scholarly journals Biofilm production as a virulence factor in Uropathogenic bacteria and yeasts

2013 ◽  
Vol 7 (1) ◽  
pp. 29-34
Author(s):  
Raghada S. Makia ◽  
Ayad M.A. Fadhil ◽  
Munera Ch. Ismail
Keyword(s):  
Test Tube ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Candida Spp ◽  
Isolation And Identification ◽  
Pseudomonas Spp ◽  
Biofilm Production ◽  
Tract Infections ◽  
Uropathogenic Bacteria

This study includes isolation and identification of different uropathogenes (bacteria- yeasts) collected from catheterized patients suffering from complicated urinary tract infections. Three hundred and fifty urine samples obtained by swabs from catheterized patients were identified for the presence of uropathogenes (bacteria and yeast). 221(63.13%) samples were obtained from females and 118 (33.71%) samples from male, 339(96.85%) sample were identified by culturing as a positive result, while 11(3.14%) sample were negative result. The 339 positive isolates include 303 (89.38%) bacterial isolates and 36 (10.61%) yeast isolates. Results of biochemical tests and Api 20 system for bacterial and yeast isolates reveals E.coli, Proteus spp, Klebseilla spp, Pseudomonas spp and Candida spp represented the main causative uropathogen infect urinary system and causing a complicated type of infection. The determination of bacterial and yeasts ability to form biofilm was carried out using test tube method , 306 isolates which represented (90.26%) were capable to form biofilm with differ in the thickness of formed layer. Pseudomonas spp formed the thicker biofilm followed by E.coli, Candida spp, Proteus spp, and Klebseilla spp.

Isolation and Identification of Fungal Isolates caused Otomycosis from some Hospitals and Clinics in Mosul with Determination of Their Virulence Factors

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Nabeel Al-Sharrad ◽  
Muhammad A. Al-Kataan ◽  
Maha A. Al-Rejaboo
Keyword(s):  
Fungal Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Fungal Pathogens ◽  
Candida Parapsilosis ◽  
Isolation And Identification ◽  
Fungal Isolates ◽  
Biofilm Production ◽  
Etiological Agents

Otomycosis is a fungal infection that frequently involves the external auditory canal. In this study, we aimed to isolation and identification the fungal isolates as etiological agents of otomycosis from some hospitals and clinics in Mosul with determination of their virulence factors of fungal etiological agents. Positive fungal infection was found in (43) samples (71.6%). The most common fungal pathogens were Candida and Aspergillus species, with Candida parapsilosis being the predominant isolates in (11) samples (16.6%). Otomycosis was more common in Female in (26) samples (43.3%).Otomycosis was the highest prevalence aged group 15-40 years (19) samples (31.3%). The present study of virulence factors revealed that the highest biofilm formation isolates were C. parapsilosis is (10) isolates which were distributed between (2) strong and (8) weak biofilm formation.Where C.trpicales, was recorded as least isolates for biofilm production.

scholarly journals Anti-Bacterial Effects of Essential Oils against Uropathogenic Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 358
Author(s):  
Maria Loose ◽  
Emmelie Pilger ◽  
Florian Wagenlehner
Keyword(s):  
Essential Oils ◽  
Additional Treatment ◽  
Cross Resistance ◽  
Synergistic Activity ◽  
Antibiotic Resistant ◽  
Tea Tree ◽  
Artificial Urine ◽  
Tract Infections ◽  
Uropathogenic Bacteria

Given the increasing antimicrobial resistance in urinary tract infections (UTI), alternative strategies need to be investigated. Determination of minimal inhibitory and bactericidal concentrations of essential oils from cajeput, lemongrass, tea tree, and thyme in artificial urine, revealed bactericidal activity of all four tested essential oils against seven uropathogenic species with values ranging between 0.78–50 mg/mL. Tea tree and thyme essential oils were more efficient than lemongrass and cajeput. In addition, antibiotic-resistant strains showed similar susceptibility as antibiotic-sensitive strains, suggesting no cross-resistance between antibiotics and these essential oils. Checkerboard assays revealed a synergistic activity of the combination of thyme and tea tree. Furthermore, the combination with thyme and tea tree essential oils increased the activity of fosfomycin and pivmecillinam, but not nitrofurantoin, against Escherichia coli. This study provides a basis for further investigation of the potential of thyme and tea tree oil as an alternative or additional treatment of UTI.

