scholarly journals IDENTIFICATION OF THE COMPOUNDS OF ADATHODA VASICA BY GC MS ANALYSIS AND PROBING THE MODE OF ACTION OF THE COMPOUNDS BY INSILICO STUDY

2017 ◽  
Vol 10 (4) ◽  
pp. 105
Author(s):  
Sharly Elgal ◽  
Jasmine R
Keyword(s):  
Antibacterial Activity ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Respiratory Infections ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Respiratory Pathogens ◽  
Ms Analysis ◽  
Broth Dilution

Objective: The present study was aimed to investigate the promising compound of ethanol extracts of Adathoda vasica against respiratory isolates by GC-MS analysis and Insilico studies.Methods: The leaves of the chosen plant was extracted with ethanol, evaporated in vaccum and stored for further study. The ethanol extract was analyzed for antibacterial activity against respiratory pathogens. To determine the dosage of the extracts against target respiratory isolates, minimal inhibitory concentration has been determined by broth dilution method. The extract was screened to identify the promising compounds by GC-MS and Docking was performed using Schrodinger software to determine the effective of these compounds with target proteins against respiratory infections.Results: The ethanol extract of Adathoda vasica showed promising antibacterial activity by agar diffusion method, to determine the dosage minimal inhibitory concentration has been determined by broth dilution method (MIC=3.125mg),were highly susceptible to the extract. The ethanol extract subjected to the GC-MS analysis, based on the percentage of peak area, the 3 promising compounds were chosen for docking analysis. Insilico studies had showed effective binding with target protein 2WYP (Sialic acid binding protein) and 1X7Y(α-Ketoacid dehydrogenase).Conclusion: The above study concludes that plant Adathoda vasica can be used in Ayurvedic treatment and has no side effect to cure both lower and respiratory infections. Thus we can also extend our study to drug formationKeywords: Adathoda vasica, GC-MS, 2-4(but-2-yl)phenyl)propanoicacid, N,N-dimethylglycine n-Hexanedecanoic acid.

scholarly journals Skrining Aktivitas Antibakteri Ekstrak Etanol Daun Terhadap Salmonella Typhi Resisten Kloramfenikol

2017 ◽  
Vol 2 (02) ◽  
pp. 66
Author(s):  
Ika Trisharyanti
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Dilution Method ◽  
Tea Leaves ◽  
Disk Diffusion Method ◽  
Phaleria Macrocarpa

<em><span lang="EN-US">Typhoid fever is an infectious disease caused by Salmonella typhi, antibiotic chloramphenicol can be use to treatment, but S. typhi resistant with antibiotic choramphenicol so need to alternative treatment. The purpose of this study was to determine the antibacterial activity of ethanol extract of ten leaves against Salmonella typhi, and knowing the compounds contained in extracts of leaves that have the best antibacterial activity. The leaves was extracted with  ethanol 96% by maceration method. Screening antibacterial activity used disk diffusion method with 10% extract concentration. The best antibacterial activity was determined Minimum Inhibitory Concentration and Minimum Bactericidal Concentration used liquid dilution method, identification test of compounds with thin-layer chromatograpy (TLC), and bioautografi test. There were six extracts had antibacterial activity against S. Typhi, Phaleria macrocarpa leaves, Acalypha siamensis leaves, cherry leaves, clove leaves, tea leaves, and Eugenia polyantha leaves. Minimum Bactericidal Concentration from clove leaf extract was 2,5%. The results of identification by TLC compounds were alkaloids, phenolics, flavonoids, terpenoids, triterpenoids, and saponins. Based on bioautografi test,  phenolic can inhibit the growth of Salmonella typhi.</span></em>

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI KRIM EKSTRAK ETANOL HERBA SELEDRI (Apium graveolens L.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 226
Author(s):  
Garry Clements ◽  
Paulina V. Y. Yamlean ◽  
Widya Astuty Lolo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Apium Graveolens ◽  
Dilution Method ◽  
Herb Extract

