scholarly journals FCγII (CD32) MONOSIT DI INFEKSI DENGUE PRIMER DAN SEKUNDER {FcγRII (CD32) Monocytes in Primary and Secondary Dengue Infection}

2018 ◽  
Vol 22 (1) ◽  
pp. 42
Author(s):  
Umi S. Intansari ◽  
Usi Sukorini ◽  
Shanti Ika Sari
Keyword(s):  
Neutralizing Antibodies ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Density Ratio ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Cross Sectional ◽  
Major Health Problem ◽  
Secondary Infections ◽  
Capture Method

Dengue infection is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection varywidely, from asymptomatic until dengue shock syndrome (DSS). Antibody Dependent Enhancement (ADE) hypothesis that states thatnon-neutralizing antibodies in secondary dengue infection may enhance dengue infection via Fcγ receptors is still controversial. Clinicalresearch shows that not all secondary infections manifest as DHF/DSS, but nearly all DHF/DSS cases are caused by secondary infection.Allegedly, the expression of Fcγ has an effect on this incident. This study is an observational analytical study with a cross sectional designto determine the expression of FcγRII (CD32) monocytes in patients with primary and secondary dengue infection. CD32 of monocyteswas measured using FACS Calibur with lyse no wash technique. Primary and secondary dengue infection were determined by IgM/IgGoptical density ratio using ELISA capture method. The ratio of IgM/IgG ≥1.2 was considered as primary infection, while the ratio <1.2was considered as secondary infection. Twenty primary and 32 secondary dengue infection patients in acute phase of dengue infectionpartisipated in this study. Expressions of Fcγ RII (CD32) monocytes were significantly lower in primary than secondary dengue infection(187.825±31.584 vs 218.598±43.414 MFI; p=0.008). CD32 expressions were higher in day 3 compared to day 4 of fever.

scholarly journals MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN

2020 ◽  
Vol 8 (1) ◽  
pp. 30
Author(s):  
Indah Agustiningrum ◽  
Jusak Nugraha ◽  
Hartono Kahar
Keyword(s):  
Healthy Subjects ◽  
Vascular Endothelial Cells ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Structural Protein ◽  
Cross Sectional ◽  
Major Health Problem ◽  
Cytokines And Chemokines ◽  
Significant Difference

Dengue infection caused by DENV and transmitted by mosquitoes Aedes aegypti and Aedes albopictus is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection are very widely, from asymptomatic until dengue shock syndrome (DSS). DENV will attack macrophages and dendritic cells (DC) and replicate them. Monocytes are macrophages in the blood (±10% leukocytes). Macrophages produce cytokines and chemokines such as monocyte chemotactic protein-1 (MCP-1)/CCL2. The monocytes that are infected with DENV will express MCP-1, which will increase the permeability of vascular endothelial cells so that they have a risk of developing DHF/DSS. Macrophages and DC secrete NS1 proteins, which are the co-factors that are needed for viral replication and can be detected in the early phase of fever. The increased MCP-1 levels in dengue infection followed by an increase in the number of atypical lymphocytes indicate the arrival of macrophages and monocytes to the site of inflammation which triggers proliferation rather than lymphocytes. This is an observational analytical study with a cross-sectional design to determine the MCP-1 level in dengue infection patients with 1st until the 4th day of fever and the presence of atypical lymphocytes. Dengue infection was determined by rapid tests NS1 positive or negative and MCP-1 levels were measured using by ELISA sandwich method.MCP-1 level of sixty patients dengue infection NS-1 rapid positive or negative with 2nd until 4rt fever were significantly higher than healthy subjects (420.263±158,496vs29, 475±23.443;p=0.000), but there was no significant difference in subjects with DF, DHF or DSS (436,47±225,59 vs422,77±170,55vs 448,50±117,39; p =0.844). Atypicallymphosite differs significantly in healthy  subjects than subjects infected with DENV an average of 2% (p= 0,000). In conclusion, this shows the arrival of macrophages and monocytes to the site of inflammation, which triggers the proliferation of lymphocytes.

scholarly journals Perbandingan Tingkat Keparahan Infeksi Sekunder Virus Dengue pada Keempat Serotipe di Indonesia: Systematic Review

