scholarly journals Evaluation of culturable, nitrifying, ammonium-oxidizing, and metabolically active bacteria in shrimp farm effluents of Kino Bay, Sonora, México

2012 ◽  
Vol 64 (3) ◽  
pp. 895-909 ◽  
Author(s):  
Nolberta Huerta-Aldaz ◽  
M.A. López-Torres ◽  
J.E. Valdez-Holguín ◽  
R.H. Barraza-Guardado
Keyword(s):  
Heterotrophic Bacteria ◽  
Natural Populations ◽  
Control Site ◽  
Epifluorescence Microscopy ◽  
Nitrifying Bacteria ◽  
Production Cycle ◽  
Mean Values ◽  
Discharge Area ◽  
Shrimp Farm ◽  
Active Bacteria

Bacteria from shrimp farm effluents in Kino Bay, Sonora, M?xico were evaluated during the 2008 production cycle. The culturable bacterial populations considered were viable heterotrophic bacteria (VHB) and Vibrio-like bacteria (VLB). In addition, total bacteria (TB), metabolically active bacteria (MAB), nitrifying bacteria (NB), and ammonium-oxidizing bacteria (AOB) were quantified by epifluorescence microscopy. Three sampling sites were established in the influence area of drain and one control site far from the discharge area. Average concentrations of VHB and VLB were 103 and 102 CFU mL-1, respectively. The TB ranged from 107 to 109 cells mL-1. The mean values of Mrelated to TB counts in the affected area were higher throughout the culture cycle compared to the control site, ranging from 1.09 to 27.35%. These results indicate that effluents modify the natural loads of bacteria in the discharge area, which could affect natural populations and the microbial balance of the area.

scholarly journals Effluents of Shrimp Farms and Its Influence on the Coastal Ecosystems of Bahía de Kino, Mexico

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ramón H. Barraza-Guardado ◽  
José A. Arreola-Lizárraga ◽  
Marco A. López-Torres ◽  
Ramón Casillas-Hernández ◽  
Anselmo Miranda-Baeza ◽  
...  
Keyword(s):  
Organic Matter ◽  
Suspended Solids ◽  
Heterotrophic Bacteria ◽  
Coastal Ecosystems ◽  
Control Site ◽  
Shrimp Culture ◽  
Shrimp Farm ◽  
Material Load ◽  
The Impact ◽  
Shrimp Farms

The impact on coastal ecosystems of suspended solids, organic matter, and bacteria in shrimp farm effluents is presented. Sites around Bahía de Kino were selected for comparative evaluation. Effluent entering Bahia Kino (1) enters Laguna La Cruz (2). A control site (3) was outside the influence of effluents. Water quality samples were collected every two weeks during the shrimp culture period. Our data show that the material load in shrimp farm effluents changes biogeochemical processes and aquatic health of the coastal ecosystem. Specifically, the suspended solids, particulate organic matter, chlorophylla, viable heterotrophic bacteria, andVibrio-like bacteria in the bay and lagoon were two- to three-fold higher than the control site. This can be mitigated by improvements in the management of aquaculture systems.

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

Distribution of nitrifying bacteria in a shallow stream

1997 ◽  
Vol 36 (8-9) ◽  
pp. 161-166 ◽  
Author(s):  
Ivana Jancarkova ◽  
Tove A. Larsen ◽  
Willi Gujer
Keyword(s):  
Field Experiments ◽  
Heterotrophic Bacteria ◽  
Pollution Source ◽  
Nitrifying Bacteria ◽  
Channel Water ◽  
River Bed ◽  
Hydraulic Conditions ◽  
Major Floods ◽  
Shallow Stream ◽  
Stream Bed

A project investigating the dynamics of self-purification processes in a shallow stream is carried out. Effects of the concentration gradient due to the distance to the pollution source, of hydraulic conditions in the river bed and of storm floods on the distribution of nitrifying bacteria were studied with the help of laboratory and field experiments. Nitrifiers density on the surface of the stream bed increased rapidly up to a distance of 300 m from the WWTP indicating possible competition of the nitrifiers with the heterotrophic bacteria close to the WWTP. Afterwards a slight decrease in the downstream direction was observed. In vertical profiles, higher bacterial densities were found at sites with rapid infiltration of channel water to the stream bed than at sites with no exchange between channel water and stream bed water or where stream bed water exfiltrated. A major flood event scoured the nitrifiers nearly totally from the surface of the river bed. Major floods belong so to the most dominant processes controlling self-purification in shallow streams. Minor floods, however, don't scour bacteria in the depth of the stream bed that could then be important for the self-purification processes.

