scholarly journals THE CAPABILITY OF SEDATIVE EFFECT FROM CELERY (Apium graveolens L.) FRACTION TO MALE MICE

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Shinta Kusuma ◽  
Arum Setiawan ◽  
Salni Salni
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Water Fraction ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Traction Test ◽  
Effect Test

Celery fraction research (Apium graveolens L.) was carried out to determine the ability of the sedation effect of celery fraction compared to celery extracts which have been known to have the ability to effect the previous sedation. This study aims to find out which fraction has the best sedation effect. This study was an experimental study with a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Test animals divided into 5 treatment groups namely negative control group (CMC Na 1%), celery extract group 200mg /kg and 3 treatment groups n-hexane fraction, ethyl acetate, and methanol water fraction with a dose of 200 mg /kg . The sedation effect test was carried out using the Traction Test and Fireplace Testmethods. Quantitative data observed were the length of time the mice fell and the length of time the mice went out of the heated tube/glass. The results of the analysis showed that the celery fraction had a better sedation effect than the extract, and the methanol water fraction 200 mg/kg was the most effective fraction in causing sedation effects.

scholarly journals AIR GANDARUSA (Justicia gendarussa Burm. f.) DAN GAMBARAN GEN HYALURONIDASE LEWAT ANALISIS PCR

2018 ◽  
Vol 19 (2) ◽  
pp. 69
Author(s):  
Sri Lestari Utami ◽  
Didik P. Restanto ◽  
Bambang Prajogo EW
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Pcr Analysis ◽  
Control Group ◽  
Total Rna ◽  
Water Fraction ◽  
Treatment Groups ◽  
Verification Process ◽  
Per Oral

Gandarusa (J. gendarussa Burm. f.) is an etnomedicine which is used as a male contraceptive alternative, known to inhibit the enzymefunction of spermatozoa hyaluronidase during fertilization. One of the ideal contraceptive conditions is the safetyness (among others nonmutagenic), requiring a long research during the verification process. The early research is the gene expression of hyaluronidase mice(M.musculus L.) testis given water fraction of the gandarusa with PCR analysis. The total RNA was isolated from the normal mice testis(as the negative control or no treatment group) and the mice testis from the treatment groups. The treatment groups consisted of group Iand II treated with water fraction of the gandarusa 15 mg/20 gr BW and 7.5 mg/20 gr BW, subsequently, the positive control group wasalso given hesperidins 1 mg/20 gr BW once a day per oral during the 1.5 times of spermatogenesis cycle (for 55 days). The results of thestudy showed that (1) the cDNA fragment confirmed as the gene of hyaluronidase mice testis (with 710 bp length of nucleotide) passedthrough RT-PCR at the total RNA negative control group, sequenced, isolated and alignment in the NCBI gene bank. (2) the same cDNAfragment gene of hyaluronidase mice testis (from the negative control group) did not transcript in the treatment I and positive controlgroups (where there is no band), but this gene will be transcripted in the treatment II group (where the band is emerged).

scholarly journals PENGARUH EKSTRAK DELIMA MERAH(Punicagranatum L.) TERHADAP PENINGKATAN PERSENTASE KAPASITASI DAN REAKSI AKROSOM SPERMATOZOA TIKUS PUTIH (Rattusnorvegicus)

2020 ◽  
Vol 8 (2) ◽  
pp. 100
Author(s):  
Baktiar Budi Satrio ◽  
Mas’ud Hariadi ◽  
Retno Sri Wahjuni ◽  
Budi Utomo ◽  
Sri Agus Sudjarwo ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
Acrosome Reaction ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Randomized Design ◽  
Solvent Control

The aim of this research is to know the effect of pomegranate (Punicagranatum L.) on the improvement of spermatozoa quality that is increase of percentage of capacitation and acrosome reaction in white rat (Rattusnorvegicus). This research used 20 healthy white rat (Rattusnorvegicus) with average age 2-3 months old, 200-300 g. Research design using completely randomized design (CRD) with four treatments and 5 replications. Rats were randomly divided into 4 treatment groups. Rat in the negative control group not treated just kept alone, solvent control group were given CMC Na 0,5% and is exposed to heat, the treatment groups 1, 2, and 3 induced pomegranate extract standardized to 40% ellagic acid at a dose of 75mg / kg, 150mg / kg and 300mg /kg perorally with doses 1 ml/rat/day gastric for along 14 days and exposed to heat. Data were analyzed using One Way Analysis of Variant (ANOVA) followed by Duncan (p<0,05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa especially to capacitation and acrosome reaction. The treatment from treatment 2 group showed the best result in maintaining the quality of rats spermatozoa with 27.6± 3.04 values for capacitation and 6,80 ± 1,30 for acrosome reactions. Extract of pomegranate (Punicagranatum L.) standardized to 40% ellagic acid has a significant change in improve the quality of spermatozoa, namely an increase in the percentage of capacitation and acrosome reactions in white rats (Rattusnorvegicus) exposed to heat.

