COMPARISON OF THE EFFECTS OF TWO DIFFERENT ANTIOXIDANT COMBINATIONS ON SPERM PARAMETERS

Male infertility may be the result of some identifiable conditions (varicocele, cryptorchidism, hypogonadism, and genetic abnormalities), but there is no identified cause in 30-40% of infertile men. Studies show that the use of antioxidants has a beneficial effect on fertility, especially on sperm quality, and therefore recommended as a potentially effective treatment for male infertility. However, it is argued that this treatment is not based on sufficient evidence and has no effect on the rate of healthy pregnancy. Aim: In this study, two different antioxidant combinations with different doses and contents were evaluated in terms of their effect on sperm parameters. Materials and Methods: A total of 122 patients diagnosed with idiopathic infertility were enrolled in our multicenter study. The patients were divided into two different groups: The first group used Proxeed Plus sachets 2x1 and the second group used Carniact Duo tablets 2x1 for six months. The total semen volume, total sperm count and concentration, motile sperm count, and morphological findings of the patients were compared at the end of the six months. The mean age of the patients participating in the study was 30.8 ± 6.05 years. No significant difference was found between the two groups in terms of baseline sperm count. Results: There was a significant difference between the baseline and sixth-month values of the patients using both combinations. However, no significant statistical difference was found between the groups according to the sixth-month data. Conclusion: The combinations of both antioxidants had a positive effect on sperm parameters, and the use of different doses and contents had a similar effect. Keywords: Antioxidants, Infertility, Vitamins, Spermiogram,

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Improvement in Sperm Parameters With Traditional Iranian Remedy

Journal of Evidence-Based Complementary & Alternative Medicine ◽

10.1177/2156587215627536 ◽

2016 ◽

Vol 22 (2) ◽

pp. 223-226 ◽

Cited By ~ 1

Author(s):

Farnaz Sohrabvand ◽

Somaye Mahroozade ◽

Sodabe Bioos ◽

Seyed Mohammad Nazari ◽

Fataneh Hashem Dabaghian

Keyword(s):

Male Infertility ◽

Traditional Medicine ◽

Assisted Reproductive Technology ◽

Intracytoplasmic Sperm Injection ◽

Sperm Quality ◽

Intrauterine Insemination ◽

Sperm Count ◽

Reproductive Technology ◽

Sperm Parameters ◽

Medicinal Treatment

Introduction. Idiopathic male infertility is a global problem with almost no definite medicinal treatment. Most patients have to go through intrauterine insemination or assisted reproductive technology for achieving fertility. Unfortunately, success rates are low in cases with very low sperm count. Therefore it seems that improvement in sperm quality can have beneficial effects on assisted reproductive technology outcome. Case Report. A 39-year-old man with history of infertility for 6 years was referred to the traditional medicine clinic with a recurrent unsuccessful intracytoplasmic sperm injection trial. His sperm analysis showed severe oligoasthenoteratozoospermia. After taking a traditional remedy he had a remarkable improvement in his sperm parameters, which led to the formation of 8 embryos in the following intracytoplasmic sperm injection cycle. Conclusion. Traditional medicine presents various food and remedy options for treating male infertility. It seems that combination therapy can be beneficial in obtaining better results in treatment of male idiopathic infertility.

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The effects of two different antioxidant combinations on sperm parameters

Urologia Journal ◽

10.1177/03915603211049888 ◽

2021 ◽

pp. 039156032110498

Author(s):

Mehmet Yaris ◽

Nebil Akdogan ◽

Murat Öztürk ◽

Aliseydi Bozkurt ◽

Mehmet Karabakan

Keyword(s):

