scholarly journals Blood profile and gut microbial load of broilers fed siam weed (Chromolaena odorata) leaf meal in their diets

Agricultura ◽  
2017 ◽  
Vol 14 (1-2) ◽  
pp. 17-24 ◽  
Author(s):  
Peter-Damian Chukwunomso Jiwuba ◽  
Ifeanyi Princewill Ogbuewu ◽  
Elisha Dauda ◽  
Caritas Chibuike Azubuike
Keyword(s):  
Immune Status ◽  
Microbial Load ◽  
Control Group ◽  
Pathogenic Microorganisms ◽  
Chromolaena Odorata ◽  
Blood Profile ◽  
Treatment Groups ◽  
Leaf Meal ◽  
Randomized Design ◽  
Completely Randomized Design

AbstractThe responses of 96 day old broilers fed Chromolaena odorata leaf meal (COLM) on blood profile and gut microbial load were studied for 49 days. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The birds were fed four experimental diets formulated at 0%, 2%, 4% and 6% for the starter phase and 0%, 4%, 8% and 12% of COLM at the finisher phase for T1, T2, T3 and T4 respectively. All the haematological parameters were similar (P>0.05) across the treatment groups except MCH and WBC which were significantly (P< 0.05) higher and better in the treatment groups than the control group. The results on serum chemistry showed significant (P<0.05) difference for total protein, albumin, creatinine and cholesterol across the treatment groups. Cholesterol was significantly (P<0.05) reduced with increasing levels of COLM. Results showed that coliforms and E.coli were consistently higher (P<0.05) among the T1 birds than those fed T2, T3 and T4. It was concluded that, COLM enhanced adequate haematocrit and immune status, hypoglycaemic ability, suppresses the growth of gut pathogenic microorganisms and enhanced the growth of beneficial microorganism in broilers.

scholarly journals Assessment of the nutritive value of fermented cassava root leaf meal as a reflect on blood profile of ducks

2020 ◽  
Vol 47 (3) ◽  
pp. 194-201
Author(s):  
W. A. Olayemi ◽  
I. A. Rabiu ◽  
A. O. Oso ◽  
O. A. Akapo ◽  
A. M. Bamgbose
Keyword(s):  
Health Status ◽  
Nutritive Value ◽  
Control Treatment ◽  
Blood Profile ◽  
Leaf Meal ◽  
Cassava Root ◽  
Randomized Design ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Dietary Treatments

Due to scarcity and high cost of conventional feedstuffs, there is a renewed interest in the use of non-conventional cheap and easily available feedstuffs on ducks feeding. This study was conducted to evaluate health status of ducks fed fermented cassava root-leaf meal blend as a replacement for maize. One hundred and fifty, one day- old unsexed Mallard ducklings were used for the experiment. Ducklings were randomly distributed into 5 groups of 30 ducklings per treatment. Each treatment was further subdivided into 3 replicates with 10 ducklings per replicate in a completely randomized design (CRD). The study is made up of 5 dietary treatments consisting of the control (Treatment 1), fermented cassava root-leaf blend used to replace maize at 25% (Treatment 2), 50% (Treatment 3), 75% (Treatment 4) and 100% (Treatment 5) levels respectively. The packed cell volume (26.50-38.33%) and haemoglobin (9.35-14.07g/dl) levels increased as Fermented cassava root-leaf meal (FCRLM) inclusion increased in the diet (0-75%). Uric acid values decreased (5.30-4,27mg/dl) as well as cholesterol values (91.70-56.70mg/dl) as level of (FCRLM) increased in diet. The results obtained showed that FCRLM at (300:1000 ratio) of leaf to root could replace maize at 50% inclusion level without any detrimental effects on health status of ducks.

scholarly journals THE CAPABILITY OF SEDATIVE EFFECT FROM CELERY (Apium graveolens L.) FRACTION TO MALE MICE

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Shinta Kusuma ◽  
Arum Setiawan ◽  
Salni Salni
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Water Fraction ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Traction Test ◽  
Effect Test

Celery fraction research (Apium graveolens L.) was carried out to determine the ability of the sedation effect of celery fraction compared to celery extracts which have been known to have the ability to effect the previous sedation. This study aims to find out which fraction has the best sedation effect. This study was an experimental study with a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Test animals divided into 5 treatment groups namely negative control group (CMC Na 1%), celery extract group 200mg /kg and 3 treatment groups n-hexane fraction, ethyl acetate, and methanol water fraction with a dose of 200 mg /kg . The sedation effect test was carried out using the Traction Test and Fireplace Testmethods. Quantitative data observed were the length of time the mice fell and the length of time the mice went out of the heated tube/glass. The results of the analysis showed that the celery fraction had a better sedation effect than the extract, and the methanol water fraction 200 mg/kg was the most effective fraction in causing sedation effects.