scholarly journals Analgesic Activity of Ethanol Extract of Temu Giring Rhizome (Curcuma heyneana Val & Zijp) in Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 9-13
Author(s):  
Marianne ◽  
Khairunnisa ◽  
Wilda
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Significance Level ◽  
Treatment Groups ◽  
Infra Red ◽  
Thermal Induction

Temu giring (Curcuma heyneana Val & Zijp) is a traditional medicinal plant that is believed in community as an analgesic. The objective of this research was to determine the analgesic activity of the C. heyneana rhizome by using infra red (IR) thermal induction method in mice. Mice were divided into 7 groups. Group 1 served as negative control, group 2,3,4,5 served as treatment groups which is  given ethanolic extract of C. heyneana rhizome at  dose of 5, 25, 125, and 625 mg/kg respectively, group 6 and 7 served as  comparable groups, given antalgin 65 mg/kg and morphine sulphate 1.3 mg/kg respectively. The observation have been done, included to pain resistance of mice which exposed by infra red (IR) every 10 minutes for 80 minutes. The data were analyzed by ANOVA at the significance level of 95%. Ethanolic extract of C. heyneana at the doses of 25, 125, and 625 mg/kg had significant effect to reduce the pain compared to the negative control (p<0.05). Ethanolic extract of C. heyneana rhizome at dose of 125 mg/kg, had the same effect to antalgin 65 mg/kg  (p≥0.05), while the ethanolic extract of C. heyneana at the dose of 625 mg/kg had the same effect as morphine sulfate 1.3 mg/kg (p≥0.05). It can be concluded that ethanolic extract of C. heyneana rhizome has analgesic activity.   Keywords: temu giring, analgesic, Curcuma heyneana, rhizome

scholarly journals Percepatan Penutupan Luka Sayat pada Tikus Putih Akibat Pemberian Perasan Rimpang Temulawak (Curcuma xanthorrhiza Roxb.)

2021 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Elisabeth Natalia Barung ◽  
Rifny Wungow ◽  
Donald Emilio Kalonio
Keyword(s):  
Wound Healing ◽  
Linear Regression Analysis ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
White Rats ◽  
Curcuma Xanthorrhiza ◽  
Incision Wound

Rimpang Temulawak atau Curcuma xanthorriza Roxb. adalah tanaman yang dikenal luas oleh masyarakat sebagai obat tradisional. Rimpang temulawak mengandung kurkumin dan xanthorrizzol, yang diketahui mampu mempercepat penutupan luka di kulit dan juga memiliki efek antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk untuk mengetahui efektifitas perasan rimpang temulawak terhadap percepatan penutupan luka sayat pada tikus putih. Penelitian ini adalah penelitian eksperimen, dengan subyek 10 ekor tikus putih yang dibagi dalam 2 kelompok perlakuan yaitu kelompok yang diberi perasan temulawak dan kelompok kontrol negatif yang tidak diberi perlakuan. Data dikumpulkan dengan mengukur panjang luka tikus menggunakan alat ukur penggaris dan dihitung persentasi penutupan luka. Data dianalisis menggunakan analisis regresi linear dan nilai slope (b) dinyatakan sebagai kecepatan penutupan luka. Hasil penelitian ini, menunjukan bahwa rimpang temulawak mampu mempercepat penutupan luka sayat sebesar 15,262%/hari dibandingkan kelompok yang tidak diberi perlakuan sebesar 13,54%/hari. Kata Kunci: Perasan Rimpang Temulawak, Luka Sayat, Percepatan Penutupan Luka, Sediaan Sederhana, Obat Tradisional Indonesia Curcuma xanthorrhiza Roxb. is a plant that is widely known by the community as traditional medicine. The rhizome of C. xanthorrhiza contains curcumin and xanthorrhizol, which are known to be able to accelerate wound healing on the skin and also has antibacterial and anti-inflammatory effects. This study was aimed to determine the effectiveness of C. xanthorriza rhizome on the acceleration of incision wound healing on white rats. This study was an experimental study, with 10 white rat subjects divided into two treatment groups, namely the treatment group which was given by C. xanthorriza rhizome and the negative control group that was not treated. Data were collected by measuring rat wound length using a ruler and calculating the percentage of wound healing. By using linear regression analysis and the value of the slope (b) is expressed as the acceleration of wound healing. The results of the study showed that C. xanthorrhiza rhizome was able to accelerate incision wound healing by 15.262% / day compared to the untreated group of 13.54% / day.

scholarly journals GAMBARAN HISTOLOGIK JARINGAN LIMPA TIKUS PUTIH (Rattus norvegicus) YANG DIINFEKSI ESCHERICIA COLI DAN DIBERI MADU

