Parameters of macrophage reaction and humoral immunity in patients with implant-associated in-flammation after primary endoprosthetics of knee joint

2020 ◽  
Vol 28 (4) ◽  
pp. 421-428
Author(s):  
Elena A. Galashina ◽  
Ekaterina V. Gladkova ◽  
Veronika Blinnikova ◽  
Sergey P. Shpinyak ◽  
Vladimir Ulyanov
Keyword(s):  
Knee Joint ◽  
Humoral Immunity ◽  
Comparison Group ◽  
Risk Groups ◽  
Main Group ◽  
Control Group ◽  
Complement Components ◽  
Inflammatory Reactions ◽  
Previous Period ◽  
Macrophage Reaction

Aim. To study peculiarities of macrophage reaction and humoral immunity in patients with signs of implant-associated inflammation after the primary endoprosthetics of the knee joint. Materials and Methods. Examination of 48 patients with signs of implant-associated inflammation after the primary endoprosthetics of the knee joints (the main group) and of 44 patients without signs of inflammatory reactions (comparison group) was carried out. Control group involved 30 healthy donors without diseases of the musculoskeletal system. Results. In 1 and 12 months after the surgery, patients of the main group showed elevated levels of the factor inhibiting migration of macrophages (MIF), of macrophage stimulating protein (MSP), class A immunoglobulins (IgA), С3 and С4 complement components as compared to control, and also to each previous period. In 1 month after the surgery, the content of class M and G immunoglobulins (IgM and IgG) decreased relative to control; in 12 months after the surgery a more pronounced reduction was noted. Increase in serum concentrations of MIF, MSP, IgA, С3 and С4-complement components was noted in the comparison group in 1 and 12 months and also in comparison with the previous period. Reduction of the levels of IgM, IgG in 1 and 12 months after the operation was noted in comparison with control and with the previous period. Conclusion. A study of parameters of macrophage reaction, proteins of complement system and the main classes of immunoglobulins permits to identify signs of implant-associated inflammation in 1 and 12 months after surgery, and, consequently, to determine patients of risk groups that require additional diagnostics measures.

Thromboembolic complications prevention in the obstetric practice

2017 ◽  
pp. 19-24
Author(s):  
O.V. Grishchenko ◽  
◽  
V.V. Bobrytska ◽  
Keyword(s):  
Pulmonary Embolism ◽  
Comparison Group ◽  
Main Group ◽  
Control Group ◽  
Efficacy And Safety ◽  
Thromboembolic Complications ◽  
Moderate Risk ◽  
Obstetric Practice ◽  
Thrombotic Complications ◽  
And Control

The objective: To evaluate the clinical efficacy and safety of Enoxaparin-Pharmex for the prevention of thrombotic complications (pulmonary embolism) in the postoperative period in patients with moderate risk of these complications. Patients and methods. The study included 50 women after a caesarean section had an average degree of risk of pulmonary embolism. Patients were divided into the main group (n=25) and control group (n=25) in accordance with the treatment: patients of the main group received postoperative Еnoxaparin- Pharmex, group comparisons enoxaparin sodium (brand foreign manufacturer’s). Patients in both groups received the drug at a dose of 20 mg for 5 days, 1 time per day subcutaneously. Results. The research data analysis showed identity results of hemostasiogram of patients in the main group and the comparison group, no side effects after treatment in both groups. Conclusion. The clinical studies suggest the drug Enoxaparin-Pharmex is effective, safe LMWH, which can be used to prevent troboembolic complications, including post-operative treatment in obstetric practice. Spectrum of Enoxaparin-Pharmex can be extended to the prevention and treatment of thromboembolic conditions of varying severity with appropriate doses of the drug. Key words: Enoxaparin-Pharmex, prevention of pulmonary embolism.

scholarly journals BIOMARKERS OF OSTEO-DEFFICIENCY IN THE COURSE OF SECONDARY OSTEOPOROSIS IN PATIENTS WITH COMORBID CHRONIC PANCREATITIS AND ARTERIAL HYPERTENSION

