scholarly journals HAR1: an insight into lncRNA genetic evolution

Epigenomics ◽  
2021 ◽  
Author(s):  
Ella Waters ◽  
Perla Pucci ◽  
Mark Hirst ◽  
Simon Chapman ◽  
Yuzhuo Wang ◽  
...  
Keyword(s):  
Neurological Disorders ◽  
Expression Patterns ◽  
Noncoding Rnas ◽  
Current State ◽  
Evolutionary Changes ◽  
Wide Range ◽  
Range Of Functions ◽  
Health And Disease ◽  
Insight Into

Long noncoding RNAs (lncRNAs) have a wide range of functions in health and disease, but many remain uncharacterized because of their complex expression patterns and structures. The genetic loci encoding lncRNAs can be subject to accelerated evolutionary changes within the human lineage. HAR1 is a region that has a significantly altered sequence compared to other primates and is a component of two overlapping lncRNA loci, HAR1A and HAR1B. Although the functions of these lncRNAs are unknown, they have been associated with neurological disorders and cancer. Here, we explore the current state of understanding of evolution in human lncRNA genes, using the HAR1 locus as the case study.

Evaporite Palynology: a case study on the Permian (Lopgingian) Zechstein Sea

2021 ◽  
pp. jgs2020-174
Author(s):  
Martha E. Gibson ◽  
David J. Bodman
Keyword(s):  
Thermal Conductivity ◽  
Heat Flux ◽  
Rapid Growth ◽  
Western Europe ◽  
Thermal Maturity ◽  
Near Surface ◽  
Wide Range ◽  
Terrestrial Environments ◽  
Insight Into

Evaporites characterize the Lopingian of Europe but present obstacles for biostratigraphic analysis. Here we present a case study for processing the Lopingian Zechstein Group evaporites of central-western Europe for the recovery of palynomorph assemblages. We demonstrate that full recovery is easily achieved with two main modes of palynomorph preservation observed; palynomorphs are either exceptionally well-preserved and orange-brown in colour, or poorly-preserved, brown-black, opaque and fragmented. The latter are reminiscent of palynomorphs of high thermal maturity. However, we propose that the intact nature of preservation is a result of the rapid growth of near-surface halite crystals, with their darkening a consequence of locally-enhanced heat flux due to the relatively high thermal conductivity of salt. This case study has enabled novel insight into an otherwise undescribed environment, and demonstrates the utility and possibility of extracting palynomorphs from a variety of rock salt types. This method should be applicable to a wide range of ancient evaporite and could also be applied to other Permian evaporite systems, which are used as analogues for extra-terrestrial environments.

scholarly journals Speech synthesis systems: disadvantages and limitations

2018 ◽  
Vol 7 (2.28) ◽  
pp. 234 ◽  
Author(s):  
Karolina Kuligowska ◽  
Paweł Kisielewicz ◽  
Aleksandra Włodarz
Keyword(s):  
Text Analysis ◽  
Speech Synthesis ◽  
Analysis Problem ◽  
Current State ◽  
Wide Range ◽  
Current Classification ◽  
Weak Points ◽  
The Impact ◽  
Further Development ◽  
Insight Into

The present speech synthesis systems can be successfully used for a wide range of diverse purposes. However, there are serious and important limitations in using various synthesizers. Many of these problems can be identified and resolved. The aim of this paper is to present the current state of development of speech synthesis systems and to examine their drawbacks and limitations. The paper dis-cusses the current classification, construction and functioning of speech synthesis systems, which gives an insight into synthesizers implemented so far. The analysis of disadvantages and limitations of speech synthesis systems focuses on identification of weak points of these systems, namely: the impact of emotions and prosody, spontaneous speech in terms of naturalness and intelligibility, preprocessing and text analysis, problem of ambiguity, natural sounding, adaptation to the situation, variety of systems, sparsely spoken languages, speech synthesis for older people, and some other minor limitations. Solving these problems stimulates further development of speech synthesis domain. 

