scholarly journals Evaluation of Anticonvulsant and Antioxidant Activity of Senna occidentalis Seeds Extracts

2019 ◽  
Vol 9 (2) ◽  
pp. 183-187
Author(s):  
Vijay Vikram Singh ◽  
Jainendra Jain ◽  
Arun Kumar Mishra
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anticonvulsant Activity ◽  
Future Research ◽  
Maximal Electroshock ◽  
Formulation Development ◽  
Dpph Method ◽  
Dose Dependent Fashion ◽  
Seizure Model ◽  
Dose Dependent

Aim: The aim of present work was to determine the anticonvulsant and antioxidant activity of Senna occidentalis L. ethanolic seed extract by different mod­els. Methods: For evaluation of anticonvulsant activity, Pentylenetetrazole (PTZ) seizure model and Maximal electroshock (MES) seizure model were used. For antioxidant activity, (1, 1-diphenyl - 2-picryl hydrazine (DPPH) and hydrogen peroxide (H2O2) method were used. Results: The finding suggested that the ethanolic extract (EAE) of Senna occidentalis in the dose 400 mg/kg body weight posses potent anticonvulsant activity. The EAE showed anticonvulsant action in dose dependent fashion. It was observed that upon increasing the concentration of extract, it showed reduced absorbance and increased free radical inhibition, and when comparison was made with Ascorbic acid, it showed marked antioxidant property in DPPH as well as H2O2 method. The IC50 of Ascorbic acid and EAE by DPPH method were found to be 14.56 and 14.8 respectively whereas the IC50 of Ascorbic acid and EAE by H2O2 method were found that 14.3and 14.8 respectively. Conclusion: The results of the present study concluded hat the EAE of Senna occidentalis L. possesses significant antioxidant and anticonvulsant activity. The activity was in dose dependent fashion. This study will assist in future research associated with formulation development of seeds of Senna occidentalis L. Keyword: Senna occidentalis L., Anticonvulsant, Antioxidant, DPPH model

scholarly journals Investigation on Anticataract Activity of Triphala Ghrita

2011 ◽  
Vol 8 (3) ◽  
pp. 1438-1443
Author(s):  
K. N. Mahajan ◽  
A. K. Singhai ◽  
G. P. Vadnere
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ellagic Acid ◽  
Stage Iv ◽  
Stage Iii ◽  
Albino Rats ◽  
Significant Protection ◽  
Preliminary Study ◽  
Group B ◽  
Dose Dependent

Lab prepared Triphala ghrita was studied for its possible anticataract activity on galactose induced cataract in Swiss albino rats. Administration of Triphala ghrita at a dose of 216 mg/200 g, 1080 mg/200 g and 2160 mg/200 g of rat orally offered significant dose dependent protection against galactose induced cataract and delayed the onset and progression of cataract. It was seen that the dose of 1080 mg of Triphala ghrita did not show stage II cataract in 14 days and stage III in 21 days. It did not show even stage III and stage IV cataract after 30 days whereas group B and group C showed stage III and stage IV cataracts. Dose of 2160 mg did not showed stage IV cataract after 30 days but showed stage III cataract. Dose of 1080 mg of Triphala ghrita offered significant protection against delaying the onset and progression of cataract in comparison to other doses. This effect may be attributed to the antioxidant activity of gallic acid, ellagic acid and ascorbic acid which shows their presence in Triphala ghrita. This preliminary study was encouraging but further studies are required to extrapolate the clinical usefulness of this formulation.

