scholarly journals EVALUATION AND CHARACTERIZATION OF BIOACTIVE COMPOUND OF HAMELIA PATENS PLANT WITH THE GC-MS SPECTROSCOPY

2018 ◽  
Vol 11 (11) ◽  
pp. 232
Author(s):  
Jafra Bano ◽  
Swapna Santra ◽  
Ekta Menghani
Keyword(s):  
Bioactive Compound ◽  
Methanol Extracts ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Ms Analysis ◽  
Novel Drugs ◽  
Wide Range ◽  
Phytochemical Compounds ◽  
Bioactive Fraction

Objective: The aim of this study is to evaluate the bioactive compound present in the leaves, root, flower, and stem by gas chromatography (GC) analysis. Novel drugs are isolated from plants against human diseases.Methods: The plant parts (leaves, stem, flower, and root) were collected, washed, shade dried, and powdered, and the methanol extracts of all plant parts were prepared by Soxhlet reflux method. The methanolic extracts were analyzed for the identification of phytochemical compounds present in the Hamelia patens plant parts using GC-mass spectrometry (MS) matched by the National Institute of Standards And Technology-11 library and Willey 8 library.Results: The bioactive fraction on GC-MS analysis revealed a chromatogram showing highest peaks. Methanol extracts of H. patens leaf are found to possess a wide range of saturated and unsaturated aliphatic hydrocarbons and their isomers such as 2,3-dihydro-3,5-dihydroxy-6-methyl- 4H-pyran (1.77%), 1,3-propanediol, 2-ethyl-2-(hydroxymethyl) (3.06%), mome inositol (18.22%), pentadecanoic acid (1.66%), and squalene (11.47%). GC-MS analysis revealed chromatogram, showing that the highest peaks of H. patens stem are methyl salicylate (3.41%), 2-amino-9-(3,4- dihydroxy-5- hydroxymethyl) (9.53%), mome inositol (63.73%), and squalene (1.07%).Conclusion: Isolation of such bioactives and their use as therapeutic target is the prime motto of our research to isolate novel bioactive that can used as potential therapeutic agents.

scholarly journals BIOCHEMICAL CHARACTERIZATION OF Ferula rutbaensis MEDICINAL PLANT IN IRAQI WESTERN DESERT

2020 ◽  
Vol 17 (36) ◽  
pp. 831-844
Author(s):  
Kutayba Farhan DAWOOD ◽  
Ayoob Obaid ALFALAHI ◽  
Shamil Ismail NEAMAH ◽  
Omar Mahmood DHANNOON
Keyword(s):  
Fatty Acids ◽  
Bioactive Compounds ◽  
Unsaturated Fatty Acids ◽  
Biochemical Characterization ◽  
Western Desert ◽  
Morphological Identification ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Wide Range

Plants used in folk medicine not only represent rich sources for therapeutic materials, but it also plays a crucial role in developing completely or partially novel synthesized drugs. Mharut plant (Ferula rutbaensis) is an integral part of Bedouin therapeutic practices in the western desert of Anbar province-Iraq. Still, to date, this is the first study describing its phytochemical constituents. The plant was growing near the Iraq-Saudi Arabia borders and adapted to a wide range of soils. Traditionally, F. rutbaensis has been widely used to treat acne, stomach and bowel disorders, food poisoning and respiratory problems. Fresh plant samples were collected and morphologically characterized. Likewise, the ITS-based DNA barcoding technique was efficiently used to approve the morphological identification of F. rutbaensis. The GC-MS spectrum was adopted in the phytochemical characterization of aqueous and methanol extracts of fresh and dry plant parts. The aqueous extract of dry roots was the richest source for bioactive compounds than fresh or methanolic extracts of either fresh or dry plant parts. In general, the detected phytochemicals falling into fatty acids, terpenes, hydrocarbon alkanes, and esters. Notably, fatty acids in Oleic and Palmitic acids were the two most abundant bioactive compounds in both aqueous and methanolic extracts of plant fresh and dry roots. The detected unsaturated fatty acids and/or other bioactive components are laying behind the therapeutic properties of F. rutbaensis that can be useful ingredients to prepare Mharut-based cosmetics such as medical soaps, body lotions, skin conditioners and sunscreens. Additionally, some other components were found to have anti-inflammatory, antioxidants, and antimicrobial properties. Further investigations will be necessary to confirm the antimicrobial activity of F. rutbaensis extracts.