2021 ◽  
Vol 10 (1) ◽  
pp. 49
Author(s):  
Annelin Kurniati ◽  
Ahmad Fandi ◽  
Mardhatillah Sariyanti ◽  
Ety Febrianti ◽  
Debie Rizqoh
Keyword(s):  
Dengue Virus ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Severity Of Illness ◽  
Dengue Shock Syndrome ◽  
Severe Dengue ◽  
Literature Study ◽  
Secondary Infections ◽  
Virus Serotype ◽  
Dengue Virus Serotypes

Secondary infection with the dengue virus causes mild to severe manifestations. The distribution of dengue virus serotypes varies in various areas and can change over time. There are four dengue serotypes, namely DENV-1, DENV-2, DENV-3 and DENV-4. Objectives: To knew the distribution of virus serotypes in an area and determined the pathogenesis of the disease, which can cause severe manifestations in patients with secondary infections. Methods: The data taken is the severity of secondary infections and dengue serotypes. The literature search was performed on PMC and Cochrane. Search criteria were performed using keywords (secondary infection * OR secondary dengue infection *) AND (Dengue Virus * OR Dengue Infection * OR Dengue * OR DENV) AND (Serotype * OR Serogroup) AND (severe dengue * OR severity * OR severity of illness indexs * OR dengue fever * OR dengue haemorrhage fever * OR dengue shock syndrome * OR DF * OR DHF * OR DSS *) AND (Indonesia *). Results: Literature study search found 387 literature with five studies conducted the analysis. From the results of the analysis, it was found that secondary infections were more common in patients with recurrent dengue infection with serotype 2 (DENV-2), serotype 3 (DENV-3) and serotype 4 (DENV-4). Conclusion: Secondary infection of dengue virus serotype 2 (DENV-2) and serotype 3 (DENV-3) can cause severe dengue infection.Keywords:  Dengue Virus, Indonesia, Secondary Infection, Serotype, Severity

scholarly journals Epidemiological and Clinical Features of Dengue Infection in Adults in the 2017 Outbreak in Vietnam

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Bui Vu Huy ◽  
Le Nguyen Minh Hoa ◽  
Dang Thi Thuy ◽  
Nguyen Van Kinh ◽  
Ta Thi Dieu Ngan ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Dengue Fever ◽  
Clinical Features ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Warning Signs ◽  
Severe Dengue ◽  
Severe Bleeding ◽  
Cross Sectional ◽  
Number Of Patients

Purpose. The clinical features and laboratory results of dengue-infected adult patients admitted to the hospital during the 2017 outbreak were analyzed in this study. Method. This is a cross-sectional study. 2922 patients aged 18 years or more with dengue fever in National Hospital for Tropical Diseases (NHTD) in the North and Hospital for Tropical Disease (HTD) in the South of Vietnam were recruited in this study. Result. Patients were admitted in the hospital around the year and concentrated from August to December, in 53/63 (84.0%) provinces in Vietnam, and patients in all ages were affected. The number of patients with dengue fever was 1675 (57.3%), dengue with warning signs 914 (31.3%), and severe dengue 333 (11.4%), respectively. Among patients with severe dengue, severe plasma leakage and dengue shock account for 238 (8.1%), severe organ impairment 73 (2.5%), and severe bleeding 22 (0.75%). The rate of mortality was 0.8%, and the outcome of dengue patients is worse in the elderly and people with underlying diseases. Conclusion. The 2017 dengue outbreak occurred in a larger scale than in the previous years in terms of time, location, and number of patients. More elderly patients were infected by dengue in this outbreak, and this may contribute to the mortality rate. Clinical manifestations of dengue patients in Southern Vietnam are more typical than the northern, but the rate of severe dengue is not different. The mortality risk and underlying conditions associated with dengue-infected elderly patients are worthy of further investigations in the future.

Shock Prognostic Factors in Hospitalized Dengue Hemorrhagic Fever Children

2018 ◽  
Vol 24 (8) ◽  
pp. 6221-6224
Author(s):  
Hindra Irawan Satari ◽  
Rossy Agus Mardani ◽  
Hartono Gunardi
Keyword(s):  
Prognostic Factors ◽  
Dengue Hemorrhagic Fever ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Health Department ◽  
Abdominal Tenderness ◽  
Diverse Area ◽  
Plasma Leakage

