scholarly journals Indeks Gonadosomatik dan Struktur Histologis Gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846)

Biosfera ◽  
2017 ◽  
Vol 34 (2) ◽  
pp. 67 ◽  
Author(s):  
Luthfi Nurhidayat ◽  
Febrina Nanda Arfiani ◽  
Bambang Retnoaji
Keyword(s):  
Ovarian Follicle ◽  
Gonadosomatic Index ◽  
Native Fish ◽  
Histological Structure ◽  
Male And Female ◽  
Fish Exploitation ◽  
Males And Females ◽  
Mature Fish ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Uceng fish is wild native fish of Indonesia that live in fresh water river. It is very common that people consume the fish for it high protein contain. High demand of fish availability cause the fish exploitation increasing rapidly in nature. The high intensity of fish exploitation, soon will induce declining population of the fish and leading to extinction. Uceng fish cultivation is one alternative to solve the tread. The purpose of this research is to examine the Gonadosomatic Index (GSI) and gonad histological structure of male and female uceng fish (Nemacheilus fasciatus) on the period of immature and mature. Fish were dissected , weighed and the gonads were measured for gonadosomatic index. Moreover, gonad were fixed in NBF 5% for histological preparation by paraffin method and which than were stained by Hematoxylin-Eosin. The result were analyzed for both qualitatively and quantitatively. Qualitative analysis was conducted for gonad histological structure of male and female fish, while quantitative analysis was for Gonadosomatic Index (GSI) of fish respectively. The result showed that there were differences on the gonadosomatic index of males and females fish, the histological structure of ovarian follicle and the composition of the spermatogenic cells of immature gonads and mature gonads of the fish respectively. It was conclude that there are differences on Gonadosomatic Index and gonad histological structure on immature and mature of male and female of Uceng fish (N. fasciatus).

scholarly journals Anatomical and Histological Structure of Black Pomfret Fish Kidney (Formio niger)

2017 ◽  
Vol 1 ◽  
pp. 71-74
Author(s):  
Nurul Safitri Apriliani
Keyword(s):  
Growth Rate ◽  
Fish Species ◽  
Economic Value ◽  
Histological Structure ◽  
Lymphoid Tissues ◽  
The Many ◽  
Fish Kidney ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Fast Growth Rate

Black pomfret is one of the many fish species found in the southern sea. Pomfret has high economic value and is an active swimmer that has fast growth rate, resistant to disease, and easy to maintain. The purpose of this research was to know the structure of anatomy and histology of black pomfret’s kidney. Histological slides were made using paraffin method and stain with Hematoxylin-Eosin. The results showed that kidney of black pomfret have a soft texture, and the colour is red degradation brown. Histologically, the kidney is found a glomerolous, tubules and lymphoid tissues.

scholarly journals Comparative Anatomy and Histology of Black Pomfret (Formio niger) and Nile tilapia (Oreochromis niloticus) Kidney

2017 ◽  
Vol 6 (1) ◽  
pp. 9
Author(s):  
Nurul Safitri Apriliani ◽  
Muhammad Jafar Luthfi
Keyword(s):  
Oreochromis Niloticus ◽  
Lymphoid Tissue ◽  
Nile Tilapia ◽  
Comparative Anatomy ◽  
Distal Tubule ◽  
Histological Structure ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Habitat Differences ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Black pomfret and nile tilapia are belonging to order Perciformes. Both fish are live in different habitat. Black pomfret is marine water whereas nile tilapia is in freshwater. The purpose of the study was to determine anatomy and histology of the kidneys structures in both fishes. Histological preparations were done using paraffin method, with <em>Hematoxylin-Eosin</em> (HE) staining. The results, showed that Black pomfret and nile tilapia have y-shape kidney. Nile tilapia has darker red colour and softer texture than black pomfret kidney. Histologically, black pomfret and nile tilapia kidneys have a distal tubule, proximal tubule, glomerulus and lymphoid tissue. Glomerular diameter of nile tilapia (69,22 µm) was larger than pomfret (61,25 µm). It can be concluded that differences between anatomical and histological structure of kidney are affected by habitat differences.

scholarly journals REPRODUCTIVE ASPECTS OF SCALLOPED PERCHLET, Ambassis nalua (HAMILTON, 1822) IN PABEAN BAY INDRAMAYU, WEST JAVA