Folic Acid ◽

Vitamin C ◽

Coenzyme Q10 ◽

Positive Impact ◽

Sperm Concentration ◽

Sperm Parameters ◽

Sufficient Evidence ◽

Significant Difference ◽

Positive Effect ◽

Different Doses

Background: Studies demonstrate that getting antioxidants in the course of treatment has a positive impact beneficial effect on fertility, especially on the quality of sperm. Because of that reason antioxidants are recommended as a potentially influential treatment for infertility in men. However, it is argued that this treatment is not based on sufficient evidence and has no effect on the rate of healthy pregnancy. Objective: In this study, two different antioxidant combinations with different doses and contents were evaluated in terms of their effect on sperm parameters. Materials/methods: A total of 122 patients diagnosed with idiopathic infertility were enrolled in our multicenter study. The patients were divided into two different groups: The first group used a combination 2 × 1 sachet form (l-carnitine 1 g, acetyl-l-carnitine 0.5 g, fructose 1 g, citric acid 0.50 mg, selenium 50 µg, coenzyme Q10 20 mg, vitamin C 90 mg, zinc 10 mg, folic acid 200 µg, and vitamin B12 1.5 µg) and the second group used a combination tablets form 2 × 1 (l-carnitine 500 mg, selenium 50 µg, coenzyme Q10 20 mg, vitamin C 60 mg, zinc 15 mg, folic acid 400 µg, vitamin E, and ginseng 15 µg) for 6 months. The total semen volume, the total sperm number, sperm concentration, sperm motility, and lastly morphological findings of the patients were compared at the end of 6 months. Results: The mean age of the patients participating in the study was 30.8 ± 6.05 years. No significant difference was found between the two groups in terms of baseline sperm count. There was a significant difference between the baseline and sixth-month values of the patients using both combinations. However, no significant statistical difference was found between the groups according to the sixth-month data. The combinations of both antioxidants had a positive effect on sperm parameters, and the use of different doses and contents had a similar effect. Conclusion: Both antioxidants respectively had a positive effect on sperm parameters and also the use of different doses and contents had a similar effect.

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P–114 Correlation of GSTM1 polymorphism and oxidative stress with male infertility

Human Reproduction ◽

10.1093/humrep/deab130.113 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

D Mavrogianni ◽

A Voitse ◽

L Evgeni ◽

S Stavros ◽

P Drakakis

Keyword(s):

Oxidative Stress ◽

Male Infertility ◽

Sperm Count ◽

Sperm Parameters ◽

Control Group ◽

Genetic Profile ◽

Oxidative Potential ◽

Increased Risk ◽

Significant Difference ◽

Gstm1 Polymorphism

Abstract Study question Is GSTM1 polymorphism a putative biomarker of male infertility. Is there a possible correlation between GSTM1 presence, oxidative stress and male infertility? Summary answer A possible correlation may be established between GSTM1 polymorphism, and sperm mobility and morphology. Additionally oxidative potential may also be associated with fertility. What is known already Approximately 7% of men worldwide are affected by male infertility, which contributes to 40–50% of all infertility cases. Basic sperm analysis remains the main procedure of diagnosing male infertility, although there is still doubt whether it provides accurate clinical outcomes More accurate tests are essential for the diagnosis of male infertility. Oxidative stress is involved in the etiology of male infertility, with 30% to 80% of infertile men having increased levels of sperm free radicals. Recent research has shown that oxidative stress when combined with GSTM1-null genotype negatively affected the sperm quality of infertility group compared to the control group. Study design, size, duration Ninety semen samples were collected and divided into 2 groups. The study group consisted of sperm samples from 51 men identified as infertile according to WHO guidelines(case group).Sperm samples from 39 men with normal sperm count parameters (control group) were used for the control group. Participants/materials, setting, methods For all samples a sperm diagram was performed. and DNA was extracted. Polymerase chain reaction with specific for GSTM1 gene primers followed by agarose electrophoresis was applied to detect the presence of polymorphism. The MiOXSYS method was used to detect the oxidative potential. Main results and the role of chance This study shows that in the control group the presence of polymorphism was associated with a reduced number of immobile sperm cells (p = 0,035) while it appears to affect the normal morphology of the sperm(p = 0,042). In the infertility group the presence of the gene was significantly correlated with age(p = 0.046). No statistically significant difference was observed for the presence of the polymorphism between the 2 groups.In addition, we investigated the effect of oxidative potential with the MiOXSYS system and its relationship with sperm parameters. It was found that the two groups differed significantly when measuring oxidative potential, and that oxidoreduction potential affects sperm concentration/ml, total sperm count, type B motility and viscosity in the infertile male group. Limitations, reasons for caution A larger sample size could increase the accuracy of the results. Wider implications of the findings: Studying the relation of the oxidative stress with sperm parameters may lead to the establishment of a genetic profile of increased risk of infertility, which would be of major importance especially in cases of idiopathic infertility. Trial registration number Not applicable