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Christiani Matheos
Keyword(s):  
Sugar Content ◽  
Negative Control Group ◽  
Blood Stream ◽  
Negative Control ◽  
Control Group ◽  
Spleen Tissue ◽  
Treatment Groups ◽  
E Coli ◽  
Histological Picture ◽  
High Level

Abstrak: Daya antimikroba dalam madu dipengaruhi oleh kandungan glukosanya yang tinggi, tingkat keasaman madu, serta senyawa flavonoid yang dapat mengikat radikal bebas dan melawan agen infeksi. Eschericia coli dapat menimbulkan infeksi lokal organ tubuh, mencapai aliran darah, dan menimbulkan sepsis. Penelitian ini bertujuan untuk mengetahui perubahan gambaran histologik jaringan limpa tikus yang diinfeksi E.coli dan diberi madu. Penelitian ini merupakan penelitian eksperimental menggunakan 16 ekor tikus putih yang dibagi dalam satu kelompok kontrol negatif (KN) dan tiga kelompok perlakuan (KP). Kelompok kontrol negatif diberi pelet biasa selama 14 hari; KP 1 diberi pelet dan madu 2 mL/hari selama 35 hari; KP 2 diberi pelet dan E. coli 1 mL/hari melalui selama 7 hari; serta KP3 diberi pelet, E. coli 1 mL/hari selama 7 hari, dan madu 2 mL/hari selama 35 hari. Organ limpa tikus diproses untuk dibuat sediaan jaringan histologi. Jaringan limpa tikus KN dan KP1 menunjukkan gambaran histologik yang sesuai dengan limpa normal. Jaringan limpa tikus KP2 menunjukkan kongesti akut dan vasodilatasi pembuluh darah, sedangkan pada KP3 tampak kongesti yang lebih ringan dari yang terlihat pada KP2 dan adanya tanda regenerasi sel-sel limfohematopoietik jaringan limpa. Dengan demikian dapat disimpulkan bahwa kelompok perlakuan yang diberi E.coli tanpa pemberian madu menunjukkan suatu splenitis akut non spesifik, sedangkan kelompok perlakuan yang diberi E.coli dengan pemberian madu menunjukkan tanda-tanda perbaikan dari radang dan tanda regenerasi sel.Kata kunci: Madu, Eschericia coli, Limpa.Abstract: Antimicrobial force in his sugar content of honey is influenced by the high level of acidity of honey, as well as flavonoid that can bind free radicals and fight the infection agent. Eschericia coli can cause local infection of the body's organs, reaching the blood stream, and give rise to sepsis. This study aimed to determine changes in histological picture of the spleen tissue of mice given infected E.coli and gave honey. An experimental study using 16 rats divided into one negative control group (KN) and tree treatment groups (KP). Negative control group given normal pellets for 14 days. KP1 is given pellets and honey 2 mL / day for 35 days. KP2 are given pellets and E. coli 1 mL / day through 7 days. KP3 given pellets, E. coli 1 mL / day for 7 days, and honey for 35 days. Mouse splenic processed and examined through a microscope. Mice of KN network and KP1 showed normal histological picture of the spleen. Spleen of KP 2 show today showed acute congestion and vasodilation of blood vessels spleen. Spleen of KP3 show a little more congestion and an indication of regeneration.It can be concluded that the treatment groups were given E. coli without giving honey showed a nonspecific acute splenitis, whereas the treatment group given E. coli with honey granting remission to show signs of inflammation.Key words: Honey, Eschericia coli, Spleen.

scholarly journals Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

2021 ◽  
Vol 4 (2) ◽  
pp. 73
Author(s):  
Putri Ayu Ika Setiyowati ◽  
Rofiatun Solekha ◽  
Sri Bintang Sahara Mahaputra Kusuma Negara ◽  
Reny Rosalina
Keyword(s):  
Body Weight ◽  
Optimal Dose ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Optimal Dosage ◽  
Skin Injury ◽  
Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

scholarly journals Blood profile and gut microbial load of broilers fed siam weed (Chromolaena odorata) leaf meal in their diets

Agricultura ◽  
2017 ◽  
Vol 14 (1-2) ◽  
pp. 17-24 ◽  
Author(s):  
Peter-Damian Chukwunomso Jiwuba ◽  
Ifeanyi Princewill Ogbuewu ◽  
Elisha Dauda ◽  
Caritas Chibuike Azubuike
Keyword(s):  
Immune Status ◽  
Microbial Load ◽  
Control Group ◽  
Pathogenic Microorganisms ◽  
Chromolaena Odorata ◽  
Blood Profile ◽  
Treatment Groups ◽  
Leaf Meal ◽  
Randomized Design ◽  
Completely Randomized Design

