scholarly journals Fenugreek seeds estrogenic activity in ovariectomized female rats

2019 ◽  
Vol 32 (3) ◽  
pp. 138-145
Author(s):  
Hind Brogi ◽  
Hajji Elbachir ◽  
Najat El Amrani ◽  
Siham Amsaguine ◽  
Driss Radallah
Keyword(s):  
Estrogenic Activity ◽  
Progesterone Receptors ◽  
Cell Number ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Fenugreek Seeds ◽  
Estrogenic Potential ◽  
Estrogenic Action ◽  
Traditional Pharmacopoeia ◽  
Trigonella Foenum Graecum L

Abstract The estrogenic activities of fenugreek seeds (Trigonella foenum-graecum L.), widely used in traditional pharmacopoeia, are reflected in the uterus of ovariectomized female rats, with a slight increase in dry and wet weight, a thickening of the stroma and the uterine epithelium and the development of the endometrial glands. In the vagina, the estrogenic action is shown through an increase in the epidermal cell number and a tendency to keratinization, leading to vaginal opening. Furthermore, this estrogenic potential of fenugreek seeds is confirmed by the over-expression of progesterone receptors in the uterine tissues supporting possible interactions between phytoestrogens and estrogen receptors. Therefore, Fenugreek seeds may be capable of promoting the development of reproductive tissues of immature ovariectomized rats, and its estrogenic activity may take its action by holding phystoestrogens that interact with estrogen binding sites and activate the same estradiol-mediated cell signaling pathways. Thus, our results give added scientific support to the popular use of Fenugreek seeds as an alternative for several health problems such as fertility and menopause related disorders.

scholarly journals Estrogenic Activity of Ethanolic Extract of Papaya Peels (Carica Papaya L.) on Uterine Weight and Mammae Gland Proliferation on Ovariectomy Rats

2018 ◽  
Vol 9 (2) ◽  
pp. 86 ◽  
Author(s):  
Dhania Novitasari ◽  
Devyanto Hadi Triutomo ◽  
Fitriana Hayyu Arifah ◽  
Anselma Ivanawati ◽  
Zahrotul Ulum ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Uterine Weight ◽  
Sprague Dawley ◽  
In Vivo Test ◽  
Female Reproductive Health ◽  
Agnor Staining

Papaya bark is one of Indonesia's natural wealth that contains flavonoid compounds such as myricetin and kaempferol that included in the phytoestrogen compounds. The aim of this study is to examine the estrogenic effects of ethanolic extract of papaya peels (EEPP), on the development of mammae gland and the increasing of uterine weight. The in vivo test was performed with ovariectomy in Sprague Dawley female rats that caused the rats to be in an estrogen deficiency state. After 30 days of treatment, animals are sacrificed to take the uterus and mammae glands. Measurement of uterine weight and mammae gland are observed by hematoxylin-eosin staining method to know the lobulus development and AgNOR staining to determine the proliferation level of mammae gland epithelial cells. The test results showed that EEPP concentration of 500 and 1000 mg/kgBW were able to increase uterine weight and proliferation of mammae gland. From the results of this study, papaya bark has the potential to be one of the phytoestrogens compound to maintain female reproductive health and woman beauty.Keyword: ethanolic extract of papaya peels (EEPP), phytoestrogen, ovariectomized rats, uterine weight, mammae proliferation

scholarly journals Effects of estrogen-like plant compounds on the vaginal epithelium pituitary, adrenal glands, and uterus of rats

2018 ◽  
Vol 243 (15-16) ◽  
pp. 1173-1184
Author(s):  
Gismar MC Rodrigues ◽  
Bruno DB Borges ◽  
Leticia Gabriela Q Moreira ◽  
Érica Aparecida G Rossete ◽  
Suzelei de Castro Franca
Keyword(s):  
Estrogen Receptor ◽  
Mechanism Of Action ◽  
Adrenal Glands ◽  
Estrogenic Activity ◽  
Reproductive Organs ◽  
Colocasia Esculenta ◽  
Vaginal Epithelium ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Prolonged Treatment

