scholarly journals Phytochemical Analysis, Biochemical and Mineral Composition and GC-MS Profiling of Methanolic Extract of Chinese Arrowhead Sagittaria trifolia L. from Northeast China

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3025 ◽  
Author(s):  
Maqsood Ahmed ◽  
Mingshan Ji ◽  
Aatika Sikandar ◽  
Aafia Iram ◽  
Peiwen Qin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Chemical Composition ◽  
Northeast China ◽  
Biochemical Analysis ◽  
Chemical Compounds ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Human Beings

Sagittaria trifolia is a medicinal foodstuff of China and East Asia belonging to the family Alismataceae. Samples of S. trifolia tubers were collected from Meihekow, Siping, Jilin, Harbin and Wuchang from Northeast China. The current study was aimed to evaluate the qualitative and quantitative analysis, antioxidant activity, biochemical analysis and chemical composition of different populations of S. trifolia. By using Folin–Ciocalteu, aluminium chloride colourimetric and 1,1-diphenyl-1-picrylhydrazyl (DPPH), total phenol and flavonoids content and antioxidant activity was analysed. Furthermore, chemical composition, biochemical analysis and mineral substances were also determined. The results showed the presence of flavonoids, phenols, saponins, tannins, glycosides and steroids except for alkaloids and terpenoids by qualitative analysis. Quantitative analysis revealed that highest total phenol, flavonoids content and antioxidant potential identified from Meihekow, i.e., 2.307 mg GAE/g, 12.263 mg QE/g and 77.373%, respectively. Gas chromatography-mass spectrometry results showed the presence of 40 chemical compounds corresponding to 99.44% of total extract that might be responsible for antioxidant properties. Mineral and biochemical analysis revealed the presence of calcium, magnesium, potassium, sodium, iron, copper, zinc and, carbohydrate, protein, fibre and fat contents, respectively. Interestingly, all S. trifolia populations collected from different locations possess similar composition. The dietary values, phytoconstituents, antioxidant activities and nutritional and curative chemical compounds of S. trifolia are beneficial for the nutritherapy of human beings.

scholarly journals PHYSICOCHEMICAL, PHYTOCHEMICALS AND ANTIOXIDANT EVALUATION OF GUAZUMA ULMIFOLIA FRUIT

2018 ◽  
Vol 10 (9) ◽  
pp. 87
Author(s):  
Bhavani Duraiswamy ◽  
Malairajan Singanan ◽  
Vanitha Varadarajan
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Colorimetric Method ◽  
Water Soluble ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Guazuma Ulmifolia

Objective: The present study was designed to evaluate the physicochemical, qualitative, quantitative analysis and antioxidant activity of ethanol fruit extract of Guazuma ulmifolia. Studies were carried out to detect the bioactive component.Methods: The physiochemical properties such as loss on drying, extractive value for ethyl acetate, acetone, ethanol, total ash, acid insoluble ash, water-soluble ash and PH values; the phytochemical such as tannins, phenol, terpenoids, flavonoids, saponin, quinones, cardiac glycosides, coumarin, steroids and acids were found using standard methods. The total phenol and flavonoid content were estimated by Folinciocalteu and Aluminium chloride by colorimetric method. In vitro antioxidants, properties were evaluated by DPPH and FRAP assay.Results: Phytochemical analysis reveals the availability of secondary metabolites like tannins, phenol, terpenoids, flavonoids, coumarin, steroids, and cardiac glycosides in the different extract. The quantitative analysis for ethanolic fruit extract was done for total phenolic content (TPC), total flavonoids content (TFC). An ethanolic extract found to have high TPC [27.2797±0.1756] than TFC. Antioxidant activity was evaluated by DPPH assay (2, 2-diphenyl-1-picrylhydrazyl-hydrate) and FRAP (ferric Reducing Ability of Plasma).Conclusion: On the basis of this study, it was found that the Guazuma ulmifolia fruits have potential to act as a source of useful drugs due to the presence of various active phytochemical constituent, which will be helpful in preventing various diseases caused by the oxidative stress.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIACNE ACTIVITY OF LEAVES EXTRACT OF LAGERSTROEMIA INDICA

2021 ◽  
Vol 9 (2) ◽  
Author(s):  
Vandana Vishwakarma ◽  
Narendra Patel ◽  
Prabhakar Budholiya
Keyword(s):  
Quantitative Analysis ◽  
Medicinal Plants ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Test Protocol ◽  
Minor Ailments ◽  
Pharmaceutical Industries ◽  
Chloride Method