scholarly journals STUDY OF PREVALENCE OF URINARY TRACT INFECTIONS IN PATIENTS OF UJJAIN, MADHYA PRADESH (INDIA)

2020 ◽  
pp. 69-71
Author(s):  
Kaina Bhonsle ◽  
Harish Vyas ◽  
Kirti Hemwani ◽  
Alka Vyas
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Morphological Characteristics ◽  
Age Group ◽  
Bacterial Isolates ◽  
Gram Stain ◽  
Biochemical Tests ◽  
E Coli ◽  
Tract Infections ◽  
Rural Patients

Background: Urinary tract infections (UTI’s) define a condition in which urinary tract is infected with a pathogen causing inflammation. Urinary tract infections are one of the most prevalent infections affecting people of all age group from neonates to adults and are major cause of morbidity and several disorders in patients. In serious cases urinary tract infection lead to kidney failure, septicemia, bacterial endocarditis, prostatitis and infertility. Aim: The objective of this work was to the study the prevalence of urinary tract infections among patients of Ujjain and to identify prominent bacterial pathogens responsible for causing UTI. Methods: A total of 500 urine samples were collected aseptically and cultured on Blood agar and Mac’conkey agar. The bacterial isolates were identified based on their colony morphological characteristics, Gram stain reaction and their biochemical tests. Result: After overnight incubation a total of 307 out of 500 patients tested positive for UTI suggesting that (61%) of patients suffered from UTI infections. It was seen that (89%) of bacterial isolates causing UTI were Gram negative and (11%) were Gram positive. The identification of pathogens indicated that E. coli is most prevalent uropathogen followed by K. pneumonae, P. aeruginosa, E. faecalis, S. aureus and P.vulgaris. Conclusion: The study shows that prevalence of UTI is higher in females as compared to males. It was also been seen that UTI infections were prevalent in urban as well as in rural patients, however, the incidence of infection was slightly high in urban patients. This study is important as constant survey and identification of uropathogens is essential for effective treatment of UTI.

Isolation and identification of Candida species from urogenital tract infections of women in Baghdad city

2012 ◽  
Vol 36 (0E) ◽  
pp. 115-119
Author(s):  
Mohammed J. Alwan
Keyword(s):  
Pregnant Women ◽  
Candida Species ◽  
Urogenital Tract ◽  
Urine Samples ◽  
Candida Spp ◽  
Isolation And Identification ◽  
Urogenital Infection ◽  
Fungal Isolates ◽  
Vaginal Swabs ◽  
Tract Infections

In order to determine Candida Species isolates that associated with urogenital infection in women,174 vaginal swabs and 66 urine samples were collected from pregnant and non-pregnant women suffering from urogenital tract infection whom visted teaching Al-way hospital during the peroid from January to October 2011. The results showed that 32 out of 174 vaginal swabs were Candida Spp positive (18.3%), high percentage was reported in June and July (25%) for each one and no fungal isolates were recorded in March .The results also explained that high percentage of fungal isolates was recorded in pregnant women (68.7%) as comparing with non-pregnant women (31.2%) .Thirty –two fungal isolates were recorded which included : C.albicans(43.7%); C.tropicals(21.8%), C.prarapsilosis(12.5%) ;C.glabrata (9.3%);C. guilliermondii(6.2%); C. capitatum and C. krusei (3.1%) for each one . Also the study revealed that 9 out 66,(13.6%) of the urine samples were Candida spp positive which included : C.albicans was form high percentage (7.5%) followed by C.tropicals(4.5%) and C.glabrala (1.5%), these results indicated that albicans and non-albicans Candida may be associated with urogenital infection in women.

scholarly journals Detection of Antibiotics Sensitivity at Different PH Levels for Proteus Mirabilis Isolated from Patients with Urinary Tract Infections