scholarly journals Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan

2021 ◽  
Vol 9 (2) ◽  
pp. 79
Author(s):  
Febriyono Anggela Prakoso ◽  
Raden Tatang Santanu Adikara ◽  
Kusnoto Kusnoto ◽  
Sri Hidanah ◽  
Yeni Dhamayanti ◽  
...  
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Research Methods ◽  
Control Group ◽  
Treatment Groups ◽  
Gain Rate ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bali Cattle

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.

scholarly journals The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

2020 ◽  
Vol 13 (8) ◽  
pp. 1594-1598
Author(s):  
Muslim Akmal ◽  
Gholib Gholib ◽  
Mustafa Kamal Nasution ◽  
Sri Wahyuni ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Commercial Chicken ◽  
Male Chicken ◽  
Completely Randomized Design ◽  
Kinase A

Background and Aim: Testis (T) and epididymis (E) are waste from the abattoir that is rarely used. In fact, both organs contain important chemicals needed for spermatogenesis (e.g., hormones, proteins, and other molecules). Therefore, administration of a combination of testis and epididymis (CTE) extracts may activate androgen receptors (AR) and protein kinase A (PKA) molecules that play a prominent role in spermatogenesis. We, therefore, aimed at investigating the influence of the CTE extracts on the concentration of AR and PKA in male chicken. Materials and Methods: This study used a completely randomized design with four treatment groups (K0, K1, K2, and K3) and five replications per group. K0 is a control group that received 1 mL normal saline, whereas K1, K2, and K3 are the test groups that received 1, 2, and 3 mL of CET extracts, respectively. Twenty male chickens (strain: broiler Mb 89), 3 weeks of age, weighing 500-700 g were used. We administered the injections in a 13-day period and on the 14th day; we collected and processed blood samples as serum to measure the AR and PKA concentrations using commercial chicken AR and PKA enzyme-linked immunosorbent assay kits, respectively. We performed analyses by analysis of variance using SPSS 20.0. Results: The AR concentrations in K1, K2, and K3 groups increased by 4.26%, 10.97%, and 28.04%, respectively, compared to the K0 (control group). However, this increase was not significantly different between the groups (p>0.05). Moreover, the PKA concentrations increased by 2.97%, 2.60%, and 4.08% in K1, K2, and K3 groups, respectively, compared to the control group. However, this increase was not significantly different between the groups as well (p>0.05). Conclusion: The CTE extracts tended to increase the AR and PKA concentrations even though it is not significant. Therefore, it needs further study when using the CTE extracts for spermatogenesis in male chicken.

scholarly journals Dietary Effect of Velvet Bean (Mucuna utilis) Leaf Meal on Haematology and Serum Biochemistry of Broiler Finisher Birds

2018 ◽  
Vol 2 ◽  
pp. 1-5
Author(s):  
Peter-Damian Chukwunomso Jiwuba ◽  
Emeka Cyril Onunwa
Keyword(s):  
Immune Status ◽  
Serum Biochemistry ◽  
Control Group ◽  
Velvet Bean ◽  
Treatment Groups ◽  
Leaf Meal ◽  
Significant Difference ◽  
Serum Biochemical ◽  
Biochemical Indices ◽  
Effect Of Feeding

A study was conducted to investigate the effect of feeding varying levels of Mucuna utilis leaf meal (MULM) on the haematological and serum biochemistry parameters of broiler finisher birds using ninety six (96) four week old chicks for 29 days. The birds were randomly assigned to the four treatments, each replicated three times with eight (8) birds constituting a replicate. The birds were allotted to four treatments containing 0, 10, 20, and 30% MULM. The haematological parameters evaluated showed no significant difference (P> 0.05) on PCV, Hb, RBC and WBC. MCHC was improved and better at D birds in comparison to other treatments. MCV and MCH differ significantly (P< 0.05) with birds on control showing higher values than those on C and D. Total protein, albumin and globulin showed better (p<0.05) values for the treatment groups when compared with the control group. The study concluded that, on account of adequate haematocrit and immune status, incorporation of MULM at 30% in the diets of broiler finisher birds had no deleterious effect on haematological and serum biochemical indices and therefore recommend for production of healthy broilers.

