scholarly journals SYNTHESIS AND STABILITY TEST OF RESVERATROL-CONJUGATED GOLD NANOPARTICLE WITH POLYVINYL ALCOHOL STABILIZATION

2020 ◽  
pp. 221-229
Author(s):  
SUTRIYO SUTRIYO ◽  
RADITYA ISWANDANA ◽  
MARINA DWI HAFSHARI
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Physical Stability ◽  
Drug Efficacy ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Chloroauric Acid ◽  
Stability Tests ◽  
The Stability ◽  
Effective Site

Objective: Gold nanoparticles (AuNPs) have been developed as a promising effective site-specific drug to increase drug efficacy and reduce potentialside effects. However, AuNPs are unstable because they easily aggregate. This study aims to produce stable resveratrol (RSV)-conjugated AuNPs usingpolyvinyl alcohol (PVA).Methods: AuNPs were synthesized using the Turkevich method, which involves the reduction of chloroauric acid with sodium citrate as a reductor.AuNPs were then modified with PVA as a stabilizing agent and conjugated with RSV as a drug model in the carrier system. The formed conjugates werecharacterized using ultraviolet–visible spectrophotometry, Fourier transform infrared spectroscopy, particle size analysis, and high-performanceliquid chromatography. Furthermore, stability tests were performed in various media (2% bovine serum albumin [BSA], 1% cysteine, phosphatebufferedsaline [PBS] pH 4, PBS pH 7.4, and 0.9% NaCl) for 28 days.Results: RSV–AuNPs–PVA had a particle size of 78.75 nm, with polydispersity index (PDI) of 0.356, zeta potential of −36.1 mV, and highest entrapmentefficiency of 78.1%±0.7. RSV–AuNPs without PVA stabilization had a particle size of 51.97 nm, with PDI of 0.694 and zeta potential of −24.6 mV. Theresults of the stability tests demonstrated that RSV–AuNPs–PVA was stable in 2% BSA, PBS pH 7.4, PBS pH 4, and NaCl 0.9% and were unstable in 1%cysteine. RSV–AuNPs without PVA were stable in 2% BSA and PBS pH 7.4 and unstable in 1% cysteine, PBS pH 4, and 0.9% NaCl.Conclusion: PVA can improve the physical stability of RSV-AuNPs conjugates.

scholarly journals Preparation and Characterization of Betel Leaves (Piper betle Linn) Extract Nanoparticle with Ionic Gelation Method

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Dwi Saryanti ◽  
Dian Nugraheni ◽  
Nisa Sindi Astuti
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Leaf Extract ◽  
Particle Morphology ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Piper Betle ◽  
Ionic Gelation ◽  
Betel Leaf

Nanoparticles are used in drug delivery which can increase mass transfer so increase the absorption and effectiveness of the drug. Therefore, its prospect to improve antibacterial and antioxidants activities of betel leaves. The research aimed to preparation and characterization of betel leaf extract using ionic gelation technique. The formulation of nanoparticles from betel leaf extract with ionic gelation method using alginate and CaCl2 with a ratio of 2.5: 1. The characterization of the nanoparticles includes particle size analysis, zeta potential, particle morphology and determination of flavonoid content. Particle size analysis demonstrated that the betel leaf extract nanoparticles had a particle size of 243,03 ± 1,48 nm, zeta potential of -23,0 ± 0,35 mV and morphology of particle showed that a flat shape. The betle leaf exctract nanoparticle positively contained flavonoid with Rf 0.7 equivalent to quercetin. The betel leaf extract can be made nanoparticles with ionic gelation method using alginate and CaCl2.

scholarly journals Zeta-potential and Particle Size Analysis of Human Amelogenins

2009 ◽  
Vol 89 (2) ◽  
pp. 149-153 ◽  
Author(s):  
V. Uskoković ◽  
Z. Castiglione ◽  
P. Cubas ◽  
L. Zhu ◽  
W. Li ◽  
...  
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Self Assembly ◽  
Electrostatic Interactions ◽  
Particle Size Analysis ◽  
Full Length ◽  
Spherical Particles ◽  
Size Analysis ◽  
Hierarchical Assembly ◽  
Ph Range

