scholarly journals The study of the share of tissues in bovine carcass parts under the influence of the flaxseed diet

2020 ◽  
Vol 36 (2) ◽  
pp. 155-165
Author(s):  
Maja Petricevic ◽  
Dragan Niksic ◽  
Nikola Delic ◽  
Tamara Stamenic ◽  
Veselin Petricevic ◽  
...  
Keyword(s):  
Dietary Supplement ◽  
Final Stage ◽  
Control Group ◽  
Flax Seed ◽  
Initial Weight ◽  
Experimental Animals ◽  
Simmental Cattle ◽  
Flax Seeds ◽  
Experimental Group ◽  
Tissue Fraction

The aim of the experiment was to investigate the effect of adding flax seed to the cattle diet in the final stage of fattening. A total of 30 male Simmental cattle of uniform initial weight were selected for the trial, which were divided into 2 groups (KON (control) and LS (experimental)). Animals in the control group did not consume flax seed as a dietary supplement, and animals in the experimental group consumed flax seeds in the amount of 3.75% of the concentrated portion of the meal in the last 90 days of fattening, i.e 300 g per day. After slaughtering and cooling, the left carcass side was cut into basic parts according to the Regulation. The study included examination of the tissue fraction of parts of the carcass of the young, determined by dissection. The results of the study showed that the addition of flax seed in the diet had no statistically significant effect on the composition of the carcass parts of the young bulls at the end of the experiment.

scholarly journals Study of fattening and slaughter traits of cattle under the influence of flax seed based nutrition

2019 ◽  
Vol 35 (2) ◽  
pp. 179-189
Author(s):  
Maja Petricevic ◽  
Dusan Zivkovic ◽  
Ostojic Andric ◽  
Dragan Niksic ◽  
Veselin Petricevic ◽  
...  
Keyword(s):  
Final Stage ◽  
Food Supplement ◽  
The Body ◽  
Flax Seed ◽  
Feed Conversion ◽  
Animal Group ◽  
Young Cattle ◽  
Flax Seeds ◽  
The Impact ◽  
Experimental Group

The trial was designed in order to examine the impact of flax seed in the nutrition of young cattle/bulls, in the final stage of the fattening. In the trial, 30 bulls of Simmental breed of uniform initial weight were selected, divided into 2 groups (control and experimental). The control animal group did not consume flax seed as a food supplement. Cattle of the experimental group consumed flax seed in an amount of 3.75% (300 g per day) of concentrated meal in the last 90 days of fattening, i.e. 300 g per day. The study included the examination of the fattening performance, slaughter traits and the composition of the bovine carcass. After slaughtering, warm carcass sides, with and without kidneys, were measured individually. Subsequent to period of cooling, the left carcass side it was cut into the main carcass parts according to the Rulebook. The results of the study showed that the addition of flax seed in the diet did not have a statistically significant effect on the body weight of bulls at the end of the trial. It was found that the addition of flax seed in the feed during the final stage of fattening did not have an impact on the differences in the average overall gain of bulls and the feed conversion ratio. Based on the data obtained by cutting of carcass sides to main parts, it was established that feeding with flax seeds had no significant effect on the share of carcass parts.

scholarly journals Impact complexes nanokarboksylativ and drug Kvatronan-Se on hematological parameters of blood of experimental animals

2017 ◽  
Vol 19 (74) ◽  
pp. 123-126
Author(s):  
N. B. Seba ◽  
M. A. Khomenko
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
Live Weight ◽  
Control Group ◽  
Experimental Animals ◽  
Group I ◽  
Control Research ◽  
Research Experiment ◽  
The Village ◽  
Experimental Group

In this paper present the results of research of influence of the preparation «Kvatronan-Se» and carboxylates of Se, Cu, Mn, Cr, Ge in different combinations on haematological parameters of blood of experimental animals. Study was carried out on the basis of PC Haleks-Agro. It is located in the village Gul'sk of Novograd Volynskiy district of Zhytomyr region. In the farm to bred of Simmental cows. For the experiment was formed four groups. We took into account age, live weight of 650–700 kg and the average milk yield 5000–6000 kg. Animals is located in identical conditions of feeding and maintenance. Research experiment was carried out on animals couples counterparts.  Preparations introduced animals three times at 10–12 days after insemination. The cows first experimental group were injected complex nanokarboksylativ Ge, Cu, Mn and Cr, second – drug Kvatronan–Se and animals third experimental group was administered a complex composed of Se, Cu, Mn, Cr. On 13 day after insemination in experimental animals were taken blood from tail vein and examined the contents of hematological parameters. Established that injection nanokarboksylativ enhance the fertility of cows in the first and third group, the figure rose to 20% in the second by 40% compared with the control. Research hematological parameters blood  animals shows that after entering content drugs of blood cells had minor changes but remained within the physiological norm. The results show that in  concentration monocyte the second group  was at 5.53% (P > 0.01) higher compared to the control group and 2.3% compared to the first and third. The content of lymphocytes in this group decreased by 26.3% (P > 0.05), 22% and 16% relative to the control, the first and third groups. Also, it should be noted that the hemoglobin in the experimental groups decreased in group I by 7.9% in the II – 3.9% and III by 7.3% compared with the control group. Also, these groups distinguished downward trend eosinophils 1.8%, 1.5%, 2.05%. Then, as the number of segment-nuclear neutrophils contrast, in the first group increased by 18.2% in the second and third at 16.9% comparatively control. The number of monocytes in the second group doslfidniy the suspicion was higher by 66.3% and 0.3% higher than the physiological norm – it may indicate a high immune response.

