scholarly journals Profile of Catfish (Clarias sp) Oocyte Exposed by Laserpuncture

2014 ◽  
Vol 5 (2) ◽  
pp. 188
Author(s):  
Dyah Hariani ◽  
Marhendra A. P. W ◽  
Aulanni Am ◽  
Suprayitno E

Anexposure of low-powered laserpuncture at reproductive acupoint of catfish brood stock is known stimulate oocyte maturation. However, the profile of oocyte resulted from laserpuncture exposure has not been known. The present study aimed to identify the profile of catfish (Clarias sp.) oocyte in post-exposure laserpuncture. A total of 48 catfishes with ages of 8-9 months was grouped into two, i.e. fishes were exposed with laserpuncture and without exposure to laserpuncture (control) with 3 replications. Laserpuncture exposure was conducted once a week until the brood stock matured. Profile of oocyte such as, the GSI value, egg diameter, total of oocytes and the gonad maturity stage were examined. The results showed that laserpuncture exposure at the catfish reproductive acupoint could trigger the oocyte development without reducing quality of the oocyte indicated by the increase of gonadosomatic index (GSI), oocyte diameter, total of oocytes and gonad maturity stage similar to control. Moreover, laserpuncture exposure accelerated the gonad maturation, three weeks faster than control.

2019 ◽  
Vol 18 (1) ◽  
pp. 9-22
Author(s):  
Tatak Dwi Cahyono ◽  
Muhammad Zairin Junior ◽  
Odang Carman

ABSTRACT The present study is a preliminary research for producing mullet fry to aquaculture. The research aimed to evaluate stimulation of gonad maturation in mullet (Mugil dussumieri) using hormones. The method used for research was completely randomized design consisting of three treatments and each individual replication was repeated three times. Two experiments were conducted separately with different treatments. First experiment used 9-14.7 cm body length of fish treated with different hormone injections i.e. 4 mg/kg 17α-methyltestosterone (MT), 0.07 mg/kg estradiol-17β (E2), and 0.5 ml/kg 0.9% physiological solution as control. Second experiment used 10-31 cm body length fish treated with 750 IU/kg chorionic gonadotropin (hCG), 0.5 ml/kg Ovaprim, and 0.9% physiological solution as control. Gonadosomatic index (GSI) value of the first experiment on day 60 showed that GSI of E2 treatment (1.31±0.94%) was higher than both MT treatment (1.00±0.51%) and control (0.54±0.20%). On the other hand, the second experiment on day 60 showed that GSI of hCG treatment (7.18±0.59%) was higher than both Ovaprim treatment (3.29±2.66%) and control (6.72±0.32%). Egg diameter frequency distribution for control in the first experiment on day 30 showed that egg size ranged from 9−144 µm. Egg diameter for E2 and MT treatments on day 60 showed that egg size ranged from 9−243 µm and were higher than control. In the second experiment, egg diameter on day 30 for control showed that egg size ranged from 9-144 µm, hCG treatment showed egg size ranged from 9−441 µm, while Ovaprim egg size ranged from 9-111 µm. Blood glucose, blood cholesterol, testosterone and estradiol hormone level in the first and second experiment showed no significant difference. The results showed that estradiol-17β and 17α-methyltestosterone induction in 9-14.7 cm body length mullet increase gonad maturity to stage II while hCG induction in 10-31 cm body length mullet increase gonad maturity to stage III. Keywords : estradiol-17β, hCG, 17α-methyltestosterone, Mugil dussumieri, Ovaprim ABSTRAK Penelitian merupakan rintisan untuk menghasilkan benih ikan belanak dalam wadah budidaya. Penelitian ini bertujuan untuk mengevaluasi pematangan gonad ikan belanak Mugil dussumieri menggunakan hormon. Penelitian dilakukan menggunakan rancangan acak lengkap yang terdiri dari tiga perlakuan dan tiga kali ulangan individu. Terdapat dua percobaan dengan perlakuan berbeda dan dilakukan secara terpisah. Percobaan pertama menggunakan ikan berukuran 9−14.7 cm dengan hormon 17α-metiltestosteron (MT) 4 mg/kg, estradiol-17β (E2) 0.07 mg/kg dan kontrol larutan fisiologis 0.9% 0.5 ml/kg. Percobaan kedua menggunakan ikan berukuran 10−31 cm dengan human chorionic gonadotropin (hCG) 750 IU/kg, Ovaprim 0.5 ml/kg dan kontrol larutan fisiologis 0.9 % 0.5 ml/kg. Nilai gonadosomatic index (GSI) percobaan pertama pada hari ke-60 menunjukkan bahwa pemberian estradiol-17β (1.31±0.94%) lebih tinggi dibandingkan 17α-metiltestosteron (1.00±0.51%) dan kontrol (0.54±0.20%). Hasil percobaan kedua pada hari ke-60 nilai GSI menunjukkan bahwa pemberian hCG (7.18±0.59%) lebih tinggi dibandingkan Ovaprim (3.29±2.66%) dan kontrol (6.72±0.32%). Sebaran frekuensi diameter telur pada percobaan pertama untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm. Sebaran frekuensi diameter telur untuk estradiol-17β dan 17α-metiltestosteron hari ke-60 menunjukkan kisaran 9−243 µm lebih banyak dibandingkan kontrol. Sebaran frekuensi diameter telur pada percobaan kedua untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm, hCG 9−441 µm sedangkan Ovaprim hanya 9−111 µm. Kadar glukosa darah, kolesterol darah, hormon testosteron dan estradiol pada percobaan pertama dan percobaan kedua menunjukkan tidak terdapat perbedaan yang nyata. Hasil penelitian menunjukkan bahwa induksi hormon estradiol-17β dan 17α-metiltestosteron pada ikan berukuran 9−14.7 cm dapat meningkatkan kematangan gonad mencapai TKG II sedangkan induksi hormon hCG pada ikan berukuran 10−31 cm dapat meningkatkan kematangan gonad mencapai TKG III. Kata kunci : estradiol-17β, hCG, 17α-metiltestosteron, Mugil dussumieri, Ovaprim


