scholarly journals Daya Predasi Ikan Lemon (Labidochromis caeruleus) dan Ikan Kapiat (Barbonymus schwanenfeldii) Terhadap Larva Nyamuk Aedes aegypti

2021 ◽  
Vol 13 (1) ◽  
pp. 37-46
Author(s):  
Nathania Disa Ariesta Andriani ◽  
Hebert Adrianto ◽  
Arief Gunawan Darmanto

Dengue hemorrhagic fever (DHF) is a vector-borne disease that still happens every year in Indonesia. This disease is caused by the dengue virus and transmitted by the Aedes aegypti as its vector. One of the biological vector controls that can be implemented is using fish as a predator of mosquito larvae, such as using Electric Yellow Cichlid fish (Labidochromis caeruleus) and Tinfoil Barb fish (Barbonymus schwanenfeldii). This study aimed to determine the difference in predation capacities of Electric Yellow Cichlid and Tinfoil Barb on Ae. aegypti larvae. This research is quasiexperimental with a completely randomized design (CRD) with Post Test Only research design. One aquarium with three liters of water containing one fish is given 25 Ae. aegypti larvae. Testing of each fish species is carried out in five replications. Fish predation was observed every 30 seconds until all larvae were eaten. The result showed that Electric Yellow Cichlid could prey on 25 larvae in an averagetime of 5.7 minutes, while Tinfoil Barb spent 11.6 minutes. The results of the Friedman test showed that there was a significant time difference in preying time of Electric Yellow Cichlid and Tinfoil Barb. It’s concluded that Electric Yellow Cichlid was more effective as an Ae. aegypti larvae predator.

2020 ◽  
Vol 19 (2) ◽  
pp. 112-118
Author(s):  
Muhammad Rasyid Ridha ◽  
Budi Hairani ◽  
Gusti Meliyanie ◽  
Wulan Rasna Giri Sembiring ◽  
Abdullah Fadilly ◽  
...  

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water   ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami


2019 ◽  
Vol 11 (2) ◽  
pp. 87-93
Author(s):  
Wuri Artikasari ◽  
Emantis Rosa ◽  
Bambang Irawan

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.


Author(s):  
Nastiti Widoretno

Dengue Hemorrhagic Fever (DHF) is still one of the major public health problems in the world and Indonesia. There are several methods of mosquito vector control, one of them is the use of ovitrap and the addition of attractants which is a compound that may attract gravid mosquitoes to lay eggs. Some examples of attractants are hay infusion and sugar fermentation solution. This research aimed to compare effectiveness of hay infusion  and sugar fermentation solution as Aedes aegypti attractants. This research type was true experimental laboratory with complete randomized design which is divided into 3 groups of ovitrap containing aquades as control, 20% hay infusion and 20% sugar fermentation solution. Ovitraps with those attractants were put into a mosquito coop containing 25 gravid female mosquitoes. Mosquito’s eggs counting was done after 2 days with 9 times repetition. The data were then analyzed using Kruskal-Wallis and Mann-Whitney test. It was found that the difference of mosquito’s eggs number in the ovitrap containing the fermentation solution of sugar and hay infusion had a significance value of 0.000 (p> 0,05). It means that hay infusion is more effective compared with sugar fermentation. Keywords                   : DHF, ovitrap, attractant, hay infusion, sugar fermentation solution


Masker Medika ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 226-232
Author(s):  
Triana Oktaviani ◽  
Zairinayati Zairinayati

Latar Belakang: Aedes aegypti merupakan nyamuk yang dapat berperan sebagai vektor Demam Berdarah Dengue (DBD). Upaya promotif dan preventif menjadi mutlak dilakukan untuk memutus mata rantai penularan dengan membunuh larva nyamuk dengan larvasida alami menggunakan ekstrak daun sirih (Piper betle) sebagai insektisida alami. Tujuan dari penelitian: ini adalah untuk mengetahui perbedaan abate dan ekstrak daun sirih (Piper betle) dalam mematikan larva Aedes aegypti L instar III. Desain penelitian: ini bersifat eksperimen dengan menggunakan rancangan acak lengkap (RAL) yang terdiri dari 3 perlakuan 1 kontrol dan 8 kali pengulangan percobaan. Hasil Penelitian: dapat diketahui bahwa untuk penggunaan ekstrak daun sirih (Piper betle) dengan konsentrasi 5% dapat mematikan larva sebanyak 8 ekor, sedangkan konsentrasi 10% tidak dapat mematikan larva (0 larva) dan konsentrasi 15% dapat mematikan larva sebanyak 17 (85%) sedangkan untuk penggunaan abate dengan konsentrasi 5%, 10% dan 15% dapat mematikan larva sebanyak 160 larva (100%). Kesimpulan: bahwa abate mempunyai efektifitas lebih tinggi di banding dengan ekstrak daun sirh (Piper betle).     Background: Aedes aegypti is a mosquito that can act as a vector of dengue Hemorrhagic Fever (DHF). Promotove and preventive effort are absolutely necessary to break the chain to transmission by killing mosquito larvae with natural larvacides using betel leaf extract (Piper betle) as a natural insecticides. The purposes of this study: this was to determine the differences in abate and betel leaf extract (Piper batle) in kliing Aedes aegypti instar III larvae. Research methods this is an experimental study using a completely randomized design (CRD) consisting of 3 treatments 1 control and 8 repetitions of the experiment. The results: it can be seen that for the use of betel leaf extract(Piper betle) with a concentration cannot kill larvae in 17 (85%) whilke for the use of abate with a concentration of 5%, 10% and 15% can kill larvae of 160 (100%). Conclusion: that the abate has a higher effectiveness compared to sirih leaf extract.