scholarly journals Effect of feed grade acidifiers on total tract mineral retention, intestinal pH and phytate utilization by broiler finisher chickens

2020 ◽  
Vol 44 (1) ◽  
pp. 282-289
Author(s):  
C. U. Idachaba ◽  
I. Abdullahi ◽  
D. A. Kurtong
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Control Treatment ◽  
Control Group ◽  
Available Phosphorus ◽  
Treatment Groups ◽  
Randomized Design ◽  
Mineral Utilization ◽  
Completely Randomized Design

Mineral chelators such as organic acids have been reported to compete favourably with phytate by forming soluble complexes with feed minerals. Effect of feed grade acidifiers on total tract mineral retention and intestinal pH of broiler finisher chickens was investigated. A total of 300-broiler finisher chickens were assigned to four treatment groups. Each treatment was replicated thrice with twenty chickens per replicate in a completely randomized design. The control treatment (T1 ) was fed the control diet adequate in available phosphorus at 0.5% while other treatments (T2 -T4 ) were fed sub-optimal available phosphorus diets at 0.4%. Three blends of acidifiers; Fysal®, Orgacid® and Acidomix® were supplemented in the diets of chickens in treatments 2, 3 and 4 at 0.1% (1kg per ton/feed). Chickens fed diet supplemented  with Fysal® showed the best (P<0.05) results in total tract mineral retention 56.5+/-5.75 compared to the control and other treatments. The poor mineral retention i.e. 35 and 46% for calcium and phosphorus respectively by chickens fed the control diet was attributed to the insoluble phytate-mineral complex that limits mineral bioavailability. Intestinal segments of chickens fed acidified diets showed significant reduction (P<0.05) in pH in the range of 5.26-7.16 across duodenum to ileum compared to the control group 6.83-8.76. It is concluded that feed acidification at 0.1% improves mineral utilization in broiler chickens as it competes favourably with phytate in the lumen of the gut leading to better nutrient utilization.

scholarly journals Effect of Leaves Infuse (Foeniculum vulgare Mill.) Intake on Reproductive Organs Morphometry of Female Rats (Rattus Sp.) After Parturition

2017 ◽  
Vol 1 ◽  
pp. 27-30
Author(s):  
Najda Rifqiyati ◽  
Jumailatus Sholihah ◽  
Laelatul Soimah
Keyword(s):  
Reproductive Organs ◽  
Female Rats ◽  
Control Group ◽  
Foeniculum Vulgare ◽  
Treatment Groups ◽  
Randomized Design ◽  
Length Width ◽  
Completely Randomized Design ◽  
Layer Chromatography ◽  
Female Reproductive Organs

Fennel (Foeniculum vulgare Mill.) traditionally used by people as antifertility agent for female. Fennel leaves (Foeniculum vulgare Mill.) contain phytoestrogen that has role as antiestrogen or synthesized become antiestrogen. Coumarin is the kind phytoestrogen that can be antiestrogen to female reproductive organs such as ovary and utery. The aim of this research is to find out the influence of fennel leaves infuse (Foeniculum vulgare Mill.) on morphometric of ovary and utery in female rats (Rattus sp.) administered after parturition, to find out the effective dose  affecting the morphometric of ovary and utery and to find out the total coumarin consisted in fennel leaves (Foeniculum vulgare Mill.). This research was carried out by using CRD (Completely Randomized Design) consisted of 2 groups, those are control group (aquades) and treatment groups (doses of fennel leaves infuse 20 g/300 ml, 40 g/300 ml and 60 g/300 ml). The treatment was administered in the morning and afternoon for 15 days. After 15 days of treatment, the rats were sacrificed and dissected to measure the morphometry of reproductive organs (ovary and utery). The data obtained was analyzed by Analysis of Varian (ANOVA) and continued by LSD (Least Significance Different) test with significance of 5%. The measurement of coumarin level was performed by TLC (Thin Layer Chromatography) densitometry method. The result of this research showed that the administration of infused fennel leaves (Foeniculum vulgare Mill.) at dose of 20 g/300 ml, 40 g/300 ml and 60 g/300 ml significantly decreased ovarian weigh in female rats (Rattus sp.) after parturition (p<0,05), but not decreased the  length, width, thick of ovary, length and diameter of utery (p>0,05). No difference was observed between doses 20 g/300 ml, 40 g/300 ml and 60 g/300 ml, and it showed that the dose 20 g/300 ml of fennel leaves infuse was able to be used to decrease the weigth of ovary. Coumarin level observed in leaves is 0,09%, and it is higher than the coumarin content in fruit/seed.