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Gentamicin and amikacin adversely affect male infertility indicated by pharmacological, andrological and pathological evidence

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20200167 ◽

2020 ◽

Vol 9 (2) ◽

pp. 218

Author(s):

Hozaifa K. Elsawah ◽

Mohamed M. Kandiel ◽

Aziza A. Amin ◽

Haitham M. Mokhimar ◽

AbuBakr M. El Mahmoudy

Keyword(s):

Male Infertility ◽

Therapeutic Dose ◽

Sperm Count ◽

Serum Testosterone ◽

Reproductive Organs ◽

Serum Testosterone Level ◽

Double Dose ◽

Medication History ◽

The Third ◽

Significant Difference

Background: Many drugs are implicated in male infertility and screening for medication history is an important for diagnosis and treatment of the problem. The aim is to study amikacin effect on male reproductive system in comparison to gentamicin.Methods: Twenty-five male wister rats weighted 220±20 gm and aged 8 weeks were randomly divided into five groups of five. The first group received gentamicin in dose 18.25 mg/kg/day once daily (OD) (therapeutic dose). The second group received gentamicin with double dose of the first group. The third group received amikacin in dose 54.75 mg/kg/day OD (therapeutic dose). The Fourth group received amikacin with double dose of the third group. However, the fifth group served as a control and received normal saline (NS) OD. All treatments were administered intraperitoneally (IP) for 14 days. On the 15th day, blood samples and reproductive organs were obtained from all animals. Testicular tissues were prepared for genetic testing and chemical and microscopical examination.Results: Amikacin and gentamicin negatively affected reproductive organs weights, sperm parameters, serum follicle stimulating hormone and luteinizing hormone (LH) level relative to control (p<0.05). However, serum testosterone level was only affected with gentamicin (p<0.05). A significant difference between gentamicin and amikacin was found in sperm count, testis and epididymis weights and serum testosterone and LH level (p<0.05). Testicular histopathological changes were also found with the two drugs with different degrees. Effects of both gentamicin and amikacin were dose-dependent.Conclusions: Both gentamicin and amikacin adversely affect andrological function that should be monitored and controlled during application of these drugs.

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MiR-125b-2 Knockout in Testis Are Associated with Targeting to PAP Gene, Mitochondrial Copy Number and Impaired Sperm Quality

10.20944/preprints201811.0563.v1 ◽

2018 ◽

Author(s):

Longlong Li ◽

Yanling Zhu ◽

Jiajie Sun ◽

Ting Chen ◽

Junyi Luo ◽

...

Keyword(s):

