scholarly journals STRUKTUR MIKROSKOPIS KARTILAGO EPIFISIALIS TIBIA FETUS MENCIT (Mus musculus L.) DARI INDUK DENGAN PERLAKUAN KAFEIN

2006 ◽  
Vol 12 (1) ◽  
pp. 69-74
Author(s):  
Heri Budi Santoso
Keyword(s):  
Growth Plate ◽  
Histological Structure ◽  
Developmental Defect ◽  
Cartilage Calcification ◽  
Proliferative Zone ◽  
Pregnant Mice ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
And Cartilage ◽  
Tibial Growth Plate

Caffeine affects activity of enzyme of polimerase DNA, induce the mitosis of cells mammal of before replication DNA ended the perfection, and also pursue the activity of enzyme fosfodiesterase is hence anticipated by a potential caffeine generate the developmental defect, for example can pursue the process of ossification endochondralis in growth plate. This present research studied the effect of caffeine gift by oral on pregnant dam during organogenesis to structure of histologi growth plate tibia foetus. Twenty four pregnant mice (6 per group) were treated by gavage with 0 (control), 40, 80, 120 mg/kg b.w caffeine from gestation day 6 to 15. On day 18 of pregnancy, fetuses was removed from dams by caecarean section.. Observation of histological structure of the tibial growth plate preparation by paraffin method (Hematoxylin-Eosin staining). The result showed that caffeine cause slightened proliferative zone, maturation zone, and cartilage calcification zone on the mouse tibial growth plate.

scholarly journals Analisis Pertumbuhan Kartilago Epifisialis Os Tibia Fetus Mencit (Mus musculus L.) Swiss Webster Setelah Induksi Ochratoxin A Selama Periode Organogenesis

2018 ◽  
Vol 4 (1) ◽  
pp. 25-31
Author(s):  
Arum Setiawan ◽  
Mammed Sagi ◽  
Widya Asmara ◽  
Istriyati Istriyati
Keyword(s):  
Key Words ◽  
Ochratoxin A ◽  
Growth Plate ◽  
Mus Musculus ◽  
Epiphyseal Cartilage ◽  
Maturation Zone ◽  
Proliferative Zone ◽  
Key Words Ochratoxin A ◽  
Pregnant Mice ◽  
Tibial Growth Plate

The aims of this study were determined the effects of Ochratoxin A (OTA) on growth of fetus tibia epiphyseal cartilage during organogenesis period. Twenty four pregnant mice were divided randomly into 4 groups of 6. Ochratoxin A was dissolved in sodium bicarbonateand administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining were used as control. The fetal tibia was taken after the 18 th day of pregnancy. The growth of tibia epiphyseal cartilages were observed histologically using Erlich’s Haematoxylin-Eosin Stain. The result of this study indicated that OTA caused decreased thickness of the rest zone, proliferative zone, maturation zone and calsification zone of the fetus tibial growth plate significantly. Key words: Ochratoxin A, tibia, cartilage, and thickness.

An ultrastructural study of mitotic chondrocytes in the proliferative zone of the rat tibial growth plate

1993 ◽  
Vol 175 (1) ◽  
pp. 41-45 ◽  
Author(s):  
S. Shibata ◽  
O. Baba ◽  
M. Niikura ◽  
S. Suzuki ◽  
Y. Yamashita ◽  
...  
Keyword(s):  
Growth Plate ◽  
Ultrastructural Study ◽  
Proliferative Zone ◽  
Tibial Growth Plate

scholarly journals Anatomical and Histological Structure of Black Pomfret Fish Kidney (Formio niger)

2017 ◽  
Vol 1 ◽  
pp. 71-74
Author(s):  
Nurul Safitri Apriliani
Keyword(s):  
Growth Rate ◽  
Fish Species ◽  
Economic Value ◽  
Histological Structure ◽  
Lymphoid Tissues ◽  
The Many ◽  
Fish Kidney ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Fast Growth Rate

Black pomfret is one of the many fish species found in the southern sea. Pomfret has high economic value and is an active swimmer that has fast growth rate, resistant to disease, and easy to maintain. The purpose of this research was to know the structure of anatomy and histology of black pomfret’s kidney. Histological slides were made using paraffin method and stain with Hematoxylin-Eosin. The results showed that kidney of black pomfret have a soft texture, and the colour is red degradation brown. Histologically, the kidney is found a glomerolous, tubules and lymphoid tissues.

scholarly journals Indeks Gonadosomatik dan Struktur Histologis Gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846)

Biosfera ◽  
2017 ◽  
Vol 34 (2) ◽  
pp. 67 ◽  
Author(s):  
Luthfi Nurhidayat ◽  
Febrina Nanda Arfiani ◽  
Bambang Retnoaji
Keyword(s):  
Ovarian Follicle ◽  
Gonadosomatic Index ◽  
Native Fish ◽  
Histological Structure ◽  
Male And Female ◽  
Fish Exploitation ◽  
Males And Females ◽  
Mature Fish ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Uceng fish is wild native fish of Indonesia that live in fresh water river. It is very common that people consume the fish for it high protein contain. High demand of fish availability cause the fish exploitation increasing rapidly in nature. The high intensity of fish exploitation, soon will induce declining population of the fish and leading to extinction. Uceng fish cultivation is one alternative to solve the tread. The purpose of this research is to examine the Gonadosomatic Index (GSI) and gonad histological structure of male and female uceng fish (Nemacheilus fasciatus) on the period of immature and mature. Fish were dissected , weighed and the gonads were measured for gonadosomatic index. Moreover, gonad were fixed in NBF 5% for histological preparation by paraffin method and which than were stained by Hematoxylin-Eosin. The result were analyzed for both qualitatively and quantitatively. Qualitative analysis was conducted for gonad histological structure of male and female fish, while quantitative analysis was for Gonadosomatic Index (GSI) of fish respectively. The result showed that there were differences on the gonadosomatic index of males and females fish, the histological structure of ovarian follicle and the composition of the spermatogenic cells of immature gonads and mature gonads of the fish respectively. It was conclude that there are differences on Gonadosomatic Index and gonad histological structure on immature and mature of male and female of Uceng fish (N. fasciatus).

