scholarly journals PENGARUH EKSTRAK DELIMA MERAH(Punicagranatum L.) TERHADAP PENINGKATAN PERSENTASE KAPASITASI DAN REAKSI AKROSOM SPERMATOZOA TIKUS PUTIH (Rattusnorvegicus)

2020 ◽  
Vol 8 (2) ◽  
pp. 100
Author(s):  
Baktiar Budi Satrio ◽  
Mas’ud Hariadi ◽  
Retno Sri Wahjuni ◽  
Budi Utomo ◽  
Sri Agus Sudjarwo ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
Acrosome Reaction ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Randomized Design ◽  
Solvent Control

The aim of this research is to know the effect of pomegranate (Punicagranatum L.) on the improvement of spermatozoa quality that is increase of percentage of capacitation and acrosome reaction in white rat (Rattusnorvegicus). This research used 20 healthy white rat (Rattusnorvegicus) with average age 2-3 months old, 200-300 g. Research design using completely randomized design (CRD) with four treatments and 5 replications. Rats were randomly divided into 4 treatment groups. Rat in the negative control group not treated just kept alone, solvent control group were given CMC Na 0,5% and is exposed to heat, the treatment groups 1, 2, and 3 induced pomegranate extract standardized to 40% ellagic acid at a dose of 75mg / kg, 150mg / kg and 300mg /kg perorally with doses 1 ml/rat/day gastric for along 14 days and exposed to heat. Data were analyzed using One Way Analysis of Variant (ANOVA) followed by Duncan (p<0,05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa especially to capacitation and acrosome reaction. The treatment from treatment 2 group showed the best result in maintaining the quality of rats spermatozoa with 27.6± 3.04 values for capacitation and 6,80 ± 1,30 for acrosome reactions. Extract of pomegranate (Punicagranatum L.) standardized to 40% ellagic acid has a significant change in improve the quality of spermatozoa, namely an increase in the percentage of capacitation and acrosome reactions in white rats (Rattusnorvegicus) exposed to heat.

scholarly journals Percepatan Penutupan Luka Sayat pada Tikus Putih Akibat Pemberian Perasan Rimpang Temulawak (Curcuma xanthorrhiza Roxb.)

2021 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Elisabeth Natalia Barung ◽  
Rifny Wungow ◽  
Donald Emilio Kalonio
Keyword(s):  
Wound Healing ◽  
Linear Regression Analysis ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
White Rats ◽  
Curcuma Xanthorrhiza ◽  
Incision Wound

Rimpang Temulawak atau Curcuma xanthorriza Roxb. adalah tanaman yang dikenal luas oleh masyarakat sebagai obat tradisional. Rimpang temulawak mengandung kurkumin dan xanthorrizzol, yang diketahui mampu mempercepat penutupan luka di kulit dan juga memiliki efek antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk untuk mengetahui efektifitas perasan rimpang temulawak terhadap percepatan penutupan luka sayat pada tikus putih. Penelitian ini adalah penelitian eksperimen, dengan subyek 10 ekor tikus putih yang dibagi dalam 2 kelompok perlakuan yaitu kelompok yang diberi perasan temulawak dan kelompok kontrol negatif yang tidak diberi perlakuan. Data dikumpulkan dengan mengukur panjang luka tikus menggunakan alat ukur penggaris dan dihitung persentasi penutupan luka. Data dianalisis menggunakan analisis regresi linear dan nilai slope (b) dinyatakan sebagai kecepatan penutupan luka. Hasil penelitian ini, menunjukan bahwa rimpang temulawak mampu mempercepat penutupan luka sayat sebesar 15,262%/hari dibandingkan kelompok yang tidak diberi perlakuan sebesar 13,54%/hari. Kata Kunci: Perasan Rimpang Temulawak, Luka Sayat, Percepatan Penutupan Luka, Sediaan Sederhana, Obat Tradisional Indonesia Curcuma xanthorrhiza Roxb. is a plant that is widely known by the community as traditional medicine. The rhizome of C. xanthorrhiza contains curcumin and xanthorrhizol, which are known to be able to accelerate wound healing on the skin and also has antibacterial and anti-inflammatory effects. This study was aimed to determine the effectiveness of C. xanthorriza rhizome on the acceleration of incision wound healing on white rats. This study was an experimental study, with 10 white rat subjects divided into two treatment groups, namely the treatment group which was given by C. xanthorriza rhizome and the negative control group that was not treated. Data were collected by measuring rat wound length using a ruler and calculating the percentage of wound healing. By using linear regression analysis and the value of the slope (b) is expressed as the acceleration of wound healing. The results of the study showed that C. xanthorrhiza rhizome was able to accelerate incision wound healing by 15.262% / day compared to the untreated group of 13.54% / day.