2009 ◽  
Vol 15 (1) ◽  
pp. 45-52
Author(s):  
Yunizar Ernawati ◽  
Eko Prianto ◽  
A. Ma'suf

Study on biology reproduction was expected to give basic information for management, such as for aquaculture, capture fisheries and conservation of P. polyuranodon in Musi river. Sampling were conducted on June 2006, August 2006, January 2007, and July 2007 in Musi river by using gillnet. Data analysis included length-weight relationship, sex ratio, condition factor, gonad maturity stage, Gonadosomatic Index (GSI), fecundity, and spawning type. Total number of collected P. polyuranodon was 51, consisted of 23 male and 28 female. Total length of fish was ranged in 85–511 mm. Length-weight relationship of male and female was W = 0.00002L2.8062 and W = 0.0002L2.8215 respectively. The result from t-test analysis showed that both of sexes have allometric negative growth. Sex ratio thoroughly was balance (1:1). Mean of factor condition relatively increased by the increasing of gonad maturity stage. At that time P. polyuranodon was entering spawning time. June and August was predicted as the spawning time of this species. Gonadosomatic index of female was much higher than the male. Fecundity of female was ranged between 616 and 7,059. Based on distribution of egg diameter, this species was total spawner.


Author(s):  
N. P. Sergeeva

Longterm data on the liver weight, hepatosomatic (HSI) and gonadosomatic (GSI) indices of walleye pollock, Pacific cod and saffron cod are analyzed on Eastern Kamchatka in seasonal aspect and in the course of gonad maturation. Individual values of the indices extensively vary and depend on the fish body length, season and stage of gonad maturity. The dynamics of the HSI is agree to the dynamics of the GSI during the year and depending on gonad maturity. The maximum values of the HSI are typical for walleye pollock and Pacific cod individuals with the gonads of the III stage of maturity. The maximum values of the index in saffron cod are found in mature individuals with immature gonads. The minimum values are demonstrated by the fish with the gonads at the stage of final spawning or of post-spawning recovery. During the annual reproduction cycle males and females of walleye pollock and cod realize different scenarios of spending and accumulating liver fat. Whereas the maximum HSI is observed in the fish with the gonads at the III maturity stage, the minimum values in males are typical for individuals with smelting gonads, and in females – on finishing the spawn. That can be connected to possible feeding for the individual. Liver weight and liver index can increase in males just shortly after finishing spawning, in females that is found only at the stage of post-spawning recovery.


