I shall not attempt to summarize the content of today’s papers and discussions. We have, as expected, travelled from molecules to mammals, and from physics to physiology. We have considered much of the available information about the changes which occur when a living cell freezes and how these changes may be destructive to the cell. The removal of water by freezing sets up a variety of stresses and strains in or on the cell. These stresses may be mechanical, biophysical or biochemical in nature; it is difficult at present to assign an exact role to each. What is clear is that knowledge in this field is in its infancy and that factors hitherto unsuspected may play a major part. The likelihood of ‘bubble trouble’ in thawed tissues, discussed today, is a good example of a hitherto unsuspected complication arising from the temporary removal of water. The extent of the direct effect of cold, as such, on the biochemical components of the cell is even less certain, and it may well be that the destructive effects on the lipoproteins are accompanied by equal damage to the enzymic and other active systems. The capacity of various neutral solutes, notably glycerol, to decrease or even prevent the damage caused by freezing and thawing has featured largely in today’s proceedings and here even more problems appear. It seems that according to the type of cell, the glycerol can be applied abruptly and the cell returned to a normal medium abruptly, or it can be applied abruptly, but must be removed gradually to avoid osmotic damage to the cell, or it must be both applied and removed slowly. There seems little doubt that the glycerol passes to the interior of the cell, and some evidence that it must do so to be effective. The optimal concentration of the substance evidently varies according to the type of cell, condition of freezing, nature of the suspending medium and so on. Having satisfactorily applied glycerol, freezing can vary according to the temperature gradient and the terminal temperature. Today’s discussions have made it clear that we are only at the beginning of the study of the effect of cooling velocity on viability of the cells.