Plant species with recognized estrogenic activity and widely used by Brazil’s female population to prevent the unpleasant symptoms of menopause were investigated in this work to demonstrate if constituents of taro-inhame ( Colocasia esculenta), cumaru ( Dipteryx odorata), and camapu ( Physalis angulata) have the ability to mimic or interfere with the action of estrogens. Moreover, their potential use as natural sources of estrogen-like substances for hormone replacement therapy was evaluated. (a) In vivo pharmacological assays were conducted to determine the estrogenic effects of D. odorata isoflavone-rich extract, P. angulata physalin-rich extract, and C. esculenta flavonoid glycoside-rich fraction (FG) on endocrine glands and reproductive organs of female rats at three different stages of the life cycle. The protocols consisted of uterotrophic assays and cytological evaluation of vaginal smears to detect mucosa cell alterations correlated with changes in hormone levels in each phase of the estrous cycle of female rats. The results indicated that C. esculenta FG exhibited estrogenic activity in prepubescent, pubescent, and adult ovariectomized female rats, while D. odorata isoflavones only promoted a weight increase in the pituitary gland of prepubescent rats after prolonged treatment and P. angulata physalins induced a weight increment in the adrenal glands of ovariectomized rats. Additionally, C. esculenta exerted a significant effect on the opening of the vaginal canal in prepubescent rats and on vaginal epithelium. Prolonged treatment of ovariectomized rats with FG altered the proportion of different types of vaginal epithelial cells in these animals, suggesting an interference of FG with estrogen levels. Colocasia esculenta FG induced hypertrophy of the uterus and pituitary in ovariectomized rats similar to estradiol. To elucidate the mechanism of action of FG, its effects were compared to those of estradiol and of the selective estrogen receptor modulator raloxifene. The results suggest that the efficacy of C. esculenta FG is mediated by binding to selective estrogen receptors present in each organ and that raloxifene inhibits the mechanism of action of FG in the same way as it inhibits the effects of estradiol. The overall findings indicate that C. esculenta FG mimics the action of estrogens, with reduced harmful effects on specific tissues. Impact statement The results of this work provide preliminary evidence that ovariectomized rats display the vaginal smear cells typical of the estrous phase of mature rats after long-term ingestion of Colocasia esculenta flavonoid glycosides. Moreover, the treatment caused beneficial effects on endocrine and reproductive organs compared to estradiol. The animals did not exhibit significant body weight alterations among the groups, demonstrating the maintenance of standard metabolism and energy balance. The overall findings obtained with the rat model of menopause highlight the importance of dietary consumption of C. esculenta constituents as potential selective estrogen receptor modulators and suggest that they may prevent some of the metabolic disorders related to estrogen deficiency.

scholarly journals Estrogenic Activity of Mahoni Seed Ethanolic Extract [Swietenia mahogany (L.) Jacq] on Uterus Weight, Bone Density and Mamae Gland Proliferation on Ovariectomized Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 75
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Rosa Gloria Sitanggang ◽  
Robbani Syahfitri Angkat
Keyword(s):  
Bone Density ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Group 2 ◽  
Post Hoc

Menopause is a hypoestrogenic condition due to decreased function of the ovary. During menopause there is no reserved ovum in the ovary, as a result the synthesis of estrogen by the follicles does not take place. Deficiency of estrogen can lead to discomfort and decrease in the women quality of life. Therefore, supplements from natural resources to reduce menopausal symptoms will be needed. The objectives of the study were to determine the effect of mahogany seeds ethanolic extract (MSEE) on the development of uterus, bone density, and mammae gland proliferation on ovariectomized rats. Extract was made by maceration using 96% ethanol as the solvent, then the study of estrogenic effect was carried out on 30 female rats which were divided into 6 groups. Group 1 (normal control), group 2 (positive control) given estradiol dose of 0.18 mg/kg body weight (BW), group 3 (negative control) given Na-CMC 1% and group 4, 5, 6 given MSEE orally for 14 consecutive days with doses of 50, 100, 200 mg/kg BW. Data were analysed using ANOVA then continued with Tukey HSD Post Hoc test to see the differences between the treatments. The results of the study showed that MSEE was able to increase the weight of the uterus, the length of estrus phase in the estrus cycle, bone density and the mammae gland proliferation of rats. The results concluded that MSEE has phytoestrogenic effect on ovariectomized rats.Keywords: phytoestrogen, ovariectomy, uterus weight, bone density, mammae proliferation

Estrogenic Activity Produced by Aqueous Extracts of Silkworm (Bombyx mori) Pupae in Ovariectomized Rats

2010 ◽  
Vol 38 (01) ◽  
pp. 89-97 ◽  
Author(s):  
Hyo-Jung Yang ◽  
Jeong-Woong Lee ◽  
So-Hyun Lee ◽  
Jae-Sung Ryu ◽  
Dong-Hoon Kwak ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Estrogenic Activity ◽  
The Body ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Aqueous Extracts ◽  
Ovx Rats ◽  
Silkworm Pupae ◽  
Body Weights ◽  
Silkworm Bombyx Mori