Male Infertility ◽

Copy Number ◽

Nuclear Dna ◽

Semen Quality ◽

Sperm Quality ◽

Sperm Count ◽

Male Reproduction ◽

Luciferase Assay ◽

Cell Secretion ◽

Mtdna Copy Number

microRNAs can cause male infertility by impacting sperm quality and impaired spermatogenesis. Since the miR-125 family plays an important role in regulating embryo development, but the function of miR-125b-2 in male reproduction remains unknown. In this study, we prepared a model of miR-125b knockout (KO) mice. Among the KO mice, the progeny test showed that litter sizes decreased significantly and the rate of non-parous females increased significantly (p<0.05). At the same time, the testosterone concentration increased significantly (p<0.01), with the remarkable decrease for estradiol (p<0.05). Moreover, sperm count decreased obviously (p<0.05) and the percentage of abnormal sperms increased significantly (p<0.01). Testicular transcriptome sequencing demonstrated that there were 173 up-regulated genes, including Papolb (PAP), and 151 down-regulated genes in KO mice compared with wild type (WT). KEGG and GO analysis showed many of these genes were involved in sperm mitochondrial metabolism and other cellular biological processes. Meanwhile, the sperm mitochondria DNA (mtDNA) copy number was increased significantly (p<0.01) in KO mice, but the integrity of mtDNA and nuclear DNA (nDNA) had no change. In the top 10 up-regulated genes, as a testis specific expressing gene, PAP can affect the process of spermatogenesis. Western blotting and Luciferase Assay validated that PAP was the target of miR-125b-5p. Intriguingly, we also found that both miR-125b and PAP were only highly expressed in germ cells (GC) instead of Leydig cells (LC) and Sertoli cells (SC), and miR-125b-5p could target PAP to regulate TM3 cell secretion of testosterone (p<0.05). Our study firstly demonstrated that miR-125b-2 could regulate testosterone secretion by directly targeting PAP and increase sperm mtDNA copy number to affect semen quality. The study indicated that miR-125b-2 had a positive influence on the reproductive performance of animal and could be a potential therapeutic target for male infertility.

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The Impact of Radiofrequency Waves on Male Infertility: A Systematic Review

Shiraz E-Medical Journal ◽

10.5812/semj.101741 ◽

2020 ◽

Vol In Press (In Press) ◽

Author(s):

Leili Darvish ◽

Azadeh Amraee ◽

Marjan Akhavan Amjadi ◽

Zahra Atarodi Kashani ◽

Masoumeh Ghazanfarpour ◽

...

Keyword(s):

Systematic Review ◽

Male Infertility ◽

Sperm Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Membrane Integrity ◽

Semen Parameters ◽

Cochrane Library ◽

Negative Effects ◽

The Impact

Context: As the use of cellphones and other electronic devices increases, concerns about the possible effect of radiofrequency waves on health are growing. Long-term use of the cellphone may have negative effects on sperm quality. Objectives: The purpose of this research was to examine men's infertility due to the effect of radiofrequency waves. Methods: In this systematic review, language restrictions were not considered in searching the databases. Cochrane Library, Google Scholar, PubMed, EMBASE, ProQuest, CINAHL, Science Direct, MEDLINE, and Scopus were used to obtain the data from them. All data were scanned from the year 2000 until 2019. Papers selected for retrieval were evaluated by the Newcastle-Ottawa and CONSORT scales. Results: A total of 14 articles that met the inclusion criteria were ultimately assessed. Motile sperm, sperm vitality and membrane integrity, morphology, volume, total sperm count, sperm concentration, and sperm fertility were found to be influenced by radiofrequency waves. Conclusions: The results showed that RF has detrimental effects on semen parameters and due to an increase in RF wave use currently and its role in male infertility, giving information to men about adverse complications of RF is necessary. Further studies are needed to design the less harmful devices.

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Effects of Sperm Parameters on Pregnancy Rate in Patients Undergoing Intrauterine Insemination

Gynecology Obstetrics and Reproductive Medicine ◽

10.21613/gorm.2020.1040 ◽

2020 ◽

pp. 1

Author(s):

Mehmet Solakhan ◽

Mustafa Demir

Keyword(s):