Observations on the role of nutrition in coat shedding in cattle

1958 ◽  
Vol 50 (1) ◽  
pp. 110-112 ◽  
Author(s):  
N. T. M. Yeates
Keyword(s):  
The Other ◽  
Control Group ◽  
Experimental Animals ◽  
Normal Colour ◽  
Experimental Group

The coat changes of an experimental group of four undernourished Shorthorn heifers have been compared with those of a control group of four well-fed heifers.Low nutrition impeded seasonal shedding in three of the four experimental animals, and the hair which was retained into the summer months appeared lifeless and bleached. One animal's hair and shedding were normal, despite low nutrition.Two animals of the control group also partially retained their hair in the summer, but it was of normal colour, good lustre, and vital appearance.It is concluded that plane of nutrition influences shedding, though some individuals within a breed are less affected than others. Two distinct varieties of coat retention were observed: one was of nutritional origin, the other inherent. Possible explanations of the differences are suggested.

scholarly journals Influence of the supplement «Press-Acid» on protein-mineral metabolism and resistance of piglets

2018 ◽  
Vol 20 (83) ◽  
pp. 320-324 ◽  
Author(s):  
N.V. Chеrniy ◽  
E.V. Matsenko ◽  
Yu.A. Shchepetilnikov ◽  
Yu. V. Maslak ◽  
O.S. Machula ◽  
...  
Keyword(s):  
Blood Serum ◽  
Mineral Metabolism ◽  
The Body ◽  
Control Group ◽  
Immunological Parameters ◽  
Oxidative Reaction ◽  
Experimental Animals ◽  
Growth Energy ◽  
First Time ◽  
Experimental Group

The aim of this study was to determine the effect of supplement «Press-Acid» on some parameters of mineral and protein metabolism and resistance of piglets. The study was done in condition «Meta» on two groups of piglets of the F-1 (BWхL) genotype. There were determined indexes of bacterial and lysocim activity of blood serum, calcium and phosphorus in piglets at the age of 21–30–45–75 days. Effect of supplement on the growth rate, morbidity and safety of pigs were revealed. We used hygienic, zootechnical, hematological, biochemical, immunological and statistical methods of analysis. The influence of «Press-Acid», which consist of formic, lactic, citric and fumaric acids, was established for the first time. The concentration of calcium in blood serum of experimental group of piglets were lower than in animals of control group. The level of phosphorus increased in blood serum of experimental animals on 11.83% (30 day), on 3.74% (45%), on 17.65% (75 day) respectively. Concentration of hemoglobin and the amount of erythrocytes increased on 11.8% and on 1.82% (30 day) in animals that feed the supplement. There were established the increase of viability of the pigs with gastro-intestinal diseases which were given the supplement in comparison with that, who did not feed it. Severity of diseases was determined using the Melenberg coefficient which did not higher than 1.2–2.4 score, it was lower on 6,0–9,3 unit than in control group. Using the supplement allowed to increase the intensity of growth of piglets by 3.7% (45 day) and 9.4% (75 day) respectively. Concentration of total protein, albumines and globulins increased on 8.66%, 9.90%, 7.3% respectively. The activity of alaninaminotransphaerasae in the blood serum of experimental animals were lower on 3.45% than in control piglets. Lysocym and bacterial activity of blood serum were higher in experimental group of piglets. Feeding piglets with addition supplement Press Acid promotes higher grow rates in experimental animals, that can be explained increases of oxidative-reducing processes in them organism. Feeding piglets with acidifier «Press-Acid», «Nifulin», zinc oxide, probiotics «Lactisan Complex» promotes growth energy, increase of morphological, biochemical and immunological parameters which indicates a higher level of oxidative reaction in the body. A low coefficient of Melenberg is recorded in the piglets at the age of 1 day. Safety was within 92.1–97.0%. That indicates a more successful elimination of complexes of protein, mineral substances, humoral protection in blood of experimental group of animals.

