scholarly journals Induction of Rhizoctonia resistance to odontoglossum ringspot virus (ORSV) infection on orchid leaf organ viability

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Eka Nuraini Tohari ◽  
Mahfut Mahfut ◽  
Tundjung Tripeni Handayani ◽  
Sri Wahyuningsih
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Viral Infections ◽  
Economic Value ◽  
Ornamental Plants ◽  
Natural Sciences ◽  
Leaf Length ◽  
The Public ◽  
Length Width ◽  
Number Of Leaves

Orchid plants are in great demand by the public as ornamental plants that have economic value and the beauty of attractive flower shapes and colors, especially Phalaenopsis and Dendrobium. Related to this, conservation efforts are needed through cultivation. However, these efforts are often hampered by viral infections. One of the viruses that trigger inhibition of orchid cultivation that often appears is ORSV. Control efforts that are relatively safe and efficient can utilize mycorrhizae in orchids which play a role in increasing the absorption of plant nutrients. The use of Phalaenopsis and Dendrobium with the induction of Rhizoctonia sp. against ORSV is a very important alternative biofertilizer and biocontrol agent. The objectives of this study were 1) to determine the effectiveness of orchids induced by Rhizoctonia resistance, 2) to determine the viability of orchid leaf organs to ORSV infection, 3) to determine the viability of orchid root organs resulting from Rhizoctonia resistance to ORSV infection. This study was conducted in January 2021 – March 2021 at the Botanical 2 Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This study employed a completely randomized factorial with 6 treatments and 4 replications. The variable observed in this study was the effectiveness of mycorrhizae, leaf and root length, number of leaves and roots, and leaf width. The data obtained were homogenized using Levene's test and then analyzed by ANOVA and Tukey's further test at the 5% level. The results showed that the effectiveness of mycorrhizae occurred on the 3rd day of incubation. In leaf organs, Phalaenopsis amabilis showed an increased response and Dendrobium discolour showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.  

scholarly journals Foliar application of micronutrients enhances growth, flowering, minerals absorption and postharvest life of tuberose (Polianthes tuberosa L.) in calcareous soil

2021 ◽  
pp. 41-47
Author(s):  
Sana Mudassir ◽  
Riaz Ahmad ◽  
Muhammad Akbar Anjum
Keyword(s):  
Root Length ◽  
Calcareous Soil ◽  
Foliar Application ◽  
Calcareous Soils ◽  
Leaf Length ◽  
Spike Length ◽  
Vase Life ◽  
Flower Initiation ◽  
Zn Concentration ◽  
Number Of Leaves

Micronutrients applications are effective for better crop production in calcareous soils because these soils are usually deficit in iron (Fe), zinc (Zn) and boron (B). In Pakistan, most of soils are calcareous in nature. When tuberose is grown in such soils as a cut flower, its production is negatively affected. Therefore, a study was aimed to evaluate the effects of micronutrients as foliar sprays on cut tuberose production in calcareous soil. Micronutrients (Fe, Zn and B) alone and in combinations were sprayed on the plants after 60, 90 and 120 days of planting. The mixture of all these three micronutrients increased the plant height (95.77 cm), chlorophyll content (38.13 SPAD), number of leaves (79.63), leaf length (44.73 cm), fresh (111.64 g) and dry (16.16 g) plant weights, root length (15.13 cm), number of stalks (3.73), stalk length (79.03 cm), spike length (22 cm), number of florets (51.67), floret fresh weight (11.85 g), leaf Zn concentration (53.6 mg/g) and vase life (8.4 days). Fe + Zn enhanced the number of leaves (76.60), leaf length (45.83 cm), root length (15.05 cm), spike length (22.33 cm), and leaf Fe (128.18 mg/g) and Zn concentrations (55.02 mg/g). The Fe spray increased the leaf length (44.10 cm), days to flower initiation (142.47 days) and leaf Fe concentration (130.75 mg/g) in tuberose plants. Application of Zn improved the leaf length (45.87 cm) and diameter (1.32 cm), root (15.03 cm), spike (21.77 cm) and floret lengths (4.74 cm), floret dry weight (1.49 g) and leaf Zn concentration (57.5 mg/g). Foliar spray of B increased the B concentration (21.1 mg/g) in tuberose leaves. It is concluded that foliar application of micronutrients alone and in combinations improved the plant growth, flowering, leaf minerals concentrations and vase life of tuberose spikes. However, mixture of Fe, Zn and B was more effective as compared to other treatments. Moreover, current study encourages the foliar application of micronutrients in tuberose when grown in calcareous soils.

