scholarly journals Computational modelling to predict mechanosensing of fibroblast cells in response to different material properties

2021 ◽  
Author(s):  
Wenjian Yang ◽  
Ma Luo ◽  
Yanfei Gao ◽  
Jinju Chen
Keyword(s):  
Dynamic Change ◽  
Healing Process ◽  
Computational Modelling ◽  
Cell Communication ◽  
Focal Adhesions ◽  
Fibroblast Cell ◽  
Wound Healing Process ◽  
Depth Sensing ◽  
Stress Fibre ◽  
Cell Cell

We present a computational model of the mechanosensing of a fibroblast cell seeded on the materials with different stiffnesses and thicknesses. The model can predict the critical thickness of a given biomaterial that a cell can sense and the dynamic change of stress fibres and focal adhesions through its incorporation of the dynamic characteristics of stress fibre contraction and focal adhesion. We show that the cell-cell communication via elastic substrate induces the orientation of stress fibres. The cell-cell interaction through compliant substrate has a small but significant effect on enhancing the cell depth sensing capability in terms of interfacial displacement and stress fibre concentration. The framework developed here is important for a thorough understanding of processes where substrates are deformed such as in wound healing process and the design of bioactive coatings for tissue engineering.

Serum cytokine profile during subcutaneous implantation of the decellularized esophagus matrix in rats

2020 ◽  
Vol 35 (3) ◽  
pp. 446-455
Author(s):  
Karina Melkonyan ◽  
Ramazan Nakokhov ◽  
Tatyana Rusinova ◽  
Yana Yutskevich ◽  
Ilya Bykov ◽  
...  
Keyword(s):  
Dynamic Change ◽  
Healing Process ◽  
Cytokine Profile ◽  
Control Group ◽  
Wound Healing Process ◽  
Subcutaneous Implantation ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Rat Serum ◽  
Gm Csf

Aim to study non-specific immune response characteristics (serum cytokine profile) in rats after subcutaneous implantation of the decellularized esophagus matrix. Methods Data were obtained in Wistar rats. The rats underwent subcutaneous implantation of decellularized esophagus (DE) and native allogeneic esophagus (NE). Explantation of sampling were carried out on the 7th, 14th and 21st day of the experiment. Explanted NEs and DEs were processed for histologic examination. The content of IL1α, IL2, IL4, IL17А, TNFα, IFNγ and GM-CSF in serum samples were tested by ELISA. Results In rat serum with DEs on the 7th day of the experiment it was significant increase in IL1α level in comparison with control group, IL2, TNFα, IL4 levels did not differ from the control group levels that indicates the stabilization of inflammation. The content of IL17A, IFNγ and GM-CSF significantly decreased compared to control. On the 14th day, IL17A concentration analysis showed a sharp decrease in comparison with the the 7th experimental day. We found decrease in IL1α level vs control group and decrease in IFNγ level vs 7th day. On the 21st experimental day was shown a significant decrease in the IL17A, IFNγ and IL1α content in DE rats. Conclusions It was found dynamic change in studied rat cytokine concentrations that correspond to favourable clinical picture in DE group in comparison with an active inflammatory reaction in NE group. IL1α, IL4, IL17A and IFNγ concentrations reflect positive dynamics of the wound healing process and the absence of local inflammation and rejection of decellularized matrices.

scholarly journals Effect of Polysaccharides from Bletilla striata on the Healing of Dermal Wounds in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Chen Zhang ◽  
Yanan He ◽  
Zhejie Chen ◽  
Jinfeng Shi ◽  
Yan Qu ◽  
...  
Keyword(s):  
Health Care ◽  
Wound Healing ◽  
Healing Process ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Wound Healing Process ◽  
Skin Defect ◽  
Full Thickness ◽  
Bletilla Striata

Bletilla striata has been largely used in traditional folk medicine in China as a wound healing agent and to treat gastritis and several other health problems. Some studies have shown that plant polysaccharides may have the ability to promote wound healing. The aim of this work was to evaluate the wound healing activity of the polysaccharide extracted from Bletilla striata. Firstly, a Bletilla striata polysaccharide was extracted by water extraction and alcohol precipitation and characterized by Fourier transform infrared spectroscopy. The Bletilla striata polysaccharide was then tested for cell migration and proliferation using the mouse fibroblast cell line. Then, the Bletilla striata hydrogel was fabricated for acute wound health care of the mouse full-thickness excision. The results showed that the BSP enhanced the proliferation and migration of L929 cells. The superior wound healing capacity of the BSP hydrogel was demonstrated that it significantly accelerated the wound healing process in vivo in full-thickness skin defect wounded models. Compared to the saline group, the BSP hydrogel could accelerate wound healing and promote re-epithelialization and collagen deposition by means of TGF-β/Smad signal pathway activation. Taken together, BSP hydrogel would be a useful pharmaceutic candidate for acute cutaneous wound health care.

scholarly journals A central role for vimentin in regulating repair function during healing of the lens epithelium