AbstractThe responses of 96 day old broilers fed Chromolaena odorata leaf meal (COLM) on blood profile and gut microbial load were studied for 49 days. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The birds were fed four experimental diets formulated at 0%, 2%, 4% and 6% for the starter phase and 0%, 4%, 8% and 12% of COLM at the finisher phase for T1, T2, T3 and T4 respectively. All the haematological parameters were similar (P>0.05) across the treatment groups except MCH and WBC which were significantly (P< 0.05) higher and better in the treatment groups than the control group. The results on serum chemistry showed significant (P<0.05) difference for total protein, albumin, creatinine and cholesterol across the treatment groups. Cholesterol was significantly (P<0.05) reduced with increasing levels of COLM. Results showed that coliforms and E.coli were consistently higher (P<0.05) among the T1 birds than those fed T2, T3 and T4. It was concluded that, COLM enhanced adequate haematocrit and immune status, hypoglycaemic ability, suppresses the growth of gut pathogenic microorganisms and enhanced the growth of beneficial microorganism in broilers.

scholarly journals Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan

2021 ◽  
Vol 9 (2) ◽  
pp. 79
Author(s):  
Febriyono Anggela Prakoso ◽  
Raden Tatang Santanu Adikara ◽  
Kusnoto Kusnoto ◽  
Sri Hidanah ◽  
Yeni Dhamayanti ◽  
...  
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Research Methods ◽  
Control Group ◽  
Treatment Groups ◽  
Gain Rate ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bali Cattle

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.

In vivo Antitumoral Effect of Plantago major L. Extract on Balb/C Mouse with Ehrlich Ascites Tumor

2007 ◽  
Vol 35 (05) ◽  
pp. 841-851 ◽  
Author(s):  
Mehmet Ozaslan ◽  
I. Didem Karagöz ◽  
M. Emin Kalender ◽  
I. Halil. Kilic ◽  
Ibrahim Sari ◽  
...  
Keyword(s):  
Weight Gain ◽  
Negative Control Group ◽  
Negative Control ◽  
Ehrlich Ascites Tumor ◽  
Control Group ◽  
Plantago Major ◽  
Ascites Tumor ◽  
Treatment Groups ◽  
Ehrlich Ascites

The aim of this study is to investigate the antitumor activity of Plantago major L. extract in Ehrlich ascites tumor (EAT) bearing Balb/C mice in vivo. Thirty male Balb/C mice were divided into 5 groups: 3 treatment groups and 2 control groups (6 per group). Treatment groups and the negative control group were injected with EAT (1 × 106 cells) intraperitoneally to develop ascites tumor. P. major L. extract (1%, 2% and 3% concentration extracts, 0.1 ml/day/mouse) were given p.o. for 10 alternate days. The control group was treated with 0.9% NaCl solution (0.1 ml/day/mouse). The changes of body weight in animals were recorded. On the 11th day, all of the mice were sacrified and their tissues were stained with haematoxylen and eosin for pathological studies. Body weights of in 3 treatment groups and the negative control group were elevated because of tumor burden. The maximal weight gain was recorded in the negative control group and the minimal weight gain was recorded in Group I. Pathological studies showed that P. major L. extract (especially 1% concentration) has inhibitive effect on EAT. P. major has an inhibitory effect on EAT in a dose dependent manner.

scholarly journals The bone strengthening effects of propolis in ovariectomized female white rats as models for postmenopause

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Dian Ayu Juwita ◽  
Almahdy Almahdy ◽  
Rahmad Abdillah ◽  
Fiony Syahputri
Keyword(s):  
Bone Strength ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Impact Testing ◽  
Control Group ◽  
Test Results ◽  
Treatment Groups ◽  
White Rats

Abstract Osteoporosis is a bone disease characterized by decreased quality and strength of bones so that it becomes porous and fracture. Propolis is known to have many pharmacological activity, including an anti-osteoporosis effect. This study aims to determine the effect of propolis administration and the effects of propolis dosage variation in preventing osteoporosis based on the strength value of femur bone impact in female white rats in the form of an ovariectomy postmenopausal model. The rats were divided into 5 groups: positive control group (subjected to ovariectomy), negative control group (not subjected to ovariectomy, and treatment groups that were subjected to ovariectomy and given propolis at a dose of 180 mg/kg BW, dose 360 mg/kg BW and dose 720 mg/kg BW. Propolis was administered orally for 30 days. Bone impact strength testing was undertaken after 30 days using an impact testing machine. Research data were analyzed via one-way ANOVA and continued with the Duncan’s Multiple Range Test. From the test results, we noted that propolis administration had an effect on the value of bone strength, with the dose of 720 mg/kg BW and 360 mg/kg BW having a significant effect, compared with others. With an increase in dose, propolis can provide an increase in the value of bone strength in rat bones.

Sign in / Sign up

Export Citation Format

Share Document