scholarly journals Keratin Hydrolysates Extracted from Sheep Wool with Potential Use as Organic Fertilizer

2020 ◽  
Vol 20 (3) ◽  
pp. 267-276
Author(s):  
Mariana Daniela Berechet ◽  
Demetra Simion ◽  
Maria Stanca ◽  
Cosmin-Andrei Alexe ◽  
Ciprian Chelaru ◽  
...  
Keyword(s):  
Amino Acids ◽  
Particle Size ◽  
Organic Fertilizer ◽  
Control Sample ◽  
Reaction Medium ◽  
Organic Fertilizers ◽  
Particle Measurements ◽  
Potential Use ◽  
Wheat Seeds ◽  
Sheep Wool

Keratin hydrolysates were obtained from sheep wool by alkaline hydrolysis at different concentrations of KOH (3%, 5% and 8%) and temperatures (75°C, 85°C, 95°C and 99°C) of the reaction medium. The protein content of the keratin extracts was between 65.54% and 87.10%. Particle measurements showed a decrease in particle size with the increase of concentration and temperature of the reaction medium. The ATR-FTIR spectra revealed specific bands to proteins and sulfur originated from keratin amino acids. The keratin hydrolysate type KerK895 was further investigated as organic fertilizer for two types of wheat seeds. The results showed that the use of 5% KerK895 led to the increase of the wheat stems lengths by 10.7% for Mirastar wheat and 18.3% for Tamino wheat, respectively, compared to control sample. Keratin hydrolysates are promising biopolymers as organic fertilizers in agriculture applications.

scholarly journals Effect of ecophysiological characteristics of tomato (Lycopersicon esculentum L.) in response to organic fertilizers (compost and vermicompost)

2020 ◽  
Vol 48 (3) ◽  
pp. 1248-1259
Author(s):  
Raheleh AHMADPOUR ◽  
Nezam ARMAND
Keyword(s):  
Organic Fertilizer ◽  
Control Sample ◽  
Dry Weight ◽  
Nutrient Content ◽  
High Water ◽  
Organic Fertilizers ◽  
Cell Membrane Stability ◽  
Tomato Seedlings ◽  
Randomized Design ◽  
Relative Water

The organic fertilizers such as urban wet-waste compost and vermicompost can improve the physicochemical properties of soil and have a favourite effect on plants growth due to the high nutrient content, high water holding capacity, plant growth regulators, and beneficial microorganisms. In this regard, this study was conducted to evaluate the organic fertilizer mixture on the physiological and morphological indices of tomato seedlings under greenhouse conditions. The experiment was done as factorial in a completely randomized design with 3 replications. The evaluated factors included the vermicompost fertilizer (0, 10, 20, 30 wt %), and the urban wet-waste compost (0, 10, 20, 30 wt %). In evaluation of the simple-effects of vermicompost and urban wet-waste compost the results showed that the 30 wt % level compared to the control sample increased the leaf area (+12.28% and +9.33%). It also increased the number of leaves (+17.5% and +22.9%), dry weight of root (+17.3% and +16.9%), chlorophyll-b content (+4.9% and +12.3 %), carotenoids (+2.9% and +7.9 %), and the total chlorophyll content (+23.7% and +13.8%). Results of evaluating the treatments showed that the vermicompost and urban wet-waste compost mixture in 30 wt % level (V4C4) caused significant increase in the plant height, leaf dry weight, root length, relative water content, cell membrane stability coefficient, efficiency of photochemical performance of PSII and the chlorophyll-a content (compared to other treatments especially low levels of organic fertilizer consumption). According to the final results of this study, using the vermicompost and urban wet waste compost mixture in 30 wt % level is recommended to improve the morphological and physiological traits of tomato in greenhouse conditions.

scholarly journals The effect of proteolytic enzymes on the amino acid composition of the filtrate and the grain mash obtained from the shock activator disintegrating treated rye

2019 ◽  
Vol 81 (1) ◽  
pp. 99-104
Author(s):  
A. A. Sabirov ◽  
N. V. Barakova ◽  
E. A. Samodelkin
Keyword(s):  
Amino Acids ◽  
Particle Size ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Proteolytic Enzymes ◽  
Average Particle Size ◽  
Control Sample ◽  
Amino Nitrogen ◽  
Average Particle