This study examined the estrogenic activity produced by aqueous extracts of silkworm (Bombyx mori) pupae in ovariectomized (OVX) rats. The components of silkworm pupae were extracted in distilled water at room temperature for 6 hours. The ovaries of six-week old female rats were then bilaterally removed. One week after OVX, the animals were treated with 200, 400 or 600 mg/kg/day of silkworm pupae extracts. The body weights of the OVX rats increased remarkably compared to the control rats, however their relative uterus weights to body weights decreased significantly. Treatment with the aqueous extracts of silkworm pupae dramatically improved the decreased uterus weights of OVX rats, with the highest increase observed in treatment with 200 mg/kg/day of the aqueous extracts. Additionally, treatment with aqueous extracts (200 mg/kg/day) of silkworm pupae significantly elevated the serum 17β-estradiol contents of OVX rats when compared to the control animals. To examine the toxic effects of silkworm pupae on the hepatic functions of OVX rats, the levels of serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were measured. The serum GOT and GPT levels did not change in response to the administration of aqueous extracts (200, 400 and 600 mg/kg/day) for 4-weeks. Taken together, these results suggest that the aqueous extracts of silkworm pupae may have estrogenic activity, which suggests that silkworm pupae may be useful in the prevention and/or treatment of menopausal disorders caused by deficiencies in female sexual hormones, including estrogen.

scholarly journals Depressive Behavior of Rats Consuming Cocoa Powder and Cocoa Extract

2018 ◽  
Vol 34 (2) ◽  
pp. 90-94
Author(s):  
Ariza Budi Tunjung Sari ◽  
Misnawi Misnawi ◽  
Pratiwi Pudjiastuti ◽  
Afaf Baktir
Keyword(s):  
Treatment Time ◽  
Estrogenic Activity ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Vehicle Group ◽  
Estradiol Valerate ◽  
Depressive Behavior ◽  
Cocoa Powder ◽  
Immobility Time ◽  
Significant Difference

Chocolate is associated with calm and happy feelings. However little is found to demonstrate if this effect is induced by active ingredient in cocoa ratherthan the sweetness of chocolate. In this study we tested the effect of cocoa on the depressive behavior of female rats suffering from estrogen deficiency. Twentyfemale rats aged seven weeks old were ovariectomized to remove the estrogen source. The rats were distributed into four groups and undergoing three dayoral administration of 1g/kg body weight (bw) cocoa powder, 1g/kg bw cocoa extract, 1 mg/kg bw estradiol valerate and 1 mL/kg bw vehicle (olive oil). Anothergroup of rats having intact ovaries received 1 mL/kg bw drinking water. The rats were undergoing forced swimming test (FST) one day before treatment and after treatment and the immobility time was recorded. No significant difference was found on the after-treatment immobility time across groups. However, all the rats in the vehicle group developed longer immobility time after-treatment compared with the result from before-treatment test, suggesting that depression had occurred in ovariectomized rats receiving no active substances. In the other hand, very few rats had greater after-treatment immobility time in the cocoa powder and estradiol valerate groups. In the cocoa extract group, all the rats had shorter immobility time after treatment when it is compared with before treatment time. We conclude that compounds in cocoa have certain extent of estrogenic activity that affects emotional state.

scholarly journals Ameliorated Impact of Fenugreek Seed Extract on Some Blood Cellular and Biochemical Parameters in Female Albino Rats Exposed to Lead Acetate

2020 ◽  
pp. 105-115
Author(s):  
Rozhya Abduljalil Naser ◽  
Rabar Yousif Mameik ◽  
Nazar M. Shareef Mahmood ◽  
Kurdo Bapir Chato
Keyword(s):  
Lead Acetate ◽  
Biochemical Parameters ◽  
Basal Diet ◽  
Seed Extract ◽  
Female Rats ◽  
Albino Rats ◽  
Fenugreek Seeds ◽  
Fenugreek Seed ◽  
Ad Libitum ◽  
Trigonella Foenum Graecum L

Lead acetate (LA) is considered as the common hazardous compund present in our habitat. It has a several diverse-organ toxicant effect in various healthy issues included diseases of the hepatic, renal, and other body systems. Fenugreek seeds (Trigonella foenum graecum L.) were used in curing medicine and as food supplement since ancient times. After that, their antioxidant and hepatoprotective properties have been studied. Therefore the presented was based on their biological properties of this plant and conducted to examine the protective impact of fenugreek seed extract upon LA toxicity on some blood cellular and biochemical parameters of female albino rats (Rattus norvegicus). Twenty eight female rats were randomly and equally divided into four groups each one contain seven rats as following; first group received basal diet and tap water ad libitum. Second group received basal diet and LA 80 mg/L with water ad libitum. Third group received basal diet contained 2.5% fenugreek seed extract and LA 80 mg/L with water ad libitum. Fourth group received basal diet contained 2.5% fenugreek seed extract with water ad libitum. Rat’s administration was continued daily for two weeks respectively. The administration of rats with LA produced significant reduction in liver weight, food intake, while it increased triglyceride (TG), alkaline phosphatase (ALP), aspartate aminotransferase (AST), uric acid (UA) and creatinie (CR). While supplemented diet with 2.5% Fenugreek seed extract improved body weight, organ weight, lipid peroxidation, TG, ALP, and AST. Our results plead for the profiteering of fenugreek seeds as a dietary supplement, because it showed protective effect of their content in polyphenolic flavonoids, antioxidant and membrane-protective effects, against exposure to the LA compound to protect injurious risk.