scholarly journals Studies on the Phytochemical Compounds in the Ethanolic Leaf Extract (ELE), Ethanolic Bark Extract (EBE) and Ethanolic Root Extract (ERE) of Bridelia ferruginea Benth (Euphorbiaceae)

2019 ◽  
pp. 1-8 ◽  
Author(s):  
Abdullahi Attah Alfa ◽  
Orukotan Abimbola Ayodeji ◽  
Goji Anthony Donatus Teru ◽  
Kokori Bajeh Tijani
Keyword(s):  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Bioactive Compound ◽  
Fats And Oils ◽  
Bark Extract ◽  
Root Extract ◽  
Suction Pump ◽  
Plant Parts ◽  
Bridelia Ferruginea ◽  
Phytochemical Compounds

The phytochemical compounds of Bridelia ferruginea plant parts was carried out using qualitative method to determine the bioactive compounds present in the plant leave, stem bark and root extracts. The samples was weighed, of which 100 g each of the powder were extracted in solvents (ethanol) 1000 ml macerated and stand for 72 hours. The solvents contained in the maceration bottle was decanted and filtered using a filter paper, the filtration was aided using a suction pump. The filtrate was concentrated using a rotary evaporator and then transferred into thermostatic water cabinet (Temperature was set at 45oC), allowed to dry completely. The plant parts extracts were separately kept in a screw capped bottle for further research. The bioactive compound in the plant parts were detected. The result revealed that Carbohydrates, Saponins, Flavonoids, Tannins, Cardiac Glycosides, fats and oils were present. Alkaloid present in Dragendoff’s test in all plant parts extract but absent in Mayer’s test in only leaf extract. Terpenoids/Steroids present in Liebermann-Burchard’s test in all plant parts extract but absent in Salkowski’s test in only leaf extract. Anthraquinones were absent in all plant parts extracts using Bontrager’s test. Therefore, the presence of these phyto-pharmacological compounds is an indicative that the plant is medicinal and it can be used for the treatment of bacterial and other microbial infections. Further study can be done to separate the individual metabolites to test their antimicrobial activity against some pathogenic bacteria like bacterial meningitis, tuberculosis and syphilis to determine their potency.

scholarly journals Oral acute toxicity and antimalarial potentials of aqueous and methanolic extracts of roots, leaves and stem of Dictyandra arborescens (Welw.) on Plasmodium berghei infected mice

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Uchechi E. Enenebeaku ◽  
Nnamdi C. Ukwandu ◽  
Ifeyinwa C. Mgbemena ◽  
Harriet C. Nwigwe ◽  
Conrad K. Enenebeaku ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Scientific Evidence ◽  
Negative Control ◽  
Methanol Extracts ◽  
Methanolic Extracts ◽  
Ms Analysis ◽  
Significant Difference ◽  
Oral Acute Toxicity

Abstract Background Malaria is one of the tropical diseases of universal concern particularly with continuous appearance of resistant strains of P.falciparum. This calls for continous screening of traditional plants such that new and effective antimalarial agents will be developed. This study therefore explored the oral acute toxicity and antimalarial potentials of aqueous and methanolic extracts of roots, leaves and stem of Dictyandra arborescens on Plasmodium berghei infected mice. Results No mortality was recorded in any of the experimental animal groups even at the highest tested dose (5000 mg/kg b.wt) of the extract after monitoring them for 4hrs and subsequently for 7 days. Out of the six extracts, methanolic extracts of the roots and leaves exhibited more antimalarial activity than others. A significant difference (P < 0.05) was statistically observed in the parasite count of groups that received methanol extracts of roots and leaves of D. arborescens. This observation was made when these two extracts were compared with other groups as well as the negative control. However, activity of the standard antimalarial drug (artesunate) was higher (p˂0.05) than those of the extracts. Phytochemicals such as tannins, alkaloids, saponins, terpenoids, flavonoids etc. were present in the extracts in varying quantities. GC–MS analysis of methanol extract of the root of this plant showed different chemical compounds. Conclusion Administration of aqueous and methanol extracts of roots, leaves and stem of D. arborescens in mice is not harmful at any dose less than or equal to 5000 mg/kg. Methanol extracts exhibited more antimalarial activity than aqueous extracts suggesting that antimalarial activity of the plant parts could be affected by the solvent used for extraction and antimalarial activity may be more in a particular part of a plant. The presence of different bioactive compounds identified in phytochemical and GC–MS analysis could be the fundamental scientific evidence for the antimalarial activity exhibited by this plant especially in the root.

