scholarly journals Genome Editing accelerated Re-Domestication” (GEaReD) – a new major direction in plant breeding

Author(s):  
Tobias Hanak ◽  
Claus Madsen ◽  
Henrik Brinch-Pedersen

The effects of climate change, soil depletion, a growing world population putting pressure on food safety and security are major challenges for agriculture in the 21st century. The breeding success of the green revolution has decelerated and current programs can only offset the yield affecting factors. New approaches are urgently needed and we propose, “Genome Editing accelerated Re-Domestication” (GEaReD) as a major new direction in plant breeding. By combining the upcoming technologies for phenotyping, omics and artificial intelligence with the promising new CRISPR-toolkits, this approach is closer than ever. Wild relatives of current crops are often adapted to harsh environments and have a high genetic diversity. Re-domestication of wild barley or teosinte could generate new cultivars adapted to environmental changes. De novo domestication of perennial relatives like Hordeum bulbosum could counter with soil depletion and increase soil carbon. Recent research already proved the principle of re-domestication in tomato and rice and therefore laid the foundation for GEaReD.

2021 ◽  
pp. 251484862110224
Author(s):  
Leila Rezvani

Using Donna Haraway’s notion of “response-ability”, or the cultivation of the capacity for response, this paper seeks to understand seed saving and plant breeding as politically and ethically charged modes of interspecies communication. In Brittany, France, a region known for its industrial-scale fresh vegetable production, peasant farmers and organic plant breeders question the modernist plant breeding and agro-industrial paradigm, cross-pollinating ideas to produce new understandings of genotype-environment interaction, biodiversity and heredity. Plant liveliness is understood as politically transformative, constitutive of an agriculture that supports peasant farmer and crop plant creativity and self-determination. In contrast to F1 hybrids, open-pollinated semences paysannes (peasant seed) retain the ability to respond to environmental changes, adapt and evolve over (human and plant) generations. Farmers must in turn engage specific modes of attention, interpreting plant expressions and shaping future generations through rouging and crossing, selecting and saving, watching and learning from their crops. Mutual response is the foundation of interdependence, in which nonconspecific partners adjust to one another’s ways of being and doing in order to labor together. In remaining response-able, farmers reckon with the liveliness and agential capacities of plants, qualities that work against their subsumption into factory-like methods of cultivation. These communicative practices hint at the radical potential for interspecies resistance to monoculture within plant breeding and cultivation, practices that are so often molded by the interests of agro-industrial capital.


2021 ◽  
Vol 22 (11) ◽  
pp. 5585
Author(s):  
Sajid Fiaz ◽  
Sunny Ahmar ◽  
Sajjad Saeed ◽  
Aamir Riaz ◽  
Freddy Mora-Poblete ◽  
...  

A world with zero hunger is possible only through a sustainable increase in food production and distribution and the elimination of poverty. Scientific, logistical, and humanitarian approaches must be employed simultaneously to ensure food security, starting with farmers and breeders and extending to policy makers and governments. The current agricultural production system is facing the challenge of sustainably increasing grain quality and yield and enhancing resistance to biotic and abiotic stress under the intensifying pressure of climate change. Under present circumstances, conventional breeding techniques are not sufficient. Innovation in plant breeding is critical in managing agricultural challenges and achieving sustainable crop production. Novel plant breeding techniques, involving a series of developments from genome editing techniques to speed breeding and the integration of omics technology, offer relevant, versatile, cost-effective, and less time-consuming ways of achieving precision in plant breeding. Opportunities to edit agriculturally significant genes now exist as a result of new genome editing techniques. These range from random (physical and chemical mutagens) to non-random meganucleases (MegaN), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein system 9 (CRISPR/Cas9), the CRISPR system from Prevotella and Francisella1 (Cpf1), base editing (BE), and prime editing (PE). Genome editing techniques that promote crop improvement through hybrid seed production, induced apomixis, and resistance to biotic and abiotic stress are prioritized when selecting for genetic gain in a restricted timeframe. The novel CRISPR-associated protein system 9 variants, namely BE and PE, can generate transgene-free plants with more frequency and are therefore being used for knocking out of genes of interest. We provide a comprehensive review of the evolution of genome editing technologies, especially the application of the third-generation genome editing technologies to achieve various plant breeding objectives within the regulatory regimes adopted by various countries. Future development and the optimization of forward and reverse genetics to achieve food security are evaluated.


