scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEDYOTIS CORYMBOSA, INULA RACEMOSE, HOLOSTEMMA ADAKODIEN MEDICINAL PLANTS

2019 ◽  
pp. 25-29
Author(s):  
AJIT KUMAR MARISETTI ◽  
VENKATARATHANAMMA V.
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Microbial Activity ◽  
Positive Control ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Activity Screening ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

Objective: To evaluate the antibacterial and antifungal activity of three different medicinal plants invitro. Oil extracts of the plants from three different families (Hedyotis corymbosa, Inula racemose, Holostemma adakodien) are used to find the antibacterial and antifungal activity. Methods: Study of anti-microbial activity using agar cup diffusion method, inoculums preparation, Anti-bacterial activity screening (well diffusion method), Anti-fungal activity screening (well diffusion method) are the different methods which are used to find the anti-bacterial and anti-fungal activities of the oil extracts of the above medicinal plants. The activity of these oil extracts and the selected antibiotics are evaluated against four bacterial strains (E. Coli, S. aureus and P. aeruginosa) and three Candida fungal strains (albicans, parapsilosis and tropicalis.). The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Results: The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Further studies are needed to explore the novel antibacterial bioactive molecules. Conclusion: The plants Hedyotis corymbosa, Inula racemose and Holostemma adakodien have shown significant anti-bacterial and anti-fungal activity.

scholarly journals Antibacterial and Antifungal Activity of Plant Extracts from Spinacia oleraceae L. (Amaranthaceae)

2021 ◽  
pp. 94-100
Author(s):  
Firza Shafique ◽  
Uzma Naureen ◽  
Annam Zikrea ◽  
Sohail Akhter ◽  
Tanzila Rafique ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Bacterial Species ◽  
Ethanolic Extract ◽  
Fungal Species ◽  
Diffusion Method ◽  
Root Extract ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Good Efficiency ◽  
Antibacterial And Antifungal

Spinach (Spinacia oleracea L. SO) leaves represent an important dietary source, have high nutritional value and antimicrobial properties. Spinach leaves have been used in the treatment of human diseases since ancient times. Here, the aim of this study was to evaluate the antimicrobial and antifungal activities of ethanolic extract of Spinach oleracea leaves by determining the minimum inhibitory concentrations (MICs) using well diffusion method against bacterial        species Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus and Klebseilla pneumonia and fungal species Aspergillus fumigatus, Aspergillus niger,        Candida albicans and Fusarium oxysporum. We evaluated from present data that ethanolic extract of root showed zone of inhibition ranges from 6 mm to 21 mm, ethanolic extract of stem showed zone of inhibition ranges from 8 mm to 21 mm and ethanolic extract of leaf showed zone of inhibition ranges from 9 mm to 22 mm from concentration 25 mg/ml to 100mg/ml. Leaf extract has high antibacterial and antifungal activity against bacterial and fungal species while root extract has low antibacterial and antifungal activity against bacterial and fungal species. Activity of plant extract was increased by the increasing concentration of extracts. Very low zone of inhibition was found at concentration 25 mg/1 ml DMSO which ranges from 6 mm to 14 mm while very high zone of inhibition was found at concentration 100 mg/ml which ranges from 6 mm to 22 mm. So, ethanolic extracts of Spinach oleraceae has good efficiency against bacterial and fungal species.

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF SILVER NANOPARTICLES AGAINST NEISSERIA GONORRHOEAE AND CANDIDA ALBICANS

2020 ◽  
Vol 8 (6) ◽  
pp. 179-187
Author(s):  
Titik Taufikurohmah ◽  
Tasha Anandya Tantyani
Keyword(s):  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Neisseria Gonorrhoeae ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Disk Diffusion Method ◽  
Fungal Inhibition ◽  
Inhibition Response ◽  
Antibacterial And Antifungal

This Research on the antibacterial and antifungal activity of nanosilver against Neisseria gonorrhoeae and Candida albincas fungi has been carried out. The purpose of this study was to determine antibacterial activity of nanosilver against Neisseria gonorrhoeae and antifungal activity against Candida albincas. Synthesis Nanosilver uses bottom up method and characterized using UV-Vis Spectrophotometer. Nanosliver concentrations used were 30, 40, 50, and 60 ppm. Antibacterial and antifungal activity tests using disk diffusion method. Observations obtained in form of the presence or absence of clear zones formed around paper discs indicate the inhibition of nanosilver on microbial growth. The results of testing the antifungal activity of Candida albicans on nanosilver with concentrations of 30, 40, 50 and 60 ppm resulted in clear zones of 9.73 nm, 11.46 nm, 11.93 nm, and 13 nm with fungal inhibition response categories is medium and strong. The results antibacterial activity test of Neisseria gonorrhoeae on nanosilver with concentrations of 30, 40, 50 and 60 ppm did not show any clear zone around the disc, it showed that nanosilver in this study did not have antibacterial activity against Neisseria gonorrhoeae.