2017 ◽  
Vol 9 (1) ◽  
pp. 113-124 ◽  
Author(s):  
Nisha Desfi Arianti ◽  
M.F. Rahardjo ◽  
Ahmad Zahid
Keyword(s):  
Sex Ratio ◽  
Sampling Period ◽  
Spawning Season ◽  
Diameter Distribution ◽  
Male And Female ◽  
Fish Samples ◽  
Males And Females ◽  
Mature Fish ◽  
First Maturity ◽  
Egg Diameter

ABSTRACTScalloped perchlet, Ambassis nalua,  is one of fishes in Pabean Bay, Indramayu. This research aims to determine the reproductive aspects of Ambassis nalua in Pabean Bay Indramayu including sex ratio, spawning season, first maturity, fecundity and spawning pattern. Fish samples were collected by trap net and trammel net at three sites in Pabean Bay from April to October 2015. A total of 424 of A. nalua were caught, consist of 114 males and 310 females; with total length ranged from 38.04 to 112.63 mm and total weight ranged from 0.37 to 25.44 g. Sex ratio of mature fish  was 1:1.9. The mature males and females were found in every month of sampling period. The gonado-somatic index (GSI) ranged from 0.40 to 0.83 and 2.36 to 4.54 for male and female, respectively. The peak of spawning season was found in September. The first maturity (Lm50) for male and female were 79.17 mm and 91.25 mm, respectively. The fecundity varied from 3,451–32,465 eggs. Egg diameter distribution shows that spawning pattern of A. nalua was batch spawner. Keywords: Ambassis nalua, spawning season, sex ratio, reproduction

scholarly journals STRUKTUR MIKROSKOPIS KARTILAGO EPIFISIALIS TIBIA FETUS MENCIT (Mus musculus L.) DARI INDUK DENGAN PERLAKUAN KAFEIN

2006 ◽  
Vol 12 (1) ◽  
pp. 69-74
Author(s):  
Heri Budi Santoso
Keyword(s):  
Growth Plate ◽  
Histological Structure ◽  
Developmental Defect ◽  
Cartilage Calcification ◽  
Proliferative Zone ◽  
Pregnant Mice ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
And Cartilage ◽  
Tibial Growth Plate

Caffeine affects activity of enzyme of polimerase DNA, induce the mitosis of cells mammal of before replication DNA ended the perfection, and also pursue the activity of enzyme fosfodiesterase is hence anticipated by a potential caffeine generate the developmental defect, for example can pursue the process of ossification endochondralis in growth plate. This present research studied the effect of caffeine gift by oral on pregnant dam during organogenesis to structure of histologi growth plate tibia foetus. Twenty four pregnant mice (6 per group) were treated by gavage with 0 (control), 40, 80, 120 mg/kg b.w caffeine from gestation day 6 to 15. On day 18 of pregnancy, fetuses was removed from dams by caecarean section.. Observation of histological structure of the tibial growth plate preparation by paraffin method (Hematoxylin-Eosin staining). The result showed that caffeine cause slightened proliferative zone, maturation zone, and cartilage calcification zone on the mouse tibial growth plate.

GONADOTROPHIN PATTERNS IN MALE AND FEMALE RATS:

1968 ◽  
Vol 58 (4) ◽  
pp. 600-612 ◽  
Author(s):  
Robert Boyd ◽  
Donald C. Johnson
Keyword(s):  
Ascorbic Acid ◽  
Testosterone Propionate ◽  
Female Rats ◽  
Female Rat ◽  
Feedback Effects ◽  
Male And Female ◽  
Prostate Weight ◽  
Male And Female Rats ◽  
Males And Females ◽  
Normal Males