2017 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
Nurbety Tarigan ◽  
Iman Supriatna ◽  
M. Agus Setiadi ◽  
Ridwan Affandi

The aim of this study was to examine the effect of vitamin E on gonad maturation and determining the best doses in improving gonad maturation in nilem fish. In this study used a flockof fishbrood which is never experienced spawning. There are 4 doses of vitamin E which used in this research following by 0, 125, 250, and 375 mg/kg. The results showed that fortifiedof vitamin E which were in feed significantly affected to the percentage of gonad development, gonadosomatic index (GSI), hepatosomatic index (IHS), fecundity, the diameter of eggs, and the percentage of egg maturity at P<0.05. The result showed that the best dose of vitamin E to reach gonad maturity was 375 mg kg-1 with six weeks maintenance. The percentage of gonad maturity was 100%, IGS was 11.50%, IHS was 0.34%, fecundity was 23.484 eggs/individual, the diameters of the eggs were 0.31 mm, and the percentage of mature eggs was 64.44%. These results supported the potential of feed additives such as vitamin E plays a critical role in enhancing gonad maturation in nilem fish thereby providing methods to improve fish reproduction


2013 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
. Sulistiono

<p>Tank goby (<em>Glossogobius giuris</em>) is a commonly fish found in coastal area. This study was aimed to investigate reproduction of the fish. Samples were collected from July to December 2005 from fish caught by fishermen using gill net and trap net in Ujung Pangkah Waters. Analysis was done to estimate sex ratio, gonad maturity, gonado somatic index, fecundity, and oocyte diameter. During the observation, the tank goby fish was 198 individual consisted of 112 male and 86 female fish varied 63‒230 mm in total body length. Sex ratio was around 1:1.1. First maturity gonad of male was 111‒134mm and female was 87‒110 mm total body length, respectively. According to gonad maturity stage and gonado somatic index, the fish was estimated to spawn from July to December. During July and August, the gonad maturity stage and gonado somatic index were higher indicating a lot of spawning fish during those months. Fecundity was 10,640‒150,639eggs and oocyte diameter was 49‒372 µm. Based on the oocyte distribution, the fish was estimated apartial spawner<em>.</em></p> <p>Keywords: Ujung Pangkah Waters, reproduction,tank goby,<em>Glossogobius giuris</em></p>


2019 ◽  
Vol 18 (2) ◽  
pp. 152-161
Author(s):  
Wahyu Pamungkas ◽  
Dedi Jusadi ◽  
Muhammad Zairin Junior ◽  
Mia Setiawati ◽  
Eddy Supriyono ◽  
...  