Various clinical manifestations, complex pathogenesis and different virus serotypes in diverse area make us difficult to predict course of disease, even the child admitted in early. Prognostic factors are very important to predict cases progressing to become DSS. Dengue shock syndrome (DSS) occurs in 15.53% of Dengue hemorrhagic fever (DHF) patients with 7.81% mortality rate. Aim: To explore the prognostic factors of shock in hospitalized DHF children regarding the new 2011 WHO dengue virus infection classification guideline. This was a retrospective study using medical records of children age below 18 years old with WHO fulfilled grade 1 and 2 DHF diagnosis from January 2013–December 2016 in Child Health Department of Dr. Cipto Mangunkusumo Hospital, Jakarta. Independent variables were sex, age, nutritional status, secondary dengue infection, leucopenia, abdominal tenderness, gastrointestinal bleeding, hepatomegaly and plasma leakage. Shock was dependent variable. Multivariate analysis was done by using logistic regresion analysis. There were 98 DHF subjects, 5 subjects became DSS during hospitalization. DSS subject characteristics were age of >5 years old, female, malnutrition, abdominal tenderness, hemoconcentration ≥20%, secondary dengue infection, leucocyte ≥5.000 mm3 and thrombocyte <50.000 mm3. Multivaryate analysis showed malnutrition and hepatomegaly were prognosis factors of DSS. Malnutrition and hepatomegaly were prognotic factors of dengue shock syndrome. Clinicians should be more aware with these factors when managed hospitalized DHF patients.

scholarly journals Effect of Prior Symptomatic Dengue Infection on Dengue Haemorrhagic Fever (DHF) in Children

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Randula Ranawaka ◽  
Chamara Jayamanne ◽  
Kavinda Dayasiri ◽  
Dinuka Samaranayake ◽  
Udara Sandakelum ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Past History ◽  
Secondary Infection ◽  
Severe Disease ◽  
Dengue Infection ◽  
Dengue Shock Syndrome ◽  
Dengue Haemorrhagic Fever ◽  
Severe Dengue ◽  
Haemorrhagic Fever ◽  
History Of

Pathogenesis of dengue haemorrhagic fever is not fully understood, but it is thought that there is antibody enhancement during the secondary infection, which causes severe dengue haemorrhagic fever (DHF). Therefore, patients who have DHF should have a documented history of symptomatic dengue infection in the past. A retrospective descriptive-analytical study was conducted at the University Paediatric Unit at Lady Ridgeway Hospital for Children, Colombo, Sri Lanka. All children who had fulfilled the criteria for DHF admitted to the unit from April 2018 to September 2018 were recruited into the study. Relevant data were collected from bed head tickets. One hundred and eighty-four children were included in the final analysis. Thirty-three (17.9%) had a past history of documented symptomatic dengue infection, while 82.1% did not have a documented dengue infection. Twelve patients had dengue shock syndrome, and none of them had previously documented symptomatic dengue fever. Dextran was used in 96 patients in the critical phase. Twelve (42%) patients with past documented symptomatic dengue fever needed dextran while 84 (54.9%) patients without a documented past history of dengue fever needed dextran. In our clinical observation, we noticed that children with DHF mostly did not have a documented symptomatic prior dengue infection, while those with a documented symptomatic prior infection had a milder subsequent illness. In fact, the majority (82.1%) of patients with DHF did not have documented previous symptomatic dengue infection. It was also observed that the clinical course of subsequent dengue infection was less severe in patients with previously documented symptomatic dengue fever. This finding should be further evaluated in a larger scale study minimizing the all-confounding factors. This fact is more important in selecting recipients for vaccines against the dengue virus, which are supposed to produce immunity against the virus without causing the severe disease.

scholarly journals Dengue Immunopathogenesis: A Crosstalk between Host and Viral Factors Leading to Disease: Part I - Dengue Virus Tropism, Host Innate Immune Responses, and Subversion of Antiviral Responses

2020 ◽  
Author(s):  
Henry Puerta-Guardo ◽  
Scott B. Biering ◽  
Eva Harris ◽  
Norma Pavia-Ruz ◽  
Gonzalo Vázquez-Prokopec ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Nonstructural Protein ◽  
Secondary Infection ◽  
Hypovolemic Shock ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Innate Immune ◽  
Vascular Leak ◽  
Dengue Disease