2020 ◽  
Vol 20 (4) ◽  
pp. 16-20
Author(s):  
T.I. Viun ◽  
L.M. Pasieshvili ◽  
S.V. Viun ◽  
A.S. Marchenko ◽  
O.V. Karaya
Keyword(s):  
Chronic Pancreatitis ◽  
Acid Phosphatase ◽  
Arterial Hypertension ◽  
Comparison Group ◽  
Practical Importance ◽  
Main Group ◽  
Secondary Osteoporosis ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Detection Of Biomarkers

The prevalence of comorbidities has been growing for the last decades. Therefore, the detection of biomarkers for diagnostic and prognostic purposes is of great practical importance. The aim of this study was to assess the biomarkers of osteo-defficiency in the course of secondary osteoporosis in patients with comorbid chronic pancreatitis and arterial hypertension. We examined 110 patients with chronic pancreatitis: 70 of them had comorbid hypertension, and 40 patients were found as having no comorbidities. The age of patients ranged from 33.2 ± 2.1 (main group) and 32.9 ± 3.1 years (comparison group); women predominated (72.9% and 70%, respectively). The control group includes 78 healthy individuals of the same age and sex. Diagnostic investigation included studying clinical and anamnestic characteristics of patients (duration of the disease, manifestations of the course, frequency of recrudescence, fractures) and biochemical parameters of bone metabolism: osteocalcin, total bone phosphatase and tartrate-resistant acid phosphatase and the establishment of correlations between these parameters and incidence of complications. It was found that in the isolated course of chronic pancreatitis there is a high (R = 0.60) statistically significant (p <0.01) correlation between the levels of osteocalcin and pancreatic elastase-1. A negative statistically significant (p <0.01) mean correlation (R = -0.49) was found between the content of tartrate-resistant acid phosphatase and age of the patients having comorbidity of chronic pancreatitis and hypertension, as well as there is a moderate correlation between the content of tartrate-resistant acid phosphatase and the duration of hypertension, which is statistically significant (R = 0.36, p <0.01). The levels of total bone phosphatase and tartrate-resistant acid phosphatase in the main group exceeded the reference values in 2.5 and 1.9 times respectively (CMU; U = 866.0; p <0.01), while in the comparison group were 2 times (total bone phosphatase) and 1.3 times higher (tartrate-resistant acid phosphatase) times, respectively (CMU; U = 821.0; p <0.01) that enables to diagnose the development of osteopenic conditions. That is, the combined course of chronic pancreatitis and hypertension should be considered as unfavourable tandem in the development of secondary osteoporosis and requires early osteoporotic screening.

The optimization of reparative processes after cesarean section (clinical-experimental research)

2015 ◽  
Vol 64 (4) ◽  
pp. 4-12 ◽  
Author(s):  
Edvard Karpovich Aylamazyan ◽  
Victoriya Yuryevna Andreeva ◽  
Tatiana Ulyanovna Kuzminykh ◽  
Dmitriy Igorevich Sokolov ◽  
Sergey Alekseevich Selkov ◽  
...  
Keyword(s):  
Pregnant Woman ◽  
Cesarean Section ◽  
Comparison Group ◽  
Main Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Vascular Endothelial ◽  
Actual Direction ◽  
Uterine Scar ◽  
Endothelial Growth Factor

Development and introduction of materials influenced on reparation process in a myometrium is actual direction of modern obstetrics in connection with the increase of cesarean section rate. One of such preparation is «Collost», it stimulates the processes of neoangio- and myogenesis and excludes the disorderly growth of scars tissue. Aim: To study the influence of bioplastic material «Collost» on the reparation process in myometrium after cesarean section. Objectives: to estimate the morpho-functional state of scar on uterus after cesarean sections depending on implantation of collagen membrane. Material and methods: There were included 13 female rabbits in experimental part of the study. In clinical part of the study there were included 20 pregnant woman with intraoperative application of «Collost» (main group), 30 pregnant woman without «Collost» implantation (comparison group) and 10 pregnant with vaginal delivery (control group). Results: histological, immunohistological and immunological findings of postoperative uterine scars of female rabbits showed significant differences of expressions of miosin, vascular endothelial growth factor and collagen I type. The myometrium was significantly thicker in the area of uterine scar in main group (with collagen membrane implantation) than in comparison group. Conclusions: bioplastic material «Collost» leads to full formation of muscular wall and vascular ture in the area of postoperative uterine scar.