scholarly journals Effects of peat feeding on the performance and health status of fattening pigs and environmentally derived mycobacteria

2012 ◽  
Vol 51 (No. 12) ◽  
pp. 533-543 ◽  
Author(s):  
M. Trckova ◽  
Z. Zraly ◽  
L. Matlova ◽  
V. Beran ◽  
M. Moravkova ◽  
...  
Keyword(s):  
Health Status ◽  
Lymph Nodes ◽  
Control Diet ◽  
Live Weight ◽  
Control Group ◽  
Large White ◽  
Experimental Animals ◽  
And Performance ◽  
Short Time ◽  
Experimental Group

The purpose of the present study was to investigate the effects of feeding peat as a supplement in the period after weaning on the performance and health status of pigs. Also to assess the risk of the development of tuberculous lesions in the lymph nodes and parenchymatous organs, caused by conditionally pathogenic mycobacteria present in peat. Twenty Large White × Landrace pigs in equal numbers of barrows and gilts (mean live weight 18.0 ± 1.7 kg) were used in the experiment. The experimental group was fed a diet containing commercial underground peat in the dose of 80 g peat/kg dry matter for 30 days. Subsequently, they were fed an identical diet with the control group without peat for 60 days. A short-time feeding peat did not significantly affect the growth and performance of pigs. From day 21, a statistically highly significant (P < 0.01) increase in the consumption of the experimental diet was recorded, however, without a positive effect on the growth of experimental animals. The conversion of the peat containing diet was comparable to the conversion of the control diet. It follows from the results of biochemical analysis of blood that peat feeding for 30 days did not adversely affect the metabolic profile and health status of experimental animals. No tuberculous or tuberculoid lesions in lymph nodes or parenchymatous organs were detected in any of 20 slaughtered animals. Despite that, mycobacteria were isolated from 10 (25.0%) tissues of 5 (50.0%) pigs from the experimental group. One isolate was identified as Mycobacterium avium subsp. hominissuis (IS901– and IS1245+). Seven of nine isolates were determined as conditionally pathogenic atypical mycobacteria: M. fortuitum (n = 2) and M. xenopi (n = 5). It follows from the present results that feeding of a peat supplemented diet to pigs may be considered as economically non-effective and due to the findings of mycobacteria as risky.

scholarly journals Experimental pleural empyema model in rabbits: Why, how and what are the next steps

2014 ◽  
Vol 71 (5) ◽  
pp. 491-498 ◽  
Author(s):  
Vlado Cvijanovic ◽  
Danilo Vojvodic ◽  
Dragan Djurdjevic ◽  
Milena Jovic ◽  
Vojkan Stanic ◽  
...  
Keyword(s):  
Pleural Fluid ◽  
Ph Value ◽  
Lethal Dose ◽  
Pleural Empyema ◽  
Control Group ◽  
Mean Values ◽  
Experimental Animals ◽  
Fluid Analysis ◽  
The Mean ◽  
Experimental Group