scholarly journals THE CAPABILITY OF SEDATIVE EFFECT FROM CELERY (Apium graveolens L.) FRACTION TO MALE MICE

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Shinta Kusuma ◽  
Arum Setiawan ◽  
Salni Salni
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Water Fraction ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Traction Test ◽  
Effect Test

Celery fraction research (Apium graveolens L.) was carried out to determine the ability of the sedation effect of celery fraction compared to celery extracts which have been known to have the ability to effect the previous sedation. This study aims to find out which fraction has the best sedation effect. This study was an experimental study with a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Test animals divided into 5 treatment groups namely negative control group (CMC Na 1%), celery extract group 200mg /kg and 3 treatment groups n-hexane fraction, ethyl acetate, and methanol water fraction with a dose of 200 mg /kg . The sedation effect test was carried out using the Traction Test and Fireplace Testmethods. Quantitative data observed were the length of time the mice fell and the length of time the mice went out of the heated tube/glass. The results of the analysis showed that the celery fraction had a better sedation effect than the extract, and the methanol water fraction 200 mg/kg was the most effective fraction in causing sedation effects.

scholarly journals The bone strengthening effects of propolis in ovariectomized female white rats as models for postmenopause

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Dian Ayu Juwita ◽  
Almahdy Almahdy ◽  
Rahmad Abdillah ◽  
Fiony Syahputri
Keyword(s):  
Bone Strength ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Impact Testing ◽  
Control Group ◽  
Test Results ◽  
Treatment Groups ◽  
White Rats

Abstract Osteoporosis is a bone disease characterized by decreased quality and strength of bones so that it becomes porous and fracture. Propolis is known to have many pharmacological activity, including an anti-osteoporosis effect. This study aims to determine the effect of propolis administration and the effects of propolis dosage variation in preventing osteoporosis based on the strength value of femur bone impact in female white rats in the form of an ovariectomy postmenopausal model. The rats were divided into 5 groups: positive control group (subjected to ovariectomy), negative control group (not subjected to ovariectomy, and treatment groups that were subjected to ovariectomy and given propolis at a dose of 180 mg/kg BW, dose 360 mg/kg BW and dose 720 mg/kg BW. Propolis was administered orally for 30 days. Bone impact strength testing was undertaken after 30 days using an impact testing machine. Research data were analyzed via one-way ANOVA and continued with the Duncan’s Multiple Range Test. From the test results, we noted that propolis administration had an effect on the value of bone strength, with the dose of 720 mg/kg BW and 360 mg/kg BW having a significant effect, compared with others. With an increase in dose, propolis can provide an increase in the value of bone strength in rat bones.

scholarly journals Utilization of Honey Apis dorsata as Antiosteoporosis on Requirements of Bone Calcium Ash Density on Ovariohysterectomized White Rat (Ratus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 627 ◽  
Author(s):  
Muhammad Huda Ramadhan Ibrahim ◽  
Abdullah Hasib Hasib ◽  
Siti Nur Rohmah ◽  
Salsabilla Abani ◽  
Samsi Yordan ◽  
...  
Keyword(s):  
Lumbar Vertebrae ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ash Content ◽  
Control Group ◽  
Apis Dorsata ◽  
Treatment Groups ◽  
White Rats ◽  
Bone Calcium

The purpose of this study was to determine the effect of honey bee Apis dorsata as anti-osteoporosis in calcium ash density (CAD) of bone in osteoporotic-induced rats. The target of this study was to know bone calcium levels after being given honey bees Apis dorsata. In this study, 35 female white rats (Ratus norvegicus) mature was used with weight 200gr. Divided into 5 groups, 2 control groups and 3 treatment groups. The negative control group (SH) was not induced by osteoporosis and was given the only aquadest of 1.5 ml/day. Whereas the positive control group was induced by osteoporosis (OH)  and was given only aquadest 1.5 ml/day. T1, T2 and T3 treatment groups were given bee honey with various doses including 1g / kg ad 1.5 ml aquadest, 2g / kg BB ad 1.5 ml aquadest and 3g / kg BB ad 1.5 ml aquadest. Then after 12 weeks, white rats were sacrificed for lumbar vertebrae. Furthermore, the sixth lumbar spine os vertebrae will be examined for calcium bone ash content. The data were obtained was analyzed using statistical analysis of variance (ANOVA) for the results of the calcium ash content data Keywords: Honey; Osteoporosis; CAD