ABSTRACTCelery herbs (Apium graveolens L.) have content flavonoid, saponin and tannin. Flavonoids have anti-inflammatory properties, antioxidants and antibacterials. This study aims to determine whether the ethanol extract of celery herbs can be formulated into cream preparations, test the quality of preparations at concentrations of 5%, 10% and 15%. These methods of research are experimental laboratories by making the supply of self-sustaining herbal celery ethanol extract cream and testing the quality of the cream preparation,  as well as testing of antibacterial activity was carried out using the liquid dilution method and measuring value of  Minimum Inhibitory Concentration  and Minimum Bactericidal Concentration using a UV-Vis Spectrophotometer. This research shows that celery herbs ethanol extract can be formulated into cream preparations, fulfilling quality test of organoleptic, scatter power, adhesion and cycling test, but that doesn’t meet the requirements for homogeneity tests and pH tests and has antibacterial activity which not only inhibits but kills bacteria in preparations of 5% and 15%Keywords: Cream Celery herb extract, Antibacterial, Staphylococcus aureus, UV-Vis spectrophotometer ABSTRAK Herba Seledri (Apium graveolens L.) memiliki kandungan flavonoid, saponin dan tannin. Flavonoid memiliki khasiat sebagai antiinflamasi, antioksidan dan antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, menguji mutu sediaan pada konsentrasi 5%, 10% dan 15%. Metode penelitian ini experimental laboratorium dengan membuat Sediaan Krim Ekstrak Etanol Herba Seledri dan menguji mutu sediaan krim, serta   menguji aktivitas antibakteri menggunakan metode dilusi cair dan mengukur nilai KBM dan KHM menggunakan Spektrofotometer UV-Vis. Hasil penelitian menujukkan bahwa ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, memenuhi uji mutu sediaan organoleptik, daya, sebar, daya hambat dan stabilitas  namun tidak memenuhi persyaratan untuk uji homogenitas dan uji pH serta mempunyai aktivitas antibakteri yang membunuh bakteri pada sediaan berkonsentrasi 5% dan 15%.Kata Kunci : Krim Ekstrak herba Seledri, Antibakteri, Staphylococcus aureus, Spektrofotometer UV-Vis

scholarly journals Uji aktivitas antibakteri ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Vivi Permata Sari ◽  
Wiwin Retnowati ◽  
Yuani Setiawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimal Inhibitory Concentration ◽  
Leucaena Leucocephala ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Seed Extract ◽  
Dilution Method ◽  
Minimal Bactericidal Concentration ◽  
Vancomycin Resistant

 Abstract. Staphylococcus aureus merupakan flora normal kulit manusia yang bisa menyebabkan infeksi ketika sistem imun tubuh manusia sedang lemah. Pengobatan Staphylococcus aureus tergolong sulit karena Staphylococcus aureus mudah resisten terhadap beberapa antibiotik sehingga dikenal istilah Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-intermediate Staphylococcus aureus (VISA), dan Vancomycin-resistant Staphylococcus aureus (VRSA) yang menunjukkan resistensi tersebut. Pertumbuhan Staphylococcus aureus dapat dihambat ekstrak daun petai cina (Leucaena leucocephala). Tanaman petai cina (Leucaena leucocephala) digunakan masyarakat Indonesia sebagai obat tradisional untuk menyembuhkan luka, mengobati cacingan, dan mengobati jerawat. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak biji petai cina (Leucaena leucocephala) dalam menghambat pertumbuhan Staphylococcus aureus.Penelitian ini merupakan eksperimental laboratorium dengan metode dilusi sebanyak 6 replikasi. Setiap replikasi menggunakan konsentrasi ekstrak biji petai cina (Leucaena leucocephala) sebesar 3,125%, 6,25%, 12,5%, 25%, 50%, dan 100% untuk mencari konsentrasi hambat minimal dan konsentrasi bunuh minimal terhadap Staphylococcus aureus.Hasil penelitian tidak dapat menentukan konsentrasi hambat minimal (KHM) ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus karena warna ekstrak yang gelap. Sedangkan konsentrasi bunuh minimal (KBM) ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus ditemukan pada konsentrasi 50%, yang menunjukkan bahwa konsentrasi 50% adalah konsentrasi terkecil dimana tidak terdapat pertumbuhan bakteri Staphylococcus aureus.Sebagai simpulan, ekstrak biji petai cina (Leucaena leucocephala) memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Kata kunci: Staphylococcus aureus, Leucaena leucocephala, metode dilusi  Abstract. Staphylococcus aureus is a normal human skin flora that can cause infection when human immune system is weak. Staphylococcus aureus treatment is difficult because Staphylococcus aureus is easily resistant to some antibiotics so several terms such as Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-intermediate Staphylococcus aureus (VISA), and Vancomycin-resistant Staphylococcus aureus (VRSA) are known to indicate the resistance. The growth of Staphylococcus aureus can be inhibited by Leucaena leucocephala leaf extract. Leucaena leucocephala is used by Indonesian people as traditional medicine to heal wounds, treat worm infection, and treat acne. The objective of this study was to identify the antibacterial activity of Leucaena leucocephala seed extract against Staphylococcus aureus.This was a laboratory experimental study using dilution method as many as five replications. Each replication used Leucaena leucocephala seed extract in concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100% to determine the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) against Staphylococcus aureus. The results were not able to determine the minimal inhibitory concentration (MIC) of Leucaena leucocephala seed extract against Staphylococcus aureus due to dark color of the extract. While the minimal bactericidal concentration (MBC) of Leucaena leucocephala seed extract against Staphylococcus aureus was 50%. This showed that the concentration of 50% was the lowest concentration where there was no growth of the bacteria Staphylococcus aureus.In conclusion, the extract of Leucaena leucocephala seed has antibacterial activity against Staphylococcus aureus. Keywords: Staphylococcus aureus, Leucaena leucocephala, dilution method