Plant Science ◽  
2017 ◽  
Vol 256 ◽  
pp. 120-130 ◽  
Author(s):  
Agustin Zsögön ◽  
Tomas Cermak ◽  
Dan Voytas ◽  
Lázaro Eustáquio Pereira Peres

Author(s):  
Y Widodo ◽  
S Wahyuningsih ◽  
JS Utomo ◽  
A Subagio

Green revolution started at mid of twentieth century was the answer of anxiousness reminded by Malthusian that food scarcity problems in relation with population growth. In concurrence with exploitation of fossil fuel for agriculture mechanization as well as agrochemicals in the form of inorganic fertilizer and pesticide, green revolution by introducing high yielding varieties of cereals and grains was able to nourish the world population by increasing productivity. Indeed, from beginning of mechanization with fossil fuel based as advised by Rudolf Diesel then Arrhenius would be affected to the release of CO2 to the atmosphere and consequently exaggerating climate change as suffered by current and future generations. Under green revolution based on cereals and grains affected forest conversion into open agricultural land, because both commodities are sun-loving crops, which are hate to the shade. On the other hand, to slow the severity of climate change natural forest must be conserved tightly. Entering third millennium demand of food production with ecologically friendly is stronger. Hence, green revolution needs to be amended into greener perspectives. Thus, implementation of agro-forestry into wide range of agro-ecological zone is urgently innovated. Fortunately, shade tolerant of root crops has significant advantage to be developed under agro-forestry. Under shade of forest canopy at basal forest strata, root crops are able to sequester CO2 to be converted into carbohydrate and other compounds to provide food for the dweller. Back to nature is not only a slogan, with root crops under agro-forestry is a reality; fresh root up to 30 t ha-1 can be harvested yearly as the source of food and renewable fuel as well. This potential is very worthy to improve and greening the existing green revolution to be more sustainable.Int. J. Agril. Res. Innov. & Tech. 8 (1): 26-37, June, 2018


2006 ◽  
Vol 4 (1) ◽  
pp. 54-63 ◽  
Author(s):  
Niels P. Louwaars ◽  
Eva Thörn ◽  
José Esquinas-Alcázar ◽  
Shumin Wang ◽  
Abebe Demissie ◽  
...  

Applied genetics combined with practical plant breeding is a powerful tool in agricultural development and for food security. The Green Revolution spurred the world's potential to meet its food, feed and fibre needs at a time when vast regions were notoriously food-insecure. Subsequent adaptations of such strategies, from the late 1980s onwards, in order to develop new plant varieties in a more participatory way, have strengthened the focus on applying technology to farmers' diverse needs, feeding research results into a variety of seed systems. During these developments, there were no major legal impediments to the acquisition of either local or formal knowledge or of the building blocks of plant breeding: genetic resources. The emergence of molecular biology in plant science is creating a wealth of opportunities, both to understand better the limitations of crop production and to use a much wider array of genetic diversity in crop improvement. This ‘Gene Revolution’ needs to incorporate the lessons from the Green Revolution in order to reach its target groups. However, the policy environment has changed. Access to technologies is complicated by the spread of private rights (intellectual property rights), and access to genetic resources by new national access laws. Policies on access to genetic resources have changed from the concept of the ‘Heritage of Mankind’ for use for the benefit of all mankind to ‘National Sovereignty’, based on the Convention on Biological Diversity, for negotiated benefit-sharing between a provider and a user. The Generation Challenge Programme intends to use genomic techniques to identify and use characteristics that are of value to the resource-poor, and is looking for ways to promote freedom-to-operate for plant breeding technologies and materials. Biodiversity provides the basis for the effective use of these genomic techniques. National access regulations usually apply to all biodiversity indiscriminately and may cause obstacles or delays in the use of genetic resources in agriculture. Different policies are being developed in different regions. Some emphasize benefit-sharing, and limit access in order to implement this (the ‘African Model Law’), while others, in recognition of countries' interdependence, provide for facilitated access to all genetic resources under the jurisdiction of countries in the region (the Nordic Region). There are good reasons why the use of agricultural biodiversity needs to be regulated differently from industrial uses of biodiversity. The International Treaty on Plant Genetic Resources for Food and Agriculture, which entered into force in 2004, provides for facilitated access to agricultural genetic resources, at least for the crops that are included in the Treaty's ‘Multilateral System of Access and Benefit-sharing’. Ratification of the Treaty is proceeding apace, and negotiations have entered a critical stage in the development of practical instruments for its implementation. Although the scope of the Treaty is all plant genetic resources for food and agriculture, there are important crops that are not covered by its Multilateral System. Humanitarian licences are being used to provide access for the poor to protected technologies: countries may need to create such a general humanitarian access regime, to ensure the poor have the access they need to agricultural genetic resources.


2018 ◽  
pp. 605-644
Author(s):  
D. R. Mehta ◽  
A. K. Nandha

2020 ◽  
Vol 9 (4) ◽  
pp. 318-325 ◽  
Author(s):  
Anna Olsson-Brown ◽  
Rosemary Lord ◽  
Joseph Sacco ◽  
Jonathan Wagg ◽  
Mark Coles ◽  
...  

