Isolasi dan Uji Aktivitas Antioksidan Ekstrak Metanol  Dari Buah Senduduk Bulu (Clidemia hirta (L.) D. Don)

A study on isolation and antioxidant activity of secondary metabolit of methanol extract of fruit of Senduduk Bulu (Clidemia hirta (L.) D. Don) has been carried out. Senduduk bulu is used for folk medicine to many diseases. Flavonoid and phenolic has been reported to its activity as antioxidant. The aim of this research was to find antioxidant compound and antioxidant activity of this fruit. Isolation works were conducted using Vacuum Liquid Chromatography and Column Chromatography method. Antioxidant activity was determined using DPPH free radical scavenging methods. The isolation works afforded 10 mg of pure compound CHP1 which was obtained as white crystal, with its melting point of 165–167ºC, soluble in chloroform and slightly soluble in methanol. This compound gave positive reaction with Liebermann-Burchard (LB) reagent and concluded as terpenoid. Based on it UV spectrum, this compound showed a λ max of 316 nm. Its FT-IR spectrum showed appearence of some functional groups of OH, C-H aliphatic, C=O ester,C=C alkene, CH bending and C-O. The 1H-NMR spectrum showed the alkene protons and methyl protons which is a characteristic of terpenoid compounds. The pure compound (CHP1) provided a weak antioxidant activity with the IC50 of 327,01µg/mL.

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Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

Journal of Chemistry ◽

10.1155/2013/192039 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 16

Author(s):

G. Kiran ◽

T. Maneshwar ◽

Y. Rajeshwar ◽

M. Sarangapani

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging ◽

Microwave Assisted Synthesis ◽

Stable Free Radical ◽

Ft Ir ◽

Antimicrobial And Antioxidant Activity ◽

New Compounds ◽

Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

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Phytochemical analysis of herbal teas containing caffeic acid

Romanian Journal of Pharmaceutical Practice ◽

10.37897/rjphp.2021.2.3 ◽

2021 ◽

Vol 57 (2) ◽

pp. 76-83

Author(s):

Mihai TODORAN ◽

◽

Liana HANGA ◽

Daniela BENEDEC ◽

◽

...

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Caffeic Acid ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Content ◽

Phytochemical Analysis ◽

Chromatography Method ◽

Caffeic Acid Derivatives ◽

Level I

Objectives. The present analysis was to investigate the content of caffeic acid derivatives, antioxidant activity, and the presence of caffeic acid in alcoholic extracts obtained from 16 simple teas from the following medicinal plants: Vaccinium mуrtilluѕ, Camеllia ѕinеnѕiѕ, Coffea arabica, Mеlіssa οffіcіnalіs, Οcіmum basіlіcum, Rοsmarіnus οffіcіnalіs, Salvіa οffіcіnalіs, Ηyssοрus οffіcіnalіs, Αrtеmіsіa absіntһіum, Cynara scοlymus, Calendula officinalis, Cοrіandrum satіvum, Fοеnіculum vulgarе, Carum carvі, Rοsa canіna, Crataеgus mοnοgyna. Materials and methods. The presence of caffeic acid was evaluated using the thin layer chromatography method (TLC). The total content of phenylpropanoids from the medicinal herbs alcoholic extracts was determined using a spectrophotometric method. Outcomes. Results were in the range of 0.046-2.426% caffeic acid derivatives (CAE%). The antioxidant properties were measured using the DPPH radical scavenging mechanism. The results were found to be in the range of 63.93% and 0.38% inhibition level (I%). The greatest antioxidant activity was measured for Camellia sinensis (I = 45.54%), followed by Vaccinium myrtillus (I = 50.289%). Conclusions. In this paper it was shown that these medicinal plants are rich in caffeic acid derivatives with antioxidant action capitalized in the prevention of serious diseases.

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Antioxidant Activity and Chemical Composition of Helichrysum lacteum Coss. & Dur.

The Natural Products Journal ◽

10.2174/2210315509666190603083300 ◽

2020 ◽

Vol 10 (4) ◽

pp. 429-439

Author(s):