scholarly journals Optimization of rehabilitation measures after artificial abortion as the result of immunomodulator aminodihydrophthalasindione sodium apply in comprehensive therapy

2019 ◽  
pp. 156-161
Author(s):  
E. A. Voroshilova
Keyword(s):  
Marked Reduction ◽  
Clinical Effect ◽  
Comparison Group ◽  
Clinical Symptoms ◽  
Randomized Study ◽  
Main Group ◽  
Control Group ◽  
Comprehensive Therapy ◽  
Effect Of Treatment ◽  
Artificial Abortion

The article presents the results of a comparative randomized study, the purpose of which was to evaluate the effectiveness of the use of aminodihydrophthalasindione sodium (Galavit, LLC SELVIM, Russia) in the treatment of patients undergoing an abortion. Included in the study, 48 women were divided into two groups, 24 patients of the main group in addition to the standard rehabilitation were treated with aminodihydrophthalasindione sodium in the comparison group – 24 patients underwent only standard rehabilitation. In this study, all patients (100%) of the main group who were treated with aminodihydrophthalasindione sodium in addition to the standard therapy marked reduction of the clinical symptoms of the disease and positive dynamics was observed at ultrasound. In the control group, the full clinical effect of treatment was observed only in 10 patients (52.6%). 9 women (47,4%) required repeated therapy. Ultrasound studies in 12 patients (63.2%) showed changes equivalent to endometritis.

scholarly journals Improving of treatment of periodontal diseases in workers of the woodworking industry of the Chernivtsi region

2021 ◽  
Vol 106 (2) ◽  
pp. 28-35
Author(s):  
V. Batig ◽  
◽  
O. Tokar ◽  
I. Burdenyuk ◽  
◽  
...  
Keyword(s):  
Maintenance Therapy ◽  
Comparison Group ◽  
Periodontal Diseases ◽  
Main Group ◽  
Control Group ◽  
Treatment And Prevention ◽  
Group A ◽  
Group B ◽  
Periodontal Indices

Goal. Improving the scheme of treatment and prevention of periodontal diseases in workers of the woodworking industry of Chernivtsi region, who in the process of professional activity have long-term contact with unfavorable factors of the production environment. Materials and methods. 70 workers of the woodworking industry with diagnosed chronic generalized periodontitis of the I degree were treated (35 people – the main group (A); 35 people – comparison group (B)), age – from 25 to 45 years. To compare the results of laboratory studies, an additional survey of 25 healthy individuals of the same age with an intact periodontium were conducted. They formed the control group (C). Clinical examination of patients was performed according to standart methods: subjective (complaints, medical history, life history) and objective (examination, periodontal indices, determination of the level of gingival attachment). Laboratory methods of research included determination of urease and lysozyme activity in saliva, degree of dysbiosis of oral cavity. As maintenance therapy, patients of the main group were prescribed the proposed composition DEPE. Results. After the treatment, a significant improvement in the hygienic status of patients in both groups was observed, but difference between groups A and B in the indicators of oral hygiene after treatment was no statistically significant (pA2–B2 > 0.05). There was a significant improvement in periodontal indices after treatment in patients of both groups (PMA index according to C. Parma, bleeding index according to Muhlemann in Cowell I. modification, Russell periodontal index, PSR-test), but the indicators in patients of the main group were significantly better – pA2–B2 < 0.05. In patients of the main group (A) the level of attachment loss decreased by 1.83 times, comparison group (B) – by 1.71 times. The difference in levels of attachment loss after treatment between groups A and B is statistically significant (pA2–B2 < 0.05). The activity of the enzyme urease in patients of the main group (A) and comparison group (B) after treatment was significantly reduced (pA1–A2 < 0.001, pB1–B2 < 0.001), but only in the main group it reached the level in the control group (C) (pA2–C > 0.05). The level of lysozyme after treatment was significantly increased in patients of both observation groups (pA1–A2 < 0.001, pB1–B2 < 0.001), and reached the level of lysozyme in patients of the control group (C) (pA2–C > 0.05, pB2–C > 0.05). The degree of oral dysbiosis in patients of the main group (A) was significantly reduced by 5.43 times (pA1–A2 < 0.001), and its difference from the degree of dysbiosis in patients of the control group (C) is statistically insignificant (pA2–C > 0.05 ). The degree of oral dysbiosis in patients of the comparison group (B) was significantly reduced by 3.04 times (pB1–B2 < 0.001), but still remained significantly different from oral dysbiosis in patients of the control group (C) (pB2–C < 0.05). Conclusion. The proposed pharmacological composition DEPE is an effective antiseptic solution and can be used in the phase of maintenance therapy to improve the treatment and prevention of periodontal disease in workers of woodworking industry. Key words: decamethoxine, propolis, ethonium, generalized periodontitis, woodworking industry.