2018 ◽  
Vol 26 (5) ◽  
pp. 132-144
Author(s):  
Ehsan F. Hussein
Keyword(s):  
High Activity ◽  
Proteus Mirabilis ◽  
Penicillin G ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Neutral Ph ◽  
Antibiotics Susceptibility ◽  
Antibiotics Sensitivity ◽  
Tract Infections

One hundred and sixty-nine samples of urine have been collected through the period from December/2016 to May/2017. Were for isolation and identification of Proteus mirabilis. Isolated from urine in patients with UTI. Identification was done by grow-  ing on different media and biochemical tests as well as the antibiotics susceptibility were studied by using twenty types of antibiotics at acidic pH, neutral pH and alkaline or basic pH by disc diffusion method. These antibiotics were involved Ciprofloxacin, Amikcin, Meromenem, Imipenem, Ampicillin, Rifampin, Gentamicin, Trimethoprim, Tetracycline, Amoxicillin, Sulfamethoxazole, Carbnicillin, Rifaximin, Penicillin-G, Ox- olinic acid, Bacitracin, Clindamycin, Erthromycin, Novamicin and Aztreomycin. The Ciprofloxacin and Aztreomycin have high activity at both acidic and neutral pH, while the Amikcin, Meromenem, Imipenem and Ciprofloxacin have high activity at basicity pH against these bacteria.

scholarly journals Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

2020 ◽  
pp. 68-73
Keyword(s):  
Developing Countries ◽  
Culture Media ◽  
Bacterial Species ◽  
Positive Culture ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Ear Infection ◽  
Pseudomonas Spp ◽  
Multiple Drugs ◽  
Klebsiella Spp

Children are more susceptible to ear infection than adults worldwide, especially in developing countries. In Bangladesh like other developing countries antibiotics is a common choice of treatment in an ear infection. This study was sought to determine causative agents both in right and left ear infection and their antibacterial susceptibility pattern from patients with an ear infection. Specimens of ear discharge were collected aseptically using a sterile swab and cultured on MacConkey’s agar, blood agar, and chocolate agar. After inoculating on appropriate culture media bacterial isolates were identified by their colony morphology, culture characteristics, Gram reaction, and biochemical tests. In this study, a total of 70 positive cases of patients were included. Among these 27 (38.6%) were male, and 43(61.4%) were female. The prevalence of ear infection was significantly high in females (61.4%) than males (38.6%). The predominant bacterial isolates from positive culture were Staphylococcus aureus 43(61.4%), followed by Pseudomonas spp., 13 (18.6%), Streptococcus spp., 8(11.4%), Proteus spp., 5(7.2%), and Klebsiella spp., 1(1.4%). The antibacterial agent like Amikacin and Gentamicin showed a high level of antibacterial effect on all identified bacterial isolates. On the other hand, 98.6% of isolates were found highly resistant to Co-Trimoxazole and Flucloxacillin. Moreover, Streptococcus spp., Proteus spp., Klebsiella spp., and Pseudomonas spp., were highly resistant to multiple drugs (more than 4). A high degree of antibiotic resistance was observed among most of the drugs used in this study. However, Amikacin and Gentamicin were highly effective against the isolated bacterial species. Therefore, culture and susceptibility tests are vital for the appropriate treatment of ear infection.

scholarly journals Prevalence of Antimicrobial Resistance in Bacterial Isolates from Dogs and Cats in a Veterinary Diagnostic Laboratory in Colombia from 2016–2019

2020 ◽  
Vol 7 (4) ◽  
pp. 173
Author(s):  
David A. Gómez-Beltrán ◽  
David Villar ◽  
Sara López-Osorio ◽  
Duncan Ferguson ◽  
Laura K. Monsalve ◽  
...  
Keyword(s):  
Multi Drug Resistance ◽  
Bacterial Isolates ◽  
Intrinsic Resistance ◽  
Diagnostic Laboratory ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Amoxicillin Clavulanate ◽  
Tract Infections ◽  
Staphylococcus Spp