Souad Arrif ◽

Mohammed Benkhaled ◽

Hamada Haba ◽

Soumia Mouffouk

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Property ◽

Pure Compound ◽

Etoac Extract ◽

Total Antioxidant ◽

Dpph Scavenging

Objective: : The objective of the present study was to measure antioxidant activity and to evaluate the phytochemical constitution of Helichrysum lacteum growing in Algeria. Methods: : The structure elucidation of the isolated compounds was performed by spectroscopic methods. Antioxidant activity of the extracts the crude extracts (PE, EtOAc, and n-BuOH) and pure compound (noryangonin (3)) was investigated by 5 methods, including DPPH free radical scavenging activity, reducing power and ferric thiocyanate methods, hydrogen peroxide scavenging capacity, and total antioxidant activity by phosphomolybdate assay. Results: : The chemical investigation of the EtOAc extract of the aerial parts of H. lacteum led to the isolation of two flavonoids, astragalin (1) and isoquercitrin (2), together with a styryl pyrone, noryangonin (3), and a cyclobutane dimer, achyrodimer C (4) as well as two triterpenoid compounds, β-sitosterol (5) and oleanolic acid (6). The highest phenolic and flavonoid contents were detected in the ethyl acetate extract. Noryangonin exhibited the highest antioxidant activity than those of the references and all the tested extracts, with a value of IC50 at 1.45±0.44 μg/mL in DPPH scavenging. In hydrogen peroxide scavenging essay, the results revealed that EtOAc extract exhibited the highest percent inhibition (63.15 %) as compared to the ascorbic acid (63.36 %) at the concentration of 100 μg/mL. In the phosphomolybdenum method, the EtOAc extract showed the strongest activity (36.85±0.02 μg EAA/mg ex) as compared to those of n-BuOH and PE extracts. Conclusion: : The antioxidant property shown in these findings needs further investigation, especially with the pure compound (3).

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Synthesis, characterization and antioxidant activity of 2-(4-Phenyl-6-p-Tolylpyrimidin-2-ylthio)-N-(4-substituted Phenyl)acetamides

Research Journal of Chemistry and Environment ◽

10.25303/2511rjce8692 ◽

2021 ◽

Vol 25 (11) ◽

pp. 86-92

Author(s):

Sureshkumar K. Dhakhda ◽

Malay J. Bhatt ◽

Jyotindra J. Bhatt

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Catalytic Amount ◽

Equimolar Amount ◽

Spectroscopic Techniques ◽

Dpph Radical ◽

Antioxidant Assay ◽

Starting Compound ◽

Ft Ir ◽

Dpph Radical Scavenging

A series of 2-(4-phenyl-6-p-tolylpyrimidin-2-ylthio)-N- (4-substituted phenyl)acetamide(4a-o) has been synthesized. Compounds have been characterized and tested for their antioxidant activity. The titled compounds (4a-o) have been synthesized by the reaction between 4-phenyl-6-p-tolylpyrimidine-2-thiol (2) with 2-chloro-N-substituted phenylacetamide (3ao) refluxed in acetone using catalytic amount of K2CO3. Compound (2) has been synthesized from (E)-1-phenyl- 3-p-tolylprop-2-en-1-one (1) with equimolar amount thiourea refluxed in alcoholic KOH to get compound (2). Starting compound chalcone was synthesized by Claisen-Schmidt condensation. All synthesized compounds have been characterized using spectroscopic techniques such as FT-IR, 1H NMR, 13C NMR, Mass Spectrometry and elemental analysis. Antioxidant assay has been performed by using DPPH radical scavenging method. Compounds 4-h and 4-o have shown good antioxidant activity.

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On the Characterization and Correlation of Compositional, Antioxidant and Colour Profile of Common and Balsamic Vinegars

Antioxidants ◽

10.3390/antiox7100139 ◽

2018 ◽

Vol 7 (10) ◽

pp. 139 ◽

Cited By ~ 6

Author(s):

Vassilia Sinanoglou ◽

Panagiotis Zoumpoulakis ◽

Charalambos Fotakis ◽

Nick Kalogeropoulos ◽

Aikaterini Sakellari ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Total Phenolic ◽

Spectra Analysis ◽

Fermentation Products ◽

Ft Ir ◽

Red Grape

Commercially available common and balsamic vinegars were examined, using a combination of spectrophotometric, chromatographic, colorimetric and spectroscopic methods. Total phenolic content, antioxidant activity, radical scavenging capacity, phenolic profile, colour parameters, Fourier Transform Infrared (FT-IR) absorbance spectra and Nuclear Magnetic Resonance (1H NMR) spectra were comparatively studied. The main scope was the assessment of vinegar antioxidant and metabolic profiles and the identification of the most appropriate features influencing their type and subtypes. Red grape balsamic vinegars exhibited the strongest antioxidant profile. High total phenolic content and radical scavenging-antioxidant activity of vinegars was strongly correlated with high hue-angle and colour density values and low lightness and a* values. FT-IR spectra analysis confirmed the presence of organic acids and carbohydrates and, in combination with Gas Chromatography-Mass Spectrometry (GC-MS), the occurrence of phenolic compounds. NMR spectroscopy enabled the identification of 27 characteristic metabolites in each type of vinegar. The combination of all applied techniques provides critical information on compositional differences among the vinegars and could serve as an application tool for similar fermentation products.