scholarly journals Intestinal Microbial Ecology and Fillet Metal Chemistry of Wild Grey Mullets Reflect the Variability of the Aquatic Environment in a Western Mediterranean Coastal Lagoon (Santa Giusta, Sardinia, Italy)

Water ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 879
Author(s):  
Rosanna Floris ◽  
Gabriele Sanna ◽  
Cecilia Teodora Satta ◽  
Carlo Piga ◽  
Francesco Sanna ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Environmental Variability ◽  
Heterotrophic Bacteria ◽  
Active Role ◽  
Fish Muscle ◽  
Mean Value ◽  
Western Mediterranean ◽  
Mean Values ◽  
Abiotic And Biotic Factors ◽  
Grey Mullets

Fish populations play an active role in the maintenance of aquatic ecosystems biodiversity. Their intestinal microbiota and fillet chemistry depend on abiotic and biotic factors of the water environments that they inhabit. The present study investigated the grey mullets’ gut microbiota from a transitional aquatic ecosystem (Santa Giusta Lagoon, Sardinia, Italy) by a multidisciplinary approach which refers the results of (1) gut cultivable microbiota analyses (MA), (2) the trace metal assessment of fish muscle (TM), (3) the physico-chemical water monitoring (PC). MA detected the greatest number of total aerobic heterotrophic bacteria, Enterobacteriaceae and coliforms in Autumn (mean values 1.3 × 105, 2.4 × 104, 1.1 × 104 cfu g−1, respectively) when the accumulated rain and mean values of nutrients (reactive phosphorous and silica) were the highest. Marine bacteria were more numerous in Summer (mean value 7.4 × 105 cfu g−1) when the highest mean values of water temperature and salinity were registered. The gut bacteria were identified as Pseudomonas spp. (64%), Aeromonas spp. (17%), Ochrobactrum pseudogrignonense (10%), Providencia spp. (5%), Enterobacter ludwigii (2%) and Kocuria tytonicola (2%). TM showed that Ca, Na, B and Ni increased their concentrations in Winter while maxima of P, Zn, Cu and Fe were found in muscles of fish sampled in Summer. This study highlighted that the fish intestinal microbiota and metal composition of the fillet reflected the seasonal aquatic environmental variability.

scholarly journals Assessment of the nitrification process in a culture of pacific white shrimp, using artificial substrate and bacterial inoculum in a biofloc technology system (BFT)

2019 ◽  
Vol 49 (6) ◽  
Author(s):  
Nathalia Brenda Veiga dos Santos ◽  
Plínio Schmidt Furtado ◽  
Dionéia Evangelista César ◽  
Wilson Wasielesky Junior
Keyword(s):  
Heterotrophic Bacteria ◽  
Pacific White Shrimp ◽  
Production Performance ◽  
Artificial Substrates ◽  
Nitrifying Bacteria ◽  
Clear Water ◽  
Technology System ◽  
Working Volume ◽  
Significant Difference ◽  
Nitrification Process

ABSTRACT: Litopenaeus vannamei is the most cultured marine shrimp in all types of systems including the Bioflocs Technology System (BFT). Bioflocs are formed by microorganisms, among these, autotrophic bacteria are responsible for the nitrification process. This study aimed to identify and promote the development of nitrifying bacteria by adding artificial substrates and biofloc inoculum in L. vannamei culture in a BFT system. The experiment consisted of four treatments with three replics (4x3) as follows: (1) Control: clear water in which bioflocs were formed; (2) IN (10%): clear water with biofloc inoculum (10%); (3) IB: clear water with substrate (immature “bioballs”); and (4) MB: clear water with mature “bioballs” inoculum from a recirculation system. Treatments were stocked with shrimp juveniles (4.92±0.45 g) in 12 tanks with 200 L working volume at a stocking density of 200 shrimp/m³. Shrimps were fed twice a day with a commercial feed (38% CP) following a feeding table, and daily observations intake were made over the four weeks of the experiment. Biofloc and “bioballs” samples were collected to detect the growth of the population of nitrifying and heterotrophic bacteria by FISH. There was no significant difference between treatments (P>0.05) for survival, obtaining mean values greater than 88%. The IN (10%) treatment had lower concentrations of ammonia and nitrite, and nitrate concentration increased, while MB had a higher weight and biomass final, productivity, weekly weight gain and lower conversion of apparent feed for production performance results.