ABSTRACT This study was conducted to evaluate the profile of 17ß-estradiol (E2) and vitellogenin (Vtg) in plasma and egg diameter during gonad maturity process of striped catfish Pangasianodon hypophthalmus. Blood samples were collected from immature striped catfish, male and female with different stage of gonad maturity (stage I, II, III, and IV) to measure the concentrations of E2 and Vtg. Gonad maturity development of striped catfish was observed based on egg diameter. Result showed that E2 concentrations were the highest (843.65 pg/mL) on female with maturity stage III, the lowest on the male (26.34 pg/mL), and immature female fish (29.37 pg/mL). The protein band of Vtg was obtained on the plasma of the mature female (stage I, II, III and IV) with a molecular weight (MW) between 140−180 kDa, but it was not obtained on immature female dan male striped catfish. The highest concentration of Vtg was found in the plasma of the female fish with maturity stage III (87.34 mg/mL), then on the stage II (74.83 mg/mL), I (68.58 mg/mL), and IV (33.45 mg/mL). It showed that egg yolk formation occurred in the female mature. The average egg diameter was 0.107 ± 0.052 mm, 0.318 ± 0.086 mm, 0.864 ± 0.099 mm, and 1.041 ± 0.058 mm on the maturity stage I, II, III, and IV respectively. The increase of egg diameter along with development of gonad maturity stage indicated that egg development occurred due to the process of vitellogenesis and the addition of egg yolk on oocyte. Keywords : egg diameter, gonad maturity, striped catfish , 17ß–estradiol, vitellogenin ABSTRAK Penelitian dilakukan untuk mengevaluasi profil estradiol-17β (E2), vitelogenin (Vtg) dalam plasma dan diameter telur pada proses pematangan gonad ikan patin siam (Pangasianodon hypophthalmus). Sampel darah untuk pengukuran konsentrasi E2 dan Vtg plasma diperoleh dari ikan patin siam betina yang belum matang gonad, ikan jantan, ikan betina dengan tahap kematangan gonad yang berbeda (tahap I, II, III dan IV). Perkembangan kematangan gonad ikan patin siam diamati berdasarkan diameter telur. Hasil penelitian menunjukkan bahwa konsentrasi E2 tertinggi (843,65 pg/mL) pada ikan betina dengan kematangan tahap III, terendah pada ikan jantan (26,34 pg/mL), dan ikan betina tidak matang gonad (29,37 pg/mL). Pita protein Vtg pada sampel plasma diperoleh dari betina matang gonad (tahap I, II, III dan IV) dengan berat molekul antara 140-180 kDa, tetapi tidak diperoleh pada ikan patin siam betina yang belum dewasa dan jantan. Nilai konsentrasi tertinggi Vtg ditemukan dalam plasma darah ikan betina dengan tingkat kematangan III (87,34 mg/mL) kemudian pada tahap II (74,83 mg/mL), I (68,58 mg/mL) dan IV (33,45 mg/mL). Hal ini menunjukkan bahwa pada ikan betina dewasa terjadi proses pembentukan kuning telur (vitelogenesis). Rata-rata diameter telur adalah 0,107 ± 0,052 mm, 0,318 ± 0,086 mm, 0,864 ± 0,099 mm dan 1,041 ± 0,058 mm pada tingkat kematangan I, II, III dan IV secara berurutan. Peningkatan nilai diameter telur seiring dengan perkembangan tahap kematangan gonad menunjukkan bahwa perkembangan telur terjadi karena proses vitelogenesis dan penambahan bahan kuning telur pada oosit. Kata kunci : diameter telur, 17ß-estradiol, kematangan gonad, patin siam, vitelogenin


2021 ◽  
Vol 934 (1) ◽  
pp. 012014
Author(s):  
N Asiah ◽  
N Aryani ◽  
N Nuraini ◽  
I Lukistyowaty ◽  
Y Harjoyudanto

Abstract The Osteochilus melanopleurus (Bleeker, 1852) is one of the endogenous fish in the Siak River waters of Riau province which has high economic value. However, the fulfillment of needs still depends on the catch in nature. Efforts to overcome these problems are the availability of mature gonads. Broadly to accelerate the maturation of gonads through hormone induction until the fish are ready to be spawned to produce fish fry continuously. The purpose of this study was to analyze the effect of gonadotropin and anti-dopamine hormone induction with the trademark “Oodev” on the final gonad maturity (TKG) of the prospective broodstock of O. melanopleurus. The research was conducted from April to August 2021 in the fish rearing column and the experimental pond, Faculty of Fisheries and Marine Science. The research method was a Completely Randomized Design with 4 treatment levels and 3 replications, while the treatments were P0 (without Oodev injection), P1 (Oodev dose 0.5 mL/kg), P2 (0.7 mL/kg), and P3 (0,9 mL/kg). Measured responses: percentage of broodstock that reached TKG and egg diameter. The results of the research that have been carried out, the measurement of the diameter of the eggs produced showed that the use of the Oodev hormone at a dose of 0.7 mL/kg body weight of O.melanopleurus given every week for twelve weeks was able to stimulate the development of the gonads of O.melanopleurus. Ovaprim injection of 0.7 mL/kg body weight produced 89,566 eggs with a latency of 4 hours. oodev injection dose of 0.7 ml/kg BW or P2 treatment has succeeded in achieving a TKG of 82%.


