scholarly journals Trypanosoma vivax in Dairy Cattle

2018 ◽  
Vol 46 ◽  
pp. 5
Author(s):  
Sabrina Thabla Pereira Lopes ◽  
Bruno Da Silva Prado ◽  
Gustavo Henrique Chaves Martins ◽  
Hiran Esmeraldo Albuquerque Beserra ◽  
Marcos Antônio Celestino de Souza Filho ◽  
...  
Keyword(s):  
Clinical Condition ◽  
Clinical Signs ◽  
Dairy Farm ◽  
Chronic Phase ◽  
Dairy Herd ◽  
Normocytic Anemia ◽  
High Morbidity ◽  
Trypanosoma Vivax ◽  
Serological Tests ◽  
Epidemiological Situation

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitologicalform and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarian’s technicalvisit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.Discussion: Blood trypomastigote forms of Trypanosoma vivax were visualized on several slides of the animal smear and all the morphological structures of the parasite found were clearly seen under microscopyas described in the literature. The hematological alterations observed were normochromic normocytic anemia, thrombocytopenia, leukocytosis due tolymphocytosis, monocytosis and eosinophilia. The anemia and leukocytosis clinical condition found in the specific animal is commonly found in bovines naturally infected by trypanosomiasis in the chronic phase of the disease. The results of the serum biochemistry revealed decreased blood glucose and increased renal and hepatic parameters, as well as the inversion of the albumin-globulin ratio. Similar laboratory results were also described previously. The animal had a good body score, a good diet and showed no clinical signs of the disease. A good nutrition may have controlled the T. vivax parasitaemia, avoiding the characterization of the clinical condition. This fact can be attributed to the differences in pathogenicity of the parasite and/or susceptibility of a particular host. In the area of the farm where the cattle were housed the presence ofseveral tabanids was noticed and the increase of these insects in the environment is considered a risk factor, predisposing, even, the occurrence of new outbreaks. The epidemiological situation of the disease in Brazil is described, for the most part, by reports of outbreaks or specific events, revealing the lack of more consistent studies. With this result it is knownthat trypanosomiasis exists in the dairy herd of the state of Piauí, being important to carry out new work to diagnose the epidemiological situation of the disease within the productive context of our region.Keywords: bovine, dairy herd, trypanosomiasis.

scholarly journals Canine ehrlichiosis: clinical, hematological, serological and molecular aspects

2008 ◽  
Vol 38 (3) ◽  
pp. 766-770 ◽  
Author(s):  
Andréa Cristina Higa Nakaghi ◽  
Rosangela Zacarias Machado ◽  
Mirela Tinucci Costa ◽  
Marcos Rogério André ◽  
Cristiane Divan Baldani
Keyword(s):  
Direct Detection ◽  
Clinical Signs ◽  
Chronic Phase ◽  
Antibody Test ◽  
Acute Stage ◽  
Detection Methods ◽  
Serological Tests ◽  
Humoral Immune ◽  
Canine Ehrlichiosis ◽  
Dot Elisa

The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques it was concluded that serology and nPCR are the most suitable tests to confirm the diagnosis of canine ehrlichiosis, however it should be always treated as a complementary data to clinical and hematological evaluation. Serology has an important role in the subclinical and in the chronic phase, nPCR is recommended in the acute stage, and, especially, to identify the ehrlichia specie.

scholarly journals Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax

2016 ◽  
Vol 25 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Otavio Luiz Fidelis Junior ◽  
Paulo Henrique Sampaio ◽  
Rosangela Zacarias Machado ◽  
Marcos Rogério André ◽  
Luiz Carlos Marques ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acute Phase ◽  
Blood Cells ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Chronic Phase ◽  
Diagnostic Value ◽  
Biochemical Changes ◽  
Severe Neutropenia ◽  
Trypanosoma Vivax