scholarly journals New trends in regulation of agriculture

2021 ◽  
Vol 273 ◽  
pp. 08018
Author(s):  
Sergey Zyryanov ◽  
Anastasia Kalmykova ◽  
Tatyana Levonenkova ◽  
Ekaterina Kozlova ◽  
Olyesya Starodubova
Keyword(s):  
Public Institutions ◽  
Theory And Practice ◽  
State Agencies ◽  
Regulatory State ◽  
Current State ◽  
Wide Range ◽  
Range Of Functions ◽  
Retail Chains ◽  
And Control ◽  
Regulatory Trends

Regulatory state agencies propose new solutions on regulating relationship in agriculture. Countries with developed agriculture have various national public institutions with a wide range of functions. They develop public policies, set standards, issue licenses and other permissions, do inspections, protect rights of agricultural products consumers, provide support to producers, and etc. Bodies regulating relationship in agriculture act in accordance with the general regulatory trends. Their activity is influenced by modernization and reformation processes, such as deregulation in various forms, transition from a command and control model of regulation to new models providing incentives for producers to voluntarily compliance, risk-based approach introduction, etc. Regulatory tools are often not effective enough for a number of reasons. This work consists of three parts, it evaluates the current state of agriculture in specific actual areas. The first section investigates general trends in the development of regulatory theory and practice. The second section deals with the problems of protecting farmers from unfair trade practices of suppliers and retail chains. The third section presents the results of the analysis of competing provisions on environmental protection and food safety. In conclusion, the authors give a system of new ways of regulation which can replace traditional ways of deterrence.

scholarly journals Rapid Fabrication by Digital Light Processing 3D Printing of a SlipChip with Movable Ports for Local Delivery to Ex Vivo Organ Cultures

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 993
Author(s):  
Megan A Catterton ◽  
Alexander G Ball ◽  
Rebecca R Pompano
Keyword(s):  
3D Printing ◽  
Ex Vivo ◽  
Local Delivery ◽  
Tissue Slices ◽  
Digital Light Processing ◽  
Rapid Fabrication ◽  
Wide Range ◽  
Scale Levels ◽  
Range Of Functions

SlipChips are two-part microfluidic devices that can be reconfigured to change fluidic pathways for a wide range of functions, including tissue stimulation. Currently, fabrication of these devices at the prototype stage requires a skilled microfluidic technician, e.g., for wet etching or alignment steps. In most cases, SlipChip functionality requires an optically clear, smooth, and flat surface that is fluorophilic and hydrophobic. Here, we tested digital light processing (DLP) 3D printing, which is rapid, reproducible, and easily shared, as a solution for fabrication of SlipChips at the prototype stage. As a case study, we sought to fabricate a SlipChip intended for local delivery to live tissue slices through a movable microfluidic port. The device was comprised of two multi-layer components: an enclosed channel with a delivery port and a culture chamber for tissue slices with a permeable support. Once the design was optimized, we demonstrated its function by locally delivering a chemical probe to slices of hydrogel and to living tissue with up to 120 µm spatial resolution. By establishing the design principles for 3D printing of SlipChip devices, this work will enhance the ability to rapidly prototype such devices at mid-scale levels of production.

scholarly journals Fetal-Derived Immune Cells at the Roots of Lifelong Pathophysiology

2021 ◽  
Vol 9 ◽  
Author(s):  
Elvira Mass ◽  
Rebecca Gentek
Keyword(s):  
Immune Cells ◽  
Innate Immune ◽  
Hematopoietic Stem ◽  
Environmental Perturbations ◽  
Wide Range ◽  
Range Of Functions ◽  
Health And Disease ◽  
Fetal Hematopoiesis ◽  
Early Life Events