Dengue is the most prevalent emerging mosquito-borne viral disease, affecting more than 40% of the human population worldwide. Many symptomatic dengue virus (DENV) infections result in a relatively benign disease course known as dengue fever (DF). However, a small proportion of patients develop severe clinical manifestations, englobed in two main categories known as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Secondary infection with any of the four dengue virus serotypes (DENV1, -2, -3, and -4) is a risk factor to develop severe forms of dengue disease. DSS is primarily characterized by sudden and abrupt endothelial dysfunction, resulting in vascular leak and organ impairment, which may progress to hypovolemic shock and death. Severe DENV disease (DHF/DSS) is thought to follow a complex relationship between distinct immunopathogenic processes involving host and viral factors, such as the serotype cross-reactive antibody-dependent enhancement (ADE), the activation of T cells and complement pathways, the phenomenon of the cytokine storm, and the newly described viral toxin activity of the nonstructural protein 1 (NS1), which together play critical roles in inducing vascular leak and virus pathogenesis. In this chapter that is divided in two parts, we will outline the recent advances in our understanding of DENV pathogenesis, highlighting key viral-host interactions and discussing how these interactions may contribute to DENV immunopathology and the development of vascular leak, a hallmark of severe dengue. Part I will address the general features of the DENV complex, including the virus structure and genome, epidemiology, and clinical outcomes, followed by an updated review of the literature describing the host innate immune strategies as well as the viral mechanisms acting against and in favor of the DENV replication cycle and infection.

scholarly journals Interpretation of the presence of IgM and IgG antibodies in a rapid test for dengue: analysis of dengue antibody prevalence in Fortaleza City in the 20th year of the epidemic

2012 ◽  
Vol 45 (2) ◽  
pp. 163-167 ◽  
Author(s):  
José Rubens Costa Lima ◽  
Maria Zélia Rouquayrol ◽  
Maria Roseli Monteiro Callado ◽  
Maria Izabel Florindo Guedes ◽  
Claudia Pessoa
Keyword(s):  
Attack Rate ◽  
Dengue Infection ◽  
Population Sample ◽  
Rapid Test ◽  
Laboratory Diagnosis ◽  
Epidemiological Studies ◽  
Igg Antibodies ◽  
Cross Sectional ◽  
Secondary Infections ◽  
Main Technique

INTRODUCTION: The diagnosis of dengue and the differentiation between primary and secondary infections are important for monitoring the spread of the epidemic and identifying the risk of severe forms of the disease. The detection of immunoglobulin (Ig)M and IgG antibodies is the main technique for the laboratory diagnosis of dengue. The present study assessed the application of a rapid test for dengue concerning detection of new cases, reinfection recognition, and estimation of the epidemic attack rate. METHODS: This was a retrospective, cross-sectional, descriptive study on dengue using the Fortaleza Health Municipal Department database. The results from 1,530 tested samples, from 2005-2006, were compared with data from epidemiological studies of dengue outbreaks in 1996, 2003, and 2010. RESULTS: The rapid test confirmed 52% recent infections in the tested patients with clinical suspicion of dengue: 40% detected using IgM and 12% of new cases using IgG in the non-reactive IgM results. The positive IgM plus negative IgG (IgM+ plus IgG-) results showed that 38% of those patients had a recent primary dengue infection, while the positive IgG plus either positive or negative IgM (IgG+ plus IgM+/-) results indicated that 62% had dengue for at least a second time (recent secondary infections). This proportion of reinfections permitted us to estimate the attack rate as >62% of the population sample. CONCLUSIONS: The rapid test for dengue has enhanced our ability to detect new infections and to characterize them into primary and secondary infections, permitting the estimation of the minimal attack rate for a population during an outbreak.

scholarly journals COVID-19 with dengue shock syndrome in a child: coinfection or cross-reactivity?

2020 ◽  
Vol 13 (12) ◽  
pp. e239315
Author(s):  
Lokesh Tiwari ◽  
Shashank Shekhar ◽  
Anmol Bansal ◽  
Pradeep Kumar
Keyword(s):  
Dengue Infection ◽  
Management Strategies ◽  
Clinical Manifestations ◽  
International Health ◽  
Dengue Shock Syndrome ◽  
Cross Reactivity ◽  
Clinical Syndrome ◽  
Unique Challenge ◽  
Endemic Diseases ◽  
Paediatric Case