2018 ◽  
Vol 17 (2) ◽  
pp. 227
Author(s):  
Praditha Novianingrum ◽  
Djumanto Djumanto ◽  
Murwantoko Murwantoko ◽  
Eko Setyobudi

Largehead hairtail (Trichiurus lepturus) is one commodity fish catches with high production and value in Yogyakarta. The purpose of this research is to analyse reproductive aspects of this fish in coastal area of Bantul Regency. Yogyakar-ta. Those aspects are gonadal maturity level, fecundity, and the length at the first gonadal maturity. Fish samples are taken from the catch of fisherman using gillnet with a mesh sizes of 1.75 and 2 inches in the coastal fishing port of Depok and Samas. The fish collected from January to April 2016 with at least 30 individuals every two weeks. The samples are identified to find their sex and measured to know the length, weight, and gonadal maturity level (TKG). The data analyzed include length distribution, weight, sex ratio, gonadosmoatic index, length at the first gonadal maturity (Lm), fecundity and oocyte diameter. The result shows the fish length varies between 52.8 and 86.2 cm, and weight 115 and 567.2 g. The sex ratio between male and female was 1:0.6. Gonad maturity stage II and III are found every month observation while gonad maturity stage IV found in April. Oocyte diameter in the stage of gonadal ma-turity IV was 0.35-1.02 mm, there were two distribution modus so that the spawning expected partial spawner with fec-undity of 24.601 eggs with range was 12.873-33.534 eggs. Fecundity increased as parent’s body length with equation F= 0,434L2.60. Hairtail expected reaching the length of the first gonadal maturity in 65.55 cm, higher than the average captured, namely 63.4 cm. AbstrakIkan layur (Trichiurus lepturus) merupakan salah satu komoditas hasil tangkapan ikan di Daerah Istimewa Yogyakarta dengan jumlah produksi dan nilai yang tinggi. Penelitian ini bertujuan untuk mengkaji aspek reproduksi yang meliputi: tingkat kematangan gonad, fekunditas, dan ukuran ikan kali pertama matang gonad layur di perairan pantai Kabupaten Bantul, Daerah Istimewa Yogyakarta. Sampel ikan dikumpulkan dari hasil tangkapan nelayan yang menggunakan jaring insang dengan ukuran mata jaring 1,75 dan 2 inci di Tempat Pelelangan Ikan Depok dan Samas. Pengumpulan sampel ikan dilakukan pada bulan Januari hingga April 2016 setiap dua minggu sekali minimal 30 ekor. Sampel ikan diidentifikasi jenis kelamin, diukur panjang, bobot tubuh, bobot gonad, dan tingkat kematangan gonad (TKG). Data yang dianalisis meliputi sebaran panjang, bobot, nisbah kelamin, indeks kematangan gonad, ukuran ikan kali pertama matang gonad (Lm), fekunditas, dan diameter telur. Hasil pengamatan menunjukkan kisaran panjang ikan 52,8-86,2 cm dan bobot 115-567,2 g, nisbah betina dan jantan sebesar 1 : 0,6. TKG II dan III ditemukan pada setiap bulan pengamat-an sedangkan TKG IV banyak ditemukan pada bulan April. Diameter telur ikan TKG IV berkisar 0,35-1,02 mm, terda-pat dua modus persebaran sehingga pemijahan diduga bertahap dan fekunditas sebanyak 24.601 butir dengan kisaran 12.873-33.534 butir. Fekunditas meningkat seiring panjang tubuh induk dengan persamaan F = 0,434L2,60. Ukuran ikan layur kali pertama matang gonad diperkirakan pada panjang 65,55 cm, lebih panjang daripada ukuran rata-rata tertangkap, yaitu pada 63,4 cm.


Author(s):  
Nuning Setyaningrum ◽  
Sugiharto Sugiharto ◽  
Hexa Apriliana Hidayah

<p>The fish’s reproduction status is affected by both fed compositions and vitamins intake lead to determine improvement of eggs quality as well as fish production. The presence of Ascorbic Acid (AA) in the cultivation ponds, might accelerate female’s gonad maturation and so rematuration. The research  aimed to determine: (1) Level gonad maturity of supplementation Ascorbic Acid; (2) Oocyts diameter; (3) Larvae survival rate. The research used experimental methods.The method was a completely randomized design (CRD) of 4 treatments and 4 replications.  Treatments were supplementation of AA at different dosages of 0, 600, 1200 and 1800 mg/kg fish-fed ration.The gonad maturity level was analysed descriptively and oocyte diameter and larvae survival rate data were analysed  by  ANOVA. The result showed that supplementation of AA at a dosage of 1200 mg / kg fish-fed ration accelerated the process of gonad maturity, development of oocyt diameter and larvae survival rate as well as the viability of <em>O. vittatus</em> larvae at 90 rearing days.<em>  </em>In this case, level IV gonad maturity was reached at 90 days which characterized by completed vitelogenesis process and oocyte diameter of 1.1 mm.  Thus, this study useful for aquaculture science by provide information on utilization of ascobic acid as food supplement in fish culture and also for fish farmer who wish to accelerate <em>O. vittatus</em> reproduction.</p>


