramuscularly at the lower part of the dorsal fin of fish every 10 days. The fish were reared for 60 days. Fish that were used at this study were originate from natural catches as much as 25 fishes with 17.5-33.0 cm of total length and 118-926 g of body weight. During the study, fish fed using shrimp and small fish (live) twice daily at satiation. Measured parameters were gonadosomatic index (GSI), hepatosomatic index (HSI), 17P-estradiol (E2) plasma concentration and gonad maturity level based on morphology and histology examination. The results showed that the use of 2 mg of 5-HT are added to 20 IU PMSG and 10 mg AD (treatment P4) has stimulated the fish to had the highest GSI (2.38 ± 0.06%) and HSI (3,09±0,12%) which was significantly different to other treatment. The treatment (P4) could increase the E2 plasma concentration (37.14±2.99 pg.ml-1) two fold compared with the concentration before injection and stimulated the gonadal development to stage III. AbstrakPenelitian ini bertujuan untuk menganalisis pengaruh penggunaan serotonin (5- hydroxytryptamine atau 5-HT) dalam formulasi hormon Pregnant Mare Serum Gonadotropin (PMSG) dan antidopamin (AD) terhadap perkembangan gonad ikan ringau. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan (dosis.kg-1 bobot tubuh ikan): (P1) 1 ml NaCl 0,9% (kontrol); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG+10 mg AD+0,2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; dan (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Setiap perlakuan diujikan pada lima ekor ikan sebagai ulangan individu. Penyuntikan hormon dilakukan setiap 10 hari dengan lama penelitian 60 hari. Hormon disuntikkan secara intramuskular pada bagian bawah sirip punggung ikan uji. Ikan yang digunakan merupakan hasil tangkapan alam dengan ukuran panjang total 17,5-33,0 cm dan bobot tubuh 118-926 g. Selama penelitian, ikan uji diberi pakan berupa udang dan ikan-ikan kecil (hidup) dua kali sehari secara satiasi. Parameter yang diamati adalah indeks kematangan gonad, indeks hepatosomatik, konsentrasi estradiol-17p plasma dan tingkat kematangan gonad berdasarkan morfologi dan histologi gonad. Hasil penelitian menunjukkan penggunaan 2 mg 5-HT dalam 20 IU PMSG dan 10 mg AD (perlakuan P4) menghasilkan perkembangan gonad yang lebih baik dibandingkan perlakuan lainnya. Nilai indeks kematangan gonad, indeks hepatosomatik, dan konsentrasi E2 plasma tertinggi diperoleh pada perlakuan P4 dengan nilai masing-masing sebesar 2,38±0,06%; 3,09±0,12% dan 37,14±2,99 pg.ml-1. Nilai tersebut berbeda nyata p<0.05) dengan perlakuan lainnya. Perlakuan P4 meningkatkan konsentrasi E2 plasma dua kali lebih besar dibanding-kan sebelum penyuntikan serta menghasilkan perkembangan gonad yang mencapai tingkat kematangan gonad tahap III.

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Reproductive biology of the four dominant species of catfish from the Persian Gulf

Animal Biology ◽

10.1163/15707563-20191108 ◽

2020 ◽

Vol 70 (2) ◽

pp. 131-143

Author(s):

Farideh Mohseni ◽

Tooraj Valinassab ◽

Ehsan Ramezani-Fard ◽

S.M. Reza Fatemi ◽

Mohammad Seddiq Mortazavi

Keyword(s):

Sex Ratio ◽

Breeding Season ◽

Persian Gulf ◽

Gonadosomatic Index ◽

Gonad Development ◽

Common Species ◽

Hepatosomatic Index ◽

Essential Information ◽

Total Length ◽

The Persian Gulf

Abstract Catfish are an important component of demersal fisheries in the Persian Gulf. This study describes reproductive aspects in the four common species of catfish, namely Netuma thalassina (Rüppell, 1837), Netuma bilineata (Valenciennes, 1840), Plicofollis dussumieri (Valenciennes, 1840), and Plicofollis layardi (Günther, 1866) collected from coastal areas of the Persian Gulf during spring and winter 2017. A total of 495 fishes were collected, including 151 N. thalassina, 12 N. bilineata, 212 P. dussumieri, and 120 P. layardi. The four species showed significant differences in total length as a measure of body size. Total length of these four species also differed significantly between the four seasons and between the sexes. Three species, i.e., N. thalassina, P. dussumieri, and P. layardi showed an overall female-biased sex ratio, while the sex ratio of N. bilineata did not differ from the expected equal proportions. The patterns of gonad development, gonadosomatic index, and hepatosomatic index of the four species showed that the breeding season of N. thalassina, P. dussumieri, and P. layardi was probably during winter. N. bilineata showed a different pattern; its breeding season is therefore expected to be spring. The size at first maturity of N. thalassina, P. dussumieri, N. bilineata, and P. layardi, was 44.99, 30.55, 29.60, and 27.01 cm, respectively. This study provides essential information for correct management during the reproductive cycle to maintain a healthy stock of these fishes.

