scholarly journals Effects of genotype and bradyrhizobium inoculation on morphological traits, grain yield and protein content of soybean varieties

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 911-925
Author(s):  
Vladimir Miladinovic ◽  
Stefan Kolasinac ◽  
Ilinka Pecinar ◽  
Biljana Kiprovski ◽  
Dragosav Mutavdzic ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Grain Yield ◽  
Protein Content ◽  
Plant Height ◽  
Total Protein ◽  
Morphological Traits ◽  
Crop Production ◽  
Total Protein Content ◽  
Plant Seed ◽  
The Impact

Soybean crop production in Serbia involves seed inoculation by N-fixing bacteria just before sowing time. The main objective of the current work was to assess the impact of the genotype and inoculation on range of morphological and yield traits of soybean (Glycine max L. Merrill), as well as the total protein content. The experiment was conducted on chernozem soil, where soybean was previously grown. The six local varieties were used, where each variety was sown, in three replicates for both inoculated and non-inoculated treatment. The following morphological traits were analysed: the plant height, number of lateral branches, distance to the first pod, number of pods per plant, pods (containing seeds) weight per plant, seed weight per plant, and the total grain yield. The total protein content in seeds was determined by standard analytical method, while subtle differences in qualitative protein composition were assessed using Raman spectroscopy. The total protein content varied from 39.6 to 42.15 %. Performance of inoculation resulted in an increase of the plant height and the distance to the first pod, although not in all tested varieties. The highest and the lowest plant height values were observed for non-inoculated variety Dana (59.23cm) and Sava (80.03cm), respectively. The effect of genotype was much more expressed causing differences in almost all tested characters, except for the total protein content. However, Raman spectroscopy analyses revealed distinct discrimination among surveyed varieties, and differences between inoculated and non-inoculated plants in qualitative composition of seed proteins.

scholarly journals Grain Yield and Total Protein Content of Organically Grown Oats–Vetch Mixtures Depending on Soil Type and Oats’ Cultivar

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
Katarzyna Pużyńska ◽  
Stanisław Pużyński ◽  
Agnieszka Synowiec ◽  
Jan Bocianowski ◽  
Andrzej Lepiarczyk
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Total Protein ◽  
Seed Mass ◽  
Weather Conditions ◽  
Total Protein Content ◽  
Common Vetch ◽  
Oat Cultivars ◽  
Set Up ◽  
Organically Grown

The yield and quality of crop mixtures depend on natural and agrotechnical factors and their relationships. This research aimed to analyze the grain yield, its components and total protein content of the organically grown oat–vetch mixture on two different soils and depending on the oat cultivar. The three-year field experiment with two crop rotations was carried out. The experiment was set up in the southern Poland on two soils: Stagnic Luvisol (S.L.) and Haplic Cambisol (H.C.). One of four oat cultivars (‘Celer’, ‘Furman’, ‘Grajcar’ and ‘Kasztan’) was grown with the common vetch cv. ‘Hanka’. The results showed that the grain yield of mixtures was affected mainly by weather conditions. During the dry season, the share of vetch in the grain yield was 46% lower than in the season of regular rainfall. The share of vetch seeds in the mixture’s yield was ca. 21% higher when the mixtures were grown on the S.L. than the H.C. soil. The selection of oats’ cultivar for the mixture with vetch affected significantly the thousand seed mass and protein content in the vetch seeds, 46.2–50.4 g and 270–280 g kg−1, respectively. The mixture with Kasztan cultivar yielded the best and this oat cultivar seemed to be the most appropriate for organic conditions; however, in years with high variability of rainfall distribution its usefulness was less.

scholarly journals Synergistic Impact of Co-applied Micronized Sulfur and Nitrogen on Agronomical Traits of a Modern Spring Wheat Cultivars Grown in Alkaline Soil

2020 ◽  
pp. 1-16
Author(s):  
Sabah Morsy
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Nitrogen Use Efficiency ◽  
Total Protein ◽  
Total Protein Content ◽  
Alkaline Soil ◽  
Wheat Cultivars ◽  
Nitrogen Use ◽  
Straw Yield ◽  
Use Efficiency