scholarly journals DETERMINATION OF VOLATILE BIOACTIVE COMPOUNDS FROM EXTRACTS OF BAEL (AEGLE MARMELOS) PLANT PARTS AND THEIR COMPARATIVE ANALYSIS

2018 ◽  
Vol 11 (3) ◽  
pp. 393
Author(s):  
Nidhi Sharma ◽  
Widhi Dubey
Keyword(s):  
Bioactive Compounds ◽  
Dibutyl Phthalate ◽  
Methanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Aegle Marmelos ◽  
Fruit Peel ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Ms Analysis

 Objective: The main objective of this study is to determine the bioactive compounds from the extracts of wildly growing Aegle marmelos plant parts.Methods: A. marmelos root, stem, leaves, bark, fruit peel, and pulp were screened for the presence/absence of phytochemicals. Bioactive compounds in all the plant parts were analyzed by gas chromatography–mass spectrometry (GC/MS) analysis. For evaluation of bioactive compounds first, the column chromatography was done using various solvents and found that the methanolic extracts gave better elution and separation of compounds and hence used further for GC/MS analysis.Result: GC/MS analysis revealed chromatograms of methanol extract of A. marmelos plant parts, and all the plant parts were found to have a number of phytochemicals. Some compounds, namely, benzene, nitro-, benzenepropanoic acid, 3, 5-bis (1, 1-dimethylethyl)-4-hydroxy-, methyl ester, and tetradecene were found in all parts with a varying percentage. Phenol only found in the fruit of the plant with more percentage in fruit peel (4.38%) than in fruit pulp (0.58%). Dibutyl phthalate is the major compound found in Aegle root (10.43%), fruit peel (34.56%), and pulp (13.18%). Other important compounds such as coumarin (2H-1-Benzopyran-2-one, 7-[(3,7-dimethyl-2,6-octadienyl)oxy]-, (E)-), skimmianine (Furo[2,3-b] quinoline, 4,7,8-trimethoxy-), and cyclobarbital were found in plant root.Conclusion: After the GC/MS analysis, it was concluded that all the parts of this wildly growing plant contain a significant amount of pharmaceutically important compounds.

scholarly journals Antibacterial Activity of Adansonia digitata L.: A Globally Endangered Tree

2018 ◽  
Vol 9 (11) ◽  
Author(s):  
Samatha T. ◽  
Shama N. ◽  
Chandrakala G. ◽  
Thirupathi K. ◽  
Rama Swamy N.
Keyword(s):  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Pathogenic Bacteria ◽  
Bacterial Strains ◽  
Adansonia Digitata ◽  
Endangered Tree ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Wide Range ◽  
Fruit Wall

The tree species of Adansonia digitata L. possesses wide range of medicinal properties effective against various infectious diseases. The present work has been under taken to study the antibacterial activity of different extracts of A. digitata against pathogenic bacteria. Antibacterial activity of methanolic extracts of leaf, flower and fruit wall extracts of A. digitata has been studied to find out their activity against pathogenic bacteria viz., Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Staphylococcus aureus and Enterobacter aerogenus. The activity of these extracts of different plant parts against both positive as well as negative pathogenic bacterial strains was screened through well diffusion technique by using minimum inhibitory concentration (MIC) method. According to our observations, all the extracts of A. digitata exhibited antibacterial activity against all the pathogenic bacteria under study except the fruit wall extracts which didn’t show any inhibition against K. pneumonia. Thus, results provided evidence that the species A. digitata can be used as a potential source of antibacterial agent in the treatment of various infectious diseases.

HPLC-ESI-MS Characterization of Certain Polyphenolic Compounds of Carica papaya L. Fruit Extracts and Evaluation of Their Potential Against Murine Schistosomiasis mansoni

Drug Research ◽  
2018 ◽  
Vol 68 (09) ◽  
pp. 521-528 ◽  
Author(s):  
Ezzat Abdel-Lateef ◽  
Ibrahim Rabia ◽  
Mortada El-Sayed ◽  
El-Sayed Abdel-Hameed
Keyword(s):  
Worm Burden ◽  
Carica Papaya ◽  
Methanolic Extract ◽  
Polyphenolic Compounds ◽  
Infected Group ◽  
Esi Ms ◽  
Methanolic Extracts ◽  
Ms Analysis