The susceptibility to antimicrobials of bacterial isolates from dogs (n = 1256) and cats (n = 94) was retrospectively evaluated in a veterinary diagnostic laboratory over a 4-year period (2016–2019). Out of 1316 isolates in dogs, 771 were Staphylococcus spp. distributed as follows: Staph. pseudointermedius (n = 406), Staph. intermedius (n = 160), Staph. aureus (n = 104), and Staph. coagulase-negative (n = 101). In common, all Staphylococcus spp. showed a high prevalence (20–50%) of resistance to ampicillin, cephalosporin, enrofloxacin, gentamicin, tetracycline, and trimethoprim-sulfonamide, but a low prevalence (1–10%) of resistance to amoxicillin-clavulanate. With regards to the other families of bacteria, the number of antimicrobials for which resistance was high (>20%) in dogs was: Enterobacteriaceae (7/12), Enterococcus spp. (10/16), E. coli (11/15), Pseudomonas spp. (10/13), and Streptococcus spp. (4/9). For urinary tract infections caused by E. coli or Enterobacteriaceae (Klebsiella spp., Proteus spp.), amikacin and florfenicol were the only drugs that demonstrated 100% in vitro efficacy. Multi-drug resistance was observed in 18.7% (246/1316) and 22% (21/97) of the isolates from dogs and cats, respectively. Except for Pseudomonas spp., known for intrinsic resistance, resistance in other bacteria was likely attributed to high selection pressure. In conclusion, empirical antimicrobial use cannot be recommended to treat most common infections, and selection should be based on results from susceptibility testing.

scholarly journals ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM SOME SPOILED FRUITS AND VEGETABLES COLLECTED FROM NAGAON, ASSAM

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dipanjali Devi ◽  
Rupjyoti Gogoi
Keyword(s):  
Fruits And Vegetables ◽  
Sufficient Condition ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Great Loss ◽  
Local Markets

Fruits and vegetables provide sufficient condition for the growth of several fungi and bacteria Microorganisms spoil the fruits and vegetables and changes the texture and makes them uneatable. There is a heavy loss of fruits and vegetables due to spoilage by microorganisms; however during handling and transportation also, there is a greater risk to get damaged .Thus farmers suffered a great loss due to spoilage. In the present study a total of about 3 spoiled fruits and 3spoiled vegetables were taken from some local markets of Nagaon, from which fungal and bacterial isolates were isolated and identified. Biochemical tests for the isolated bacteria were also done.

scholarly journals Microbial Incidence and Antibiotic susceptibility for Bacterial isolates in The Mobile Phone of Healthcare workers and University Employments in Basrah City

2020 ◽  
Vol 14 (3) ◽  
pp. 1863-1870
Author(s):  
Rana Mohammad Abd-ulnabi ◽  
Zuhair G. Alshaheen ◽  
Rafeef A. Abdul-jabbar
Keyword(s):  
Mobile Phone ◽  
Mobile Phones ◽  
Healthcare Workers ◽  
Pathogenic Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Candida Spp ◽  
Significant Difference ◽  
Field Samples ◽  
Bacillus Spp

The mobile phones come to be an important device all people use it for communication and phones can be contaminated with bacterial isolates. this study tries to do a little investigation about kinds and percent of pathogenic bacteria and the detection of some antibiotic resistance one and difference between that found in the healthcare worker’s mobile phone and peoples who work out of that field. Samples collection were done using mobile phones belongs to healthcare workers and Basra University employments. Swabs were cultured on different media then the suspected colonies were identified by recognizing the morphology of colonies, staining with Gram’s stain then tested with biochemical tests. Our study shows bacterial isolates in all the screened mobile phone, The highest proportions were recorded by Bacillus spp (66%) and Pseudomonas spp (56%) while the lowest proportions were Staphylococcus aureus (8%) and Candida spp (12%). And there was a significant difference in the kinds and percent of bacterial isolates between the healthcare workers and employments mobile phones, besides the appearance of Cefotaxime and Cefatizoxime resistance Proteus sp. and Trimethoprim + Sulfamethoxazole resistance Klebsiella spp. also the intermediate susceptibility to the different antibiotic was shown in all isolates. All the mobile phones can be contaminated by various kinds of bacteria some of these were resistant to some antibiotics and there was a significant difference in the percent and kinds of bacterial isolates between the health care worker and people who work out of this field.

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