scholarly journals Вплив лікувально-профілактичних заходів на показники ротової рідини та клінічний стан тканин пародонта у дітей 6–7 років, хворих на гінгівіт

2017 ◽  
Author(s):  
O. V. Avdeev ◽  
Y. K. Zmarko ◽  
A. B. Boykiv ◽  
R. O. Drevnitska
Keyword(s):  
Clinical Supervision ◽  
Oral Fluid ◽  
Urease Activity ◽  
Comparison Group ◽  
Local Therapy ◽  
Main Group ◽  
Control Group ◽  
Results Of Treatment ◽  
Comparative Group ◽  
Anti Inflammatory

The high prevalence of inflammatory processes in the periodontitis of children, the ineffective effectiveness of preventive and curative measures can contribute to the development of generalized periodontitis, therefore it remains urgent to develop pathogenetic effects in the treatment of chronic catarrhal gingivitis.The aim of the study – a comparative assessment of the traditional treatment of chronic catarrhal gingivitis in children aged 6–7 years with therapy using an anti-inflammatory gel with neovitin.Materials and Methods. 61 children aged 6–7 years, suffering from chronic catarrhal gingivitis, were taken under clinical supervision and divided into groups: the main (30 children) and the comparative (31 children). All children underwent conventional therapy in accordance with the protocols for the provision of medical care – basic therapy. In the main group, for a month, pathogenetic agents were used: an anti-inflammatory gel with neovitin, hygiene products were recommended. In the comparative group, 3 % hydrogen peroxide solution was used for local therapy, for rinsing of the mouth (during the first week of treatment) – Rotokan, a therapeutic and prophylactic toothpaste.Examination and control examinations were carried out with the hygienic state of the mouth determined by the hygienic index of Yu. A. Fedorov and V. V. Volodkina, prevalence and intensity of the inflammatory process in the gums with the help of the Schiller-Pisarev test and the PMA index, oral fluid index (lysozyme content, formation of oxydradicals, urease activity, degree of dysbiosis, pH and viscosity).Results and Discussion. After the treatment, elimination of gum inflammation in patients of the main group was accompanied by a decrease in the PMA index by 3.8 times; formation of oxyradicals decreased in children of the main group by 6.35 %; in the comparative group – by 6.15 %. Urease activity decreased in children of the main group by 16.37 % (p<0.05), the viscosity of the oral fluid of children decreased in 2.65 times in the main group and in 2.13 times in the comparative group (p<0.05). The lysozyme content increased in children of the main group by 15.61 % (p<0.05) in the comparison group – by 9.63% (p<0.05). The treatment after 0.5 years caused an increase in the lysozyme content in the oral fluid of the children of the main group to the level of the control group. The degree of dysbiosis decreased in the main group, in the comparison group tended to increase.Conclusions. The use of the proposed therapy with gel with neovitin contributed to the best results of treatment: reducing the degree of inflammation of the gums, the number of visits to the doctor, improving the indices of nonspecific protection in the oral fluid of 6-7 years-old children and, to a greater extent, six months after the treatment.