Medicinal plants have bioactive compounds which are used for curing of various human diseases and also play an important role in healing. Secondary constituents contain alkaloids, flavonoids, phenol, sapon in, steroids and tannins. Medicinal plants have anticancer, antimicrobial, antidiabetic, antidiuretic and anti-inflammation activities. The increasing interest in powerful biological activity of secondary metabolites outlined the necessity of determining their contents in medicinal plants. In Indian Ayurvedic system, Lagerstroemia Indica (Lythraceae) are well-known plants used for major and minor ailments. The aim of the present study is to examine leaf of Lagerstroemia Indica for phytochemical profile, antioxidant potential, antimicrobial including anti acne activity. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by FolinsCiocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The present study concluded that the crude extract of Lagerstroemia Indica is a rich source of secondary phytoconstituents which impart significant antioxidant potential.  The extract of Lagerstroemia Indica possess antimicrobial effectiveness againt P. acne and other microbes, In Vitro anti acne activity. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries. Keywords: Lagerstroemia Indica, Phytochemical, Antioxidant, Antiacne.

scholarly journals Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Aerial Parts of Clematis heynei and Solanum virginianum

2019 ◽  
Vol 9 (1-s) ◽  
pp. 260-264
Author(s):  
Pravin Morankar ◽  
Alok Pal Jain
Keyword(s):  
Quantitative Analysis ◽  
Secondary Metabolites ◽  
Total Phenolics ◽  
Developed Countries ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Test Protocol ◽  
Aerial Parts ◽  
Pharmaceutical Industries ◽  
Phenolics Content

The increasing interest in powerful biological activity of secondary metabolites outlined the necessity of determining their contents in medicinal plants. In the last few years, there has been an exponential growth in the field of herbal medicine and gaining popularity both in developing and developed countries because of their natural origin and less side effects. Clematis heynei (C. heynei, Ranunculaceae) is commonly known as Deccan clematis, Murhar, Morvel, Ranjaee and it is a somewhat woody climber very sparsely distributed in deciduous forests of Western Ghats, India. In the Indian system of medicine ‘Ayurveda’ this plant is used to eliminate malarial fever and headache. Different plant parts were used for treating various diseases. Solanum virginianum L. (S. virginianum, Solanaceae, Solanum xanthocarpum Schrad. & H. Wendl.) is a diffuse and very prickly under shrub. It is found growing commonly in various regions of the world on sandy soils and is distributed throughout India. The plant is used traditionally to treat asthma, chest pain, leucoderma, scorpion bite, and sterility in women. The aim of the present study is to examine C. heynei and S. virginianum aerial parts of plant for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of methanolic and aqueous extract of C. heynei was (0.592, 0.292 mg/100mg), followed by flavonoids (1.371, 0.723mg/100mg) respectively. The total phenolics content of methanolic extract of S. virginianum was (0.345mg/100mg), followed by flavonoids (0.978mg/100mg). The present study concluded that the crude extract of C. heynei and S. virginianum is a rich source of secondary phytoconstituents which impart significant antioxidant potential. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.  

scholarly journals Comparative Study on Chemical Composition and Antioxidant Activity of Annona Muricata Plant Parts Cultivated in Covenant University, Ota, Ogun State, Nigeria

2018 ◽  
Vol 6 (3) ◽  
pp. 807-815
Author(s):  
OMOTOSHO OMOLOLA ELIZABETH ◽  
IHEAGWAM FRANKLYN NONSO ◽  
NOIKI IFEOLUWA ADEBOLA ◽  
OMINI JOY JOHN
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Root Extract ◽  
Plant Parts ◽  
Antioxidant Agents

Annonamuricata plant parts possess a broad range of medicinal and biological properties. This research compared the chemical composition and antioxidant properties of Annonamuric at aparts. Proximate, mineral, total phenol and total flavonoid content as well as invitro antioxidant activity were examined. Results revealed the leaves contained significantly(p<0.05) higher composition of moisture (8.69±0.22%), ash (4.60±0.02%), protein (14.53±0.11%), crude fat (10.28±0.03%),chromium (0.38±0.05 mg/100g), nickel (1.75±0.04mg/100g), total phenol (1.01±0.03mgpyrocatechol/mL) and total flavonoid (1.12±0.03mgGAE/mL)compared to the respective values for root. Carbohydrate (9.29±0.24%), lead (0.13±0.02mg/100g) and cobalt (1.93±0.02mg/100g) composition was significantly lower(p<0.05) in the leaves compared to the respective compositions in the root. The leaf and root extract exhibited a concentration-dependent increase in hydroxyl radical scavenging activity with no observable(p<0.05)difference in their EC50 value. This study suggests the leaves of A. muricata found in Covenant University had better chemical composition when compared to the root. Nonetheless, these plant parts may be further exploited for not only their nutritive composition and mineral content but also a natural source of antioxidant agents.

