scholarly journals DETERMINATION OF ANTIOXIDANT CAPACITY AND GALLIC ACID CONTENT IN ETHANOLIC EXTRACT OF PUNICA GRANATUM L. LEAF

2018 ◽  
Vol 11 (4) ◽  
pp. 319
Author(s):  
Sreedevi P ◽  
Vijayalakshmi K
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Research Work ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Hplc Method ◽  
Ic50 Values

 Objective: The present research work was carried out to evaluate the antioxidant potential of ethanolic extract of Punica granatum leaf (EPGL) that belongs to the family of Punicaceae and determine its gallic acid (GA) content using chromatography method.Methods: Six complementary test systems, namely, 1,1-diphenyl 2-picryl hydrazine (DPPH), hydrogen peroxide (H2O2), superoxide (SO), nitric oxide (NO), hydroxyl (OH) radical scavenging, and reducing power activities were analyzed for determining antioxidant activity of EPGL. The simple and novel chromatography techniques such as thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used for the detection and quantification of GA in EPGL.Results: IC50 values of EPGL were found to be 136 μg/ml for DPPH, 88.5 μg/ml for H2O2, 16.8 μg/ml for SO, 96.5 μg/ml for NO, and 143 μg/ml for OH. The ascorbic acid (AA) and GA were used as standard compounds. The absorbance of EPGL in reducing power assay was found to be 0.18 at 100 μg/ ml, while AA and GA absorbance was found to be 0.24 and 0.4 at the same concentration. The amount of GA in EPGL was found to be 1.189 mg/g.Conclusion: These findings suggested that EPGL could be a potential source of natural antioxidant, and HPLC method used for the determination of GA is simple, precise, accurate, and suitable for routine analysis of GA in EPGL.

Novel Determination of Anti-Hypertensive Combination; Benidipine Hydrochloride and Nebivolol Hydrochloride by High Performance Chromatographic Method

2021 ◽  
pp. 5433-5438
Author(s):  
V.L.N. Balaji Gupta Tiruveedhi ◽  
Venkateswara Rao Battula ◽  
Kishore Babu Bonige ◽  
Tejeswarudu B.
Keyword(s):  
High Performance ◽  
Degradation Products ◽  
Research Work ◽  
Hplc Method ◽  
Assay Method ◽  
Injection Quantity ◽  
Relative Standard ◽  
Nebivolol Hydrochloride ◽  
Concentration Series

This research work was designed to establish and validate a novel stability indicating RP-HPLC method for the combined determination of Benidipine hydrochloride (BHE) and Nebivolol hydrochloride (NHE) in bulk and tablets, dependent on ICH guidelines.The assay method to analyse BHE and NHE was optimized with isocratic elution using acetonitrile: 0.1M acetate buffer (45:55, pH 5.1), Lichrospher ODS RP-18 column and flow pace of 1 ml/min. Total time for single run was 14 min. The injection quantity was 20μl, and was detected at 249nm. The method was verified on a concentration series of 1.25-10μg/ml (NHE) and 1.0-10μg/ml (BHE) for precision, accuracy and linearity. The LOD values were 0.059µg/ml and 0.028µg/ml for NHE and BHE, respectively. The LOQ values were 0.196µg/ml for NHE and 0.094µg/ml for BHE. The recovery percentages were 98.60-100.11% (BHE) and 98.94-101.50% (NHE) with relative standard deviation 0.250-0.694% (BHE) and 0.183-0.400% (NHE). The method was also observed to be efficient, and was sufficiently specific to measure BHE and NHE in the presence of stress-produced degradation products.

scholarly journals Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.2270-35.4763 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.47 mg/g dry weight with the lowest value in M. foetida2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

scholarly journals Phytochemical screening and antioxidant profiling of Sumatran wild mangoes (Mangifera spp.): a potential source for medicine antidegenerative effects

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220 ◽  
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida 3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16 mg/g dry weight with the lowest value in M. foetida 2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of drugs that have antidegenerative effects.

scholarly journals Gallic Acid-Dextran Conjugate: Green Synthesis of a Novel Antioxidant Molecule

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 478
Author(s):  
Moacir Fernandes Queiroz ◽  
Diego Araujo Sabry ◽  
Guilherme Lanzi Sassaki ◽  
Hugo Alexandre Oliveira Rocha ◽  
Leandro Silva Costa
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Total Antioxidant ◽  
Potential Applications ◽  
A Minor