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MORPHOMETRIC ANALYSIS AND REPRODUCTIVE BIOLOGY OF THE CRAB CHARYBDIS AFFINIS (DECAPODA, BRACHYURA, PORTUNIDAE) FROM THE ZHUJIANG ESTUARY, CHINA

Crustaceana ◽

10.1163/156854099503690 ◽

1999 ◽

Vol 72 (7) ◽

pp. 647-658 ◽

Cited By ~ 17

Author(s):

Keyword(s):

Sexual Difference ◽

Gonadosomatic Index ◽

Carapace Width ◽

Gonad Development ◽

Hepatosomatic Index ◽

Morphometric Characters ◽

Rate Analysis ◽

Gonadal Maturity ◽

Different Seasons ◽

Zhujiang Estuary

AbstractMorphometric characters and organ indices were analyzed in Charybdis affinis collected at different seasons from the Zhujiang estuary, China. The crab is heterochelous, with pronounced right handedness in both sexes. There is a marked sexual difference in relative growth of the cheliped. The female abdomen increases in size at puberty, which is also accompanied by a reduced allometric growth rate. Analysis of gonad development gave the estimate of 50% gonadal maturity at 42 and 36 mm carapace width for females and males, respectively. The hepatosomatic index of female crabs is inversely related to the gonadosomatic index indicating the role of the hepatopancreas in storage of nutrients necessary for ovarian development in the reproductive season. Les caracteres morphologiques et les indices relatifs aux organes ont ete analyses chez Charybdis affinis, recolte a differentes saisons dans l'estuaire du Zhujiang, Chine. Le crabe presente une heterochelie, la pince droite etant tres developpee dans les deux sexes. Il existe une difference sexuelle marquee dans la croissance relative du chelipede. L'abdomen femelle s'accroot en taille a la puberte, ce qui s'accompagne aussi d'un taux de croissance allometrique reduit. L'analyse du developpement des gonades a donne une maturite estimee a 50% pour des femelles et males, respectivement, ayant une largeur de la carapace de 42 et 36 mm. L'indice hepatosomatique des crabes femelles est inversement proportionnel a l'indice gonadosomatique ce qui indique le role de l'hepatopancreas dans le stockage des nutriments necessaire au developpement ovarien lors de la reproduction.

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Hormonal induction maturation of silver pompano Trachinotus blochii

Jurnal Akuakultur Indonesia ◽

10.19027/jai.19.1.61-73 ◽

2020 ◽

Vol 19 (1) ◽

pp. 61-73

Author(s):

Wiwin Kusuma Atmaja Putra ◽

Tengku Said Raza’i

Keyword(s):