scholarly journals Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
RIDWANTI BATUBARA ◽  
TENGKU ISMANELLY HANUM ◽  
ODING AFFANDI ◽  
HENNY SRI WAHYUNI
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Chemical Compounds ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Mature Leaves ◽  
Dpph Method ◽  
North Sumatra ◽  
Chemical Contents

Abstract. Batubara R, Hanum TI, Affandi O, Wahyuni HS. 2020. Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties. Biodiversitas 21: 4616-4622. The genus Wikstroemia is one of the genus of agarwood producing trees which has not been widely researched and published. One type of this genus is Wikstroemia tenuiramis Miq, which grows naturally in North Sumatra, the leaves are like other types of agarwood leaves can be used as raw material for agarwood leaves tea. A research has been conducted to determine the chemical contents contained in the young and mature leaves of agarwood plants species W. Tenuiramis. This research methods applied qualitative analysis using phytochemical screening, quantitative analysis to determine tannin content using titration method, antioxidant activity analysis using 2,2-diphenil-1-picrylhydrazil (DPPH) method, and compound tracing using Py-gas chromatography-mass spectrometry (Py-GC-MS) instruments. The qualitative phytochemical analysis results showed no difference in chemical compounds contained in both the young and mature leaves. Both the young and mature leaves contained flavonoids, triterpenoids, and tannins, and did not contain alkaloids, steroids and saponins. The young contained tannins of 1.079 ± 0.001% and the mature contained tannins of 4.645 ± 0.021%, not significant statistically. Py-GC-MS analysis showed the presence of various 30 bioactive compounds contained in both young and mature leaves. The analysis results also showed that both the young and mature leaves had very strong antioxidant activity. Our findings suggest that the two types of leaves have equal utility value of in term of chemical contents and antioxidant potentials.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING, TOTAL PHENOL AND FLAVONOID CONTENT OF MIMOSA RUBICAULIS AND REINWARDITA INDICA

2019 ◽  
pp. 54-58
Author(s):  
ROSHANI GURUNG
Keyword(s):  
Plant Species ◽  
Biological Effects ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Specific Chemical ◽  
Dry Extract

Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica. Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method. Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight followed by stem and root of M. rubicaulis i.e. 381.06±5.23 mg QE/g dry extract weight and 337.43±1.39 mg QE/g dry extract weight. Conclusion: Phytochemical analysis concluded the presence of biologically important phytoconstituents like flavonoid and phenol in both plant species. Further studies, should be carried out to isolate specific chemical constituents and should be used in different studies to explore their biological effects.

Isolation and HPLC Quantitative Analysis of Antioxidant Flavonoids from Alternanthera tenella Colla

2006 ◽  
Vol 61 (1-2) ◽  
pp. 19-25 ◽  
Author(s):  
Marcos J. Salvador ◽  
Eliane O. Ferreira ◽  
Susanne U. Mertens-Talcott ◽  
Whocely Victor De Castro ◽  
Veronika Butterweck ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Phytochemical Analysis ◽  
Spectroscopic Methods ◽  
Phenolic Contents

Abstract Phytochemical analysis of the antioxidant ethanolic extract of Alternanthera tenella Colla led to the isolation of six flavonoids, acacetin 8-C-[α-ʟ-rhamnopyranosyl-(1→2)-β-ᴅ-glucopyranoside] (1), 2″-O-α-ʟ-rhamnopyranosyl-vitexin (2), 2″-O-β-d-glucopyranosyl-vitexin (3), vitexin (4), quercetin (5) and kaempferol (6). All the structures were established by ESIMS and NMR spectroscopic methods. Antioxidant capacity of extract, fractions and isolated compounds was determined using the oxygen radical absorbance capacity (ORAC) assay and extract, fractions and flavonoids isolated showed antioxidant activity in vitro. Moreover, the total soluble phenolic contents of the extract and fractions were measured using the Folin- Ciocalteau reagent and the quantitative analysis of flavone C-glycosides major constituents was performed by HPLC

scholarly journals Qualitative and quantitative determination of secondary metabolites and antioxidant potential of Nymphaea nouchali flowers