Bacgraund/Aim. The use of new therapeutic methods to prevent development of fibrothorax as the final complication of the human pleural infections requires research with experimental animals. The aim of this study was to standardize the procedures for the establishment of our own experimental model of empyema in rabbits, since it should be able to offer similar conditions found in human pleural infections. Methods. This experiment included 15 chinchilla rabbits, weighing from 2.3 to 2.8 kg. There were 12 rabbits in the experimental group, while 3 rabbits formed the control group. On the first day, we administered 0.4-0.5 mL of turpentine in the right pleural space of the rabbits from the experimental group in order to provoke sterile exudative pleurisy. After 24 h we injected 1 mL of Staphylococcus aureus and 1 mL of Escherichia coli bacteria in the same concentration of 4.5 x 108 bacteria/mL. Thoracocentesis for the pleural fluid analysis was performed 24, 48, 72, and 96 h after bacteria instillation. In these pleural samples we estimated the number of leucocytes and the values of lactate dehydrogenase (LDH), glucose and pH in pleural fluid, as well as the presence of bacteria. We did not protect the animals with antibiotics, and on the day 7 of the experiment they were sacrificed with the lethal dose of barbiturate (iv). The lung from the empyemic side of all experimental animals and the lung of one control animal were histopathologically examined. Results. A total of 4 animals had a small amount of clear pleural fluids or there was no fluid obtained with thoracocentesis 24 and 48 h after the bacteria instillation. after the bacteria instillation. In the remaining 8 rabbits 24 h after bacteria administration the mean values (? SD) of the parameters monitored were as follows: Le 34.75 ? 6.13 x 109/L, LDH 17,000 ? 4,69 U/L, glucose 1.23 ? 0.45 mmol/L, and pH 6.975 ? 0.15. The obtained values met the criteria for the evaluation of effusion as pleural empyema or complex and complicated pleural effusion (LDH > 1000 U/L, glucose < 2.31 mmol/L and pH < 7.20). Bacterial cultures were positive in 5 out of 8 first pleural samples and in only 2 samples after 48 h of bacteria administration. There was a positive correlation between the number of leukocytes and the LDH value (r = 0.071, p < 0.001), and a negative correlation between the number of leukocytes and the glucose level (r = 0.864, p < 0.001), and the leukocytes number and pH of the pleural fluid (r = 0.894, p < 0.001). The mean glucose value increased after 48 h (3.23 ? 0.44 mmol/L), and the pH value rose after 72 h (7.22 ? 0.03) which was beyond the empyema level. Conclusion. The creation of the experimental empyema model is a very delicate work with uncertain success. Its value and importance are crucial for pleural pathology research. With the intention to obtain a more empyemic pleural reaction we created a model with two different human pathogen bacteria. We generated the satisfactory results, but not as good as those contained in some of the reference literature data.

scholarly journals Morphological and radiological characteristics of experimental bone tissue regeneration

2021 ◽  
pp. 94-104
Author(s):  
E. A. Nadyrov ◽  
V. I. Nikolaev ◽  
S. I. Kirilenko ◽  
V. V. Rozhin ◽  
N. G. Maltseva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Experimental Animals ◽  
Male Wistar Rats ◽  
Group A ◽  
Bone Trabeculae ◽  
The Right ◽  
Experimental Group

Objective. To study the dynamics of reparative bone tissue regeneration in experimental animals using a native transplantation autosmix (NTA).Materials and methods. Male Wistar rats weighing 180–200 g and aged 6–7 months were used. Bone tissue defects were modelled using a cutter with a diameter of 2 mm (experimental group). A similar defect of the middle third of the right tibia was formed in the control group animals without flling the defects with bone tissue. The animals were removed from the experiment on days 3, 7, 14, 30 and 60 of the experiment (per 6 animals for each observation period). The indicators of defect occupancy, necrosis, the area of bone trabeculae, the area of granulation tissue were determined in the histological sections. All the indicators were presented as percentage of the tested area. Osteoblasts, osteocytes, and osteoclasts were calculated in absolute values on the tested area.Results. The study has showed a higher rate of bone tissue formation in the bone defects in the experimental animals after autotransplantation of the bone mixture. Bone recovery in the experimental group had a higher rate, which was manifested by faster flling of the bone tissue defect, a low percentage of the necrosis area, a higher area of bone trabeculae, its rapid transformation into lamellar bone tissue.Conclusion. Osteogenesis had practically completed by day 30 of the experiment. At the same time, in the control group it had completed by day 60. The results obtained are a theoretical precondition for further research in the feld of bone autoplasty. Advanced development of surgical and minimally invasive technologies of bone mixture application will increase the effciency of modern reconstructive surgery of bones and joints.

scholarly journals Dynamics of blood morphological indicators of Wistar line rats under parenteral BLV infection

2021 ◽  
pp. 53-58
Author(s):  
A. V. Krasnikov ◽  
A. S. Belyakova ◽  
E. S. Krasnikova
Keyword(s):  
Aplastic Anemia ◽  
Wistar Rats ◽  
Lymphocytic Leukemia ◽  
Control Group ◽  
Experimental Animals ◽  
Laboratory Rats ◽  
Intraperitoneal Infection ◽  
Experimental Group

Hematological studies of Wistar rats with intraperitoneal infection of their lymphocytes from BLV-infected cows revealed markers characteristic of the leukemic process induced by the pathogen enzootic leukemia in cattle. In 75% of experimental animals, lymphocytic leukemia and neutropenia were detected. The number of lymphocytes in the blood of rats of the experimental group was 17-36 % more than in the control group, leukocytes in average by 30 %. The animals of the experimental group showed signs of erythrocyte aplasia, hemolytic or aplastic anemia. Allergy markers were observed in individual rats. This allows us to recommend an intraperitoneal method of infecting laboratory rats with suspended lymphocytes from infected livestock for rapid and informative reproduction of experimental BLV infection.

scholarly journals Features of feeding the slaughter young stock of minks with complete feed