ABSTRACT The effects of various doses of testosterone propionate (TP) upon the release of luteinizing hormone (LH or ICSH) from the hypophysis of a gonadectomized male or female rat were compared. Prostate weight in hypophysectomized male parabiotic partners was used to evaluate the quantity of circulating LH. Hypophyseal LH was measured by the ovarian ascorbic acid depletion method. Males castrated when 45 days old secreted significantly more LH and had three times the amount of pituitary LH as ovariectomized females. Administration of 25 μg TP daily reduced the amount of LH in the plasma, and increased the amount in the pituitary gland, in both sexes. Treatment with 50 μg caused a further reduction in plasma LH in males, but not in females, while pituitary levels in both were equal to that of their respective controls. LH fell to the same low level in partners of males or females receiving 100 μg TP. When gonadectomized at 39 days, males and females had the same amount of plasma LH, but males had more stored hormone. Pituitary levels were unchanged from controls following treatment with 12.5, 25 or 50 μg TP daily, but plasma values dropped an equal amount in both sexes with the latter two doses. Androgenized males or females, gonadectomized when 39 days old, were very sensitive to the effects of TP and plasma LH was significantly reduced with 12.5 μg daily. Pituitary LH in androgenized males was higher than that of normal males but was reduced to normal by small amounts of TP. The amount of stored LH in androgenized females was not different from that of normal females and it was unchanged by any dose of TP tested. Results are consistent with the conclusion that the male hypothalamic-hypophyseal axis is at least as sensitive as the female axis to the negative feedback effects of TP. Androgenization increases the sensitivity to TP in both males and females.

Studi Histokimia Usus Biawak Air (Varanus salvator bivittatus) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati
Keyword(s):  
Tissue Sample ◽  
Histological Section ◽  
Tissue Perfusion ◽  
Intestinal Wall ◽  
Biological Information ◽  
Suitable Habitat ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.

Gender Differences in Clothing Worn in Current Popular Comic Books

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Kyle Landon Jossy
Keyword(s):  
Gender Differences ◽  
Content Analysis ◽  
Comic Books ◽  
Book Industry ◽  
Comic Book ◽  
Lower Body ◽  
Female Characters ◽  
Male And Female ◽  
Males And Females

This study looked at how males and females were portrayed, based on the amount of skin shown in the clothing worn.  A Content analysis was performed on a sample of 20 randomly selected popular comics from the last 3 years.  Both male and female characters were rated on how much skin they showed in three clothing categories; neck line, sleeve length, and lower body.  Results showed that in all 3 categories, women consistently wore more revealing clothing.  The findings demonstraetd that the comic book industry is comparable to other forms of media, in the sexualization of female characters, by having them wear more revealing clothing.

scholarly journals Spinal Muscular Atrophy after Nusinersen Therapy: Improved Physiology in Pediatric Patients with No Significant Change in Urine, Serum, and Liquor 1H-NMR Metabolomes in Comparison to an Age-Matched, Healthy Cohort

Metabolites ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 206
Author(s):  
Leon Deutsch ◽  
Damjan Osredkar ◽  
Janez Plavec ◽  
Blaž Stres
Keyword(s):  
Machine Learning ◽  
Spinal Muscular Atrophy ◽  
1H Nmr ◽  
Pediatric Patients ◽  
Muscular Atrophy ◽  
Male And Female ◽  
Before And After ◽  
Healthy Cohort ◽  
Males And Females ◽  
Urine Serum

Spinal muscular atrophy (SMA) is a genetically heterogeneous group of rare neuromuscular diseases and was until recently the most common genetic cause of death in children. The effects of 2-month nusinersen therapy on urine, serum, and liquor 1H-NMR metabolomes in SMA males and females were not explored yet, especially not in comparison to the urine 1H-NMR metabolomes of matching male and female cohorts. In this prospective, single-centered study, urine, serum, and liquor samples were collected from 25 male and female pediatric patients with SMA before and after 2 months of nusinersen therapy and urine samples from a matching healthy cohort (n = 125). Nusinersen intrathecal application was the first therapy for the treatment of SMA by the Food and Drug Administration (FDA) and the European Medicines Agency (EMA). Metabolomes were analyzed using targeted metabolomics utilizing 600 MHz 1H-NMR, parametric and nonparametric multivariate statistical analyses, machine learning, and modeling. Medical assessment before and after nusinersen therapy showed significant improvements of movement, posture, and strength according to various medical tests. No significant differences were found in metabolomes before and after nusinersen therapy in urine, serum, and liquor samples using an ensemble of statistical and machine learning approaches. In comparison to a healthy cohort, 1H-NMR metabolomes of SMA patients contained a reduced number and concentration of urine metabolites and differed significantly between males and females as well. Significantly larger data scatter was observed for SMA patients in comparison to matched healthy controls. Machine learning confirmed urinary creatinine as the most significant, distinguishing SMA patients from the healthy cohort. The positive effects of nusinersen therapy clearly preceded or took place devoid of significant rearrangements in the 1H-NMR metabolomic makeup of serum, urine, and liquor. Urine creatinine was successful at distinguishing SMA patients from the matched healthy cohort, which is a simple systemic novelty linking creatinine and SMA to the physiology of inactivity and diabetes, and it facilitates the monitoring of SMA disease in pediatric patients through non-invasive urine collection.