The developing enamel matrix is a highly dynamic system mainly composed of the full-length amelogenin and its proteolytic cleavage products. In this study, size, zeta-potential, and the isoelectric points of nanoparticles of the recombinant full-length human amelogenin (rH174) and two proteolytic products (rH163 and rH146) were analyzed by dynamic light-scattering and electrokinetic measurements. We tested the hypothesis that zeta-potential may be used as a control parameter in directing the self-assembly of amelogenins. Extensive aggregation of amelogenin molecules with the particle size reaching about one micron occurred at a mildly acidic to neutral pH, and coincided with the red shift of the internal fluorescence. Zeta-potential was between ± 15 mV in the same pH range, indicating that amelogenin aggregation occurred when surface potentials were minimal. This suggests that electrostatic interactions may be another crucial factor, aside from hydrophobic interaction, in the aggregation and hierarchical assembly of spherical particles of amelogenins into supramolecular structures of a higher order.

scholarly journals FORMULATION AND OPTIMIZATION OF NIFEDIPINE LOADED NANOCARRIERS

2021 ◽  
pp. 311-317
Author(s):  
ASHWINI JADHAV ◽  
BINOY VARGHESE CHERIYAN
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Physicochemical Characteristics ◽  
Good Choice ◽  
The Stability

Objective: The main aim of this study to formulate a nifedipine-loaded nanocarrier for improving solubility and bioavailability. Methods: To improve the solubility of drug, nifedipine-loaded nanocarrier (lipotomes) were prepared by using the film lipid hydration technique. lipotomes were prepared by using tween 80, which is used for increasing solubility and cetyl alcohol for lipophilic environment. Drug excipients interaction determined by FTIR. lipotomes were characterized for particle size, Entrapment efficiency and zeta potential. lipotomes were optimized by using Design-Expert 12 software. Optimized formula further lyophilized by using different cyroproyectant to improve the stability and oral administration of the drug. Results: FTIR shows there was no interaction between formulation ingredients. Mean particle size, entrapment efficiency, zeta potential was determined and found to be 308.1 nm, 96.7%, 20.1mV, respectively. Surface morphology of lipotomes was observed by a scanning electron microscope (SEM). Optimized lipotomes was lyophilized with Mannitol (8% w/v) was the ideal cryoprotectant to retain the physicochemical characteristics of the OLT formulation after lyophilization. Conclusion: Nifedipine loaded nanocarrier was successfully prepared, using film hydration method. Which have good particle size, EE% and zeta potential. After lyophilization no significant changes was observed in particle size with good physical stability, so it could be a good choice for conventional drug delivery system by doing further investigation as in vitro and in vivo study

Preparation of ZnO Nanosol Dispersed in Aqueous Medium and its Dispersion Characteristics

2013 ◽  
Vol 481 ◽  
pp. 66-71 ◽  
Author(s):  
Hyeon Ha Lim ◽  
Seung Ho Lee ◽  
Hyung Mi Lim ◽  
Dae Sung Kim
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Aqueous Medium ◽  
Average Particle Size ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Average Particle ◽  
Dispersion Parameters ◽  
Ft Ir ◽  
Zno Powder

Highly dispersed ZnO nanosol, having an average particle size of about 40nm based on Particle Size Analysis (PSA), was prepared under aqueous solution without the removal of large particles by centrifugation. The ZnO nanosol was investigated on the effect of various dispersion parameters, i.e. milling time, dispersant content, pH, etc. The nanosol was effectively dispersed at 20~30 wt% of dispersant amount compared to ZnO content under ball-milling for 10 hours at pH 10. The dispersion characteristic of the nanosol was investigated into particle size and zeta potential. We discussed on the dispersion behavior of (-) charged ZnO particle surrounded by dispersant together with the variation of (-) charged dispersant's amount in aqueous medium at pH 10. The ZnO powder and nanosol were characterized by SEM, TEM, TGA, FT-IR, PSA and Zeta-potential.

scholarly journals Fabrication of Biopolymer Based Nanoparticles for the Entrapment of Chromium and Iron Supplements

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 707
Author(s):  
Nishay Patel ◽  
Mohammed Gulrez Zariwala ◽  
Hisham Al-Obaidi
Keyword(s):  
Drug Delivery ◽  
Particle Size ◽  
Zeta Potential ◽  
Whey Protein ◽  
Iron Absorption ◽  
Oral Drug Delivery ◽  
Particle Size Analysis ◽  
Protein Isolate ◽  
Oral Drug ◽  
Size Analysis

