scholarly journals Multi-omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer and product quality

2021 ◽  
Author(s):  
Alison Lee ◽  
Yee Jiun Kok ◽  
Meiyappan Lakshmanan ◽  
Dawn Leong ◽  
Lu Zheng ◽  
...  
Keyword(s):  
Product Quality ◽  
Specific Productivity ◽  
Cho Cell ◽  
Systematic Analysis ◽  
Product Titer ◽  
Culture Ph ◽  
Optimization And Control ◽  
Ph Conditions ◽  
And Control ◽  
Mab Production

A robust monoclonal antibody (mAb) bioprocess requires physiological parameters such as temperature, pH, or dissolved oxygen (DO) to be well-controlled as even small variations in them could potentially impact the final product quality. For instance, pH substantially affects N-glycosylation, protein aggregation and charge variant profiles, as well as mAb productivity. However, relatively less is known about how pH jointly influences product quality and titer. In this study, we investigated the effect of pH on culture performance, product titer and quality profiles by applying longitudinal multi-omics profiling, including transcriptomics, proteomics, metabolomics and glycomics, at three different culture pH set points. The subsequent systematic analysis of multi-omics data showed that pH set points differentially regulated various intracellular pathways including intracellular vesicular trafficking, cell cycle, and apoptosis, thereby resulting in differences in specific productivity, product titer and quality profiles. In addition, a time-dependent variation in mAb N-glycosylation profiles, independent of pH was identified to be mainly due to the accumulation of mAb proteins in the endoplasmic reticulum (ER) over culture time, disrupting cellular homeostasis. Overall, this multi-omics-based study provides an in-depth understanding of the intracellular processes in mAb-producing CHO cell line under varied pH conditions and could serve as a baseline for enabling the quality optimization and control of mAb production.

Online/at‐line measurement, analysis and control of product titer and critical product quality attributes (CQAs) during process development

2020 ◽  
Vol 117 (12) ◽  
pp. 3757-3765 ◽  
Author(s):  
Letha Chemmalil ◽  
Tanushree Prabhakar ◽  
June Kuang ◽  
Jay West ◽  
Zhijun Tan ◽  
...  
Keyword(s):  
Product Quality ◽  
Process Development ◽  
Quality Attributes ◽  
Product Titer ◽  
Measurement Analysis ◽  
Line Measurement ◽  
And Control

Management Optimization and Control in Manufacturing Process Based on Knowledge-Flow

2011 ◽  
Vol 308-310 ◽  
pp. 1361-1364
Author(s):  
Gui Qin Li ◽  
Peter Mitrouchev ◽  
Song Lin ◽  
Li Xin Lu ◽  
Daniel Brissaud
Keyword(s):  
Product Quality ◽  
Control Process ◽  
Social Background ◽  
Knowledge Flow ◽  
Analysis Software ◽  
The Social ◽  
Optimization And Control ◽  
Management Optimization ◽  
The One ◽  
And Control

Knowledge management plays an important role in the management and control of product quality. In order to improve the efficiency of knowledge utilization in the product processing and study the knowledge flow with consideration of knowledge providers, a new method based on social network analysis is proposed in this work. Considering the social background of the different knowledge providers, the center degree of each provider could be obtained. With this value of center degree, the position of different kinds of knowledge could be calculated as well. Meanwhile, the use of correlation analysis software could help to optimize the tactic of task. Thus, on the one hand, the using and the flow efficiency of the knowledge in the product processing can be optimized more comprehensively, and on the other hand the management and control process of the product quality could be greatly improved.

Studi Kualitas Puding Melalui Pendekatan Six Sigma Studi Kasus Di Pt. Keong Nusantara Abadi

2019 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Rony Trizudha ◽  
Sri Rahayuningsih ◽  
Ana Komari
Keyword(s):  
Quality Control ◽  
Quality Improvement ◽  
Production Process ◽  
Product Quality ◽  
Six Sigma ◽  
Improve Quality ◽  
High Productivity ◽  
Fierce Competition ◽  
And Control ◽  
Technology Advances