2018 ◽  
Vol 17 (2) ◽  
pp. 227
Author(s):  
Praditha Novianingrum ◽  
Djumanto Djumanto ◽  
Murwantoko Murwantoko ◽  
Eko Setyobudi

Largehead hairtail (Trichiurus lepturus) is one commodity fish catches with high production and value in Yogyakarta. The purpose of this research is to analyse reproductive aspects of this fish in coastal area of Bantul Regency. Yogyakar-ta. Those aspects are gonadal maturity level, fecundity, and the length at the first gonadal maturity. Fish samples are taken from the catch of fisherman using gillnet with a mesh sizes of 1.75 and 2 inches in the coastal fishing port of Depok and Samas. The fish collected from January to April 2016 with at least 30 individuals every two weeks. The samples are identified to find their sex and measured to know the length, weight, and gonadal maturity level (TKG). The data analyzed include length distribution, weight, sex ratio, gonadosmoatic index, length at the first gonadal maturity (Lm), fecundity and oocyte diameter. The result shows the fish length varies between 52.8 and 86.2 cm, and weight 115 and 567.2 g. The sex ratio between male and female was 1:0.6. Gonad maturity stage II and III are found every month observation while gonad maturity stage IV found in April. Oocyte diameter in the stage of gonadal ma-turity IV was 0.35-1.02 mm, there were two distribution modus so that the spawning expected partial spawner with fec-undity of 24.601 eggs with range was 12.873-33.534 eggs. Fecundity increased as parent’s body length with equation F= 0,434L2.60. Hairtail expected reaching the length of the first gonadal maturity in 65.55 cm, higher than the average captured, namely 63.4 cm. AbstrakIkan layur (Trichiurus lepturus) merupakan salah satu komoditas hasil tangkapan ikan di Daerah Istimewa Yogyakarta dengan jumlah produksi dan nilai yang tinggi. Penelitian ini bertujuan untuk mengkaji aspek reproduksi yang meliputi: tingkat kematangan gonad, fekunditas, dan ukuran ikan kali pertama matang gonad layur di perairan pantai Kabupaten Bantul, Daerah Istimewa Yogyakarta. Sampel ikan dikumpulkan dari hasil tangkapan nelayan yang menggunakan jaring insang dengan ukuran mata jaring 1,75 dan 2 inci di Tempat Pelelangan Ikan Depok dan Samas. Pengumpulan sampel ikan dilakukan pada bulan Januari hingga April 2016 setiap dua minggu sekali minimal 30 ekor. Sampel ikan diidentifikasi jenis kelamin, diukur panjang, bobot tubuh, bobot gonad, dan tingkat kematangan gonad (TKG). Data yang dianalisis meliputi sebaran panjang, bobot, nisbah kelamin, indeks kematangan gonad, ukuran ikan kali pertama matang gonad (Lm), fekunditas, dan diameter telur. Hasil pengamatan menunjukkan kisaran panjang ikan 52,8-86,2 cm dan bobot 115-567,2 g, nisbah betina dan jantan sebesar 1 : 0,6. TKG II dan III ditemukan pada setiap bulan pengamat-an sedangkan TKG IV banyak ditemukan pada bulan April. Diameter telur ikan TKG IV berkisar 0,35-1,02 mm, terda-pat dua modus persebaran sehingga pemijahan diduga bertahap dan fekunditas sebanyak 24.601 butir dengan kisaran 12.873-33.534 butir. Fekunditas meningkat seiring panjang tubuh induk dengan persamaan F = 0,434L2,60. Ukuran ikan layur kali pertama matang gonad diperkirakan pada panjang 65,55 cm, lebih panjang daripada ukuran rata-rata tertangkap, yaitu pada 63,4 cm.


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