Abstract Infections by Trypanosoma vivax cause great losses to livestock in Africa and Central and South Americas. Outbreaks due this parasite have been occurred with increasing frequency in Brazil. Knowledge of changes caused byT. vivax during the course of this disease can be of great diagnostic value. Thus, clinical signs, parasitemia, hematologic and biochemical changes of cattle experimentally infected by this hemoparasite were evaluated. Two distinct phases were verified during the infection – an acute phase where circulating parasites were seen and then a chronic phase where fluctuations in parasitemia were detected including aparasitemic periods. A constant reduction in erythrocytes, hemoglobin and packed cell volume (PVC) were observed. White blood cells (WBC) showed pronounced changes such as severe neutropenia and lymphopenia during the acute phase of the illness. Decreases in cholesterol, albumin, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and increases in glucose, globulin, protein, and alkaline phosphatase (ALP) were observed. The “Lins” isolate of T. vivax showed pathogenicity for cattle, and intense parasitemia was detected in the early stages of infection. Circulating parasites were detected for about two months. The most evident laboratory abnormalities were found in WBC parameters, including thrombocytopenia.

scholarly journals Brain Abscess Caused by Nocardia: Case Report and Literature Review

2020 ◽  
Author(s):  
Guilherme Finger ◽  
Maria Eduarda Conte Gripa ◽  
Tiago Paczko Bozko Cecchini ◽  
Tobias Ludwig do Nascimento
Keyword(s):  
Case Report ◽  
Antibiotic Therapy ◽  
Brain Abscess ◽  
Clinical Signs ◽  
Occipital Lobe ◽  
High Morbidity ◽  
Rare Clinical Entity ◽  
Brain Abscesses

AbstractNocardia brain abscess is a rare clinical entity, accounting for 2% of all brain abscesses, associated with high morbidity and a mortality rate 3 times higher than brain abscesses caused by other bacteria. Proper investigation and treatment, characterized by a long-term antibiotic therapy, play an important role on the outcome of the patient. The authors describe a case of a patient without neurological comorbidities who developed clinical signs of right occipital lobe impairment and seizures, whose investigation demonstrated brain abscess caused by Nocardia spp. The patient was treated surgically followed by antibiotic therapy with a great outcome after 1 year of follow-up.

Reactivity and Specificity of Trichinella spiralis Fractions in Cutaneous and Serological Tests

1977 ◽  
Vol 6 (3) ◽  
pp. 274-279
Author(s):  
Omar O. Barriga
Keyword(s):  
Guinea Pigs ◽  
Trichinella Spiralis ◽  
Chronic Phase ◽  
Epidemiological Studies ◽  
Cross Reactivity ◽  
Serological Tests ◽  
Specific Diagnosis ◽  
Diethylaminoethyl Cellulose ◽  
Cutaneous Reactions ◽  
Healthy Persons

Six diethylaminoethyl-cellulose fractions of a larval Trichinella spiralis extract, an Ascaris suum extract, and a nonrelated protein were used for cutaneous tests in guinea pigs with 8-, 14-, and 73-day-old T. spiralis infections, in guinea pigs with 13-day-old A. suum infections, and in normal guinea pigs. A selected T. spiralis fraction was used in hemagglutination (HA) tests with sera of 8 T. spiralis -infected rabbits, 41 sera of trichinellosis patients positive by bentonite agglutination tests, and 50 sera of clinically healthy persons. Immediate-type cutaneous reactions revealed extensive cross-reactivity between both parasites, although the establishment of conventional limits for considering a reaction positive allowed the specific diagnosis of acute or chronic trichinellosis with different fractions. Delayed-type reactions were specific with all fractions except one, and different fractions reacted during either the acute or the chronic phase of trichinellosis. HA detected anti- Trichinella antibodies in all the rabbits 9 to 10 days postinfection, in all trichinellosis patients, and in none of the healthy people. Correlation between HA and bentonite agglutination titers and other considerations suggest that HA with the selected fraction detects early antibodies. HA inhibition tests with A. suum extract suggest lack of HA cross-reactivity between the A. suum - and T. spiralis -selected fractions. The use of different fractions in diverse tests for clinical or epidemiological studies is suggested.