scholarly journals FEATURES OF CLINICAL MANIFESTATIONS, CELLULAR AND HUMORAL IMMUNITY OF NEWBORNS WITH INTRAUTERINE MIXED INFECTIONS

2020 ◽  
pp. 427-433
Author(s):  
M.A. YUSUPOVA ◽  
◽  
K.I. ISMOILOV ◽  
Keyword(s):  
Humoral Immunity ◽  
Phagocytic Activity ◽  
Clinical Manifestations ◽  
Main Group ◽  
Phagocytic Index ◽  
Control Group ◽  
Mixed Infections ◽  
Secondary Immunodeficiency ◽  
Group I ◽  
Cellular And Humoral Immunity

Objective: To study the features of clinical manifestations, cellular and humoral immunity of newborns with intrauterine mixed infections (IUMI). Methods: A comprehensive survey of 45 infants with IUMI was carried out. Depending on the classification of IUMI they were divided into 3 main groups: group I – 24 patients (53%) with the viral-bacterial association, group II – 12 patients (27%) with the viral-viral association, and group III – 9 patients (20%) with the viral-parasitic association. The control group consisted of 10 newborns born from uninfected, somatically healthy mothers. Diagnosis of IUMI was based on the detection of specific antibodies of the IgA, IgG and IgM classes, as well as phagocytic activity and phagocytic index of leukocytes. In addition, general clinical, biochemical, bacteriological and instrumental research methods have been conducted. Results: The blood serum IgA and IgM indices in patients of the main group were significantly higher, and the mean IgG values were lower compared to the control group. Analysis of cellular immunity parameters in the main group showed a decrease in the number of mature T-lymphocytes (CD3), B-lymphocytes (CD20), the number of T-helpers and cells that produce IL-2 in the peripheral blood, compared with the control group. In the main group, there was also an increase in the number of apoptosis cells (CD95), cells with high cytotoxic activity (CD25, CD71) and the percentage of natural killer cells (CD16). A decrease in the phagocytic activity and phagocytic index of neutrophils was recorded, which indicates the insufficiency of the nonspecific component of immunity. Conclusions: In newborns, various changes were found both on the part of specific and nonspecific components of immunity. This indicates the development of secondary immunodeficiency in this category of patients and makes it necessary to add to the main treatment of immune corrective therapy. Keywords: Congenital infections, mixed intrauterine infections, TORCH syndrome, opportunistic infections, secondary immunodeficiency.

scholarly journals Standard open operative interventions in surgical treatment of patients, suffering an acute complicated pancreatitis

2018 ◽  
Vol 85 (7) ◽  
pp. 27-29
Author(s):  
V. P. Аndriushchenko ◽  
D. V. Аndriushchenko ◽  
Yu. S. Lysiuk
Keyword(s):  
Surgical Treatment ◽  
Comparison Group ◽  
Surgical Intervention ◽  
Main Group ◽  
Control Group ◽  
Treatment Results ◽  
Surgical Interventions ◽  
Second Stage ◽  
Standard Operations

Objective. To determine the character and optimal volume of standard open surgical interventions in an acute complicated pancreatitis (ACP) with elaboration of the main technical elements of the operations. Маterials and methods. There were operated 96 patients, suffering ACP. The main group consisted of 47 patients, in whom open interventions were performed primarily in 19, and as the second-stage procedure after application of miniinvasive interventional technologies (МIТ) – in 28. The comparison group consisted of 49 patients, in whom standard operations were done only. Results. Application of MIТ have promoted the indications narrowing for performance of primary open operations more than twice. In the main group the arcuate-like subcostal access was applied predominantly - in 26 (55%) observations (χ²=14.287; р=002), while in a control one – a median upper laparotomy – in 37 (76%) observations (χ²=38.43, р < 0.001). The method of closed draining in accordance to procedure of Beger was used predominantly in the main group of patients – in 43% of observations, comparing with a control group - 9% of observations (χ²=12.965; р=0.003). In 23% patients of the main group and in 26% patients of a control one (χ²=0.0013; р=0.05), when the extended purulent-necrotic inflammation process have presented, a staged sanation was applied, using programmed relaparotomies. Some technical elements of the surgical intervention were improved. Conclusion. Application of standard open operations in accordance to elaborated principle is accompanied by improvement of the treatment results in patients, suffering ACP.