Intrauterine Insemination ◽

Sperm Count ◽

Sperm Concentration ◽

Sperm Parameters ◽

Motile Sperm ◽

Progressive Motility ◽

Age Related ◽

Significant Difference ◽

Sperm Volume ◽

Infertile Couples

OBJECTIVE: In this study, the effects of sperm parameters on the success of intrauterine insemination were investigated. STUDY DESIGN: The data from 309 infertile couples who were admitted between 2012-2018 without a female factor were analyzed retrospectively and included in the study. After the administration of gonadotropin and hCG (5000-10000 IU), single insemination was performed in 36-40 hours in all cycles. All couples underwent routine infertility screening. The relationship between sperm parameters (motility, morphology, sperm count), patient age, duration of infertility with intrauterine insemination success was evaluated.RESULTS: There was no statistically significant difference between the two groups in terms of mean age and age related-parity. There was no statistically significant difference between male ages, liquefaction, and sperm volumes between the two groups (p=0.898, p=0.448, p=0.651). Before washing; There was a statistically significant difference between the sperm concentration, percentage of total motile sperm, percentage of progressive motility sperm, percentage of normal sperm morphology, and total sperm count between the two groups (p=0.0001, p=0.0001, p=0.0001, p=0.0001, p=0.0001). After sperm washing; the results were similar to those obtained before washing. While statistically significant difference was observed between sperm volume and sperm concentrations (p=0.023, p=0.018), no significant difference was observed between the two groups in total sperm count (p=0.612).CONCLUSION: As a result, during the application of intrauterine insemination to infertile couples, total motile sperm count, progressive motility sperm count ratio and high sperm ratio with normal morphology used in order to increase pregnancy success can be considered as criteria that increase the chances of success.

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Influence of Lifestyle and Environmental Factors on Semen Quality in Ghanaian Men

International Journal of Reproductive Medicine ◽

10.1155/2020/6908458 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Richard Michael Blay ◽

Abigail Duah Pinamang ◽

Augustine E. Sagoe ◽

Ewurama Dedea Ampadu Owusu ◽

Nii Koney-Kwaku Koney ◽

...

Keyword(s):

Environmental Factors ◽

Male Infertility ◽

Sperm Motility ◽

Semen Quality ◽

Sperm Quality ◽

Sperm Count ◽

Semen Analysis ◽

Reproductive Age ◽

Adult Males ◽

Lifestyle Habits

Introduction. Male infertility is known to contribute about half of all infertility cases. In Ghana, the prevalence of male infertility is higher (15.8%) than in females (11.8%). Sperm quality is associated with the likelihood of pregnancy and known to be the cause of male fertility problems 90% of the time. Exposure to certain environmental factors reduces semen quality in men. The study examined the effects of environmental and lifestyle factors on semen quality in Ghanaian men. Materials and Methods. This was a cross-sectional study involving 80 apparent healthy adult males in their reproductive age. Participants were males referred to the laboratory (Immunology Unit of the Korle-Bu Teaching Hospital) for semen analysis test and/or culture and sensitivity. Participants were made to fill out a questionnaire which entailed selected environmental factors (accidents or trauma, exposure to chemicals, radiation, and heat) and lifestyle habits (including alcohol consumption, smoking, and whether participants sat more or less than 4 hours per day). Semen samples were then collected by masturbation into sterile containers and analysed in accordance with WHO guidance for semen analysis within 60 minutes after ejaculation and collection. Results. About 69% of participants had semen pH within the normal range compared to 15% whose pH were lower than 7.2. There was a significantly high number of immotile sperm cells ( p value = 0.017) in participants who sat for more than 4 hours as compared to those that sat for less than 4 hours in a day. Active sperm motility and viability showed significant increase ( p value = 0.002 and 0.009, respectively) in participants who kept their cell phones in their side pockets. Smoking produced a twofold decrease in sperm count as smokers had a significantly lower sperm count ( 12.28 ± 10.95 × 10 6 /ml) compared to the smoke-free ( 23.85 ± 22.14 × 10 6 /ml). For exposure to STDs, no significant differences were recorded among study groups concerning semen quality. Conclusion. Sperm quality in Ghanaian men is associated with lifestyle habits. Smoking and sitting for long hours influenced sperm motility and count, respectively. Knowledge of the factors that influence sperm quality in this geographical region can contribute to informed decisions on effective management of infertility in Ghanaian men.

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Varicocele treatment: A 2-centers comparison between non microsurgical open correction, laparoscopic approach and retrograde percutaneous sclerotization on 463 cases

Archivio Italiano di Urologia e Andrologia ◽

10.4081/aiua.2013.3.143 ◽

2013 ◽

Vol 85 (3) ◽

pp. 143 ◽

Cited By ~ 1

Author(s):

Giangiacomo Ollandini ◽

Giovanni Liguori ◽

Stanislav Ziaran ◽

Tomáš Málek ◽

Giorgio Mazzon ◽

...