scholarly journals The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

2017 ◽  
Vol 21 (2) ◽  
pp. 109 ◽  
Author(s):  
Aries Bagus Sasongko ◽  
Asruwaidah Fatumi ◽  
Ari Indrianto
Keyword(s):  
Carbon Dioxide ◽  
Root Length ◽  
Unit Area ◽  
Leaf Length ◽  
Growth Increase ◽  
Photoautotrophic Micropropagation ◽  
Average Quantity ◽  
Chlorophyll A And B ◽  
Number Of Leaves

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.

scholarly journals Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

2018 ◽  
Vol 4 (2) ◽  
pp. 93
Author(s):  
Douglas Bertoncelli ◽  
Guilherme Alves ◽  
Gustavo Freiria ◽  
Felipe Furlan ◽  
Helio Neto ◽  
...  
Keyword(s):  
Root Length ◽  
Iron Concentration ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Nutritional Requirement ◽  
Initial Development ◽  
Shoot Height ◽  
Shoot Number ◽  
Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

scholarly journals Selection of Peanut Skin as a Growing Medium for Moon Orchid (Phalaenopsis amabilis)

2020 ◽  
Vol 20 (2) ◽  
pp. 237
Author(s):  
Nur Rokhimah Hanik ◽  
Sri Harsono ◽  
Anwari Adi Nugroho
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Leaf Length ◽  
Peanut Shell ◽  
Advantages And Disadvantages ◽  
Peanut Skin ◽  
Significant Difference ◽  
Peanut Shells ◽  
Number Of Leaves ◽  
Peanut Skins

Ferns are a planting medium that is commonly used in Phalaenopsis cultivation, but the growing demand for fern will increase the selling price and the availability of fern is limited. So a solution is needed to reduce the maintenance and maintenance costs by utilizing peanut shells as a growth medium. The purpose of this study is: 1. To determine the effect of peanut skin media on the growth of Phalaenopsis amabilis month orchid 2. To find out better which influence between fern media, fern media plus peanut skins, or peanut skin media on orchid growth month of Phalaenopsis amabilis. When the research began in May 2019 until October 2019. The research object was in the form of a 4-5 month-old orchid, the research location in the Biology Laboratory of the Faculty of Veterinary Science, Bangun Nusantara University, Sukoharjo. Simple randomized study design with three treatments. Treatment A by giving fern media, treatment B by giving fern media mixed with peanut skin, and treatment C by giving peanut skin media. The research variables are the number of leaves, leaf length, leaf area, number of roots and root length measured once a week for 8 weeks. Data analysis with 2. Variance Test (Anova or Kruskal Wallis test) 3. Duncan Multiple Range Test (DMRT) or Moods Median Test. From the results of the study it was found that all observed growth variables (number of leaves, leaf width, leaf length, leaf area, number of roots, average root length, and wet weight) were treated with fern, mixed (Fern and peanut shell) media, and media of peanut skin, there was no significant difference in the effect on the growth of the month orchid. Which means that the three types of media both have an influence on the growth of Phalaenopsis amabilis month orchids. media ferns and peanut shells have their advantages and disadvantages of each. So it can be concluded that, 1. The use of peanut skin media can affect the growth of Phalaenopsis amabilis month orchids. 2. Media fern, a mixture of fern media and peanut skins, and peanut skin media can provide the same effect on the growth of Phalaenopsis amabilis month orchids. From the results of the study it can be recommended that peanut skins can be used as a medium or a mixture of growing media of Phalaenopsis amabilis orchids.