Introduction Immune checkpoint inhibitors can lead to thyroid dysfunction. However, the understanding of the clinical phenotype of ICI-induced thyroid dysfunction in the real-world population is limited. The purpose of this study was to characterise the clinical patterns of dysfunction and evaluate the demographic, biochemical and immunological features associated with this patient cohort. Materials and methods To characterise the longitudinal clinical course of thyroid dysfunction in patients from a single, UK regional cancer centre, a retrospective review of patients was conducted. Inclusion criteria included all patients treated with antiPD-1 checkpoint inhibitors (ICI), either as monotherapy (pembrolizumab/nivolumab) or in combination with a CTLA-4 inhibitor (ipilimumab). Patterns of toxicity were evaluated together with assessment of antibody titres. Results Over 16 months, thyroid dysfunction was seen in 13/90 and 3/13 patients treated with anti-PD1 monotherapy and in combination with ipilimumab, respectively. Patients either developed hyperthyroidism followed by hypothyroidism (12/16) or de novo hypothyroidism (4/16). Most patients were female (n = 11). All patients required thyroid replacement therapy. There was no relationship between clinical pattern of dysfunction and the presence of thyroid autoantibodies. Conclusions There are two distinct patterns of thyroid dysfunction in ICI-treated patients. Patients with thyroiditis develop subsequent hypothyroidism in the vast majority of cases. The potential benefit from steroids or other therapy to manage the hyperthyroid phase remains unclear. Early detection of these patients through appropriate monitoring will improve clinical management and early hormone replacement, reducing the symptomatic burden of hypothyroidism.


2021 ◽  
Author(s):  
Venera S. Kamburova ◽  
Ilkhom B. Salakhutdinov ◽  
Shukhrat E. Shermatov ◽  
Ibrokhim Y. Abdurakhmonov

The main task of plant breeding is creating of high-yield, resistant to biotic and abiotic stresses crop varieties with high product quality. The using of traditional breeding methods is limited by the duration of the new crop varieties creation with the required agronomic traits. This depends not only on the duration of growing season and reaching of mature stage of plants (especially the long-period growth plants, e.g. trees), as well as is associated with applying of multiple stages of crossing, selection and testing in breeding process. In addition, conventional methods of chemical and physical mutagenesis do not allow targeting effect to genome. However, the introduction of modern DNA-technology methods, such as genome editing, has opened in a new era in plant breeding. These methods allow to carry out precise and efficient targeted genome modifications, significantly reducing the time required to get plants with desirable features to create new crop varieties in perspective. This review provides the knowledge about application of genome editing methods to increase crop yields and product quality, as well as crop resistance to biotic and abiotic stresses. In addition, future prospects for integrating these technologies into crop breeding strategies are also discussed.


2005 ◽  
Vol 20 (1) ◽  
pp. 5-14 ◽  
Author(s):  
L.R. DeHaan ◽  
D.L. Van Tassel ◽  
T.S. Cox

AbstractPerennial grain crops would address many agricultural problems, including soil erosion, nutrient loss and pesticide contamination. Doubts about the possibility of perennial grain crops rest upon two assumptions: (1) that the relationship between yield and longevity is a fixed function that cannot be influenced by selection, mutation or environmental changes; and (2) that yield and longevity trade off in a bivariate manner to the exclusion of all other traits. These assumptions are consistent with the phenotypic trade-off model, but recent research suggests that a quantitative genetic model is a more appropriate approach to trade-offs. In the quantitative genetic model, environmental and genetic changes can result in increases in two traits simultaneously even when a trade-off, or negative correlation, exists between the two traits. Empirical evidence that the trade-off between perenniality and reproductive allocation is not fixed comes from wild, herbaceous perennials that can produce more than 2000 kg seed ha−1 in the temperate zone, and herbaceous perennial crops that produce on average 8900 kg fruit ha−1 in the tropics. Ecological literature suggests that most perennials produce small amounts of seed relative to their vegetative growth not as a physiological absolute, but rather as a result of natural selection in a stable, competitive environment favoring longevity. By selecting strongly for seed yield in a population of perennial plants, the plant breeder can likely achieve that which is rare in nature—a high seed-yielding perennial plant. The same general methodologies that have allowed annual grain breeders to increase grain yield and push many combinations of negatively correlated traits to levels of expression not seen in nature are available to the perennial grain breeder. Perennial grain breeders are integrating ecological principles and traditional plant breeding methods in their efforts to develop perennial grain wheat (Triticum spp.), sorghum (Sorghum spp.), sunflower (Helianthus spp.), Illinois bundleflower (Desmanthus illinoensis) and rice (Oryza spp.).


Traditional plant breeding depends on spontaneous and induced mutations available in the crop plants. Such mutations are rare and occur randomly. By contrast, molecular breeding and genome editing are advanced breeding techniques that can enhance the selection process and produce precisely targeted modifications in any crop. Identification of molecular markers, based on SSRs and SNPs, and the availability of high-throughput (HTP) genotyping platforms have accelerated the process of generating dense genetic linkage maps and thereby enhanced application of marker-assisted breeding for crop improvement. Advanced molecular biology techniques that facilitate precise, efficient, and targeted modifications at genomic loci are termed as “genome editing.” The genome editing tools include “zinc-finger nucleases (ZNFs),” “transcription activator-like effector nucleases (TALENs),” oligonucleotide-directed mutagenesis (ODM), and “clustered regularly interspersed short palindromic repeats (CRISPER/Cas) system,” which can be used for targeted gene editing. Concepts of molecular plant breeding and genome editing systems are presented in this chapter.


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