scholarly journals Comparative Anatomy and Histology of Black Pomfret (Formio niger) and Nile tilapia (Oreochromis niloticus) Kidney

2017 ◽  
Vol 6 (1) ◽  
pp. 9
Author(s):  
Nurul Safitri Apriliani ◽  
Muhammad Jafar Luthfi
Keyword(s):  
Oreochromis Niloticus ◽  
Lymphoid Tissue ◽  
Nile Tilapia ◽  
Comparative Anatomy ◽  
Distal Tubule ◽  
Histological Structure ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Habitat Differences ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Black pomfret and nile tilapia are belonging to order Perciformes. Both fish are live in different habitat. Black pomfret is marine water whereas nile tilapia is in freshwater. The purpose of the study was to determine anatomy and histology of the kidneys structures in both fishes. Histological preparations were done using paraffin method, with <em>Hematoxylin-Eosin</em> (HE) staining. The results, showed that Black pomfret and nile tilapia have y-shape kidney. Nile tilapia has darker red colour and softer texture than black pomfret kidney. Histologically, black pomfret and nile tilapia kidneys have a distal tubule, proximal tubule, glomerulus and lymphoid tissue. Glomerular diameter of nile tilapia (69,22 µm) was larger than pomfret (61,25 µm). It can be concluded that differences between anatomical and histological structure of kidney are affected by habitat differences.

Studi Histokimia Usus Biawak Air (Varanus salvator bivittatus) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati
Keyword(s):  
Tissue Sample ◽  
Histological Section ◽  
Tissue Perfusion ◽  
Intestinal Wall ◽  
Biological Information ◽  
Suitable Habitat ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.

Isolation, Culture and Identification of Bovine Skeletal Stem Cells

2021 ◽  
Vol 11 (12) ◽  
pp. 2337-2345
Author(s):  
Junhui Lai ◽  
Qin Yang ◽  
Ruining Liang ◽  
Weijun Guan ◽  
Xiuxia Li
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Bone Formation ◽  
Growth Plate ◽  
Bone Development ◽  
Long Bone ◽  
Biological Characterization ◽  
Self Renewal ◽  
And Cartilage

The growth plate is essential in long bone formation and contains a wealth of skeletal stem cells (SSCs). Though the origin and the mechanism for SSCs generation remain uncertain, recent studies demonstrate the transition from cartilage to bone that in the lineage for bone development. SSCs possesses the ability to differentiate into bone and cartilage in vitro. In this research, we aimed to isolate and culture the skeletal stem cells from bovine cattle and then studied its biological characterization. The results showed that these bovine SSCs are positive for PDPN+CD73+CD164+CD90+CD44+ cell surface bio-markers, they are capable of self-renewal and differentiation. Our dates proved that SSCs exists in bovine’s long bone.

scholarly journals Local Changes to the Distal Femoral Growth Plate Following Injury in Mice

2017 ◽  
Vol 139 (7) ◽  
Author(s):  
Lauren M. Mangano Drenkard ◽  
Meghan E. Kupratis ◽  
Katie Li ◽  
Louis C. Gerstenfeld ◽  
Elise F. Morgan
Keyword(s):  
Growth Plate ◽  
Skeletal Development ◽  
Global Changes ◽  
Mineral Density ◽  
Injury Site ◽  
Hypertrophic Zone ◽  
Proliferative Zone ◽  
Spatial Changes ◽  
Zonal Organization ◽  
Growth Plate Injury

Injury to the growth plate is associated with growth disturbances, most notably premature cessation of growth. The goal of this study was to identify spatial changes in the structure and composition of the growth plate in response to injury to provide a foundation for developing therapies that minimize the consequences for skeletal development. We used contrast-enhanced microcomputed tomography (CECT) and histological analyses of a murine model of growth plate injury to quantify changes in the cartilaginous and osseous tissue of the growth plate. To distinguish between local and global changes, the growth plate was divided into regions of interest near to and far from the injury site. We noted increased thickness and CECT attenuation (a measure correlated with glycosaminoglycan (GAG) content) near the injury, and increased tissue mineral density (TMD) of bone bridges within the injury site, compared to outside the injury site and contralateral growth plates. Furthermore, we noted disruption of the normal zonal organization of the physis. The height of the hypertrophic zone was increased at the injury site, and the relative height of the proliferative zone was decreased across the entire injured growth plate. These results indicate that growth plate injury leads to localized disruption of cellular activity and of endochondral ossification. These local changes in tissue structure and composition may contribute to the observed retardation in femur growth. In particular, the changes in proliferative and hypertrophic zone heights seen following injury may impact growth and could be targeted when developing therapies for growth plate injury.

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