2014 ◽  
Vol 25 (6) ◽  
pp. 776-790 ◽  
Author(s):  
A. S. Menko ◽  
B. M. Bleaken ◽  
A. A. Libowitz ◽  
L. Zhang ◽  
M. A. Stepp ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Wound Closure ◽  
Cell Function ◽  
Healing Process ◽  
Critical Role ◽  
Focal Adhesions ◽  
Lens Epithelium ◽  
Wound Healing Process ◽  
Vimentin Filaments

Mock cataract surgery provides a unique ex vivo model for studying wound repair in a clinically relevant setting. Here wound healing involves a classical collective migration of the lens epithelium, directed at the leading edge by an innate mesenchymal subpopulation of vimentin-rich repair cells. We report that vimentin is essential to the function of repair cells as the directors of the wound-healing process. Vimentin and not actin filaments are the predominant cytoskeletal elements in the lamellipodial extensions of the repair cells at the wound edge. These vimentin filaments link to paxillin-containing focal adhesions at the lamellipodial tips. Microtubules are involved in the extension of vimentin filaments in repair cells, the elaboration of vimentin-rich protrusions, and wound closure. The requirement for vimentin in repair cell function is revealed by both small interfering RNA vimentin knockdown and exposure to the vimentin-targeted drug withaferin A. Perturbation of vimentin impairs repair cell function and wound closure. Coimmunoprecipitation analysis reveals for the first time that myosin IIB is associated with vimentin, linking vimentin function in cell migration to myosin II motor proteins. These studies reveal a critical role for vimentin in repair cell function in regulating the collective movement of the epithelium in response to wounding.

Efficiency Improvement of Gelatin-Based Wound Dressings by Silk Fibroin and Chitosan

2020 ◽  
Vol 995 ◽  
pp. 97-102
Author(s):  
Wassanai Wattanutchariya ◽  
Kittiya Thunsiri ◽  
Suwichchaya Puntawang
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Silk Fibroin ◽  
Healing Process ◽  
Fibrous Tissue ◽  
Fibroblast Cell ◽  
The Body ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Moisture Condition

Wound healing is a natural process of human body. When the wound size exceeds the critical point for naturally body healing, the fibrous tissue will play their parts and created a scar. Therefore, extra treatment has been added to eliminate the body limitations. Currently, there are a lot of commercial bioactive wound healing and dressing due to its physiological and biological abilities. In wound healing process, high moisture condition is also required. In order to accelerate the wound healing process, Tissue engineering (TE) is recommended. The increasing of cell proliferation by TE will be increased the chance for wound healing acceleration. In this study, the combination of Gelatin (Gel), Chitosan (CS) and Silk Fibroin (SF) were varied mixed in 10 ratios and fabricated the structure by lyophilisation technique. The elastic ability, biodegradability, structure and pore morphology, porosity, swelling ability, and biotoxicity were observed in each ratio. Gel provided highest elastic ability and biodegradability. The addition of SF and CS in Gel decreased biodegradation rate and activate fibroblast cell proliferation. Therefore, CS and SF could increase efficiency of gelatin-base wound dressings for a variety of utilization.

scholarly journals Metabolic Reprogramming of Fibroblasts as Therapeutic Target in Rheumatoid Arthritis and Cancer: Deciphering Key Mechanisms using Computational Systems Biology Approaches

2020 ◽  
Author(s):  
Sahar Aghakhani ◽  
Naouel Zerrouk ◽  
Anna Niarakis
Keyword(s):  
Rheumatoid Arthritis ◽  
Extracellular Matrix ◽  
Systems Biology ◽  
Healing Process ◽  
Computational Modelling ◽  
Metabolic Reprogramming ◽  
Critical Event ◽  
Wound Healing Process ◽  
Computational Systems Biology ◽  
Structural Framework

Fibroblasts, the most abundant cells in the connective tissue, are key modulators of the extracellular matrix (ECM) composition. These spindle-shaped cells are capable of synthesizing various extracellular matrix proteins and collagen. They also provide the structural framework (stroma) for tissues and play a pivotal role in the wound healing process. While they are maintainers of the ECM turnover and regulate several physiological processes, they can also undergo transformations responding to certain stimuli and display aggressive phenotypes that contribute to disease pathophysiology. In this review, we focus on the metabolic pathways of glucose and highlight metabolic reprogramming as a critical event that contributes to the transition of fibroblasts from quiescent to activated and aggressive cells. We also cover the emerging evidence that allows us to draw parallels between fibroblasts in autoimmune disorders and more specifically in rheumatoid arthritis and cancer. We link the metabolic changes of fibroblasts to the toxic environment created by the disease condition and discuss how targeting of metabolic reprogramming could be employed in the treatment of such diseases. Lastly, we discuss Systems Biology approaches, and more specifically, computational modelling, as a means to elucidate pathogenetic mechanisms and accelerate the identification of novel therapeutic targets.

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