The effect of enzymes of proteolytic action on the amino acid composition of the filtrate and grains of grain wort obtained from UDA-treated rye was studied. The feasibility of using a complex of proteolytic enzymes: neutral and acid proteases is shown. The processing of rye was carried out on the disintegrator DEZI15 with different working elements: three and five-row rotors rotating at a frequency of 120 s1. A control sample of grinding was obtained in a laboratory mill with a rotor-knife working body. According to the data obtained on the Malvern Mastersizer 2000 laser analyzer, the average particle size in the grinding samples was: on a disintegrator with a three-row rotor – 167.5 microns, on a five-row rotor-knife working body – 384.278 microns. As a source of proteolytic enzymes, preparations of the fungal protease Рrо100L and the bacterial protease Protosubtilin GZх A120 were used. The determination of the content of ?-amino nitrogen in the finished wort (mg / dm3) was performed by calorimetry with ninhydrin. The concentration of amino acids was determined using the KNAUER amino acid analyzer: the calculation of aminograms was carried out by comparing the areas of the standard and the sample. It was established that the UDA-treatment allows obtaining a high content of ?-amino nitrogen in the filtrate of rye hydrolysates, and the complex introduction of acidic and neural proteases strengthens this effect. Analysis of the amino acid composition of the filtrate and grains showed the presence of all the essential amino acids in both of these products. The filtrate can be recommended as a nutrient nitrogen-containing medium for the cultivation of microorganisms, followed by the release of secondary metabolites of their vital activity, or as an intermediate in the production of sugar-containing syrups from whole grains with a high content of amino acids. The fraction of the hydrolyzate is of interest as a protein-containing feed additive, and the fine particle size distribution of such a product will allow it to be used not only for feeding cattle, but also in poultry farming.

Chemical Transformation of Fe, Air & Water to Ammonia: Variation of Reaction Rate with Temperature, Pressure, Alkalinity and Iron

2018 ◽  
Author(s):  
Ping Peng ◽  
Fang-Fang Li ◽  
Xinye Liu ◽  
Jiawen Ren ◽  
jessica stuart ◽  
...  
Keyword(s):  
Particle Size ◽  
Reaction Rate ◽  
Electrolyte Concentration ◽  
Iron Concentration ◽  
Reaction Medium ◽  
Iron Particle ◽  
Intermediate Step ◽  
Ammonia Production ◽  
Pure Nitrogen ◽  
Alkaline Reaction

The rate of ammonia production by the <u>chemical </u>oxidation of iron, N<sub>2</sub>(from air or as pure nitrogen) and water is studied as a function of (1) iron particle size, (2) iron concentration, (3) temperature, (4) pressureand (5) concentration of the alkaline reaction medium. The reaction meduium consists of an aqueous solution of equal molal concentrations of NaOH and KOH (Na<sub>0.5</sub>K<sub>0.5</sub>OH). We had previously reported on the <u>chemical </u>reaction of iron and nitrogen in alkaline medium to ammonia as an intermediate step in the <u>electrochemical </u>synthesis of ammonia by a nano-sized iron oxide electrocatlyst. Here, the intermediate <u>chemical </u>reaction step is exclusively explored. The ammonia production rate increases with temperature (from 20 to 250°C), pressure (from 1 atm to 15 atm of air or N<sub>2</sub>), and exhibits a maximum rate at an electrolyte concentration of 8 molal Na<sub>0,5</sub>K<sub>0,5</sub>OH in a sealed N<sub>2</sub>reactor. 1-3 µm particle size Fe drive the highest observed ammonia production reaction rate. The Fe mass normalized rate of ammonia production increases with decreasing added mass of the Fe reactant reaching a maximum observed rate of 2.2x10<sup>-4</sup>mole of NH<sub>3</sub>h<sup>-1</sup>g<sup>-1</sup>for the reaction of 0.1 g of 1-3 µm Fe in 200°C 8 molal Na<sub>0.5</sub>K<sub>0.5</sub>OH at 15 atm. Under these conditions 5.1 wt% of the iron reacts to form NH<sub>3</sub>via the reaction N<sub>2</sub>+ 2Fe + 3H<sub>2</sub>O ®2NH<sub>3</sub>+ Fe<sub>2</sub>O<sub>3</sub>.

scholarly journals First reported quantitative microbiota in different livestock manures used as organic fertilizers in the Northeast of Thailand

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lampet Wongsaroj ◽  
Ratmanee Chanabun ◽  
Naruemon Tunsakul ◽  
Pinidphon Prombutara ◽  
Somsak Panha ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Organic Fertilizer ◽  
The Other ◽  
Organic Fertilizers ◽  
Rrna Gene ◽  
Bacterial Composition ◽  
Physiochemical Properties ◽  
Nutrient Profiles ◽  
Soil Recovery ◽  
Local Farmers