scholarly journals Effects of Nutrient Sources on the Early Growth of Pineapple Plantlets (Ananas comosus (L) Merr) in the Nursery

2012 ◽  
Vol 20 (2) ◽  
pp. 35-40
Author(s):  
Solomon Olusegun Omotoso ◽  
Ezekiel Akinkunmi Akinrinde
Keyword(s):  
Root Length ◽  
Poultry Manure ◽  
Early Growth ◽  
Commercial Production ◽  
Leaf Length ◽  
Ananas Comosus ◽  
Mineral Fertilization ◽  
Nutrient Sources ◽  
Inadequate Information ◽  
Number Of Leaves

Abstract Inadequate information on the nutrition of pineapple limits its commercial production in Nigeria. The effects of poultry manure and urea (each at 50 and 100 kg N ha-1), as well as their 1:1 (organo-mineral) combinations at 50, 100, 150 and 200 kg N ha-1, on the early (nursery) growth of pineapple plantlets. Organo-mineral fertilization at 100 kg N ha--1 resulted in the highest D-leaf length, number of leaves, root length and leaf area, while the highest biomass production (approx. 304 g, just within the 300-400 g desirable range for transplanting to the field) was achieved at 150 kg N ha-1.

scholarly journals Effects of Potassium Deficiency on Growth of Pakchoi in Cold and Dry Areas

2021 ◽  
Author(s):  
Shi-Heng LI ◽  
Wei-Ming LI ◽  
Ling-Hao BU
Keyword(s):  
Leaf Area ◽  
Root Length ◽  
Growth And Development ◽  
Leaf Length ◽  
Potassium Deficiency ◽  
Leaf Width ◽  
Growth Status ◽  
Dry Areas ◽  
Number Of Leaves

Abstract [Background] To reveal the effect of potassium deficiency on the growth and development of pakchoi in cold and dry areas.[Results] The effect of potassium deficiency on the growth status of P. pakchoi in cold and dry areas was significant. P. pakchoi treated with potassium-free nutrient solution had the best growth, the largest number of leaves, leaf length, leaf width, leaf area, root length and weight; P. pakchoi treated with 100% potassium deficiency had the smallest plant, leaf length, leaf width, leaf area, root length and weight; P. pakchoi treated with potassium deficiency 50% had the middle growth and development status, and the root ratio was the smallest in d 21.[Conclusion] Potassium can effectively promote the growth and development of pakchoi in cold and dry areas, increase its root length, leaf width, leaf area, weight, etc. The quality of the upper part and the underground part of d,21 of pakchoi treated with deficiency is the most different.

scholarly journals Bacillus Pumilus Induced Tolerance of Maize (Zea Mays L.) Against Cadmium (Cd) Stress

2021 ◽  
Author(s):  
Asim Shahzad ◽  
Mahmood Elahie ◽  
Muhammad Naeem ◽  
Tasmia Bashir ◽  
Humaira Yasmin ◽  
...  
Keyword(s):  
Root Length ◽  
Bacillus Pumilus ◽  
Germination Percentage ◽  
Leaf Length ◽  
Shoot Length ◽  
Maize Plant ◽  
Cd Stress ◽  
Fresh Weight ◽  
Number Of Leaves ◽  
Maize Plants