Nitrification as a source of soluble organic substrate in biological treatment

1994 ◽  
Vol 30 (6) ◽  
pp. 1-8 ◽  
Author(s):  
Bruce E. Rittmann ◽  
John M. Regan ◽  
David A. Stahl
Keyword(s):  
Kinetic Analysis ◽  
Organic Compounds ◽  
Biological Treatment ◽  
Heterotrophic Bacteria ◽  
Theoretical Work ◽  
Organic Substrate ◽  
Nitrifying Bacteria ◽  
Organic Substrates ◽  
Soluble Microbial Products ◽  
Microbial Products

In complex, multispecies populations, exchange of substrates can be an important beneficial interaction. Prior experimental and theoretical work has led to the hypothesis that the formation of soluble microbial products (SMP) by nitrifying bacteria can provide a supplementary organic substrate for heterotrophic bacteria, thereby augmenting their accumulation and stability, especially when inputs of organic substrates are low. In this study, chemostat experiments carried out with a NO2−-oxidizing strain (Nitrobacter sp.) and an NH4+-oxidizing strain (Nitrosomonas europaea) demonstrated that both nitrifiers produce SMP that can support heterotrophic bacteria. The first evidence was the presence of significant concentrations of soluble COD in the chemostat effluent, even though the influent was free of organic compounds. Second, a small heterotrophic population was maintained, apparently through utilization of the nitrifier-produced SMP. A preliminary kinetic analysis suggested that SMP kinetic parameters can be adapted from parameters measured for heterotrophs.

Characterization of bacterial biofilm communities in tertiary treatment processes for wastewater reclamation and reuse

2008 ◽  
Vol 58 (5) ◽  
pp. 1023-1030 ◽  
Author(s):  
Tadashi Shoji ◽  
Shuichi Ochi ◽  
Masaaki Ozaki
Keyword(s):  
Urban Areas ◽  
Heterotrophic Bacteria ◽  
Bacterial Biofilm ◽  
Nitrifying Bacteria ◽  
Water Supply Systems ◽  
Ammonia Oxidizing Bacteria ◽  
Tertiary Treatment ◽  
Treatment Processes ◽  
Related Group ◽  
Biofilm Development

The concern with wastewater reuse as a sustainable water resource in urban areas has been growing. For the reclamation and distribution of wastewater, biofilm development deserves careful attention from the point of view of its promotion (e.g. biofiltration) and inhibition (e.g. clogging and hygiene problems). As the first step to control biofilm development, bacterial biofilm communities in tertiary treatment processes were characterized by using molecular biological methods. The result of clone library analysis showed that Nitrospirae-related (nitrite-oxydizing bacteria) and Acidobacteria-related (probably oligotrophic bacteria) groups were dominant. The ratio of the Nitrospirae-related group to the Acidobacteria-related group was associated with ammonia load, whereas other operational conditions (process, media, temperature, salt) did not clearly affect the phylum-level community or the dominant sequence of nitrifying bacteria. The result of real-time PCR also indicated that high ammonia load promotes the proliferation of nitrite- and ammonia-oxidizing bacteria. Regarding water supply systems, some researchers also have suggested the dominance of Nitrospirae- and Acidobacteria-related groups in biofilm formed on water distribution pipes. In tertiary wastewater treatment, therefore, it is concluded that oligotrophic and autotrophic bacteria are the dominant groups in biofilm samples because assimilable organic carbon is too poor to proliferate various heterotrophic bacteria.

scholarly journals Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor

Revista Fitos ◽  
2020 ◽  
Vol 14 (01) ◽  
pp. 45-55
Author(s):  
Simone Da Silva ◽  
Danielle Cardoso Alencar ◽  
Paulo José Coelho Benevides ◽  
Spartaco Astolfi-Filho
Keyword(s):  
Natural Populations ◽  
Root Induction ◽  
Relative Area ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Mean Values ◽  
Solid Media ◽  
Medicinal Properties ◽  
Semi Solid