AbstractNortheastern Thailand relies on agriculture as a major economic activity, and has used high levels of agrochemicals due to low facility, and salty sandy soil. To support soil recovery and sustainable agriculture, local farmers have used organic fertilizers from farmed animal feces. However, knowledge about these animal fecal manures remains minimal restricting their optimal use. Specifically, while bacteria are important for soil and plant growth, an abundance and a diversity of bacterial composition in these animal fecal manures have not been reported to allow selection and adjustment for a more effective organic fertilizer. This study thereby utilized metagenomics combined with 16S rRNA gene quantitative PCR (qPCR) and sequencing to analyze quantitative microbiota profiles in association with nutrients (N, P, K), organic matters, and the other physiochemical properties, of the commonly used earthworm manure and other manures from livestock animals (including breed and feeding diet variations) in the region. Unlike the other manures, the earthworm manure demonstrated more favorable nutrient profiles and physiochemical properties for forming fertile soil. Despite low total microbial biomass, the microbiota were enriched with maximal OTUs and Chao richness, and no plant pathogenic bacteria were found based on the VFDB database. The microbial metabolic potentials supported functions to promote crop growth, such as C, N and P cyclings, xenobiotic degradation, and synthesis of bioactive compounds. Pearson’s correlation analyses indicated that the quantitative microbiota of the earthworm manure were clustered in the same direction as N, and conductivity, salinity, and water content were essential to control the microbiota of animal manures.

scholarly journals Conversion of Spent Coffee and Donuts by Black Soldier Fly (Hermetia illucens) Larvae into Potential Resources for Animal and Plant Farming

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 332
Author(s):  
Hayden Fischer ◽  
Nicholas Romano ◽  
Amit Kumar Sinha
Keyword(s):  
Organic Waste ◽  
Glycogen Content ◽  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Hermetia Illucens Larvae ◽  
Nitrogen Phosphorus ◽  
Dough Stage ◽  
Plant Farming

Nutritionally unbalanced organic waste can be converted into potential resources for animal and plant farming by culturing black soldier fly (Hermetia illucens) larvae (BSFL) and prepupae (BSFP). BSFL and BSFP are rich sources of protein and lipids, while the leftover excrement called “frass” can be used as an organic fertilizer. Using readily available resources, BSFL were cultured on spent coffee, donut dough or an equal blend for 35 days. Survival, productivity, daily pupation and biochemical composition of BSFL and BSFP were measured along with the nitrogen-phosphorus-potassium values of the frass. Survival was highest in the blend compared (81%) to spent coffee (45%) or dough (24%); however, BSFL and BSFP were significantly longer and heavier from dough. Stage and food significantly influenced the protein, lipid and glycogen content of the BSFL and BSFP, which tended to be higher in the latter. While fatty acids were often significantly higher in BSFL fed spent coffee, the amino acid composition of BSFL was generally higher in dough. Frass from the blend had significantly highest nitrogen content, while potassium and phosphorus were significantly higher and lower from spent coffee, respectively. Although coffee and donut dough were suboptimal substrates for BSFL, a blend of these produced BSFL and frass that were nutritionally comparable to soybean meal and many organic fertilizers, respectively.

The Use of Rheology for Sludge Characterization

1982 ◽  
Vol 14 (6-7) ◽  
pp. 475-489 ◽  
Author(s):  
H W Campbell ◽  
P J Crescuolo
Keyword(s):  
Particle Size ◽  
Sewage Sludge ◽  
Test Procedures ◽  
Particle Size Reduction ◽  
Rotational Viscometer ◽  
Polymer Addition ◽  
Rheological Measurements ◽  
Acceleration Rate ◽  
Sludge Characterization ◽  
Potential Use

Rheological measurements were conducted on a variety of anaerobically digested sewage sludges to evaluate the potential use of rheology in describing the effects of chemical conditioning on the physical characteristics of sludges. The objectives of the study were to evaluate the influence of the method of chemical conditioning on rheological measurements; to determine the response of the viscometer system to changes in the instrument variables; and to evaluate interrelationships between rheology and other physical properties. All rheological measurements were made using a coaxial rotational viscometer. Evaluation of a variety of test procedures identified that both the method of adding chemical conditioners, and the acceleration rate of the rotational viscometer, could significantly alter the shape of the rheograms. A suggested methodology was identified and selected samples were analyzed in triplicate to test the reproducibility of the procedures. Existing mathematical models do not adequately describe the variety of flow behaviour patterns observed with sewage sludge. The concepts of yield stress and apparent viscosity also have limited value due to problems of definition and calculation. A parameter termed the “instantaneous viscosity”, defined as the derivative of the flow curve, is suggested as being more suitable for describing sludge behaviour. The relationships between chemical conditioning, particle size distribution and applied shear were explored. As polymer addition increased, the sludge particles became more susceptible to shear breakup. The extent of particle size reduction was a function of the rate of shear and the time during which the shear was maintained.