Abstract Purpose: Heavy metals contaminate the soil that alters the properties of soil and negatively affect plants growth. Using microorganism and plant can remove these pollutants from soil. The present investigation was designed to evaluate the induced effect of Bacillus pumilus on maize plant in Cadmium (Cd) contaminated soil. Methods: Three different concentrations of Cd (i.e. 0.25, 0.50 and 0.75 mg kg-1) were applied in soil under which maize plants were grown. The germination percentage, shoot length, leaf length, number of leaves, root length,fresh weight and nutrient uptake by maize plant were determined. The experiment was conducted by using complete randomized design (CRD) with three replicates. Results: The result indicated that germination percentage, Shoot length, leaf length, root length, number of leaves, and plant fresh weight were reduced by 37, 39, 39, 32 and 59% respectively at 0.75mg kg-1 of CdSO4concentration but when maize seeds inoculated with Bacillus pumilus significantly increased the germination percentage, shoot length, leaf length, number of leaves, plant fresh weight at different concentrations of CdSO4. Moreover, the plant protein were significantly increased by 60% in T6 (0.25 mg kg-1 of CdSO4+inoculated seed) and Peroxidase dismutase (POD) was also significantly higher by 346% in T6 (0.25 mg kg-1 of CdSO4+inoculated seed), however, the Superoxide dismutase (SOD) was significantly higher in T5 (0.75 mg kg-1 of CdSO4+ uninoculated seed) and was 769% higher as compared to control. The Cd contents in Bacillus pumilus inoculated maize roots and shoots were decreased. Conclusion: The present investigations indicated that the inoculation of maize plant with Bacillus pumilus can help maize plants to withstand Cd stress but higher concentration of Cd can harm the plant. The Bacillus pumilus has good potential to remediate Cd from soil, and also have potential to reduce the phytoavailibility and toxicity of Cd.

scholarly journals PERTUMBUHAN PLANLET KRISAN (Dendranthema grandiflora Tzvelev) KULTIVAR PINK FIJI SETELAH PENAMBAHAN EKSTRAK TAUGE (Vigna radiata L.) PADA MEDIUM MURASHIGE DANSKOOG (MS) SECARA IN VITRO.

2019 ◽  
Vol 5 (2) ◽  
pp. 36-41
Author(s):  
Nalindri Impitasari ◽  
Endang Nurcahyani ◽  
Tundjung Tripeni Handayani ◽  
Yulianty Yulianty
Keyword(s):  
Vigna Radiata ◽  
Economic Value ◽  
Ornamental Plants ◽  
Natural Sciences ◽  
Ms Medium ◽  
Dendranthema Grandiflora ◽  
Community Interest ◽  
Randomized Design ◽  
Completely Randomized Design

Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the important ornamental plants in Indonesia and has high economic value . This plant is known as a producer of flowers with attractive shapes and colors . Seeing the magnitude of community interest and the potential utilization of chrysanthemum , causing this plant more and more developed and cultivated . This study aims to determine the concentration of optimum mungbean sprouts extract on the growth of chrysanthemum explants in vita . The addition of mungbean sprouts extracts (Vigna radiata L .) from concentration of 0% v/v , 2% v/v , 4% v/v , 6% v/v and 8% v/v on Murashige and Skoog(MS ) medium to growth eksplan Chrysanthemum (Dendranthema grandiflora Tzvelev ) Pink Fiji cultivars have been carried out in the tissue culture laboratory of Faculty of Mathematics and Natural Sciences , University of Lampung from November to December 2017 . This study used Completely Randomized Design (RAL ) 1 factor with 5 replications . Analysis of BNT variety and test is done at 5% level . The results showed that the extract from mungbean sprouts (Vigna radiata L . ) had no effect on plantlet height , number of shoot and number of chrysanthemum (Dendranthema grandiflora Tzvelev) plantlet leaves. The addition of mungbean spourts extracts on Murashige and skoog (MS) medium show 100% live plantlet.

scholarly journals UJI KONSENTRASI TIGA MACAM PUPUK ORGANIK CAIR DARI LIMBAH PERTANIAN TERHADAP PERTUMBUHAN DAN HASIL TANAMAN ARUGULA (ERUCA SATIVA)