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli

2016 ◽  
Vol 16 (1) ◽  
pp. 51-56
Author(s):  
Hilda Maysarah ◽  
Rika Apriani ◽  
Misrahanum Misrahanum
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Guava Leaf ◽  
Mic Value

An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli.  MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.

scholarly journals Preparation, characterization and antibacterial effect of 2-methoxy-6-(5-H/Me/Cl/No2-1H-benzimidazol-2-yl)phenols and some transition metal complexes

2009 ◽  
Vol 74 (5) ◽  
pp. 537-548 ◽  
Author(s):  
Aydin Tavman ◽  
Serkan Ikiz ◽  
Funda Bagcigil ◽  
Yakut Özgür ◽  
A.K. Seyyal
Keyword(s):  
Antibacterial Activity ◽  
Transition Metal Complexes ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disk Diffusion Method ◽  
1H And 13C Nmr ◽  
Gram Positive Bacteria ◽  
Ft Ir

2-Methoxy-6-(5-H/methyl/chloro/nitro-1H-benzimidazol-2-yl)phenols (HLx; x = 1-4, respectively) ligands and HL1 complexes with Fe(NO3)3, Cu(NO3)2, AgNO3 and Zn(NO3)2 were synthesized and characterized. The structures of the compounds were confirmed based on elemental analysis, molar conductivity, magnetic moment, FT-IR, 1H- and 13C-NMR. The antibacterial activity and minimum inhibitory concentration (MIC) of the free ligands, their hydrochloride salts and the complexes were evaluated using the disk diffusion method in dimethyl sulfoxide (DMSO) and the dilution method, respectively, against 9 bacteria. HL1 and HL3, as well as the Cu(II) and Zn(II) complexes, showed antibacterial activity against Gram-positive bacteria.

scholarly journals Aktivitas Antibakteri Ekstrak Metanol Bunga Teratai (Nymphaea pubescens Willd)

2020 ◽  
Vol 7 (2) ◽  
pp. 75
Author(s):  
Amalia Khairunnisa ◽  
Nashrul Wathan ◽  
Mia Fitriana ◽  
Fadlilaturrahmah Fadlilaturrahmah ◽  
Nisriyati Fiddina
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Test Methods ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test