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Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells

Molecules ◽

10.3390/molecules26020416 ◽

2021 ◽

Vol 26 (2) ◽

pp. 416

Author(s):

Fabiana Giglio ◽

Maria Antonietta Castiglione Morelli ◽

Ilenia Matera ◽

Chiara Sinisgalli ◽

Rocco Rossano ◽

...

Keyword(s):

Antioxidant Activity ◽

Hepg2 Cells ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Redox Homeostasis ◽

Folk Medicine ◽

Radical Scavenging ◽

In Vitro Assays ◽

South Italy

Muscari comosum L. bulbs are commonly used as food in South Italy and also in folk medicine. By evaluating in vitro antioxidant activity and biological activities of their aqueous and methanol extracts, we shed light on the potential role, including both the nutraceutical and health benefits, of this plant. Total polyphenol content (TPC) and total flavonoid content (TFC) were evaluated by the Folin–Ciocalteu method and by the aluminum chloride method, respectively. Antioxidant activity was investigated by three in vitro assays and relative antioxidant capacity index (RACI) was calculated to compare results obtained by different tests. The extracts were tested to evaluate their possible involvement in redox homeostasis, using the human hepatoma (HepG2) cell line used as model. The extracts exhibited concentration/solvent dependent radical scavenging activity, as well as dysregulation of some genes involved in redox pathways by promoting Nrf2, SOD-2, GPX1, ABCC6 and ABCG2 expression. NMR metabolomics analysis suggests that HepG2 cells treated with Muscari comosum extracts experience changes in some metabolites involved in various metabolic pathways.

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Synthesis, Characterization, and Antioxidant Evaluation of Novel Pyridylurea-Functionalized Chitosan Derivatives

Polymers ◽

10.3390/polym11060951 ◽

2019 ◽

Vol 11 (6) ◽

pp. 951 ◽

Cited By ~ 4

Author(s):

Jingjing Zhang ◽

Wenqiang Tan ◽

Lijie Wei ◽

Fang Dong ◽

Qing Li ◽

...

Keyword(s):

Antioxidant Activity ◽

Structural Characteristics ◽

Radical Scavenging ◽

Cytotoxicity Test ◽

Chitosan Derivatives ◽

Ester Exchange ◽

1H Nuclear Magnetic Resonance ◽

Ft Ir ◽

Ester Exchange Reaction

In order to improve the bioactivity of chitosan, we synthesized a novel series of chitosan derivatives: firstly, chitosan was reacted with methylclhlorofonmate obtaining N-methoxyformylated chitosan (1), which was then converted into N-pyridylurea chitosan derivatives (2a-2c) by amine-ester exchange reaction. In addition, N-pyridylurea chitosan derivatives were conducted by reacting with iodomethane to obtain quaternized N-pyridylurea chitosan derivatives (3a-3c). The structural characteristics of as-prepared chitosan derivatives were confirmed by fourier transform infrared (FT-IR), 1H nuclear magnetic resonance (1H NMR), elemental analysis, and scanning electron microscope (SEM). Meanwhile, the antioxidant activity of the chitosan derivatives was assessed in vitro. As shown in this paper, the antioxidant activity decreased in the order: c > b > a. Moreover, after the quaternization with iodomethane, quaternized N-pyridylurea chitosan derivatives immediately exhibited enhanced antioxidant capacity compared with N-pyridylurea chitosan derivatives. For example, in 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, the scavenging activities of 3a-3c were 91.75%, 93.63%, and 97.63% while 2a-2c were 42.32%, 42.97%, and 43.07% at 0.4 mg/mL. L929 cells were also adopted for cytotoxicity test of chitosan and synthesized derivatives by CCK-8 assay and all samples showed decreased cytotoxicity. These results suggested that the novel pyridylurea-functionalized chitosan derivatives could be an ideal biomaterial.

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Synthesis, spectroscopic characterization, DFT, oxygen binding, antioxidant activity on Fe(III), Co(II) and Ni(II) complexes with a tetradentate ONNO donor Schiff base ligand

Journal of the Serbian Chemical Society ◽

10.2298/jsc201230037s ◽

2021 ◽

pp. 37-37

Author(s):

Satyendra Shukla ◽

Pratiksha Gaur ◽

Mohan Raidas ◽

Sanjay Bagri

Keyword(s):