Tissue-resident innate immune cells exert a wide range of functions in both adult homeostasis and pathology. Our understanding of when and how these cellular networks are established has dramatically changed with the recognition that many lineages originate at least in part from fetal sources and self-maintain independently from hematopoietic stem cells. Indeed, fetal-derived immune cells are found in most organs and serous cavities of our body, where they reside throughout the entire lifespan. At the same time, there is a growing appreciation that pathologies manifesting in adulthood may be caused by adverse early life events, a concept known as “developmental origins of health and disease” (DOHaD). Yet, whether fetal-derived immune cells are mechanistically involved in DOHaD remains elusive. In this review, we summarize our knowledge of fetal hematopoiesis and its contribution to adult immune compartments, which results in a “layered immune system.” Based on their ontogeny, we argue that fetal-derived immune cells are prime transmitters of long-term consequences of prenatal adversities. In addition to increasing disease susceptibility, these may also directly cause inflammatory, degenerative, and metabolic disorders. We explore this notion for cells generated from erythro-myeloid progenitors (EMP) produced in the extra-embryonic yolk sac. Focusing on macrophages and mast cells, we present emerging evidence implicating them in lifelong disease by either somatic mutations or developmental programming events resulting from maternal and early environmental perturbations.

Usability digitaler Lesemedien

2021 ◽  
Author(s):  
Sibylle Kunz
Keyword(s):  
Print Media ◽  
Reference Model ◽  
Digital Reading ◽  
Wide Range ◽  
Range Of Functions ◽  
Multiple Case ◽  
International University ◽  
Usability Requirements ◽  
Conducting Research

Focusing on the reader’s benefit: Digital reading media offer a wide range of functions compared to print media: search functions, bookmarking, annotations, social reading, etc. However, they often fail to meet basic usability requirements, hindering reception and driving readers back to print media. This dissertation designs a reference model and derives a set of suitable methods from it with which to measure and improve the usability of digital reading media, including heuristic evaluation, user observation and eye-tracking, which it empirically tests on professional journal apps in a multiple case study. Sibylle Kunz is a professor of media computer science at IUBH International University, conducting research on digital reading media and their usability.

Psychosomatic Medicine: Past and Present Part II. Current State

1986 ◽  
Vol 31 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Z.J. Lipowski
Keyword(s):  
Psychosomatic Medicine ◽  
Current Phase ◽  
Clinical Aspects ◽  
Liaison Psychiatry ◽  
Psychological States ◽  
Psychophysiological Response ◽  
Current State ◽  
Wide Range ◽  
Health And Disease ◽  
Theory Research

The first historical phase of psychosomatic medicine was largely dominated by the psychoanalytic approach to research and theory. The current phase has shown a radical shift away from that approach. It has focused on the study of the physiological mechanisms mediating between psychological states and body functions in health and disease, and on the role of personally meaningful life events and psychophysiological responses to them in the etiology, course, and outcome of a wide range of physical illnesses. The concepts of psychosocial stress, psychophysiological response specificity, coping, adaptation, and social supports predominate in psychosomatic theory. Research has grown in scope, diversity, and methodological sophistication. Concurrently, clinical application and the psychosomatic approach to patient care has expanded in the form of consultation-liaison psychiatry and behavioural medicine. The author reviews the current state of the field in its scientific and clinical aspects, and offers definitions delineating its scope.

scholarly journals Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Shihua Wang ◽  
Xiaoxia Li ◽  
Robert Chunhua Zhao
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Length Distribution ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Therapeutic Effects ◽  
Wide Range

Mesenchymal stem cells (MSCs) possess great immunomodulatory capacity which lays the foundation for their therapeutic effects in a variety of diseases. Recently, toll-like receptors (TLR) have been shown to modulate MSC functions; however, the underlying molecular mechanisms are poorly understood. Emerging evidence suggests that long noncoding RNAs (lncRNAs) are an important class of regulators involved in a wide range of biological processes. To explore the potential involvement of lncRNAs in TLR stimulated MSCs, we performed a comprehensive lncRNA and mRNA profiling through microarray. 10.2% of lncRNAs (1733 out of 16967) and 15.1% of mRNA transcripts (1760 out of 11632) were significantly differentially expressed (absolute fold-change≥5 ,Pvalue≤0.05) in TLR3 stimulated MSCs. Furthermore, we characterized the differentially expressed lncRNAs through their classes and length distribution and correlated them with differentially expressed mRNA. Here, we are the first to determine genome-wide lncRNAs expression patterns in TLR3 stimulated MSCs by microarray and this work could provide a comprehensive framework of the transcriptome landscapes of TLR3 stimulated MSCs.