scholarly journals Blood profile of growing rabbits fed roasted solvent extracted castor seed meal

2020 ◽  
Vol 44 (4) ◽  
pp. 160-166
Author(s):  
A. A. Ogunbode ◽  
O. S. Oladapo ◽  
O. A. Adeniyi ◽  
A. M. Oyetola
Keyword(s):  
Dietary Treatment ◽  
Blood Collection ◽  
Optimum Level ◽  
Feeding Trial ◽  
Blood Profile ◽  
Treatment Groups ◽  
Randomized Design ◽  
Collection Data ◽  
Completely Randomized Design ◽  
Dietary Treatments

 Sixteen growing rabbits with mean initial body weight of 750g were used in a feeding trial that lasted 56 days. The animals were randomly allotted into four (4) dietary treatment groups consisting of four (4) animals per group. Each treatment groups was replicated twice with individually housed rabbit per replicate.Thefourdietarytreatmentscontained0,0.5,1.0and 1.5% replacement levels of roasted-solvent extracted castor seedmeal (RSECSM) for soyabean in a completely randomized design experiment. The parameters investigated were haematological and serum indices. At the end of the feeding trial, blood samples were collected from the animals through the heart (left side) for haematology and serum indices. Eight (8) growing rabbits at the rate of two (2) animals per treatment were stunned and sacrificed for the blood collection. Data obtained were subjected to one way analysis of variance. The results show that feeding of RSECSM to growing rabbits did not show any significant (p>0.05) effecton PCV, Hb and RBC while the inclusion of RSECSM at graded dietary level had significant (p<0.05) effect on the WBC, WBC counts and Platelets. The WBC values ranged from 11025.0014900.00 while the values for platelets ranged from 72500.00 – 30500.00 respectively. Among the leukocyte differential counts examined lymphocytes, monocyte and eosinophil were significantly (p  <0.05) different among the dietary treatments, however neutrophil was not significantly (p >0.05) different among the means. The serum indices such as total protein, albumin, globulin, creatinine examined were not significantly(p >0.05) affected by the dietary treatment except the cholesterol. The results of this study indicated that 1.5% inclusion levels of RSECSM in the growing rabbit diet affect some haematological parameters while it appears to be the optimum level for serum indices.  

scholarly journals Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus

2019 ◽  
Vol 1 (2) ◽  
pp. 101-106
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Research Use ◽  
Treatment Groups ◽  
High Energy Milling ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the effect of histology of heart Mus musculus  after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the  Completely Randomized Design (CRD). Treatment consists of 6 groups, namely:  Control: CMC 0.5%; T1:100mg/20gBW; T2: 141,42mg/20gBW; T3:200mg/20gBW; T4: 282,82mg/20gBW;  T5:400mg/20gBW. The results of histology hearts that there were significant difference (P<0,05) between  control group and treatment groups but there was not significantly different in weight of hearts (P>0,05).

scholarly journals PENGARUH AIR REBUSAN DAUN HARENDONG BULU (Clidemia hirta) TERHADAP KADAR GULA DARAH MENCIT (Mus musculus)

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Okta Malia ◽  
Dian Samitra ◽  
Eka Lokaria
Keyword(s):  
Blood Sugar ◽  
Mus Musculus ◽  
Design Research ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Sugar Levels ◽  
Boiled Water ◽  
One Way Anova

This study aims to determine the effect of the water boiled leaves of Clidemia hirta on blood sugar levels in Swiss Webster mice (Mus musculus). This research using a completely randomized design research design (CRD) with 5 treatment groups namely; Aquades control group (P0), 3 g sucrose treatment (P1), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.13 ml/KgBB (P2), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.26 ml/KgBB (P3), 3 g treatment of sucrose + boiled bristle leaves boiled water at a dose of 0.39 ml/KgBB (P4). Before administration of the extract, mice in the treatment groups P1, P2, P3 & P4 were induced using 3 g of sucrose for 5 days. Then after 5 days for groups P2, P3, and P4 followed by giving boiled water leaves for 7 days. The analysis result of One Way Anova Test shows that Fcount > Ftable (18.86 > 2.51). This shows the boiled water affects the blood sugar levels of male Swiss Webster mice. The concentration of extract at P4 (0.39 ml/KgBB) was more effective in reducing blood sugar levels.

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