A novel derivative of dextran, dextran–gallic acid (Dex–Gal), obtained from simple conjugation with gallic acid, was synthesized by an efficient free radical-mediated method. To verify the synthesis of Dex–Gal, 1H-nuclear magnetic resonance (1H-NMR), Fourier transform infrared (FTIR) spectrometry, and high-performance size-exclusion chromatography (HPSEC) were employed. The results revealed the conjugation of gallic acid with the 15.5 kDa dextran from Leuconostoc mesenteroides. Dex–Gal had a molecular weight of 11.2 kDa, indicating that the conjugation reaction was accompanied by a minor degradation of Dex–Gal. In addition, Dex–Gal contained 36.8 ± 1.4 mg gallic acid per gram dextran. These molecules were also evaluated as antioxidants using total antioxidant capacity (TAC), reducing power, ferric chelation, and superoxide radical-scavenging assays. Both polysaccharides had no ferric chelation activity. In addition, Dex–Gal was more efficient as an antioxidant agent in TAC (13 times) and was more efficient than dextran in superoxide radical-scavenging (60 times) and reducing power (90 times) assays. These data demonstrate that Dex–Gal is a natural-compound-based antioxidant with potential applications in the pharmaceutical, cosmetic, and food industries.

Extraction and identification of phenol compounds from Bitter Melon Momordica charantia fruits and their role as antioxidants

2013 ◽  
Vol 7 (1) ◽  
pp. 41-47
Author(s):  
Kais kassim Ghaima ◽  
Mohamad Ibrahim Nader ◽  
Rami Ali Taqi ◽  
Sanaa Abdulhusain Gatta
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Aqueous Extract ◽  
High Performance ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Hplc Method ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Phenolic Constituents

The antioxidant and free radical scavenging activities of Bitter Melon Momordica charantia exracts including phenolic compounds, ethanolic and aqueous were studied. Phenolic compounds were extracted, purified and identified by High Performance Liquid Chromatography (HPLC) method. The main phenolic constituents, which were present in the fruit extract of bitter melon, were gallic acid, protocatechuic acid, gentisic acid, catechin, chlorogenic acid and epicatechin. The results clearly indicated that phenolic compounds have an effective antioxidant activity by using Ferric Thiocyanate (FTC) method. Phenolic compounds caused 91.25% lipid peroxidation inhibition of linoleic acid emulsion. This activity was greater than ethanolic extract 82.5%, - tocopherol 70% and aqueous extract 49.58%. Also the phenolic compounds revealed obvious activity for H2O2 scavenging 68.8% in comparison with - tocopherol 45.3%, ethanolic extract 52.6% and aqueous extract 36.2%. These results confirmed the important role of phenolic compounds as antioxidants and the most antioxidant activity of bitter melon fruits belong to these compounds.

scholarly journals Development and validation of Stability Indicating HPLC method for determination of Ellagic and Gallic acid in Jambul seed

2015 ◽  
Vol 3 (3) ◽  
pp. 434-438 ◽  
Author(s):  
Mrinalini Damle ◽  
Nilam Dalavi
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Limit Of Detection ◽  
Ethanolic Extract ◽  
Hplc Method ◽  
Uv Detector ◽  
Stability Indicating ◽  
Stress Degradation ◽  
Development And Validation

Ellagic and Gallic acid are main phytoconstituents of S.cumini seeds. These are the phenolic compounds. An approach for the stress degradation was successfully applied for the development of stability indicating HPLC method for the determination of Ellagic and Gallic acid. Sample was resolved on a Hypersil C18 (250*4.6 mm particle size 5?) column. The mobile phase consisted of 1% OPA and ACN and in the ratio of 70:30 v/v which was sonicated to degas and delivered at a flow rate of 1ml/min at ambient temperature. The retention time of Ellagic acid and Gallic acid was 3.1±0.05 & 4.1±0.05 minutes. Studies were performed using an HPLC system equipped with a UV detector; the response was monitored at 271nm. The method is specific to Ellagic and Gallic acid; it is able to resolve the peak from ethanolic extract of s.cumini seeds and formulation. The calibration curve was linear over the concentration range of 8-24 ?g/ml (r2=0.997, 0.998 resp). The limit of detection for  Ellagic acid and Gallic acid   was found to be 0.25?g/ml, 0.15?g/ml resp. and the quantification limit was about 0.75?g/m, 0.49?g/ml. The accuracy of the method was established based on the recovery studies. The markers were subjected to acid, base, neutral hydrolysis, oxidation, thermal degradation and photolysis. The method was successfully validated according to ICH guidelines Q2 (R1).Int J Appl Sci Biotechnol, Vol 3(3): 434-438

Simultaneous Determination of Gallic Acid and Quercetin in Leaf Extract of Madhuca longifolia from Myanmar by HPLC-DAD: Method Development and Validation