scholarly journals Synthesis, Spectral Characterization, and Antibacterial and Antifungal Studies of PANI/V2O5Nanocomposites

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Chakradhar Sridhar ◽  
Nagesh Gunvanthrao Yernale ◽  
M. V. N. Ambika Prasad
Keyword(s):  
Antifungal Activity ◽  
Sol Gel ◽  
Chemical Oxidation ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
X Ray Diffraction ◽  
Xrd Techniques ◽  
Antibacterial And Antifungal

The present study deals with the synthesis and characterization of nanocrystalline vanadium pentoxide (V2O5) nanoparticles and their antibacterial and antifungal activity onStaphylococcus aureus and Aspergillus niger, respectively, by agar diffusion method. The metal oxide has been synthesized by employing the sol-gel method, polyaniline (PANI) has been synthesized by chemical oxidation, and PANI/V2O5composites have been synthesized byin situpolymerization method with different ratios (10, 20, 30, 40, and 50 weight%) of V2O5in PANI. The newly prepared composites were characterized by FTIR and powder X-ray diffraction (P-XRD) techniques and are found to be formed of PANI/V2O5nanocomposites, and also the compounds showed moderate antibacterial and antifungal activity.

scholarly journals Screening of Antimicrobial Activity of Various Extracts of the Stem Justicia gendarussa

2019 ◽  
pp. 1-7
Author(s):  
Dhanapal Venkatachalam ◽  
Akhib Rahman ◽  
Basil Sunny ◽  
Jensy Jacob ◽  
Nikhil Kuriyan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Antimicrobial Agents ◽  
Pathogenic Fungus ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Standard Drug ◽  
Antibacterial And Antifungal

Objective: The present study was designed to check in-vitro efficacy of Antibacterial and antifungal activity of aqueous and hexane extracts of stem of Justicia gendarussa against selected bacterial and fungal strains. Justicia gendarussa, belonging to the family Acanthaceae, commonly known as ‘‘vatham kolli’ was used in treatment of bronchitis, inflammation, eye diseases, ear ache, vaginal discharges, rheumatism, dysentery, eczema and jaundice. Methodology: Hexane and aqueous extracts of Justicia gendarussa was used for antimicrobial screening. Antibacterial activity was tested against pathogenic bacterial strains Eschericha coli and Staphylococcus aureus. Also antifungal activity was tested against one human pathogenic fungal strain i.e. Candida albicans. Antibacterial and antifungal activities of Justicia gendarussa extract was carried out by using disc diffusion method. Results: Aqueous extract of Justicia gendarussa showed the largest zone of inhibition (5mm) against Eschericha coli, at 50µgm/ml and 3mmzone of inhibition against S. aures, at 50µgm/ml. Hexane extract does not   showing activity against to human pathogenic E. coli and S. aures, at 50µgm/ml. Aqueous extract has shown more potent antibacterial activity against Eschericha coli. Aqueous extract was found to be more effective against human pathogenic fungus Candida albicuns than hexane extract and standard drug. The zone of inhibition of Aqueous extract was 11mm and the zone of inhibition of hexane extract was7mm and standard only 2mm.The phytochemical screening demonstrated the presence of different types of compounds like terpenoids, tannins, and flavonoids which may contribute for the anti-microbial action of this plant. Conclusions: These findings provide scientific evidence of traditional use of Justicia gendarussa and also indicate the potential of this plant for the development of antimicrobial agents.

scholarly journals ANTIMICROBIAL ACTIVITY OF SYZYGIUM GARDNERI Thw. (MYRTACEAE).

2015 ◽  
Vol 2 (1) ◽  
pp. 53-55
Author(s):  
Asha devi V ◽  
Shalimol A ◽  
Arumugasamy K ◽  
Nantha kumar R ◽  
Abdul kaffoor H
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Positive Control ◽  
Antibacterial And Antifungal Activity ◽  
Penicillium Notatum ◽  
Antibacterial And Antifungal ◽  
Alternaria Alternate

The present study was evaluated the antibacterial and antifungal activity of various extracts of S.gardneri against four different bactria and fungal strains like Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella para typhi b, Alternaria alternate, Aspergillus flavus, Penicillium notatum and Cladosporium carrionii. All the results were compared with respective positive control.