Psychotria ipecacuanha, is a plant species with known medicinal properties that is critically endangered due to overexploitation of natural populations. Although the difficulties in conventional propagation by seed and by vegetative propagation are generally understood, the present study enhances our knowledge by describing efficient plant regeneration and root induction protocols for P. ipecacuanha while comparing alkaloid content (emetine and cephaeline) in in vitro-derived tissues. Stem node explants were cultured on MS medium MS supplemented with indolbutiric acid (IBA) in semi-solid media and the RITA® temporary immersion bioreactor. The highest root formation (81%) was in MS + 1.5 mg L−1 IBA in the bioreactor. After 24 months of acclimatization, the plants cultivated in MS + 0.50 and 1.0 mg L-1 of IBA had the highest number of roots (3), with mean values of 10.47 and 9.40 cm, respectively. The cultures coming from 1.0 mg L−1 and 0.5 mg L−1 IBA in the bioreactor contained higher cephaeline content, with a relative area of 14.2 and 14.9%, respectively. For emetine, the 1.0 mg L−1 IBA cultures in the bioreactor, 0.5 mg L−1 IBA and MS0 cultures contained higher content than the other treatments, with a relative area of 10.2, 10.2 and 10.1%, respectively.

scholarly journals Iko River estuary: Oil exploration and the microbial community shift

2021 ◽  
Vol 10 (3) ◽  
pp. 025-032
Author(s):  
Augustine A. Unimke ◽  
Abiye A. Ibiene ◽  
Phillip O. Okerentugba
Keyword(s):  
Niger Delta ◽  
Heterotrophic Bacteria ◽  
River Estuary ◽  
Sample Collection ◽  
Mean Values ◽  
Community Shift ◽  
Niger Delta Region ◽  
Significant Difference ◽  
The Mean ◽  
Total Fungi

The continuous input of petroleum-based and other industrial pollutants along with heightened navigational activities in the inland and coastal waters of the Niger Delta region of Nigeria has contributed tremendously towards petroleum pollution of the aquatic environment. Standard analytical methods were employed in sample collection and analysis. The results showed the mean values of Total Heterotrophic Bacteria [THB] for tidal water 1.44±0.20 (x107), 1.42±0.62 (x107)and 1.82±0.61 (x107)for upstream, midstream and downstream respectively, while the mean values for CUB 1.06±0.12 (x106), 1.30±0.54 (x106) and 1.28±0.46 (x106) for upstream, midstream and downstream respectively. Similarly, the mean values for Total Fungi (TF) and Crude oil-Utilizing Fungi (CUF) were 1.08±0.12 (x106), 1.12±0.21 (x106), 1.18±0.20 (x106) and 8.2±0.78 (x104), 9.2±0.20 (x104), 8.8±0.26 (x104) for upstream, midstream and downstream respectively. For intertidal water, the mean values obtained for upstream, midstream and downstream were 1.24±0.82 (x107), 1.77±0.57 (x107) and 1.40±0.32 (x107) for THB, 1.08±0.92 (x106), 1.08±0.22 (x106) and1.13±0.21 (x106) for CUB, 1.00±0.60 (x107), 1.26±0.30 (x106) and 1.11±0.18 (x106) for Total fungi [TF] and 7.2±0.81 (x104), 9.6±0.4 (x104), 9.0±0.27 (x104) for CUF). While the values for benthic sediment were 1.55±0.38 (x108), 1.68±0.32 (x108), 2.24±0.34 (x108) for THB, 1.14±0.32 (x107), 1.24±0.88 (x107), 1.48±0.90 (x107) for CUB, 1.12±0.31 (x107), 1.20±0.52 (x107), 1.40±0.16 (x107) for TF and 8.2±0.12 (x105), 6.2±0.43 (x105), 1.01±0.12 (x106) for CUF. The results showed that there was no significant difference (p> 0.05) in the mean values of each physicochemical parameter across the different microhabitats and stations. This result revealed the massive impacts of anthropogenic gradients on the biology and physicochemistry of Iko River estuary.

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