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals Amaranth Leaf Extract Protects Against Hydrogen Peroxide Induced Oxidative Stress in Drosophila Melanogaster

2021 ◽  
Author(s):  
Johnmark Ndinawe ◽  
Hellen W. Kinyi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Dependent Manner ◽  
Polyphenol Compounds ◽  
Dose Dependent

Abstract ObjectiveAmaranths leaves are rich in ascorbic acid and polyphenol compounds which have antioxidant activity. The aim of this study was to evaluate their in vivo antioxidant activity. The effect of consumption of Amaranth leaf extract on in vivo antioxidant activity, catalase enzyme activity and H2O2 induced oxidative stress in Drosophila melanogaster flies was assessed.ResultsConsumption of Amaranth leaf extract was associated with increased survival on exposure to H202 in a dose dependent manner in Drosophila melanogaster flies.

scholarly journals The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

2019 ◽  
Vol 11 (2) ◽  
pp. 226-233
Author(s):  
Ikhsanti Maliya ◽  
Sri Darmanti ◽  
Sri Widodo Agung Suedy
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Developmental Stages ◽  
Ascorbic Acid Content ◽  
Total Phenol ◽  
Color Measurement ◽  
Total Phenol Content ◽  
Chlorophyll Contents ◽  
Syzygium Jambos ◽  
Dpph Method

Malabar plum [Syzygium jambos (L.) Alston.] is a tropical plant which is used as a medicinal plant, because it contains secondary metabolites, especially in the leaves. The different leaves developmental stages can affect physiological changes, especially metabolic processes, so it is suspected to affect the antioxidant content and activity. The objective of this research was to study the difference of leaves morphology, chlorophyll contents, antioxidant contents, and activity at the different leaves developmental stages. Samples were taken from Kaliboto Village, Purworejo, Central Java. The leaf color measurement was using colorimetry; determination of chlorophyll, carotenoids, ascorbic acid, and total phenol content is was using spectrophotometry; and antioxidant activity was using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Qualitative data were analyzed descriptively, while quantitative data were analyzed by ANOVA tests. The results showed that the higher level of leaves development, the higher pigment content, total phenol, and antioxidant activity ascorbic acid content in the mature leaves is lower when compared to the young and old leaves. The results of this research provide the information that can support the use of Malabar plum leaves in traditional medicinal activity and pharmaceutical industry, as well as basic information for plant breeding.

scholarly journals EVALUATION OF ANTICONVULSANT ACTIVITY OF CALLISTEMON CITRINUS (CURTIS) SKEELS (MYRTACEAE) VOLATILE OIL

2021 ◽  
Vol 2 (2) ◽  
pp. 117-125
Author(s):  
R. O. Imade ◽  
A. M. Akhigbemen ◽  
A. Uchendu ◽  
C. L. Onyeagoro
Keyword(s):  
Anticonvulsant Activity ◽  
Conventional Medicine ◽  
Volatile Oil ◽  
Positive Control ◽  
Maximal Electroshock ◽  
Acute Toxicity Test ◽  
Orthodox Medicine ◽  
Leg Extension ◽  
Model C ◽  
Dose Dependent

The use of medicinal plants is on the rise due to the increase of various diseases and shortcomings of orthodox medicine. For many ailments including convulsion, conventional medicine has not been able to find a lasting solution. This study was directed towards assessing the ethnomedicinal use of Callistemon citrinus leaves in the management of convulsion. The volatile oil of the leaves was extracted and an acute toxicity test was carried out following Lorke’s description. Maximal electroshock (MES), strychnine and pentylenetetrazol anticonvulsant methods were used. Separate groups of albino mice were given 200, 400 and 800 mg/kg doses of the volatile oil. Drug solutions; 30 mg/kg phenobarbitone for MES and 2 mg/kg diazepam for strychnine and pentylenetetrazol models were administered as a positive control. The start of tonic leg extension, duration and percentage mortality was recorded. Doses of 200 and 400 mg/kg significantly (P<0.05) inhibited seizure in the mice with scores of 40 % each in the MES model. There was a dose-dependent reduction in the duration of seizures with 68.47, 70.27 and 81.08 % reductions in the pentylenetetrazol model. No significant coverage was given in the strychnine model. C. citrinus oil protected the mice against pentylenetetrazol and maximal electroshock-induced convulsion hence could contribute to the medical treatment of epilepsy.