2020 ◽  
Vol 22 (1) ◽  
pp. 36
Author(s):  
Sulistiawan Purnomo Aji ◽  
Siswadi Siswadi ◽  
Saiful Bahri
Keyword(s):  
Plant Height ◽  
Root Length ◽  
Growth Process ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Leaf Length ◽  
Leaf Width ◽  
Banana Peel ◽  
Eruca Sativa ◽  
Number Of Leaves

This study is entitled Concentration Test of Three Liquid Organic Fertilizer From Agricultural Waste on Growth and Yield of Arugula (Eruca Sativa) to test the concentration of three kinds of liquid organic fertilizer, sugarcane bagasse waste, banana peel and coconut fiber with a concentration of 50 ml/l, 100 ml/l, and, 150 ml/l. This study uses a single Randomized Complete Design (CRD) method consisting of 10 treatments that are repeated 3 times, consisting of K0, K1, K2, K3, K4, K5, K6, K7, K8, K9, the results of this study were analyzed with Duncan's Multiple Range Test (DMRT) at 5%, the parameters observed were: plant height, number of leaves, leaf width, leaf length, root length, fresh weightof biomass, and dry weight of biomass. The results of the study show that: (1) Giving treatment (K3), liquid organic fertilizer of banana peels with a concentration of 150 ml/l, provides interaction with parameters of observation of leaf width, root length and dry weight of biomass arugula plants, but they need to increase the concentration of organic fertilizer liquid to assist the growth process with observations of plant height parameters, number of leaves, leaf length and fresh weigh of biomass. (2) Provision of treatment with 150 ml/l concentration is the best treatment for the parameters of observation of leaf width, length of root and dry weight of biomass, but the administration of 150 ml/l concentration is not sufficient for plant nutrient requirements so there is no interaction with the growth process of plant height, number of leaves, length of leaves and fresh weight of biomass. (3) The highest dry weight of biomass in the treatment of liquid organic fertilizer from banana peel waste with a concentration of 150 ml/l, weighing 4.32 g or an increase of 20% compared to the control.

scholarly journals The Effect of Benzyl Amino Purine (BAP) Concentration on the Growth Amount of the Explant of Dendrobium spectabile Orchid by In-Vitro

2020 ◽  
Vol 3 (2) ◽  
pp. 33
Author(s):  
Ario Ario ◽  
Setiawan Setiawan
Keyword(s):  
Economic Value ◽  
Aloe Vera ◽  
Leaf Length ◽  
Plant Seeds ◽  
Randomized Design ◽  
In Vitro Technique ◽  
Number Of Leaves ◽  
Short Time ◽  
Benzyl Amino Purine

<em>Dendrobium</em><em> spectabile orchid is an ornamental plant of high economic value, having relatively durable properties, beauty, and attractiveness of orchids lie in the diverse shapes and colors of the flowers. Therefore, it requires rapid and efficient propagation techniques. One of them is in-vitro vegetative propagation. Plant propagation using the in-vitro technique has the opportunity to produce a large number of plant seeds in a relatively short time. This study aims to determine the correct concentration of Benzyl Amino Purine (BAP) on the growth amount of the explants of Dendrobium spectabile orchid by in-vitro. The research was conducted at the Biotechnology Laboratory of the Regional Technical Implementation Unit (UPTD) of the Aloe Vera Center (AVC) Pontianak. This research was conducted from January 1, 2020, to March 30, 2020. The method used was a Completely Randomized Design (CRD). The treatment consisted of 6 levels of treatment, namely b1 = 0.75 ppm, b2 = 1.00 ppm, b3 = 1.25 ppm, b4 = 1.50 ppm, b5 = 1.75 ppm, b6 = 2.00 ppm. All treatments were repeated 4 times for each treatment consisting of 3 plant samples. The research finding showed that the treatment of BAP concentration 1.50 was the best in increasing the average number of leaves 4.92 strands, the average number of shoots 4.25 buds, and the average leaf length was 2.44 cm.</em>

Sign in / Sign up

Export Citation Format

Share Document