Body Weight ◽

Gonadosomatic Index ◽

Hormone Treatment ◽

Hepatosomatic Index ◽

Gonad Maturation ◽

Maturity Level ◽

Randomized Design ◽

Absolute Growth ◽

Completely Randomized Design ◽

Trachinotus Blochii

ABSTRACT This study was aimed to acquire the most suitable hormone treatment to induce gonad maturation of silver pompano Trachinotus blochii. This study used a completely randomized design with four treatments and three replications, i.e NaCl 90% (control) (0.5 mL/kg), hCG (20 IU/kg fish body weight), PMSG (20 IU/kg fish body weight), and PG600 (20 IU/kg fish body weight). Parameters measured were gonadosomatic index (GSI), hepatosomatic index (HSI), gonad maturity level (TKG), gonad histology, and absolute growth. The best result was PG600 treatment with 0.12% gonadosomatic index, 2.29% hepatosomatic index, TKG IV, and 85 g absolute growth for four weeks. This study concludes that the most suitable hormone to induce gonad maturation of silver pompano Trachinotus blochii is PG600 with 20 IU/kg body weight, which effectively affects GSI, HSI, and absolute growth of silver pompano. Keywords: hCG, maturation, PG600, PMSG, silver pompano. ABSTRAK Penelitian ini bertujuan untuk mendapatkan hormon yang terbaik untuk menginduksi maturasi gonadikan bawal bintang. Hormon yang digunakan pada penelitian ini adalahkontrol NaCl 90% (dosis 0,5 mL/kg), hCG(dosis 20 IU/kg bobot tubuh ikan), PMSG (dosis 20 IU/kg bobot tubuh ikan), dan PG600 (dosis 20 IU/kg bobot tubuh ikan). Parameter yang diamati adalah indeks gonadosomatik (GSI), indeks hepatosomatik (HSI), tingkat kematangan gonad (TKG), histologi gonad, dan pertumbuhan bobot mutlak. Hasil terbaik adalah perlakuan PG600 dengan gonadosomatik indeks (0,12%), hepatosomatik indeks (2,29%), TKG IV,dan pertumbuhan mutlak (85 g) selama empat minggu. Kesimpulan penelitian ini adalah perlakuan hormone yang berpotensi menginduksi maturasi gonad ikan bawal bintang adalah hormon PG600 dengan dosis 20 IU/kg bobot tubuh dikarenakan adanya pengaruh nyata tehadap parameter GSI, HSI dan pertumbuhan mutlak ikan bawal bintang. Kata kunci: ikan bawal bintang, hCG, PMSG, PG600, maturasi.

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Stimulation of gonad maturation in mullet fish Mugil dussumieri using MT, E2, hCG, and Ovaprim hormone

Jurnal Akuakultur Indonesia ◽

10.19027/jai.18.1.9-22 ◽

2019 ◽

Vol 18 (1) ◽

pp. 9-22

Author(s):

Tatak Dwi Cahyono ◽

Muhammad Zairin Junior ◽

Odang Carman

Keyword(s):

Body Length ◽

Egg Size ◽

Gonadosomatic Index ◽

Physiological Solution ◽

Gonad Maturation ◽

Gonad Maturity ◽

And Control ◽

Egg Diameter ◽

Estradiol 17Β ◽

Stimulation Of

ABSTRACT The present study is a preliminary research for producing mullet fry to aquaculture. The research aimed to evaluate stimulation of gonad maturation in mullet (Mugil dussumieri) using hormones. The method used for research was completely randomized design consisting of three treatments and each individual replication was repeated three times. Two experiments were conducted separately with different treatments. First experiment used 9-14.7 cm body length of fish treated with different hormone injections i.e. 4 mg/kg 17α-methyltestosterone (MT), 0.07 mg/kg estradiol-17β (E2), and 0.5 ml/kg 0.9% physiological solution as control. Second experiment used 10-31 cm body length fish treated with 750 IU/kg chorionic gonadotropin (hCG), 0.5 ml/kg Ovaprim, and 0.9% physiological solution as control. Gonadosomatic index (GSI) value of the first experiment on day 60 showed that GSI of E2 treatment (1.31±0.94%) was higher than both MT treatment (1.00±0.51%) and control (0.54±0.20%). On the other hand, the second experiment on day 60 showed that GSI of hCG treatment (7.18±0.59%) was higher than both Ovaprim treatment (3.29±2.66%) and control (6.72±0.32%). Egg diameter frequency distribution for control in the first experiment on day 30 showed that egg size ranged from 9−144 µm. Egg diameter for E2 and MT treatments on day 60 showed that egg size ranged from 9−243 µm and were higher than control. In the second experiment, egg diameter on day 30 for control showed that egg size ranged from 9-144 µm, hCG treatment showed egg size ranged from 9−441 µm, while Ovaprim egg size ranged from 9-111 µm. Blood glucose, blood cholesterol, testosterone and estradiol hormone level in the first and second experiment showed no significant difference. The results showed that estradiol-17β and 17α-methyltestosterone induction in 9-14.7 cm body length mullet increase gonad maturity to stage II while hCG induction in 10-31 cm body length mullet increase gonad maturity to stage III. Keywords : estradiol-17β, hCG, 17α-methyltestosterone, Mugil dussumieri, Ovaprim ABSTRAK Penelitian merupakan rintisan untuk menghasilkan benih ikan belanak dalam wadah budidaya. Penelitian ini bertujuan untuk mengevaluasi pematangan gonad ikan belanak Mugil dussumieri menggunakan hormon. Penelitian dilakukan menggunakan rancangan acak lengkap yang terdiri dari tiga perlakuan dan tiga kali ulangan individu. Terdapat dua percobaan dengan perlakuan berbeda dan dilakukan secara terpisah. Percobaan pertama menggunakan ikan berukuran 9−14.7 cm dengan hormon 17α-metiltestosteron (MT) 4 mg/kg, estradiol-17β (E2) 0.07 mg/kg dan kontrol larutan fisiologis 0.9% 0.5 ml/kg. Percobaan kedua menggunakan ikan berukuran 10−31 cm dengan human chorionic gonadotropin (hCG) 750 IU/kg, Ovaprim 0.5 ml/kg dan kontrol larutan fisiologis 0.9 % 0.5 ml/kg. Nilai gonadosomatic index (GSI) percobaan pertama pada hari ke-60 menunjukkan bahwa pemberian estradiol-17β (1.31±0.94%) lebih tinggi dibandingkan 17α-metiltestosteron (1.00±0.51%) dan kontrol (0.54±0.20%). Hasil percobaan kedua pada hari ke-60 nilai GSI menunjukkan bahwa pemberian hCG (7.18±0.59%) lebih tinggi dibandingkan Ovaprim (3.29±2.66%) dan kontrol (6.72±0.32%). Sebaran frekuensi diameter telur pada percobaan pertama untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm. Sebaran frekuensi diameter telur untuk estradiol-17β dan 17α-metiltestosteron hari ke-60 menunjukkan kisaran 9−243 µm lebih banyak dibandingkan kontrol. Sebaran frekuensi diameter telur pada percobaan kedua untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm, hCG 9−441 µm sedangkan Ovaprim hanya 9−111 µm. Kadar glukosa darah, kolesterol darah, hormon testosteron dan estradiol pada percobaan pertama dan percobaan kedua menunjukkan tidak terdapat perbedaan yang nyata. Hasil penelitian menunjukkan bahwa induksi hormon estradiol-17β dan 17α-metiltestosteron pada ikan berukuran 9−14.7 cm dapat meningkatkan kematangan gonad mencapai TKG II sedangkan induksi hormon hCG pada ikan berukuran 10−31 cm dapat meningkatkan kematangan gonad mencapai TKG III. Kata kunci : estradiol-17β, hCG, 17α-metiltestosteron, Mugil dussumieri, Ovaprim