Markers of the early stage of rheumatoid arthritis

2016 ◽  
Vol 51 (4) ◽  
pp. 305-314
Author(s):  
Beata Polińska ◽  
Joanna Matowicka-Karna ◽  
Halina Kemona
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Population ◽  
Early Stage ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Unknown Etiology ◽  
Serological Tests ◽  
Citrullinated Peptide ◽  
Peptide Antibodies

Rheumatoid arthritis (RA) is a chronic, autoimmune connective tissue disease of unknown etiology. RA affects about 1% of the human population, women suffer three times more often than men, with the peak incidence between the age of 40 to 50. The up-to-date criteria from 2010 for the diagnosis of RA include: occurrence and duration of clinical signs, indicators of inflammation and serological tests. Neopterin, a protein released by macrophages, is a sensitive indicator of inflammation and the severity of RA. Regarding the serological tests, anti-cyclic citrullinated peptide antibodies represent a well-known marker with the specificity for RA of about 98%. The antibodies may be present in the serum of patients even a few years before the first clinical signs of the disease, heralding erosive changes in the joints and more severe course of RA. The literature also contains reports about autoantibodies anti-CarP and anti-Sa/ anti-MCV, which may occur in people with pain and swelling of joints and precede full-blown development of RA as well as reflect disease activity. Serological diagnosis of RA may be supported by some genetic tests based on PCR for detecting mutations e.g. C1858T in the PNPN22 gene. In turn, the quantitative analysis of different classes of miRNAs seems justified in order to better classify patients showing symptoms of RA. Further studies are needed that take into account the role of different markers in the development of RA, and confirm the high sensitivity and specificity of these markers in the diagnosis of the disease.

scholarly journals Acute Kidney Injury and Acute Liver Failure in Leptospira Infection and Weil’s Syndrome

2020 ◽  
Vol 4 (2) ◽  
pp. 21-28
Author(s):  
Jonathan S Chávez-Iñiguez ◽  
Jose Said Cabrera-Aguilar ◽  
Guillermo Garcia-Garcia ◽  
Juan Armendáriz-Borunda
Keyword(s):  
Acute Kidney Injury ◽  
Clinical Presentation ◽  
Clinical Signs ◽  
Kidney Injury ◽  
Organ Damage ◽  
Multiple Organ ◽  
Serological Tests ◽  
Microbiological Criteria ◽  
Sanitary Conditions ◽  
The Impact

Leptospirosis is considered a zoonosis acquired predominantly from contaminated surfaces and water, more commonly in emerging countries with limited sanitary conditions. Leptospira in the host unleashes an immune response that explains the symptoms and clinical signs; once it reaches the kidney and liver tissue, it can manifest with alterations that lead to acute and chronic diseases in both organs. Weil’s syndrome is the best known clinical manifestation with jaundice and acute kidney injury that could lead to multiple organ failure and death. For its diagnosis, there are simplified scores such as the SPiRO score, the microbiological criteria by microscopy or serological tests; the treatment focuses on antibiotics and, if necessary, provides organic support until the infection is curtailed. The purpose of this review was to address the impact of Lep-tospira infection on the kidney and liver, the mechanisms of organ damage, the clinical presentation, and diagnosis and management of this disease.

scholarly journals Clinico-epidemiological features of Japanese encephalitis patients hospitalized in a tertiary care center

2018 ◽  
Vol 5 (10) ◽  
pp. 4548
Author(s):  
Abu Hasan Sarkar ◽  
Bishnu Ram Das
Keyword(s):  
Japanese Encephalitis ◽  
Care Center ◽  
Tertiary Care ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Complete Recovery ◽  
High Morbidity ◽  
Medical College ◽  
Clinical Signs And Symptoms ◽  
One Year

Background: Japanese encephalitis (JE) is of particular interest as it has a high morbidity and mortality. Neurological sequale is the most dreaded damage caused by JE. It is a preventable disease with specific interventions. The objective of the study was to study the demography, clinical profile and outcome of patients with Japanese Encephalitis admitted to the wards of Internal Medicine and Pediatrics at Jorhat Medical College Hospital.Methods: Hospital based observational study for one year in Jorhat Medical College, Jorhat, Assam.Results: The mean age for JE was 32.25±27 years for male, 27.47±22 years for female and 29.94±24 years overall. Assessment of clinical signs and symptoms showed that fever and change in mental status were present in 100% of JE cases followed by neck rigidity in 79.3% and headache in 68.9%. 44.8% of JE cases had history of seizure, 37.9% had vomiting, 34.5% had irritability, 13.8% were unconscious. The peak of JE incidence occurred in the month of July (77.6%). Complete recovery was seen in 39.2%, followed by death in 32.6% and recovery with neurological sequalae in 28.2% at the time of discharge.Conclusions: Vigorous awareness activities should be carried out to sensitize people on prevention of JE. 