scholarly journals Content of blood serum bone tissue metabolism indices in patients with prostheses instability after primary knee joint arthroplasty

2019 ◽  
Vol 36 (3) ◽  
pp. 5-10
Author(s):  
Elena A. Galashina ◽  
Vladimir Yu. Ulyanov ◽  
Aleksandr S. Bondarenko
Keyword(s):  
Vitamin D ◽  
Knee Joint ◽  
Blood Serum ◽  
Bone Tissue ◽  
Comparison Group ◽  
Joint Arthroplasty ◽  
Main Group ◽  
Tissue Metabolism ◽  
25 Oh Vitamin D ◽  
Observation Period

Aim. To assess the separate indices of blood serum bone tissue metabolism content in patients with instability of prostheses after the primary knee joint arthroplasty. Materials and methods. The main group joined 40 patients with implant-associated inflammation, the comparison group – 40 patients with primary aseptic instability of prostheses, the control – 20 conditionally healthy donors. The blood serum hPTH and 25-OH Vitamin D content was determined using solid-phase immune-enzyme assay. Results. Among the patients of the main group, blood serum hPTH concentration increased as compared with the control after1 and 12 months following the surgery and compared with each previous observation period; 25-OH Vitamin D level decreased compared with the control before the surgery, after1 and 12 months following the surgical intervention and compared with each previous period of observation. In the patients of the comparison group versus the control values, blood serum hPTH concentration elevated and 25-OH Vitamin D level declined after 1 and 12 months following the surgery and compared with the previous observation period 1 month later. Conclusions. When estimating the blood serum hPTH and 25-OH Vitamin D content in patients with instability of prostheses after the primary knee joint arthroplasty, there were revealed multidirectional changes in their levels, showing bone tissue metabolic disorders.

Markers of a metastatic niche in the omentum in ovarian cancer.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e18081-e18081
Author(s):  
Tatiana I. Moiseenko ◽  
Elena M. Frantsiyants ◽  
Irina V. Kaplieva ◽  
Valeria A. Bandovkina ◽  
Natalia D. Cheryarina ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Growth Factors ◽  
Tumor Cells ◽  
Comparison Group ◽  
Metastatic Tumor ◽  
Main Group ◽  
Control Group ◽  
Metastatic Niche ◽  
Primary Tumors ◽  
Igf I

e18081 Background: Ovarian cancer spreads intraperitoneally due to the interaction of tumor cells and the omental mesothelium, creating a metastatic niche and supporting elements of cancer cell expansion such as adhesion, proliferation, migration, and neoangiogenesis. The purpose of the study was to analyze levels of VEGFA, IGF-I, IGF-II and TGFβ1 in omental tissues in ovarian cancer (OC). Methods: The main group included 23 patients with metastatic OC T3-4аN0-3M1; the comparison group – 21 patients with non-metastatic OC T3-4аN0-3M0; the control group – 19 non-cancer patients. Levels of VEGFA, IGF-I, IGF-II and TGFβ1 were measured by standard ELISA methods in tissues of primary tumors and the omentum. Results: Levels of growth factors in the comparison group were not elevated compared to control values. Growth factors in omental tissues in the main group were increased compared to control values: VEGFA – by 2.5 times, IGF-I – by 3.4 times, IGF-II – by 2.5 times, TGFβ1 - by 3.1 times. In the comparison group, the levels in omental tissues were lower than in the main group: VEGFA – by 1.7 times (p < 0.05), IGF-I – by 2.1 times, IGF-II – by 1.6 times (p < 0.05); TGFβ1 did not differ from the levels in the main group. Conclusions: Higher expression of VEGFА in the omentum with metastases can be considered a significant factor in the formation of signaling pathways between metastatic tumor cells and local non-cancer cells. IGF-I and IGF-II trigger the endothelial growth factor. The TGFβ1 activation in the omentum in metastatic ovarian cancer is necessary for the paracrine induction and transition of disseminated tumor and/or stem cells from the "sleeping" to the active state.

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