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Reproductive dynamics of Leporinus obtusidens captured downstream from an important hydropower Station in Brazil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352013000500021 ◽

2013 ◽

Vol 65 (5) ◽

pp. 1419-1426 ◽

Cited By ~ 3

Author(s):

E.S. Andrade ◽

V.O. Felizardo ◽

L.D.S. Murgas ◽

M.M. Drumond ◽

M.G. Zangerônimo ◽

...

Keyword(s):

Condition Factor ◽

Gonadosomatic Index ◽

Reproductive Period ◽

Hepatosomatic Index ◽

Hydroelectric Power Station ◽

Gonad Maturation ◽

Hydroelectric Power ◽

Power Station ◽

Ratio Condition ◽

Males And Females

This study evaluated the reproductive dynamics and gonadal morphology of piaparas (Leporinus obtusidens) captured downstream from the Funil Hydroelectric Power Station. The 107 piaparas evaluated were captured monthly from Sep 2006 to Aug 2007. The data were stratified among the seasons to evaluate gonad maturation and the reproductive period was determined by assessing the gonadosomatic index, hepatosomatic index, abdominal fat index (AFI), weight:length ratio, condition factor and sexual proportion. The reproductive period lasted from winter to summer (Jul-Mar) in females and from spring to autumn (Sept-Jun) in males. AFI was high during and after the reproductive period in females and only during the reproductive period in males. The highest HIS values for males and females were obtained in spring and summer (Sept-Mar). The females observed did not spawn and showed follicular atresia. These characteristics suggest that L. obtusidens does not use the study area as a reproductive site and probably migrates for this purpose.