The objective of this study was to encapsulate iron and chromium into novel nanoparticles formulated using chitosan (CS), dextran sulfate (DS) and whey protein isolate (WPI) for oral drug delivery. Empty and loaded CS-DS nanoparticles were prepared via complex coacervation whilst whey protein nanocarriers were produced by a modified thermal processing method using chitosan. The physiochemical properties of the particles were characterized to determine the effects of formulation variables, including biopolymer ratio on particle size and zeta potential. Permeability studies were also undertaken on the most stable whey protein–iron nanoparticles by measuring Caco-2 ferritin formation. A particle size analysis revealed that the majority of samples were sub-micron sized, ranging from 420–2400 nm for CS-DS particles and 220–1000 nm for WPI-CS samples. As expected, a higher chitosan concentration conferred a 17% more positive zeta potential on chromium-entrapped WPI nanoparticles, whilst a higher dextran volume decreased the size of CS-DS nanoparticles by 32%. The addition of iron also caused a significant increase in size for all samples, as seen where the loaded WPI samples were 296 nm larger than the empty particles. Caco-2 iron absorption revealed that one formulation, which had the lowest particle size (226 ± 10 nm), caused a 64% greater iron absorption compared to the ferrous sulfate standard. This study describes, for the first time, the novel design of chromium- and iron-entrapped nanoparticles, which could act as novel systems for oral drug delivery.

scholarly journals Physical characterization and permeability of lupeol by use of organogel-based emulsions (o/w)

2020 ◽  
Vol 9 (1) ◽  
pp. 861-865
Keyword(s):  
Cell Culture ◽  
Particle Size ◽  
Zeta Potential ◽  
Optical Microscopy ◽  
Health Effects ◽  
Canola Oil ◽  
Particle Size Analysis ◽  
Controlled Delivery ◽  
Size Analysis ◽  
Naturally Occurring

Lupeol, is naturally occurring compound present in plants, with some beneficial health effects. In the present study, organogel-based emulsions (o/w) were obtained from canola oil. Particle size analysis, rheology test, optical microscopy, and permeability of nutraceuticals by cell culture and UPLC-MS/MS were done. Particle hydrodynamic diameters obtained in organogel-based emulsions loaded with nutraceuticals were <200nm, Polydispersity Index (PdI) was around 0.25-0.4, and Zeta Potential (ZP) was about -19 to -25mV. Droplets in emulsions presented spherical shapes and adjusted to the Herschel-Bulkley model. Interestingly, permeability of lupeol was increased compared with its crystalline; therefore organogel-based emulsions loaded with lupeol have potential for controlled delivery of this nutraceutical.

Design of Nanoemulsions through Combination of Fixed-Volatile Oils

2011 ◽  
Vol 486 ◽  
pp. 123-126 ◽  
Author(s):  
Sirikarn Pengon ◽  
Chutima Limmatvapirat ◽  
Sontaya Limmatvapirat
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Soybean Oil ◽  
High Surface ◽  
Physical Stability ◽  
Visual Observation ◽  
Volatile Oils ◽  
Potential Measurement ◽  
Tea Tree ◽  
The Stability

The aim of study was to comparatively evaluate emulsions containing volatile oils e.g. peppermint oil, optamint and tea tree oil, and those containing the combination of volatile oils with soybean oil. The emulsions were prepared and then characterized by monitoring of particle size, visual observation of creaming and cracking, and zeta potential measurement. The results showed that particle size of emulsions containing only volatile oils was in the micron range (3-10 mm). However, the particle size was decreased to less than 300 nm after incorporation with soybean oil (1:1 by weight), suggesting the formation of nanoemulsions. The nanoemulsions containing both volatile oils and soybean oil demonstrated almost 100% creaming and did not show any evidence of cracking. Additionally, percent creaming and particle size were not significantly changed even after accelerated stability testing. The results indicated the good physical stability. The stabilization of emulsion might relate with the high surface charge as observed by higher zeta potential of emulsions prepared from volatile oils combined with soybeans oil. Glycerides from soybean should give more negative charge to the oleaginous phase that help preventing aggregation of oil droplets and thus increased the stability of nanoemulsions. In conclusion, the results from this study could provide the basic guideline for preparation of stable nanoemulsions for food, cosmetic and pharmaceutical fields in the near future.

Sign in / Sign up

Export Citation Format

Share Document