scholarly journals Experimental Chemotherapy for Chagas Disease: A Morphological, Biochemical, and Proteomic Overview of Potential Trypanosoma cruzi Targets of Amidines Derivatives and Naphthoquinones

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Solange L. de Castro ◽  
Denise G. J. Batista ◽  
Marcos M. Batista ◽  
Wanderson Batista ◽  
Anissa Daliry ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Chronic Phase ◽  
Public Health Problem ◽  
Term Therapy ◽  
High Morbidity ◽  
Synthetic Compounds ◽  
Natural And Synthetic

Chagas disease (CD), caused by Trypanosoma cruzi, affects approximately eight million individuals in Latin America and is emerging in nonendemic areas due to the globalisation of immigration and nonvectorial transmission routes. Although CD represents an important public health problem, resulting in high morbidity and considerable mortality rates, few investments have been allocated towards developing novel anti-T. cruzi agents. The available therapy for CD is based on two nitro derivatives (benznidazole (Bz) and nifurtimox (Nf)) developed more than four decades ago. Both are far from ideal due to substantial secondary side effects, limited efficacy against different parasite isolates, long-term therapy, and their well-known poor activity in the late chronic phase. These drawbacks justify the urgent need to identify better drugs to treat chagasic patients. Although several classes of natural and synthetic compounds have been reported to act in vitro and in vivo on T. cruzi, since the introduction of Bz and Nf, only a few drugs, such as allopurinol and a few sterol inhibitors, have moved to clinical trials. This reflects, at least in part, the absence of well-established universal protocols to screen and compare drug activity. In addition, a large number of in vitro studies have been conducted using only epimastigotes and trypomastigotes instead of evaluating compounds' activities against intracellular amastigotes, which are the reproductive forms in the vertebrate host and are thus an important determinant in the selection and identification of effective compounds for further in vivo analysis. In addition, due to pharmacokinetics and absorption, distribution, metabolism, and excretion characteristics, several compounds that were promising in vitro have not been as effective as Nf or Bz in animal models of T. cruzi infection. In the last two decades, our team has collaborated with different medicinal chemistry groups to develop preclinical studies for CD and investigate the in vitro and in vivo efficacy, toxicity, selectivity, and parasite targets of different classes of natural and synthetic compounds. Some of these results will be briefly presented, focusing primarily on diamidines and related compounds and naphthoquinone derivatives that showed the most promising efficacy against T. cruzi.

Key issues in dairy herd health management

2021 ◽  
pp. 3-26
Author(s):  
John Remnant ◽  
◽  
James Breen ◽  
Peter Down ◽  
Chris Hudson ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Business Models ◽  
Health Management ◽  
Dairy Farm ◽  
Dairy Herd ◽  
Population Level ◽  
Holistic Approach ◽  
Herd Health ◽  
Health Target ◽  
Cow Health

Dairy herd health management benefits dairy farmers, the environment, dairy cows and citizens. It is an important part of modern dairy farm veterinary care. Dairy herd health management is assessing, monitoring and improving the health of dairy cows at a population level. Good herd health management takes a holistic approach and is ongoing and cyclical. All members of the dairy farm team and their advisors are involved, decisions are informed by data generated by the herd. These data may come from numerous sources. The data are processed and analysed to monitor cow health, target investigations and evaluate progress. To make lasting change on farms, advisors must communicate appropriately with farm managers to understand behaviour and motivate change. This chapter reviews these aspects of dairy herd health management, giving practical suggestions on how to get started, how to incorporate herd health management into business models and how to maintain momentum.

Anemia and Blood Transfusion

2019 ◽  
pp. 319-324
Author(s):  
Joy D. Hughes ◽  
Mariela Rivera ◽  
Myung S. Park
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Blood Transfusion ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Clinical Signs ◽  
Mortality Rates ◽  
Hemoglobin Level ◽  
High Morbidity ◽  
Active Hemorrhage

Critically ill patients commonly present with anemia, defined as a hemoglobin level less than 13.0 g/dL in men and less than 11.6 g/dL in women or as clinical signs of bleeding, including tachycardia and low urine output with active hemorrhage. Anemia is common, occurring in up to a third of critically ill patients, and is associated with high morbidity and mortality rates, particularly in patients with central nervous system injuries and disease. The causes of anemia can vary from chronic conditions such as kidney disease or malnutrition to acute conditions such as bleeding or consumptive coagulopathy.

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