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Profile of Catfish (Clarias sp) Oocyte Exposed by Laserpuncture

Journal of Biology and Life Science ◽

10.5296/jbls.v5i2.5585 ◽

2014 ◽

Vol 5 (2) ◽

pp. 188

Author(s):

Dyah Hariani ◽

Marhendra A. P. W ◽

Aulanni Am ◽

Suprayitno E

Keyword(s):

Oocyte Maturation ◽

Gonadosomatic Index ◽

Gonad Maturation ◽

Maturity Stage ◽

Oocyte Diameter ◽

Brood Stock ◽

Gonad Maturity ◽

Post Exposure ◽

Egg Diameter

Anexposure of low-powered laserpuncture at reproductive acupoint of catfish brood stock is known stimulate oocyte maturation. However, the profile of oocyte resulted from laserpuncture exposure has not been known. The present study aimed to identify the profile of catfish (Clarias sp.) oocyte in post-exposure laserpuncture. A total of 48 catfishes with ages of 8-9 months was grouped into two, i.e. fishes were exposed with laserpuncture and without exposure to laserpuncture (control) with 3 replications. Laserpuncture exposure was conducted once a week until the brood stock matured. Profile of oocyte such as, the GSI value, egg diameter, total of oocytes and the gonad maturity stage were examined. The results showed that laserpuncture exposure at the catfish reproductive acupoint could trigger the oocyte development without reducing quality of the oocyte indicated by the increase of gonadosomatic index (GSI), oocyte diameter, total of oocytes and gonad maturity stage similar to control. Moreover, laserpuncture exposure accelerated the gonad maturation, three weeks faster than control.

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GONAD DEVELOPMENT OF SANDFISH (Holothuria scabra) FED WITH VITAMIN E-SUPPLEMENTED DIET

Indonesian Aquaculture Journal ◽

10.15578/iaj.14.2.2019.47-54 ◽

2019 ◽

Vol 14 (2) ◽

pp. 47

Author(s):

Sari Budi Moria Sembiring ◽

Jhon Harianto Hutapea

Keyword(s):

Vitamin E ◽

Gonad Development ◽

Water System ◽

Stage Iv ◽

Gonad Maturation ◽

Maturity Stage ◽

Total Biomass ◽

Holothuria Scabra ◽

Gonad Somatic Index ◽

Randomized Design

The experiment was conducted to evaluate the effect of vitamin E on the gonad development of sandfish, Holothuria scabra. Four experimental diets were formulated to contain different levels of vitamin E, i.e. 0, 150, 300, and 450 mg/kg diet. Dry diets were prepared at the same protein level (14%). The experiment was arranged in a completely randomized design with four treatments and three replicates. Before the experiment started, the internal organs of broodstock were removed (evisceration) by injecting KCl 1 N reagent into their body, and the gonads were observed to determine the sex of each broodstock. Mean total length and body weight of broodstock after the internal organ removal were 10.95 ± 0.37 cm and 61.12 ± 2.28 g, respectively. Three broodstock (one male and two females) were allocated into each plastic bucket with a volume of 150 L. Each plastic bucket was equipped with aeration and flow-through water system. Broodstock fed with experimental diet once daily at 3% of total biomass for 90 days. The results showed that vitamin E has significant effects on percentage of gonad maturation stages and gonad somatic index (GSI) (P<0.05). Vitamin E at 300 mg/kg diet was the best for gonad maturation of sandfish broodstock. Furthermore, 55.5% and 11.1% of broodstock fed a diet containing 300 mg vitamin E/kg diet reached gonad maturity stage-III and stage-IV, respectively, with GSI of 12.21%.

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Effect of LH-RHa Injection trough W/O/W LG (C-14) Emulsion on Gonad Development of Catfish (Pangasious hypophthalmus)

Jurnal Akuakultur Indonesia ◽

10.19027/jai.3.15-21 ◽

2007 ◽

Vol 3 (3) ◽

pp. 15

Author(s):

N. Potalangi ◽

M. Toelihere ◽

M. Zairin Junior ◽

Eddy Supriyono

Keyword(s):

Gonad Development ◽

Maximum Size ◽

Oocyte Development ◽

Gonad Maturation ◽

Gonad Maturity ◽

Egg Maturation ◽

Fish Weight ◽

Group B ◽

Egg Diameter ◽

Positive Effect

<p>The objective of this research was to study the effect of LHRHa gonad maturity in broodstock of <em>P. hypophthalmus</em> through W/O/W LG (C-14) emulsion injection. The treatments consisted of control (A), 50 µg/kg fish wight (B), and 100 µg/kg fish weight (C), with five replications of each. Fish weight at the beginning of experiment was 2.0 kg/individual. The result showed that LHRHa in W/O/W emulsion had positive effect on egg maturation. This was shown by the value of average of eggs diameter. The maximum size of egg diameter for fish 701.52 ± 17.56 µm. The size of eggs was more homogenous in group B than those of group C and A. it was concluded that injection of LHRHa in W/O/W LG (C-14) emulsion if effective in promoting gonad maturation and oocyte development in the catfish</p>

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