AbstractThe in vivo antischistosomal activities of Carica papaya L. extracts were evaluated and the characterization of the active secondary metabolites of the defatted methanolic extract was performed using HPLC-ESI-MS. The plant fruit powders were extracted with 85% methanol and fractionated using organic solvents. The in vivo antischistosomal effects of the methanolic extracts and its fractions, as well as the assessment of the relationship between the antischistosomal activity of these plant extracts and oxidative stress, was determined. In addition, the defatted methanolic extract was characterized by HPLC-ESI-MS analysis. The number of worms, ova, and the Oogram pattern displayed typical Schistosoma mansoni pathology 8 weeks after infection in mice. Treatment of the infected group with the defatted methanolic extracts significantly decreased worm burden, immature ova and mature ova, while increasing the percentage of dead ova in vivo. The butanol fraction was the most effective fraction reducing worm burden by 77%, ova count in the intestine by 76% and in the liver by 80%, and significantly decreased immature and mature ova (P<0.001) compared to the infected group. Additionally, the defatted methanolic extracts improved the reduced glutathione and malondialdehyde levels in hepatic tissues in the treated groups compared to the infected group. The HPLC-ESI-MS analysis of the Carica papaya defatted methanolic extract revealed the presence of several polyphenolic compounds. Carica papaya fruit extracts are rich with phenolic acids and flavonoids and show a significant effect against S. mansoni infections which may be used alternative to PZQ as anti-schistosomal drug against schistosomiasis.

scholarly journals Isolation and Characterization of Bioactive Anti-inflammatory Flavonoids from Arisaema tortuosum Tubers

2020 ◽  
Vol 32 (12) ◽  
pp. 3093-3096
Author(s):  
Priyanka Chakraborty ◽  
Kamalika Mazumder ◽  
Nripendra Nath Bala ◽  
Sudipta Das
Keyword(s):  
13C Nmr ◽  
Methanolic Extract ◽  
Bioactive Compound ◽  
Inflammatory Activity ◽  
Methanolic Extracts ◽  
Isolation And Characterization ◽  
Ft Ir ◽  
Anti Inflammatory

Present study was focused on the isolation and characterization of the anti-inflammatory compound(s) from the methanolic extracts of Arisaema tortuosum tuber by column chromatography, TLC, FT-IR, 1H, 13C NMR and HR-MS. Among all the fractions obtained A. tortuosum fraction 3 (PC-1) and fraction 5 (PC-2) showed significant in vivo anti-inflammatory activity. This study endorsed that the isolated bioactive compound PC-2 showed better anti-inflammatory activity at a dose of 25 mg/Kg BW. The results indicate that PC-2 and PC-1 methanolic extract of A. tortuosum tuber may be competing for the tent in the therapy of inflammatory ailments.

scholarly journals GC-MS analysis of bioactive compounds in the plant parts of methanolic extracts of Momordica cymbalaria Fenzl

2021 ◽  
Vol 9 (3) ◽  
pp. 209-218
Author(s):  
Chaitanya Gopu ◽  
Pavani Chirumamilla ◽  
Sunitha Bai Daravath ◽  
Suvarchala Vankudoth ◽  
Shasthree Taduri
Keyword(s):  
Bioactive Compounds ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Ms Analysis

Characterization of Different Types of Biomass Wastes Using Thermogravimetric and ICP-MS Analyses

2020 ◽  
Vol 70 (12) ◽  
pp. 4594-4600
Keyword(s):  
Thermogravimetric Analysis ◽  
Food Industry ◽  
Wood Waste ◽  
Biomass Ash ◽  
Minor Elements ◽  
Ms Analysis ◽  
Icp Ms ◽  
Different Types

The purpose of this study was to characterize some types of biomass wastes resulted from different activities such as: agriculture, forestry and food industry using thermogravimetric and ICP-MS analyses. Also, it was optimized an ICP-MS method for the determination of As, Cd and Pb from biomass ash samples. The ICP-MS analysis revealed that the highest concentration of metals (As, Cd, Pb) was recorded in the wood waste ash sample, also the thermogravimetric analysis indicated that the highest amount of ash was obtained for the same sample (26.82%). The biomass wastes mentioned in this study are alternative recyclable materials, reusable as pellets and briquettes. Keywords: ash, biomass, ICP-MS, minor elements, TG

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