2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Ana Windari ◽  
Mimatun Nasihah ◽  
Nur Lathifah Syakbanah

Dengue Hemorrhagic Fever (DHF) is an acute disease in the form of dengue virus infection which is spread through mosquito bites. The use of synthetic insecticides actually causes negative effects on the environment, so another alternative is needed, namely the use of bay leaf vegetable insecticides. The purpose of this study was to determine the effectiveness of bay leaf insecticide (Syzygium polyanthum) as an insecticide against the mortality of Aedes Aegypti mosquitoes with the liquid electric method. This study used a quasi-experimental (quasi-experimental) design with a completely randomized design (CRD) method where the experiment was conducted on 150 mosquitoes in 5 treatments and 3 replications. The results of the percentage of bay leaf solution (Syzgium polyanthum) which has the largest number of mosquito mortality at a concentration of 80 ml/200 ml of water with a mortality percentage of 60%. The result of probit LC50 analysis of bay leaf solution was 64,315 g/ml. The higher the concentration of the solution given, the greater the number of mosquito mortality. It is necessary to modify the right tools and methods to make a solution of bay leaf (Syzgium polyanthum) so that it is more effectively applied in the community.Keywords: bioinsecticide, bay leaf, liquid electric, mortality, Aedes aegypti


2017 ◽  
Vol 4 (2) ◽  
pp. 98
Author(s):  
Siti Mutmainah ◽  
Eko Prasetyo ◽  
Lilis Sugiarti

Beta Fish Predation Power and Guppies Against the Third Instar Larvae of Mosquitos as a Vector Control Efforts in Dengue Fever         Dengue Hemorrhagic Fever (DHF) disease was a disease caused by dengue virus infection remains a public health problem in Indonesia. The disease attacks all age groups every years. Effort to eradication DHF done by fulfill the predator of fish like cupang and guppy. The study to determined the difference of predation potential between betta and guppy toward the 3rd instar of larvae Aedes aegypti. This study was experimental with comparative study and one shoot chase study design. The population was larvae of Aedes aegypti mosquito 3rd instar with betta and guppy as many as 5. Every treatment used the larvae as many as 25. The treatment observe in 1, 2 , 4 and 6 hours the data analyze used the independent samples test. The results of independent samples test got the p value 0.000, proves there were the significantly difference of predation potential of betta and guppy toward the 3rd instar III of Larvae Aedes aegypti mosquito. Suggested to the society can used the predator fish to eradication the DHF disease. Keywords: The potential of predator fish Cupang (Betta sp.), Guppy (Poecilia reticulata), 3rd instar of larvae, Aedes aegypti. ABSTRAK        Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dan ditularkan oleh nyamuk Aedes aegypti yang masih menjadi masalah kesehatan masyarakat di Indonesia. Penyakit ini menyerang semua kelompok umur dan muncul setiap tahun. Pengendalian DBD dilakukan dengan memanfaatkan ikan seperti ikan cupang dan guppy. Penelitian ini bertujuan untuk mengetahui perbedaan  daya predasi  ikan cupang (Betta sp.) dan ikan guppy (Poecilia reticulata) terhadap larva instar III nyamuk Aedes aegypti. Jenis penelitian ini adalah  eksperimental bersifat komparatif dengan desain one shoot chase study. Subyek penelitian adalah larva nyamuk Aedes aegypti instar III, ikan cupang dan guppy masing-masing sebanyak 5 ekor. Setiap waktu pengamatan  menggunakan larva instar III sebanyak 25 ekor dan diulang sebanyak 5 kali. Pengamatan dilakukan selama 1, 2, 4 dan 6 jam. Analisis data menggunakan independent samples test. Hasil uji Independent Samples Test didapatkan nilai p value 0.000, membuktikan adanya perbedaan yang bermakna antara daya predator ikan cupang (Betta sp.) dengan ikan guppy (Poecilia reticulata) terhadap larva instar III nyamuk Aedes aegypti. Daya predator paling baik adalah  ikan cupang. Disarankan kepada masyarakat untuk memanfaatkan ikan predator dalam upaya mengendalikan penyakit Demam Berdarah.Kata Kunci: daya predator ikan cupang (Betta sp.), ikan guppy (Poecilia reticulata), larva instar III nyamuk Aedes aegypti.