scholarly journals Stem cell treatment improves post stroke neurological outcomes: a comparative study in male and female rats

2021 ◽  
pp. svn-2020-000834
Author(s):  
Koteswara Rao Nalamolu ◽  
Bharath Chelluboina ◽  
Casimir A Fornal ◽  
Siva Reddy Challa ◽  
David M Pinson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sex Differences ◽  
Motor Function ◽  
Infarct Volume ◽  
Female Rats ◽  
Human Umbilical Cord ◽  
Male And Female ◽  
Post Stroke ◽  
Male And Female Rats ◽  
Males And Females

Background and purposeThe therapeutic potential of different stem cells for ischaemic stroke treatment is intriguing and somewhat controversial. Recent results from our laboratory have demonstrated the potential benefits of human umbilical cord blood-derived mesenchymal stem cells (MSC) in a rodent stroke model. We hypothesised that MSC treatment would effectively promote the recovery of sensory and motor function in both males and females, despite any apparent sex differences in post stroke brain injury.MethodsTransient focal cerebral ischaemia was induced in adult Sprague-Dawley rats by occlusion of the middle cerebral artery. Following the procedure, male and female rats of the untreated group were euthanised 1 day after reperfusion and their brains were used to estimate the resulting infarct volume and tissue swelling. Additional groups of stroke-induced male and female rats were treated with MSC or vehicle and were subsequently subjected to a battery of standard neurological/neurobehavioral tests (Modified Neurological Severity Score assessment, adhesive tape removal, beam walk and rotarod). The tests were administered at regular intervals (at days 1, 3, 5, 7 and 14) after reperfusion to determine the time course of neurological and functional recovery after stroke.ResultsThe infarct volume and extent of swelling of the ischaemic brain were similar in males and females. Despite similar pathological stroke lesions, the clinical manifestations of stroke were more pronounced in males than females, as indicated by the neurological scores and other tests. MSC treatment significantly improved the recovery of sensory and motor function in both sexes, and it demonstrated efficacy in both moderate stroke (females) and severe stroke (males).ConclusionsDespite sex differences in the severity of post stroke outcomes, MSC treatment promoted the recovery of sensory and motor function in male and female rats, suggesting that it may be a promising treatment for stroke.

Mammalian exocrine secretions XIX. Chemical characterization of the interdigital secretion of the Black Wildebeest, Connochaetes gnou

2020 ◽  
Vol 75 (9-10) ◽  
pp. 339-351
Author(s):  
Barend V. Burger ◽  
Desmond Slade ◽  
Marlize Z. Bekker ◽  
Aron H. Goitom
Keyword(s):  
Chemical Characterization ◽  
Male And Female ◽  
Black Wildebeest ◽  
Connochaetes Gnou ◽  
Electron Impact Mass ◽  
Males And Females ◽  
Strong Attachment ◽  
Impact Mass ◽  
Acid Esters

AbstractUsing gas chromatography (GC) in conjunction with electron impact mass spectrometry and retention-time comparison, 94 compounds, ranging from 2-methyl-2-propenal to octadecanoic acid, were identified in the interdigital secretions of male and female black wildebeests, Connochaetes gnou (also known as the white-tailed gnu). The constituents of these secretions belong to many different compound classes, including hydrocarbons, alcohols, aromatics and aliphatic carbonyl compounds including carboxylic acids as well as carboxylic acid esters. Relatively small quantitative differences were found between the male and female interdigital secretions. It was concluded that these compounds probably do not play a significant role in territorial marking or in chemical communication between males and females of the species, but they could be involved in preserving the remarkably strong attachment between members of social subgroups in black wildebeest populations.

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