2018 ◽  
Vol 8 (6-s) ◽  
pp. 111-115
Author(s):  
Manjeet Singh ◽  
Alok Pal Jain
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Quantitative Analysis ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Test Protocol

In the Indian ayurvedic system of medicine, Nymphaea nouchali is used for the treatment of diabetes, inflammation, liver disorders, cutaneous diseases, blenorrhagia, urinary disorders, menorrhagia, menstruation problem, as an aphrodisiac, bitter tonic, antimicrobial agent and anti antihepatotoxic effect.  The aim of the present study is to examine Nymphaea nouchali flowers for phytochemical profile, in vitro antioxidant activities. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the flowers was assessed against nitric oxide, hydrogen peroxide assay using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of flowers ethanolic extract was (18.4 mg/100mg), followed by flavonoids (12.4mg/100mg). The activities of ethanolic flowers extract against nitric oxide and hydrogen peroxide were concentration dependent with IC50 values of 68.39 and 64.54 μg/ml respectively. The present study concluded that the crude extract of Nymphaea nouchali is a potential source of natural antioxidants and this justifies its use in folkloric medicine. Keywords: Nymphaea nouchali, Phytochemical, Antioxidant, Nitric oxide, Hydrogen peroxide, Phenols, Flavonoids

scholarly journals Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Rumex Nepalensis Roots

2018 ◽  
Vol 8 (6-s) ◽  
pp. 97-100 ◽  
Author(s):  
Azher Nawaz Khan ◽  
Irm Bhat
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Total Phenolics ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Ethanolic Extract ◽  
Food Supplement ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Test Protocol

In the Indian ayurvedic system of medicine, Rumex nepalensis Spreng. (Polygonaceae) commonly known as Nepal Dock has wide-spectrum therapeutic potencies and is extensively used for centuries in traditional medicine systems. They act as a possible food supplement and are largely used in pharmaceutical industry. Extracts and metabolites from this plant exhibits pharmacological activities including anti-inflammatory, antioxidant, antibacterial, antifungal, antiviral, insecticidal, purgative, analesic, antipyretic, anti-algal, central nervous system depressant, genotoxic, wound healing and skeletal muscle relaxant activity. Due to its remarkable biological activities, it has the potential to act as a rich source of drug against life threatening diseases. The aim of the present study is to examine Rumex nepalensis roots for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of roots ethanolic extract was (1.658 mg/100mg), followed by flavonoids (1.048mg/100mg). The present study concluded that the crude extract of Rumex nepalensis is a potential source of various activates and this justifies its use in folkloric medicine. Keywords: Rumex nepalensis, Qualitative analysis, Quantitative analysis, TPC, TFC, Folins Ciocalteau

scholarly journals ANTIOXIDANT, ANTIMICROBIAL ACTIVITY AND TOTAL PHENOL AND FLAVONOIDS ANALYSIS OF SAMBUCUS NIGRA (ELDERBERRY)

2020 ◽  
pp. 35-37
Author(s):  
N. SRINIVAS GOUD ◽  
GOBIND PRASAD
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Capacity ◽  
Pseudomonas Putida ◽  
Colorimetric Method ◽  
Total Phenol ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Sambucus Nigra ◽  
Fruit Extract

Objective: The goal of this study was paying attention on the chemical characterization of phytochemical compounds and their antioxidant activity of Sambucus nigra L. Methods: Phytochemical analysis was performed by Sambucus nigra L fruit extract. Total Phenol and Flavonoids content of elderberry fruit extract also determined using Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method. Antibacterial activity was performed by disk diffusion method and Antioxidant capacity was investigated by DPPH assay, butylhydroxytoluene used as a standard. Results: The richest anthocyanin in elderberry fruits was cyanidin-3-O-sambubioside. The antioxidant capacity obtained for elderberry extract proved that elderberry shown highest antioxidant activity, being the richest anthocyanins. The antioxidant capacity of elderberry fruit methanolic extract was recorded 62.56±1.12 percentages of scavenging activity. We also investigated antibacterial activity against four species Escherichia coli, Pseudomonas putida, Bacillus cereus, and Staphylococcus aureus. There E. coli was recorded 12.0 mm and Pseudomonas putida was recorded 0.34 mm zone of inhibition. Conclusion: The conclusion of our study is that Sambucus nisgra fruit extract has very high antioxidant activity which makes it recommendable for food industry and dietary supplement.

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