Keyword(s):

Sperm Count ◽

Laparoscopic Approach ◽

Sperm Parameters ◽

Age Related ◽

Number Of Patients ◽

Significant Difference ◽

Bivariate Regression ◽

Open Surgical Procedure ◽

Bivariate Regression Analysis

Objectives: To determine whether there are differences in sperm parameters improvement after different varicocele correction techniques. To determine the role of age in sperm parameters improvement. Methods: 2 different European centers collected pre- and postoperative sperm parameters of patients undergoing varicocele correction. Among 463 evaluated patients, 367 were included. Patients were divided in procedure-related and age-related groups. Ivanissevich inguinal open surgical procedure (OS), lymphatic-sparing laparoscopic approach (LSL) and retrograde percutaneous transfemoral sclerotization (RPS) were performed. As outcome measurements sperm count (millions/mL, SC) and percentage of mobile sperms were analyzed. Univariate and multivariate regression between the defined groups; bivariate regression analysis between age and sperm count and motility. Results: Number of patients: OS 78; LSL 85; RPS 204. Mean age 30.2 (SD 6.83); postoperative SC increased from 18.2 to 30.1 (CI 95% 27.3-32.9; p < 0,001); motility from 25.6 to 32.56% (30.9-34.2; p < 0.001). OS: SC varied from 16.9 to 18.2 (p < 0.001); sperm motility from 29% to 33% (p < 0.001). LSL: SC from 15.5 to 17.2 (p < 0.001); motility from 27 to 31% (p < 0.001). RPS: SC from 18.9 to 36.2 (p < 0.001); motility from 24% to 32% (p < 0.001). Univariate and multivariate analysis confirmed the significant difference of SC variation in RPS, compared to the other groups (p < 0.001). No significance between LSL and OS (p = 0.826). No significant differences regarding motility (p = 0.8). Conclusions: Varicocele correction is confirmed useful in improving sperm parameters; sclerotization technique leads to a better sperm improvement compared to other studied procedures; improvement in seminal parameters is not affected by age of the patients treated.

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Human Papillomavirus and Infertility

Medicina ◽

10.3390/medicina55070377 ◽

2019 ◽

Vol 55 (7) ◽

pp. 377 ◽

Cited By ~ 9

Author(s):

Jeršovienė ◽

Gudlevičienė ◽

Rimienė ◽

Butkauskas

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Sperm Quality ◽

Hpv Infection ◽

University Hospital ◽

Sperm Parameters ◽

World Health ◽

Abnormal Sperm ◽

Significant Difference ◽

High Risk Hpv

Background and objectives. Human papillomavirus (HPV) is the most commonly sexually transmitted infection. Recent evidence suggests that an HPV infection may affect fertility. The aim of the study was to determine the prevalence of HPV infections among couples undergoing in vitro fertilization (IVF) and to identify their awareness of HPV. Material and Methods. A total of 200 samples were collected from couples who received IVF treatment during 2017–2018 in Vilnius University Hospital Santaros Klinikos (VUH SK) Santaros Fertility Centre (SFC). For HPV detection, cervical swabs from women and sperm samples from men were taken and a real time polymerase chain reaction (RT-PCR) was used for the identification of 14 high-risk HPV types. Sperm parameters were evaluated according to World Health Organization (WHO) recommendations for 2010. Research subjects answered an anonymous questionnaire to ascertain their knowledge of HPV. Results. After testing of HPV in couples undergoing IVF, it was found that 33 out of 100 couples (33%) were HPV positive. Of these, 19% of women (19/100) and 20% of men (20/100) tested positive. Using Fisher’s exact test, a statistically significant difference was found between HPV infections and abnormal sperm quality parameters (p = 0.023). Conclusions. HPV may have an impact in spermatogenesis, because an HPV infection was more frequently detected in men with abnormal sperm parameters. High-risk HPV 52 was the most common genotype among couples undergoing IVF treatment.

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