scholarly journals AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Devy Angreani M ◽  
Meiske Sangi ◽  
Feti Fatimah
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Wistar Strain ◽  
Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

scholarly journals Histopathology of Heart Rats (Rattus norvegicus) Due to the Use of Cooking Oil

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Siti Aisyah ◽  
Ummu Balqis ◽  
Eko Karunia Friyan
Keyword(s):  
Rattus Norvegicus ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Test Results ◽  
Treatment Groups ◽  
Cooking Oil ◽  
White Rats ◽  
Ad Libitum ◽  
Group B

This research aims to study the histopathological picture of rats (Rattus norvegicus) heart as a result of the provision of waste cooking oil3x, 6x, and 9x frying. This study used 20 male white rats (Rattus norvegicus) weighing of ± 300 g. Rats were grouped into 4 treatment groups,adapted for one week, feed and drink ad libitum. The second week to eight week rats were given orally cooking oil every day with a dose of 1 ml. A group of rats was given cooking oil (negative control). Group B, C, and D were given cooking oil 3x, 6x, and 9x frying. Mi ce were euthanizedafter 60 days, then necropsied to collect the heart. Heart was then put into Neutral Buffered Formalin (NBF) for fixation, then follow withhistopathology preparation. Parameters measured were hyperemia, hemorrhage, degeneration, necrosis of the heart. Results were analyzeddescriptively with the scoring system. Test results are grouped into three categories, namely, mild (+), moderate (++), and weight (+++). Thehystopathological results of the heart found were an increasing number of hyperemia, hemorrhage, degeneration, necrosis as a result of thefrequency of frying.____________________________________________________________________________________________________________________Key words: heart, cooking oil, rats 

scholarly journals Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

2021 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
Alvyan Lantang Anugrah ◽  
Hana Eliyani ◽  
Budi Utomo ◽  
Suherni Susilowati ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Sertoli Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Beet Root ◽  
White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

scholarly journals HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

2019 ◽  
pp. 14
Author(s):  
I Made Merdana ◽  
I Made Kardena ◽  
Ketut Budiasa ◽  
I Made Dodi Gunawan
Keyword(s):  
Rat Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Significant Difference ◽  
Ant Nest ◽  
Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in  hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Analgesic Activity of Ethanol Extract of Temu Giring Rhizome (Curcuma heyneana Val & Zijp) in Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 9-13
Author(s):  
Marianne ◽  
Khairunnisa ◽  
Wilda
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Significance Level ◽  
Treatment Groups ◽  
Infra Red ◽  
Thermal Induction

Temu giring (Curcuma heyneana Val & Zijp) is a traditional medicinal plant that is believed in community as an analgesic. The objective of this research was to determine the analgesic activity of the C. heyneana rhizome by using infra red (IR) thermal induction method in mice. Mice were divided into 7 groups. Group 1 served as negative control, group 2,3,4,5 served as treatment groups which is  given ethanolic extract of C. heyneana rhizome at  dose of 5, 25, 125, and 625 mg/kg respectively, group 6 and 7 served as  comparable groups, given antalgin 65 mg/kg and morphine sulphate 1.3 mg/kg respectively. The observation have been done, included to pain resistance of mice which exposed by infra red (IR) every 10 minutes for 80 minutes. The data were analyzed by ANOVA at the significance level of 95%. Ethanolic extract of C. heyneana at the doses of 25, 125, and 625 mg/kg had significant effect to reduce the pain compared to the negative control (p<0.05). Ethanolic extract of C. heyneana rhizome at dose of 125 mg/kg, had the same effect to antalgin 65 mg/kg  (p≥0.05), while the ethanolic extract of C. heyneana at the dose of 625 mg/kg had the same effect as morphine sulfate 1.3 mg/kg (p≥0.05). It can be concluded that ethanolic extract of C. heyneana rhizome has analgesic activity.   Keywords: temu giring, analgesic, Curcuma heyneana, rhizome

Sign in / Sign up

Export Citation Format

Share Document