Evaluation of Antibacterial and Antifungal Activity of Moringa Concanensis

2021 ◽  
Vol 14 (4) ◽  
pp. 5566-5570
Author(s):  
Bindu Singh Yadav ◽  
Yeddu Trilochana ◽  
Kiran Kumari ◽  
Saurabh Singh ◽  
Anupam K Sachan
Keyword(s):  
Antifungal Activity ◽  
Bacterial Infections ◽  
Methyl Cellulose ◽  
Enterobacter Aerogenes ◽  
Chloroform Extract ◽  
High Rate ◽  
Diffusion Method ◽  
Significant Activity ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

In today’s scenario, fungal and bacterial infections are one of the most challenging pestilential diseases for the health-care professional which is increasing with a very high rate in the growing population, especially among the immune compromised people.The present work involved the study to extract active principles of Moringa concanensis and perform antibacterial and antifungal activity for various extracts. The leaves were dried powdered unexpected using solvents petroleum ether, chloroform, ethanol and water. Chemical tests were performed to ascertain presence of various classes of phyto-constituents like alkaloids, glycosides, saponins, carbohydrates, proteins, amino acids tannins, flavonoids and phenolic substances. All four extracts showed the presence of various phytochemicals responsible for multiple pharmacological activities. The extracts were reconstituted using 1% aqueous solution of carboxy methyl cellulose and were tested for antibacterial and antifungal activity against six microorganisms, namely Bacillus subtilis, Enterobacter aerogenes, Bacillus cereus, Aspergillus Flavus, Aspergillus Niger and Helminthosporium, using well diffusion method. The results of the activity are promising with chloroform extract showing highest inhibitory activity against most of the organisms. All the extracts were able to demonstrate significant activity compared to control inhibition of microorganisms tested and hold key to discover lead molecules after further scientific investigation.

scholarly journals Antibacterial and Antifungal Activity of Medicinal Orchids Growing in Nepal

2013 ◽  
Vol 29 ◽  
pp. 104-109 ◽  
Author(s):  
Ramesh Marasini ◽  
Susan Joshi
Keyword(s):  
Staphylococcus Aureus ◽  
Antifungal Activity ◽  
Vibrio Cholerae ◽  
Chemical Society ◽  
Antibacterial Activities ◽  
Good Activity ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Antibacterial And Antifungal ◽  
And Staphylococcus Aureus

Antibacterial activities of different extracts of epiphytic orchids were tested against 5 species of bacteria and antifungal activities were tested against 3 species of fungi. All orchid extracts showed good bacterial Zone of Inhibition (ZOI) against Staphylococcus aureus. The crude extracts of Pholidota imbricata and Coelogyne cristata were shown highest activity against Vibrio cholerae and Staphylococcus aureus respectively. The MIC and MBC value of the extracts of Pholidota imbricata and Coelogyne cristata were found to be 62.5 mg/ml, 31.25 mg/ml and 125 mg/ml, 250 mg/ml against Vibrio cholerae and Staphylococcus aureus respectively. Only Pholidota imbricata and Pholidota articulata extracts were shown fairly good activity but all others extracts were shown very less activity or even failed to show any activity against fungal organisms.DOI: http://dx.doi.org/10.3126/jncs.v29i0.9259Journal of Nepal Chemical Society Vol. 29, 2012 Page: 104-109 Uploaded date: 12/5/2013 

scholarly journals Antibacterial and Antifungal Activity Analysis of Essential Oil of Pogostemon cablin (Blanco) Benth

2016 ◽  
Vol 30 (1-2) ◽  
pp. 7-10 ◽  
Author(s):  
Puspa Das ◽  
Shoma Dutta ◽  
Jaripa Begum ◽  
Md Nural Anwar
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Pogostemon Cablin ◽  
Antibacterial And Antifungal

The essential oil of Pogostemon cablin (Blanco) Benth, also known as Patchouli oil was subjected for its antimicrobial investigation against a panel of ten human pathogenic bacteria and six human pathogenic fungi by Agar well diffusion method and Macrobroth dilution technique using Ampicillin (20ìg/well) and Nystatin (20ìg/well) as control. Antibacterial activity revealed that, the essential oil was more active against Gram positive bacteria than Gram negative bacteria. The largest zone of inhibition was 35 mm (against Bacillus cereus) with 20 ìl of oil. Ditermination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) showed that, Bacillus cereus exhibited the lowest MIC (250 ìg/ml) and MBC (750 ìg/ml). The oil showed moderate antifungal activity against all tested organisms. Candida albicans showed greater zone of inhibition (16 mm) than Saccharomyces cerevisiae (14 mm) with 20 ìl and Candida albicans showed lowest MIC and MFC (both were 750 ìg/ml). The zone of inhibition was 25 mm for each filamentous fungal strain with 20 ìl, except for Rhizopus oligosporus (15 mm) and the lowest MIC (250 ìg/ml) and MFC (500 ìg/ml) were reported for Aspergillus fumigatus.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 7-10

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