COVID-19, caused by SARS-CoV-2, has spread globally. Coinfection with other endemic viruses is likely to complicate the clinical presentation and outcome. Information on clinical manifestations and management strategies on COVID-19 coinfection with endemic diseases in children is yet to evolve. The risk of dengue infection exists in 129 countries and it is endemic in more than 100 countries. The SARS-CoV-2 pandemic might overlap with the dengue epidemics in tropical countries. We report the first paediatric case to the best of our knowledge of COVID-19 encephalitis with dengue shock syndrome. This clinical syndrome could be attributed to serological cross-reactivity, incidental coinfection or perhaps a warning for dengue-endemic regions to face the unique challenge of differentiating and managing two disease entities together. Enhanced understanding of potential COVID-19 and dengue coinfection warrants immediate attention of researchers and international health policy makers.

scholarly journals Dengue Lurks During Coronavirus Disease-19 Pandemic in Indonesia: A Narrative Review

2020 ◽  
Vol 8 (T1) ◽  
pp. 391-398
Author(s):  
Wienta Diarsvitri ◽  
Retno Budiarti ◽  
Pramita Anindya Nugraheni ◽  
M. Fathi Ilmawan ◽  
Verna Biutifasari
Keyword(s):  
Secondary Infection ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Clinical Pathology ◽  
Organ Damage ◽  
Careful Consideration ◽  
Narrative Review ◽  
Antigenic Structure ◽  
Serological Tests ◽  
Laboratory Findings

BACKGROUND: Most attention and healthcare resources in Indonesia have been geared toward battling the coronavirus (CoV) disease (COVID)-19 pandemic, and less overture has been given to the looming risks of dengue that has been endemic in many areas of Indonesia. Despite related constraints, the Primary Health Cares (PHC) in Indonesia plays an important role in the face of emergency situations. AIM: This study aimed to review the dengue and COVID-19 infection, clinical manifestations in children and adults, clinical pathology findings, as well as the prevention strategies that could be applied in PHC. METHODS: This study is a narrative review based on the research articles and reports that were published between 2010 and 2020. A total of 70 articles and reports were obtained and after careful consideration, 58 articles and reports were used as references of this study. RESULTS: Both dengue virus (DENV) and severe acute respiratory syndrome-CoV (SARS-CoV-2) share the similarity of antigenic structure, common symptoms, and laboratory findings. The immune response in SARS-CoV-2 may cause a cytokine storm, which can increase vascular permeability and organ damage. Secondary infection of DENV with different strains may allow the occurrence of antibody-dependent enhancement. The cross-reactions between SARS-CoV-2 antibodies and DENV antigens may cause false positive on rapid dengue infection serological tests. CONCLUSION: PHC as the front line of health services has a fundamental role in the crisis situation. The prevention and control of DENV and SARS-CoV-2 infections are based on the mode of transmission and need compliance to the related health protocols.

scholarly journals Serum Cytokine Profiles in Patients with Dengue Fever at the Acute Infection Phase

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Junyuan Huang ◽  
Weiwen Liang ◽  
Shaoyan Chen ◽  
Ye Zhu ◽  
Haiming Chen ◽  
...  
Keyword(s):  
Secondary Infection ◽  
Severe Disease ◽  
Acute Infection ◽  
Dengue Infection ◽  
Acute Stage ◽  
Control Group ◽  
Disease Symptoms ◽  
Guangzhou City ◽  
Cytokine Profiles ◽  
Secondary Infections

Background. Dengue virus (DENV) is transmitted by mosquito and has been circulating in Guangdong, China, for over 30 years. Dengue infection causes mild to severe disease symptoms in human. Cytokine profiles were suggested to be crucial especially during the acute stage in the dengue infection. Aim. To determine the cytokine profiles at the acute stage in patients with primary or secondary dengue infection in Guangzhou city in the 2014 outbreak. Methods. We investigated 23 inflammatory cytokines in serum collected from dengue-infected patients and analyzed their correlations with their clinical indexes. Results. The concentrations of CXCL9, IP-10, CXCL11, IL-8, IL-10, and CCL2 in serum were significantly higher in the groups of DENV-infected patients during the first two weeks than those of control group while CCL17 and CXCL5 showed lower expression level in the patients. Among these cytokines, CXCL9, CCL17, and CXCL5 showed statistical difference between the groups of primary and secondary infections. The platelet count and lactate dehydrogenase were correlated with the level of CCL17 and MIP-1α/CXCL5, respectively, in the group of secondary infection. Conclusions. We determined the cytokine profiles in serum of the patients during the 2014 dengue outbreak. The expression of specific cytokines was associated with the secondary infection.

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