scholarly journals QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Deepak Jain ◽  
Meenakshi Jain ◽  
Anurekha Jain
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Test Protocol ◽  
Rauwolfia Serpentina ◽  
Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

A Comparative Study of Antioxidant Activity between Ethanolic and Aqueous Extract of Cardiospermum halicacabum

2021 ◽  
pp. 4715-4718
Author(s):  
Kumudhavalli M.V. ◽  
Nandhinipriya V.
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Hydroxyl Radical ◽  
Aqueous Extract ◽  
Research Work ◽  
Assay Method ◽  
Vitro Assay ◽  
Aqueous Solvent ◽  
Dpph Method

Cardiospermum halicacabum is conventionally used in the treatment of stiffness of limbs, chronic bronchitis, and snakebites. The whole plant of Cardiospermum halicacabum has been extracted using ethanol and aqueous solvent and estimation of their antioxidant activity done for predicted current research work. Both ethanol and aqueous extracts illustrate important antioxidant activity in the DPPH method, nitric oxide method, and hydroxyl radical-induced In-vitro assay method. The DPPH radical inhibition (%) was 53.13, 57.13, and 79.12 for EECH, AECH, and Standard ascorbic acid respectively. The Cardiospermum halicacabum extracts (EECH and AECH) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250μg/ml, showing 29.22% and 63.32% of nitric oxide inhibition, respectively. The EECH and AECH extracts (25-400μg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/HO system, when compared to that of ascorbic acid. The plant Cardiospermum halicacabum has been used to treat different ailments. The present study clearly showed that both the extracts viz. EECH and AECH of the plant possess significant antioxidant activity. Moreover, ethanolic extract was proved as more potent candidature as compared to aqueous extract. The molecular level mechanism is the future interest of the researchers to prove the traditional use of the plant.

Influence of ethacrynic acid on the anticonvulsant activity of conventional antiepileptic drugs in the mouse maximal electroshock seizure model

2010 ◽  
Vol 62 (5) ◽  
pp. 808-813 ◽  
Author(s):  
Krzysztof Łukawski ◽  
Grażyna Świderska ◽  
Jarogniew J. Łuszczki ◽  
Stanisław J. Czuczwar
Keyword(s):  
Antiepileptic Drugs ◽  
Anticonvulsant Activity ◽  
Ethacrynic Acid ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Seizure Model

scholarly journals Antioxidant potential study of some synthesized N-heterocycles

2009 ◽  
Vol 35 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sukanta Kumar Shaha ◽  
Foysal Aziz
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anticancer Activity ◽  
Heterocyclic Compounds ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Heating Method ◽  
Dpph Method ◽  
First Time

Nitrogen containing heterocyclic compounds such as oxindoles especially isatins and their derivatives have excellent biological properties such as anti-inflammatory, antimicrobial, anticancer activity. In addition, this is first time ever we would like to report the antioxidant properties of the said class of compounds. To determine such an important bioactivity a number of halogenated oxindoles 1-3, their diaza-biphenyl derivatives (4-6), ring extended biphenyl (7) and bis-amide (8) were synthesized by classical heating method. The antioxidant activity of all the synthesized compounds was screened by DPPH method with respect to ascorbic acid. In our present investigation some of the synthesized compounds (1, 2, 3, 5 and 6) were found to be active.Keywords: Antioxidant; Isatin; OxindoleOnline 12 August 2009DOI: 10.3329/bmrcb.v35i2.2564Bangladesh Med Res Counc Bull 2009; 35: 49-52 

Captopril potentiates the anticonvulsant activity of carbamazepine and lamotrigine in the mouse maximal electroshock seizure model

2010 ◽  
Vol 117 (10) ◽  
pp. 1161-1166 ◽  
Author(s):  
Krzysztof Łukawski ◽  
Tomasz Jakubus ◽  
Grzegorz Raszewski ◽  
Stanisław J. Czuczwar
Keyword(s):  
Anticonvulsant Activity ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Seizure Model
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