The effect of nursery substrate and fertilization on the growth and ectomycorrhizal status of containerized and outplanted seedlings of Picea abies

2009 ◽  
Vol 39 (1) ◽  
pp. 64-75 ◽  
Author(s):  
Lu-Min Vaario ◽  
Arja Tervonen ◽  
Kati Haukioja ◽  
Markku Haukioja ◽  
Taina Pennanen ◽  
...  
Keyword(s):  
Growth Rate ◽  
Picea Abies ◽  
Norway Spruce ◽  
Growth And Development ◽  
Organic Fertilizer ◽  
Wood Fibre ◽  
Organic Fertilizers ◽  
Relative Growth ◽  
Ecm Fungi ◽  
Influence Of Substrate

Over a 5 year period, we examined the influence of substrate and fertilization on nursery growth and outplanting performance of Norway spruce (Picea abies (L.) Karst.). We focused on the relative growth and development of roots and shoots and the colonization intensity and diversity of ectomycorrhizal (ECM) fungi. In the nursery, a conventional substrate (low-humified Sphagnum peat) supplemented with woody material (wood fibre and pine bark) and either mineral or organic fertilizers yielded shorter seedlings than those grown on the unmodified substrate. However, after outplanting, the growth rate of seedlings cultivated on modified substrates was higher than that of seedlings grown on the unmodified substrate. Seedlings cultivated in modified substrates had significantly higher root/shoot ratios and ECM diversity; the latter remained significant after ≥3 years of outplanting. Seedlings grown on a substrate containing 50% woody material and supplemented with organic fertilizer had the highest growth rate among all seedlings during the 3 year period of outplanting. Colonization intensity of ECM fungi was high in all seedlings except for those grown in heavily fertilized substrate. This study suggests that nursery techniques that produce seedlings with higher root/shoot ratios and ECM diversities could improve plantation success and growth rate for at least the first 3 years of outplanting.

Effects of Reaction Medium on the Phase Synthesis and Particle Size Evolution of BaTiO3

2010 ◽  
Vol 93 (10) ◽  
pp. 3443-3448 ◽  
Author(s):  
Tomasz M. Stawski ◽  
Sjoerd A. Veldhuis ◽  
Ole F. Göbel ◽  
Johan E. Ten Elshof ◽  
Dave H. A. Blank
Keyword(s):  
Particle Size ◽  
Reaction Medium ◽  
Phase Synthesis ◽  
Size Evolution

Responses of natural 15N abundance in cauliflower (Brassica oleracea L. var. botrytis) and soil to the application of organic and chemical fertilizers

2016 ◽  
Vol 96 (5) ◽  
pp. 819-827 ◽  
Author(s):  
Hui Wang ◽  
Guoqing Hu ◽  
Yanhong Lou ◽  
Zhihui Su ◽  
Yuping Zhuge ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Plant Root ◽  
Organic Fertilizer ◽  
Nitrogen Isotope ◽  
Organic Production ◽  
Organic Fertilizers ◽  
Chemical Fertilizers ◽  
Plant Parts ◽  
Brassica Oleracea L ◽  
15N Abundance

Natural nitrogen isotope abundance (δ15N) has been suggested as a potential indicator for discriminating organic vegetables from their conventional counterparts. However, little is known about the δ15N characteristic of cauliflower (Brassica oleracea L. var. botrytis) under organic production and how the application of chemical fertilizers could affect this characteristic. In this study, δ15N characteristics of cauliflower (including the intra-plant δ15N characteristic) and soil under different fertilization treatments were investigated under field conditions. Five treatments were laid out: control, chemical fertilizers (F4M0), organic fertilizer (F0M4), and chemical fertilizer in combination with organic fertilizer at the ratio of 3:1 (F3M1) and 1:3 (F1M3). Results showed that plant δ15N was positively correlated with fertilizer δ15N and plant root/flower was more sensitive to changes of fertilizer δ15N and soil δ15N than leaf/stem. Distribution of δ15N in different plant parts (especially in flower and leaf) was significantly changed when organic fertilizers were partially or totally substituted by chemical fertilizers, suggesting a comparison of flower δ15N and leaf δ15N might be applicable for the organic identification of marketed cauliflowers. Our results provide fundamental data for the soil- and fertilizer-specific crop δ15N database and indicate that the intra-plant δ15N characteristic could be helpful for organic cauliflower identification.

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