scholarly journals Transcript Profiling of MIKCc MADS-Box Genes Reveals Conserved and Novel Roles in Barley Inflorescence Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Hendrik N. J. Kuijer ◽  
Neil J. Shirley ◽  
Shi F. Khor ◽  
Jin Shi ◽  
Julian Schwerdt ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Floral Organ ◽  
Transcript Analysis ◽  
Mads Box ◽  
Mads Box Genes ◽  
Inflorescence Development ◽  
Abcde Model ◽  
Wide Range ◽  
Dicotyledonous Plants ◽  
Range Of Functions

MADS-box genes have a wide range of functions in plant reproductive development and grain production. The ABCDE model of floral organ development shows that MADS-box genes are central players in these events in dicotyledonous plants but the applicability of this model remains largely unknown in many grass crops. Here, we show that transcript analysis of all MIKCc MADS-box genes through barley (Hordeum vulgare L.) inflorescence development reveals co-expression groups that can be linked to developmental events. Thirty-four MIKCc MADS-box genes were identified in the barley genome and single-nucleotide polymorphism (SNP) scanning of 22,626 barley varieties revealed that the natural variation in the coding regions of these genes is low and the sequences have been extremely conserved during barley domestication. More detailed transcript analysis showed that MADS-box genes are generally expressed at key inflorescence developmental phases and across various floral organs in barley, as predicted by the ABCDE model. However, expression patterns of some MADS genes, for example HvMADS58 (AGAMOUS subfamily) and HvMADS34 (SEPALLATA subfamily), clearly deviate from predicted patterns. This places them outside the scope of the classical ABCDE model of floral development and demonstrates that the central tenet of antagonism between A- and C-class gene expression in the ABC model of other plants does not occur in barley. Co-expression across three correlation sets showed that specifically grouped members of the barley MIKCc MADS-box genes are likely to be involved in developmental events driving inflorescence meristem initiation, floral meristem identity and floral organ determination. Based on these observations, we propose a potential floral ABCDE working model in barley, where the classic model is generally upheld, but that also provides new insights into the role of MIKCc MADS-box genes in the developing barley inflorescence.

scholarly journals Traffic Management in Residential Areas: Case Study the South District of Luxor City, Egypt

2019 ◽  
Vol 3 (2) ◽  
pp. 220
Author(s):  
Riam Mohamed Elsagher Mahmod Elmorshedy
Keyword(s):  
Land Use ◽  
Traffic Management ◽  
Residential Areas ◽  
The South ◽  
Linear Network ◽  
Current State ◽  
Wide Range ◽  
Local Residents ◽  
Negative Impacts

Traffic management includes the implementation of a wide range of measures, devices and techniques based on a combination of parallel strategies, to improve safety and livability on streets by reducing the effect of vehicular traffic.The local residential streets have the intended traffic function of providing access to limited numbers of local residents. Traffic management for residential streets helps to preserve and enhance streets by minimizing the negative impacts of traffic and seeks to improve safety for pedestrians, cyclists, motorists and all other road users.The problem is that the planning of Egypt’s existing cities depended on grid and linear network streets withoutseparation land-use. Where the residential areas are always mixed land-use, (residential and commercial uses). This situation reflects on congestion residential areas; noisy, Traffic confusion, . . . . . . etc. So, the author aimed to create the traffic methodological for existing residential areas.This paper is applied in the south district of Luxor city, where the city’s authorities took some procedures formanaging traffic to no avail.This practical study includes: designing the traffic methodological framework, applying the framework at casestudy, and comparing the designed model with the current state of case study. The software programs used are SPSS and excel.The results show that the current state ignores some principles in comparison to the designed model. The important recommendations of research are: the designed model should be applied at case study, and the framework of traffic methodological for existing residential areas is applicable in different areas.

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