2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae


2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.


2018 ◽  
Vol 12 (1) ◽  
pp. 31-51
Author(s):  
Aprilianto Bayu Arisandy ◽  
Agustina Widyasworo ◽  
Eddy Trijana Sudani

ABSTRACT The research was conducted at Mrs. Sripurwanti's farm in Rejotangan Village, Rejotangan Sub-district, Tulung Agung Regency. The research was conducted from 1 March to 1 April 2017. The aim of the research to determine the difference in the solution of Vitamin B Complex to the results of domestic chicken include hatchability, mortality and hatching weight. Research material using selected 225 eggs of chicken eggs and Vitamin B Complex as many as 30 grains. The research method used is experiments with Completely Randomized Design (RAL). The study was divided into 5 treatments and 5 replications per repeat consisting of 9 eggs. Treatment consists of the addition of Vitamin B complex as much as 0, 45 mg, 90 mg, 135 mg, and 180 mg The results are expected can provide information about alternative moisture enhancer ingredients namely Vitamin B Komplek. The results showed that vitamin B complex was not significant (P> 0.05) to hatchability, live eggs and die 2nd candling , mortality, and hatching weight. Vitamin B complex has significant effect (P <0,05) on hatchability and mortality based on candling to 2. Result of the research can be concluded that giving Vitamin B Complex as much as 45 mg deserves to be additive addition of humidity because it can increase the percentage of hatchability by 15%


2019 ◽  
Vol 4 (1) ◽  
pp. 538-546
Author(s):  
Sifa' Fauziyani ◽  
Fahrizal Fahrizal ◽  
Anshar Patria

Abstrak. Keumamah (ikan kayu) merupakan makanan tradisional khas Aceh yang dibuat dari ikan tuna.Tetapi ikan kayu (keumamah) tersebut tidak dikemas, yang menyebabkan ikan kayu (keumamah) cepat terserangbakteri dan pertumbuhan jamur jenis Penicillium sp. Untuk itu diperlukan alternatif pengawet alami dalampenelitian ini yaitu kitosan yang berasal dari cangkang kulit udang sebagai bahan dasar pembuatan ediblecoating. Tujuan penelitian ini adalah untuk mengetahui konsentrasi kitosan terbaik sebagai edible coating untukmemperpanjang masa simpan keumamah pada suhu ruang. Penelitian ini menggunakan Rancangan AcakLengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama adalah konsentrasi Kitosan (K) yaitu K0 = 0%kontrol, K2 = 1% Kitosan, K2 = 2% kitosan. Faktor kedua adalah lama penimpanan (P) yaitu P1 =4 minggu,P2= 6 minggu, dan P3=8 minggu. Nilai a w yang dihasilkan pada penelitian ini 0,65, kadar air 4,35%, totalmikroba 7,51 cfu ml/gram, TBA 0,25 manoldehida/gram, kadar protein 36,79%, dan organoleptik tekstur 2,48.Perbedaan konsentrasi kitosan pada keumamah berpengaruh sangat nyata terhadap a w , dan lama penyimpananberpengaruh nyata terhadap kadar air, total mikroba, Thiobarbituric Acid (TBA), analisis protein, danorganoleptik deskriptif aroma . Berpengaruh nyata terhadap nilai organoleptik warna. Dan tidak berpengaruhnyata terhadap uji organoletik tekstur serta tidak terdapatnya bakteri E.coli pada keumamah berkonsentrasikitosan. Pada keumamah konsentrasi terbaik di tunjukkan pada konsentrasi 1% dan 2%.Abstract. Keumamah (wooden fish) is Acehnese traditional food made from tuna fish. But, tradisionalkeumamah was unpackaged, this caused easily attacked by pest and growth penicillium sp. Natural preservativeneeded in this study is chitosan shrimps skin shells as primary substance to make edible coating. The objective ofthis study is to obtain the best chitosan concretations as edible coating to extend keumamah shelf life in roomtemperature. This study used completely randomized design consisting of 2 factors. First factor is chitosanconsentration (K) K0=0% control, K1=1% Chitosan, K2=2% Chitosan. Second factor is long storage (P) P1= 4weaks, P2= 6 weaks, P3= 8 weaks. A w produced in this study is 0,65, moisture 4,35, total plate count 7,51, TBA,0,25 manoldehida/gram, Protein 36,79%, and organoleptic test texture 2,48. The difference of chitosanconsentration in keumamah influenced significantly to a w , and long storage influenced to moisture, TotalPlate Count (TPC), and fragrance and texture organoleptic and not influenced to Thiobarbituric Acid (TBA) andE. coli bacteria not found in keumamah coated by edible coating.


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