Antioxidant Activity ◽

Schiff Base ◽

Schiff Base Ligand ◽

Density Functional ◽

Radical Scavenging ◽

Spectroscopic Characterization ◽

Binding Activity ◽

Oxygen Binding ◽

Ft Ir ◽

Analytical Tools

The Schiff base ligand, namely (7E)-N-benzylidene-2-styrylbenzena-mine1,2-diamine-2,4-dihydroxy-phenol, (L), was synthesized from condensation of 2,4-dihydroxybenzaldehyde with o-phenylenediamine. The reaction of the ligand with Fe(III), Co(II) and Ni(II) salts in a 1: 1 ratio yielded three complexes 1-3. Different analytical tools like elemental analysis, ESI-mass spectra, FT-IR, conductivity, electronic spectra, magnetic susceptibility, NMR and EPR mea-surements are used to elucidate the structure of the ligand and complexes. Density Functional Theory (DFT) calculation at the B3LYP/3-211G++/LANL2DZ level of the theory has been carried out to optimize the geometry of the ligand and complexes. The tetradentate ligand has coordinated to metals through ONNO donors affording octahedral geometry. Complexes were studied for their oxygen-binding activity and free radical scavenging activities. Complex 1 and 2, which contain Fe(IIl) and Co(II), displayed reversible oxygen binding activity. On the other hand, complex 3 fails to show oxygen binding. The order of antioxidant activity is 3 > 1 > 2 > L.

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Biological Value of Centaurea damascena: Minireview

Journal of Basic and Applied Research in Biomedicine ◽

10.51152/jbarbiomed.v5i2.41 ◽

2019 ◽

Vol 5 (2) ◽

pp. 99-106

Author(s):

Mohammad Jaafreh ◽

Haitham Qaralleh ◽

Muhamad Al-limoun

Keyword(s):

Antioxidant Activity ◽

Folk Medicine ◽

Radical Scavenging ◽

Dry Weight ◽

Antibacterial Activities ◽

Essential Oil Composition ◽

Total Phenolic ◽

Oil Composition ◽

Phenolic Contents ◽

The Family

The family Asteraceae include large number of Centaurea species which have been applied in folk medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial activity. The aim of the study was to discuss antibacterial activities of essential oil composition and methanolic extract of the same plant aerial part leaves. Thirty-seven components were characterized with 86% of oxygenated terpenes. The composition was dominated by 11.45% Fokienol, 8.8% thymol, 8.21% Alpha Terpineol, 7.24% Chrysanthemumic acid, 7.13% Terpinen-4-ol and 6.59% Borneol with a high degree of polymorphism in the occurrence of these compounds as compared with the different species of centaurea.. Free radical scavenging capacity of the C. damascna methanol extract was calculated by DPPH and FRAP test. DPPH radicals were scavenged with an IC50 value of 17.08 µg /ml. Antioxidant capacities obtained by the FRAP was 51.9 and expressed in mg Trolox g-1 dry weight. The total phenolic compounds of the methanol extracts of aerial parts, as estimated by Folin–Ciocalteu reagent method, was about 460 mg GAE/ g. The phenolic contents in the extracts highly correlate with their antioxidant activity, (R2 = 0.976) confirming that the antioxidant activity of this plant extracts is considerably phenolic contents-dependent.

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Chemical Properties, Fatty-Acid Composition, and Antioxidant Activity of Goji Berry (Lycium barbarum L. and Lycium chinense Mill.) Fruits

Antioxidants ◽

10.3390/antiox8030060 ◽

2019 ◽

Vol 8 (3) ◽

pp. 60 ◽

Cited By ~ 11

Author(s):

Prodromos Skenderidis ◽

Dimitrios Lampakis ◽

Ioannis Giavasis ◽

Stefanos Leontopoulos ◽

Konstantinos Petrotos ◽

...

Keyword(s):

Antioxidant Activity ◽

Fatty Acid ◽

Radical Scavenging ◽

Attenuated Total Reflection ◽

Lycium Chinense ◽

Total Carbohydrate ◽

Lycium Barbarum ◽

Goji Berry ◽

Ft Ir

In this study, the content composition and antioxidant activity of goji berry fruits from two species (Lycium barbarum and Lycium chinense) were assessed. The total carbohydrate and phenolic contents were evaluated using attenuated total reflection Fourier-transform infrared (ATR-FT-IR) spectroscopy, while the antioxidant activity of fruits was examined with two in vitro methods, which are based on the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2’-azino-bis(3-ethyl-benzthiazoline-sulfonic acid) (ABTS•+) free radicals. The fatty-acid profile was determined using gas chromatography coupled with mass spectrometry (GC-MS). The results of this study indicate that the fruits of L. barbarum present higher concentrations in carbohydrates and phenolics than L. chinense Mill. fruits. Furthermore, the antioxidant activity based on the half maximal inhibitory concentration (IC50) measurements of DPPH• and ABTS•+ free-radical scavenging was higher in L. barbarum than L. chinense Mill. Also, the GCMS analysis confirms the high levels of linoleic, palmitic, and oleic acids contained in the fruits of both species. Finally, the results of this study clearly show that the concentration of bioactive and antioxidant molecules is higher in L. barbarum than in L. chinense fruits, which was also confirmed by ATR-FT-IR measurements.

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