Wheat (Triticum aestivum L.) uptakes only 40 to 60% of the soil available nitrogen. Sulfur deficiencies depress both nitrate uptake and nitrate reductase activity resulting in low nitrogen use efficiency. Soil and foliar supplied sulfur in combinations with foliar nitrogen were used on three of the modern and commercially grown wheat cultivars. The experiment was conducted in two consecutive growing seasons; 2017 and 2018 in a high pH soil, 8.55. Our results indicated that 714 kg ha-1 of pre-planting sulfur (SS) and spraying twice with 6.66% urea and 2.2% micronized sulfur (S1FS1N1) during stages 13 & 41 on Zadok`s scale, increased grain yield, total protein content, straw yield and plant height by 31.58, 26.09, 18.37 and 7.9% respectively. The results indicated a significant and positive impact sulfur-nitrogen combinations when applied on top of pre-planting applied sulfur. However, foliar sprayed sulfur had a more substantial effect on all traits, compared to the pre-planting sulfur or the foliar sprayed N, suggesting interference effect of the alkaline soil with the amount of sulfur recovered from the soil. When N and S foliar were applied simultaneously, a more substantial increase in grain yield, plant height, straw yield and total protein content was observed, suggesting a synergistic effect between these two elements. We attributed the positive effect of S1FS1N1 on improving photosynthates translocation from the sources to sinks. In addition to improving nitrogen use efficiency while reducing the plant content of NO−3 by optimizing the S/N ratio and reducing sulfur deficiency. Based on our results, we concluded that the foliar application of micronized S has the potential to improve the overall performance of wheat plants. Thus, we recommend enriching nitrogen and phosphate fertilizers with sulfur for alkaline soils.

scholarly journals GROWTH AND DEVELOPMENT OF SOYBEAN (Glycine max L.) TREATED WITH SODIUM AZIDE

2015 ◽  
Vol 2 (1) ◽  
pp. 632
Author(s):  
Kumala Dewi ◽  
Sudjino .
Keyword(s):  
Glycine Max ◽  
Protein Content ◽  
Chlorophyll Content ◽  
Plant Height ◽  
Sodium Azide ◽  
Total Protein ◽  
Growth And Development ◽  
Dry Weight ◽  
Total Protein Content ◽  
Glycine Max L

<p>Soybean (Glycine max L.) is one of horticultural plants in Indonesia. The demand of soybean in Indonesia is about 2,2 million ton per year, however, the productivity of soybean is still relatively low. This research was aimed to induce mutation in soybean through an application of sodium azide. Soybean seed of “Argomulyo” variety was obtained from Reserach Station for Leguminosae and Tuberous plant in Malang. Seeds were soaked in sodium azide of 0 mM (control), 5 mM, 10 mM or 20 mM for 12 hours. The pH of sodium azide solution was adjusted to 3 by adding sulphuric acid. For each treatment 100 seeds (M1) were used . Seeds were washed under tap water , germinated and grown in a polybag containing soil and compost vertilizer (3 : 1, v/v). In each polybag 4 seeds were germinated. All plants were watered regularly every other day. Growth and development parameters observed were plant height, chlorophyll content, time to flowering, sillique number per plant, seed dry weight and total protein content. Data were analyzed by Analysis of Variant and followed by DMRT (Duncan’s Multiple Range Test) at significant level of 5%. The results showed that sodium azide of 5 mM, 10 mM or 20 mM decreased the average of plant height 15 to 20% compared to control plants. Time to flowering, chlorophyll content, sillique number per plant, and seed dry weight were not affected by sodium azide. The average of total protein content in seeds of plant originated from seeds treated with sodium azide was 40% and this value is 5% higher compared to seeds of control plants. This seeds are potential to be further replanted and reselected for better characters.</p><p><br /><strong>Keywords</strong>: soybean (Glycine max L.), sodium azide, chlorophyll contenyt, total protein</p>

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Total protein content and protein synthesis within pre-elongation stage bovine embryos

1998 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
J.G. Thompson ◽  
A.N.M. Sherman ◽  
N.W. Allen ◽  
L.T. McGowan ◽  
H.R. Tervit
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Bovine Embryos

Yield or profit: alternatives to the development of national crop production

Ekonomika APK ◽  
2021 ◽  
Vol 316 (2) ◽  
pp. 15-25
Author(s):  
Dmytro Zherlitsyn ◽  
Andrii Skrypnyk ◽  
Nataliia Klymenko ◽  
Kateryna Tuzhyk
Keyword(s):  
Grain Yield ◽  
Crop Production ◽  
Maximum Yield ◽  
Target Function ◽  
Optimization Methods ◽  
Econometric Analysis ◽  
Practical Significance ◽  
Moderate Increase ◽  
Production Research ◽  
The Impact