2021 ◽  
Vol 211 (08) ◽  
pp. 42-50
Author(s):  
Vladimir Kulikov ◽  
Elizaveta Kvartnikova ◽  
Elena Krovina
Keyword(s):  
Experimental Studies ◽  
Live Weight ◽  
Control Group ◽  
Young Stock ◽  
Experimental Animals ◽  
Main Period ◽  
Alternative Type ◽  
Males And Females ◽  
Experimental Group ◽  
Plant Components

Abstract. In the domestic fur farming there is an urgent need to introduce an alternative type of feeding for young minks. The purpose of the work is to study possible of feeding commercial young minks with complete feed, consisting of animal and plant components. Methods. In a scientific and economic experiment on young sapphire minks intended for slaughter, 2 groups were formed according to the principle of analogues, 32 heads each (16 males and 16 females): I – control, II – experimental. The animals of the control group received from 2015 August 5 to September 15 a typical general economic ration in the form of a wet mash, an experimental one – a loose, complete feed (made according to a recipe developed by us, taking into account the current norms), mixed with water in a ratio of 1:2. All experimental studies were carried out using classical zootechnical, pathomorphological, histological, veterinary-sanitary and statistical methods. Results and scope. The dynamics of the live weight of the experimental minks showed that by the beginning of the main period, the males and females of the experimental group lagged behind the control animals, after switching to the main diet for slaughter, the males lag remained and the females caught up with the control animals. But the control and experimental animals did not differ in the length of the carcass. The safety of the livestock was higher in the experimental group: males – 100 %, females – 93.75 %; in control – 93.75 % and 87.5 %, respectively. According to the main productive indicator – the quality test – the skins of the control and experimental animals did not have significant differences. Thus, the development of an alternative type of feeding is quite possible, but it requires improvement in the direction of increasing the coefficient of digestibility of nutrients and adaptation of males to an uncharacteristic type of feeding. Scientific novelty. The prospects and the need to introduce an alternative type of feeding the commercial young stock of minks with full-feed compound feeds into the domestic fur farming are outlined.

scholarly journals Dynamics of morphological changes in the heart of rats after serial systemic administration of Doxorubicin

2020 ◽  
Vol 26 (4) ◽  
pp. 22-29
Author(s):  
O.H. Kostiuk ◽  
N.L. Hodovan ◽  
P.P. Gormash ◽  
I.V. Taran ◽  
D.I. Grebeniuk ◽  
...  
Keyword(s):  
Systemic Chemotherapy ◽  
Interstitial Fibrosis ◽  
Morphological Changes ◽  
Intraperitoneal Administration ◽  
Morphological Data ◽  
Control Group ◽  
Transverse Diameter ◽  
Experimental Animals ◽  
Collagen Formation ◽  
Experimental Group

Along with a good antitumor effect, Doxorubicin has a systemic effect with damage to vital organs, in particular the heart. The lack of a unified approach to dosing and the frequency of administration of Doxorubicin in the experiment prompts the search for an optimal model of Doxorubicin cardiomyopathy. The aim of the study was to develop a method of serial administration of Doxorubicin in medium therapeutic doses in an experiment and to evaluate the cardiotoxic effect of the drug. 42 female Wistar rats were included in the study. The control group consisted of 7 intact rats. The experimental group consisted of 35 rats who received systemic chemotherapy with Doxorubicin at a dose of 5 mg/kg once a week for 5 weeks. On days 7th, 14th, 21st, 28th, 35th, the hearts of experimental animals were taken for morphological examination. Histomorphometrically determined: the diameter of cardiomyocytes (in the middle part) and the transverse diameter of their nucleus, the width of the interstitial space (endo- and perimysia). The data of histomorphological and histomorphometric examination of the myocardium testified that all animals of the experimental group had a circulatory disorder in the heart muscle at the level of hemomicrocirculation. Such changes led to cardiomyocyte hypotrophy, interstitial edema and fibrosis. During systemic chemotherapy, the animals showed marked changes in the myocardium, such as expansion of the endomysial zone, due to capillary congestion and edema, in comparison with animals of the intact group. At the end of the experiment, the animals of the experimental group retained the expansion of the endomysial zone, mainly due to interstitial fibrosis. Such changes indicate myocardial hypoxemia with damage and death of cardiomyocytes, activation of interstitial and replacement collagen formation. The obtained morphological data indicate the development of dilated cardiomyopathy in experimental animals. Serial intraperitoneal administration of Doxorubicin at a dose of 5 mg/kg once a week for 5 weeks causes morphological changes in the myocardium of experimental animals, similar to changes in the heart of people undergoing chemotherapy with this drug.

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