Nymphaea pubescens Willd telah diketahui mempunyai efek antibakteri, terutama pada biji dan daunnya. Tetapi sampai saat ini bagian bunga dari tanaman tersebut belum dilakukan pengujian aktivitas antibakteri. Tujuan dari penelitian ini untuk melakukan skrining fitokimia, uji aktivitas antibakteri dan penentuan konsentrasi hambat minimum (KHM) dari ekstrak metanol bunga N. pubescens terhadap S. aureus dan E. coli. Proses ekstraksi bunga N. pubescens dimaserasi menggunakan pelarut metanol dengan perbandingan 1: 4 b/v. Metode pengujian yang digunakan ada dua yaitu metode difusi untuk pengujian aktivitas antibakteri dan metode dilusi untuk pengukuran konsentrasi hambat minimum (KHM). Hasil skrining fitokimia didapatkan bahwa ekstrak metanol bunga N. pubescens mengandung senyawa golongan fenolik, saponin dan flavonoid. Hasil pengujian aktivitas antibakteri menunjukkan bahwa ekstrak metanol bunga N.pubescens mampu menghambat S. aureus (diameter hambat 10 ± 0,29 mm) dan E.coli (diameter hambat 10,2 ± 0,50 mm). Konsentrasi hambat minimum dari ekstrak metanol bunga N. pubescens terhadap S.aureus sebesar 12,5% dan terhadap E.coli sebesar 25%. Dapat disimpulkan bahwa ekstrak metanol bunga N. pubescens memiliki aktivitas sebagai antibakteri. Nymphaea pubescens Willd has known to have antibacterial effects, especially on the seeds and leaves. However, until now the flower of the plant has not been tested for antibacterial activity. The purpose of this study was to perform phytochemical screening, antibacterial activity test and determine the minimum inhibitory concentration (MIC) of the methanol extract of N. pubescens flowers against S. aureus and E. coli. The process of extracting N. pubescens flowers is macerated using methanol as a solvent with a ratio of 1: 4 w / v. There are two test methods used, namely the diffusion method for testing antibacterial activity and the dilution method for measuring the minimum inhibitory concentration (MIC). The results of phytochemical screening showed that the methanol extract of N. pubescens flowers contained phenolic compounds, saponins, and flavonoids. The results of the antibacterial activity test showed that the methanol extract of N.pubescens flowers was able to inhibit S. aureus (inhibition diameter 10 ± 0.29 mm) and E. coli (inhibitory diameter 10.2 ± 0.50 mm). The minimum inhibitory concentration of the methanol extract of N. pubescens flowers against S.aureus was 12.5% and against E. coli was 25%. It can be concluded that the methanol extract of N. pubescens flowers has antibacterial activityKeywords: Nymphaea pubescens, lotus flower, methanol extract, antibacterial

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MAHKOTA DEWA (Phaleria macrocarpa) TERHADAP STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 9 (02) ◽  
pp. 134-139
Author(s):  
Yunilda Rosa ◽  
Masnir Alwi ◽  
Monica Julianti
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dilution Method ◽  
Phaleria Macrocarpa

Phaleria macrocarpa is one of the medicinal plants that many people use as a traditional medicinal which has many benefits, one of which is as a medicine for skin diseases. The content of flavonoid compounds in Phaleria macrocarpa believed to have potential as an antibacterial. The purpose of this study was to know the antibacterial activity of the ethanol extract of the Phaleria macrocarpa to inhibized Staphylococcus aureus bacteria. This research is an experimental study carried out by the liquid dilution method (KHM)and disk diffusion (KBM) of the extract of the Phaleria macrocarpa is divided into 6 concentrations namely 5%, 10% and 15% (KHM) and 40%, 50% and 60% (KBM). The positive control used is clindamycin and the negative control is aquadest. Data analysis uses univariate. Based on observations of the minimum inhibitory concentration of the extract of the fruit of the Phaleria macrocarpa which is at of 15%, while the minimum killed concentration is formed at of 40% with an average of 6.36 mm, and an average of zones formed on the positive control of 9.53 mm. From the results of the study, it was concluded that the extract of the fruit of the Phaleria macrocarpa can inhibit and kill the growth of Staphylococcus aureus bacteria.

scholarly journals Comparative analysis of the chemical composition and antimicrobal activities of some of Lamiaceae family species and Eucaliptus (Eucaliptus globules M)

2014 ◽  
pp. 201-213
Author(s):  
Danijela Pecarski ◽  
Zorica Knezevic-Jugovic ◽  
Suzana Dimitrijevic-Brankovic ◽  
Katarina Mihajilovski ◽  
Slobodan Jankovic
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Aromatic Hydrocarbons ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Diffusion Method ◽  
Main Components ◽  
Oil Gas ◽  
Broth Dilution