The purpose of the article is to determine with the help of econometric and optimization methods the priority strategies of agrarian business in the field of crop production and to compare with the existing leaders in the use of innovations in the field of crop production. Research methods. The study is based on the use of econometric analysis methods to build trends in grain yield dynamics in leading countries in the use of innovative agricultural technologies and optimization methods for the study of dominant strategies used by agricultural enterprises in crop production. Research results. As a result of using the declining marginal grain yield depending on the amount of costs, which are determined by the cost of importing technology per 1 ha, the optimal cost values for both maximum yield and maximum profit. It is shown that at certain time intervals the costs of farmers were excessively high not only in terms of profit optimization but also to optimize yields. It is assumed that taking into account the latest innovation trends, agribusiness will move to a strategy of profit maximization Scientific novelty. As a result of econometric analysis it is shown that the use as a target function of yield leads to a significant increase in the variability of this indicator, while a moderate increase in yield is accompanied by significantly less variability. Quantitative indicators of the impact of climate risks on grain yields in Ukraine have been obtained, which explain approximately 50% of the variance in the grain yield indicator in Ukraine. Practical significance. It is shown that the representation of marginal yield in the form of a decreasing linear function is confirmed in practice. Further research, which in the presence of detailed information on the production processes of individual enterprises can be conducted by panel regression (observation points are spaced in space and time), can provide a more detailed picture of the efficiency of individual production components in their areas of interaction. Tabl.: 4. Figs.: 3. Refs.: 19.

Improving Soybean Production Using Light Supplementation at Field-Scale: A Case Study

2021 ◽  
Vol 9 (3) ◽  
pp. 259
Author(s):  
Ernane M Lemes ◽  
Breno N R Azevedo ◽  
Matheus F I Domiciano ◽  
Samuel L Andrade
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Crop Production ◽  
High Efficiency ◽  
Field Scale ◽  
Soybean Crop ◽  
Illumination System ◽  
Progress Curve ◽  
Commercial Area ◽  
Yield Responses

In modern agriculture, there is a growing need for increasing crop efficiency while minimizing environmental impacts. The use of high-efficiency light supplementation to enhance plant development is limited for high-productive crops at field conditions (outdoor). This study evaluated the soybean plant’s yield responses in an open commercial area (field scale) cultivated under conditions of artificial light supplementation. A commercial irrigated (pivot) area received an illumination system for light supplementation (LS) in its inner pivot spans. About 40 hours of LS were applied to the plants during the soybean crop cycle. The area’s outer pivot spans did not receive light supplementation (nLS). The internode number, the plant height, the pods per plant were evaluated weekly to compute the area under the progress curve (AUPC). The grain yield at harvest was also assessed. The AUPC of the internode number, plant height and pods per plant were positively affected by the LS treatment. The regular soybean cycle (nLS) is about 17 weeks; however, the LS harvest occurred three weeks later. Light supplementation increased soybean grain yield by 57.3% and profitability by 180% when compared to nLS. Although light supplementation at field scale poses a challenge, it is now affordable since sustainable field resistant technologies are now available. The present study is the first known report of light supplementation used to improve soybean crop production at field scale.

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

Development and regression of exercise-induced cardiac hypertrophy in rats

1979 ◽  
Vol 236 (2) ◽  
pp. H268-H272 ◽  
Author(s):  
R. C. Hickson ◽  
G. T. Hammons ◽  
J. O. Holoszy
Keyword(s):  
Cytochrome C ◽  
Protein Content ◽  
Total Protein ◽  
Female Rats ◽  
Heart Weight ◽  
Total Protein Content ◽  
Mitochondrial Marker ◽  
Time Intervals ◽  
Control Value ◽  
Exercise Induced

Adult female rats were exercised by daily swimming. All the increase in heart weight induced by the exercise occurred within 14 days and averaged 30%. The half times of the increases in heart weight and total protein content were about 4.5 days, whereas that of cytochrome c, which was used as a mitochondrial marker, was 6.5 days. The total amounts of DNA and of hydroxyproline in the heart, which were used to evaluate the degree of connective tissue hyperplasia, increased only slightly (8% and 10%, respectively). Other animals were subjected to the same swimming program for 21 days. Groups of rats were killed at various time intervals after stopping exercise. Heart weight, total protein content, and total cytochrome c content decreased rapidly initially, with 60% of the total regression of hypertrophy occurring during the first week. Thereafter, heart weight fell more gradually toward the sedentary control value. The hydroxyproline content of the heart, which was increased 10%, did not decrease during the regression of the hypertrophy.

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