As technology advances at this time, players in business are aware of the importance of product quality in the increasingly fierce competition in the industrial world due to the emergence of many similar companies. Therefore, companies must be able to compete to meet customer desires and try to retain customers. To maintain customers and their marketing areas, companies must have high competitiveness in order to survive by prioritizing quality improvement, increasing efficiency and increasing productivity to improve quality because by increasing quality, products can be accepted among consumers so that company goals can be fulfilled. Therefore, the company must carry out effective quality control which will result in high productivity, lower overall cost of making goods and the factors that cause production failure to be minimized. To improve quality, use the six sigma method, DMAIC and seven tools so that it can be known the cause of the damage and what actions are taken so that there needs to be a controversy to stabilize the processes of the production process so that we can know what percentage of damage and what factors cause damage, therefore there must be measurements and recommendations for improvement and control to reduce the causes From the analysis, it was found that the dent cup was 20.36%, the lid was 21.36% less dense, the lid was damaged in the finished product 18.72%, the cup was 19.28% less thick, the packaging was flexible 20.55%Seiring kemajuan teknologi pada saat ini pelaku di bisnis menyadari akan pentingnya kualitas produk dalam persaingan dunia industri yang semakin ketat karena banyak bermunculan perusahaan-perusahaan sejenis. Oleh sebab itu perusahaan harus dapat bersaing untuk memenuhi keinginan  pelanggan dan berusaha dapat mempertahankan pelanggan. Untuk mempertahankan pelangan dan wilayah pemasaranya perusahaan-perusahaan harus mempunyai daya saing yang tinggi untuk dapat bertahan dengan mengutamakan peningkatan mutu, peningkatan efisiensi dan peningkatan produktivitas untuk meningkatkan kualitas karena dengan peningkatan kualitas, produk dapat diterima di kalangan konsumen sehingga tujuan perusahaan dapat terpenuhi. Maka dari itu perusahaan harus melakukan pengendalian kualitas yang efektif akan menghasilkan produktivitas yang tinggi, biaya pembuatan barang keseluruhan yang lebih  rendah serta  faktor-faktor yang menyebabkan kegagalan produksi akan dapat ditekan sekecil mungkin. Untuk meningkatkan kualitas mengunakan metode six sigma, DMAIC dan seven tools agar dapat diketahui penyebab  kerusakan  dan  tindakan  apa  saja  yang dilakukan sehingga perlu ada kontror untuk menstabilkan  peoses proses produksi sehinga dapat di ketahui berapa persen  kerusakan dan faktor-faktor apa saja yang menyebabkan  kerusakan maka dari itu harus ada pengukuran dan  rekomendasi perbaikan serta melakukan kontrol untuk mengurangi penyebab kerusakan. Dari hasil analisis  di ketahui cup  penyok 20,36%, lid kurang  rapat  21,36%, lid  rusak  pada produk jadi 18,72%,cup kurang tebal 19,28 %kemasan lentur 20,55%

Five-Lump Kinetic Model for the Catalytic Cracking Process\

2018 ◽  
Vol 69 (10) ◽  
pp. 2633-2637
Author(s):  
Raluca Dragomir ◽  
Paul Rosca ◽  
Cristina Popa
Keyword(s):  
Kinetic Model ◽  
Programming Language ◽  
Catalytic Cracking ◽  
Computational Method ◽  
Industrial Data ◽  
New Model ◽  
Optimization And Control ◽  
Cracking Process ◽  
And Control ◽  
Matlab Programming

The main objectives of the present paper are to adaptation the five-kinetic model of the catalytic cracking process and simulation the riser to predicts the FCC products yields when one of the major input variable of the process is change. The simulation and adaptation are based on the industrial data from Romanian refinery. The adaptation is realize using a computational method from Optimization Toolbox from Matlab programming language. The new model can be used for optimization and control of FCC riser.

Optimization and Control Petroleum Refinery Wastewater Treatment Systems — Status, Trends and Needs

1989 ◽  
Vol 24 (3) ◽  
pp. 463-477
Author(s):  
Stephen G. Nutt
Keyword(s):  
Wastewater Treatment ◽  
Process Control ◽  
Petroleum Refinery ◽  
Control Strategies ◽  
Refinery Wastewater ◽  
Statistical Process ◽  
Petroleum Refinery Wastewater ◽  
Optimization And Control ◽  
And Control ◽  
Wastewater Treatment Systems

Abstract Based on discussions in workshop sessions, several recurring themes became evident with respect to the optimization and control of petroleum refinery wastewater treatment systems to achieve effective removal of toxic contaminants. It was apparent that statistical process control (SPC) techniques are finding more widespread use and have been found to be effective. However, the implementation of real-time process control strategies in petroleum refinery wastewater treatment systems is in its infancy. Considerable effort will need to be expended to demonstrate the practicality of on-line sensors, and the utility of automated process control in petroleum refinery wastewater treatment systems. This paper provides a summary of the discussions held at the workshop.

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