2020 ◽  
Vol 859 ◽  
pp. 51-56
Author(s):  
Aye Thida ◽  
Onoomar Toyama ◽  
Malai Satiraphan
Keyword(s):  
Gallic Acid ◽  
Leaf Extract ◽  
Method Development ◽  
Research Work ◽  
Hplc Method ◽  
Madhuca Longifolia ◽  
Method Development And Validation ◽  
Aqueous Leaf Extract ◽  
Development And Validation

Madhuca longifolia (J.Koenig ex L.) Macbr. belongs to the Sapotaceae family. This research work focused on the determination of gallic acid and quercetin in an aqueous leaf extract of M. longilolia. The development and validation of the analytical method using HPLC-DAD have been performed. The validated method was applied to determine both compounds in the leaves of M. longifolia. Optimized HPLC conditions were developed and validated for specificity, linearity, sensitivity, precision and accuracy. The linearity ranges and linear regression value were 3.125 to 100 μg/mL with r2=1 for gallic acid and 0.78 to 50 μg/mL with r2=0.9999 for quercetin, respectively. The LOD and LOQ were 0.24 and 0.73 μg/mL for gallic acid and 0.21 and 0.63 μg/mL for quercetin. Intra and inter-day precision were in the range of 0.18 to 1.76. The recovery percentage were 103.86% to 104.98% for gallic acid and 100.10% to 102.97% for quercetin. The concentration ranges of gallic acid and quercetin in the extracts were 207.95 to 405.79 mg/100 g and 7.31 to 20.56 mg/100 g, respectively. The developed HPLC method was considered to be accurate, precise and reliable for the determination of gallic acid and quercetin in M. longifolia aqueous leaf extract. This may be the first-time report for HPLC-DAD method development for the determination of bioactive phenolic compounds in Madhuca longifolia from Myanmar.

scholarly journals Determination of antioxidant and antimicrobial properties of Agaricus bisporus from Romanian markets

2012 ◽  
Vol 23 (1) ◽  
pp. 47-52
Author(s):  
Emanuel Vamanu
Keyword(s):  
Agaricus Bisporus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Freeze Dried ◽  
Nitric Oxide Radical

AbstractThe antioxidant action of ethanolic extract from Agaricus bisporus which is available in thesupermarkets of Bucharest, Romania, was assessed by determining its reducing power and its radical scavenging activity. The determinations were made by analyzing the freeze-dried ethanolic extract. The radical scavenging activity was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), superoxide radical, nitric oxide radical and hydrogen peroxide scavenging assays. Total phenols, flavonoids, ascorbic acid, β-carotene and lycopene were also determined. The ethanolic extract from A. bisporus could be a natural antioxidant and antimicrobial source against the tested organisms, as demonstrated by the minimum inhibitory concentration values.

scholarly journals Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida 3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16 mg/g dry weight with the lowest value in M. foetida 2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT ACTIVITY AND ACUTE ORAL TOXICITY OF POMEGRANATE (PUNICA GRANATUM L.) FRUIT PEEL EXTRACT

2019 ◽  
pp. 7-14
Author(s):  
Hai Trieu Ly ◽  
Tuan Anh Vo ◽  
Viet Hong Phong Nguyen ◽  
Thi My Sa Pham ◽  
Bich Thao Lam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Punica Granatum ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Fruit Peel ◽  
Total Polyphenol ◽  
Punica Granatum L

Background: The natural antioxidants have an important role in the prevention of many diseases. The aim of study is to investigate phytochemical components, antioxidant activity and acute oral toxicity of Pomegranate (Punica granatum L.) fruit peel (PFP) extract. Materials and methods: Phytochemicals of PFP were determined by qualitative chemical tests, thin layer chromatography, total polyphenol and flavonoid contents. The PFP extract was evaluated for antioxidant activity by DPPH assay and MDA assay. In vivo acute oral toxicity test was conducted using Karber-Behrens method to determine LD50. Results: Results illustrated that PFP mainly contains flavonoids, alkaloids, tannins, triterpenes, saponins, and coumarins. PFP extract exhibited the total polyphenol and flavonoid contents with 189.97 mg gallic acid equivalent/g dry weight and 9.42 mg quercetin equivalent/g dry weight, respectively. The DPPH free radical scavenging and anti-lipid peroxidation activities of PFP extract were expressed with IC50 value of 4.80 μg/mL and 0.38 μg/ mL, sequentially. Simultaneously, the Dmax (the maximum dose administered to mice that no toxicity was observed) of PFP extract was determined to be 21.28 g/kg, equivalent to 35.64 g dried herb. Conclusion: The PFP extract is relatively safe and revealed high antioxidant activity. Key words: Punica granatum L.; polyphenols; flavonoids; gallic acid; quercetin; antioxidant activity; acute oral toxicity

Sign in / Sign up

Export Citation Format

Share Document