The aim of this study was to evaluate and compare the antimicrobial activity of four essential oils that belong to Lamiaceae family (sage, oregano, thyme) and eucalyptus oil. Gas chromatography coupled with mass spectrometry (GC-MS) revealed that the highest percent of essential oils 98.93% include three classes of compounds - monoterpene hydrocarbons, aromatic hydrocarbons and oxidized monoterpenes. The main components are oxidized monoterpenes: carvacrol (59.03%); thymol (36.12%), eucalyptol (20.66%), hydrocarbon monoterpenes: limonene (30.96%) and ?-pinene (12.21%) and aromatic monoterpene, p-cymene (22.25%) All essential oils showed great potential of antimicrobial activity against several bacteria and yeast C. albicans, using the agar diffusion method with wells. Minimum inhibitory concentration (MIC) for the essential oils has been determined by the broth dilution method and valued in the range from 1 to 5 ?L/m, depending on the essential oil and bacteria tested, and up to 100 ?L/ml for C. albicans. The essential oils of Lamiaceae family exhibited a strong antibacterial activity for tested microorganisms, while the essential oils of thyme were especially recognized.

scholarly journals Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

2019 ◽  
Author(s):  
kindu geta ◽  
Mulugeta Kibret
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Active Components ◽  
Microbial Infections ◽  
Ethanol Extracts ◽  
Broth Dilution

Abstract Medicinal plants offer a major and accessible source of health care to people living in developing countries. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Acanthus sennii is one of the medicinal plants used traditionally for treatment of different infectious diseases in Ethiopian. Therefore, this research was carried out to evaluate antibacterial activity of Acanthus sennii against pathogenic bacteria. The antimicrobial activity tests of the plant extracts against pathogenic bacteria were carried out by the agar well diffusion method. Broth dilution and streak plate method were used to determine MIC and MBC of extracts respectively. The results revealed that ethanol extracts of leaves showed the highest antibacterial activity against standard strains of S.aureus with inhibition zone of 14±0.58mm and 17±0.67mm at 50mg/ml and at 25mg/ml respectively. Ethanol extracts of buds showed the highest antibacterial activity against standard strains of S.aureus with inhibition zone of 25.67±0.67mm at 100mg/ml. The lowest mean MIC value (2.6±0.52 mg/ml) was recorded with ethanol extract of leaves against standard strains and clinical isolates of S. aureus. The lowest mean MBC value (4.2±1.04 mg/ml) was recorded with ethanol extract of leaves against standard strains of S. aureu. Acanthus sennii could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant. Key words: Acanthus sennii, Antibacterial activity, MIC, MBC, pathogenic bacteria

Investigation for New Inhibitors of UDP-N-Acetylglucosamine Enolpyruvyl Transferase (MurA) by Virtual Screening with Antibacterial Assessment

2020 ◽  
Vol 16 ◽  
Author(s):  
Ilham Boulhissa ◽  
Abdelouahab Chikhi ◽  
Abderrahmane Bensegueni ◽  
Mohammad Ahmad Ghattas ◽  
El Hassen Mokrani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Virtual Screening ◽  
Pathogenic Bacteria ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Dilution Method ◽  
Chemical Library ◽  
Broth Dilution

Background: View to its interesting role in the peptidoglycan biosynthesis pathway the enzyme UDP-N- acetylglucosamine enolpyruvyl transferase is an attractive target to develop new antibacterial agents, it catalyzes the first key step of this pathway and its inhibition leads to the bacterial cell death. Fosfomycin is known as the natural inhibitor of MurA. Objective: Call new inhibitors of MurA by virtual screening of different chemical compounds libraries, and test the best scored “virtual hits” against three pathogenic bacteria: Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. Methods: A Virtual screening of the structural analogues of fosfomycin downloaded from PubChem database was performed on one side and of the French National Chemical Library as well as using ZINC database to identify new structures different from fosfomycin on the other, FlexX was the software used for this study. The antibacterial testing was divided into methods: disk diffusion and broth dilution. Results: A set of virtual hits was found with better energy score than that of fosfomycin, seven between them were tested in vitro. Therefore, disk diffusion method explored four compounds exhibited antibacterial activity: CID-21680357 (fosfomycin analogue), AB-00005001, ZINC04658565, and ZINC901335. The testing was continued by broth dilution method for both compounds CID-21680357 and ZINC901335 to determine their minimum inhibitory concentrations, and ZINC901335 had the best value with 457µg/ml against Staphylococcus aureus. Conclusion: Four compounds were found and proven in silico and in vitro to have antibacterial activity: CID-21680357, AB-00005001, ZINC04658565, and ZINC901335.

Sign in / Sign up

Export Citation Format

Share Document