Effect Of Bangun-Bangun Leaves Ethanol Extract On Lipid Profile Ovariectomized Female Rats

2021 ◽  
Vol 4 (1) ◽  
pp. 58-65
Author(s):  
Suharyanisa ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Pandapotan Nasution
Keyword(s):  
Lipid Profile ◽  
Estrogenic Activity ◽  
Ethanol Extract ◽  
The Body ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Post Hoc

Menopause was a condition of the body when ovaries stopped producing ovum; ovum is the main producer of estrogen hormone. Menopause can increasing lipid profile in blood; it causes any problems in the cardiovascular system. People of North Sumatra have long used Bangun-Bangun leaves as a breastfeeding agent; it contains flavonoid bioactive compounds, which are be expected to replace lost estrogen hormone. This study determined the estrogenic activity of Bangun-Bangun leaves ethanol extract (BBLEE) on lipid levels of ovariectomized female rats as a menopausal model. BBLEE has obtained by maceration techniques with 96% ethanol and extract used on 24 ovariectomized rats which were divided into 6 groups. Group 1 (normal), group 2 (positive) was treated by estradiol at dose of 0.18 mg/kg BW, group 3 (negative) was treated by 0.5% Na-CMC and group 4, 5, 6 were treated by BBLEE at dose of 30, 60, 90 mg/kg BW given orally for 14 days. The data were analyzed by ANOVA and Post Hoc Tukey HSD test. The result showed that effective dose of BBLEE was 90 mg/kg BW which improved lipid profile (TC = 55.75±0.47; TG = 63.00±0.40; HDL = 37.00±0.81; LDL = 30.50±0.86) in blood of ovariectomized female rats as a menopausal model. BBLEE at the dose of 90 mg/kg BW can ameliorate the lipid profile in the blood of ovariectomized female rats as a menopausal model.

scholarly journals Investigation of the estrogenic activity of Pueraria candollei variety mirifica extract on rats

2019 ◽  
Vol 8 (10) ◽  
pp. 2172
Author(s):  
Dao Thi Vui ◽  
Nguyen Thu Hang ◽  
Nguyen Quoc Huy ◽  
Bui Thanh Tung
Keyword(s):  
Estrogenic Activity ◽  
Reproductive Organs ◽  
Mature Female ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Serum Estradiol ◽  
Immature Female ◽  
Pueraria Candollei ◽  
Immature Rats ◽  
Estradiol Concentration

Background: Pueraria candollei variety mirifica (PM) has been widely used as ingredient in many rejuvenating products. In this study, we aimed to assess the estrogenic activity of PM extract grown in Vietnam.Methods: Estrogenic activity of PM extract was estimated on immature female rats by using uterotrophic method to measure the weight of the reproductive organs. Estrogenic activity of PM extract also was investigated in mature female ovariectomized rats by evaluating the vaginal cells growth, reproductive organs weight, serum estradiol concentration.Results: Our results showed that PM extract at doses of 100 mg/kg, 200 mg/kg had increased the reproductive organs weight in immature rats and female ovariectomized rats. In addition, PM extract had increased the serum estradiol concentration and the vaginal cells growth by increasing the percentage of keratinocytes in female ovariectomized rats.Conclusions: Our results showed that PM extract has strong estrogenic activity in rats.

scholarly journals Less Carcinogenic Chlorinated Estrogens Applicable to Hormone Replacement Therapy

2021 ◽  
Vol 22 (13) ◽  
pp. 7222
Author(s):  
Yoshinori Okamoto ◽  
Hideto Jinno ◽  
Shinji Itoh ◽  
Shinya Shibutani
Keyword(s):  
Hormone Replacement Therapy ◽  
Replacement Therapy ◽  
Hormone Replacement ◽  
Metabolic Activation ◽  
Ovariectomized Rats ◽  
Estrogenic Potential ◽  
Carcinogenic Potential ◽  
Induced Tumors ◽  
Carcinogenic Effects ◽  
17Β Estradiol

Human estrogens prescribed for hormone replacement therapy (HRT) are known to be potent carcinogens. To find safer estrogens, several chlorinated estrogens were synthesized and their carcinogenic potential were determined. A pellet containing either 2-chloro-17β-estradiol (2-ClE2) or 4-chloro-17β-estradiol (4-ClE2) was implanted subcutaneously for 52 weeks into August Copenhagen Irish (ACI) rats, a preferred animal model for human breast cancer. 17β-Estradiol (E2) frequently induced mammary tumors while both 2-ClE2 and 4-ClE2 did not. Their 17α-ethinyl forms, thought to be orally active estrogens, were also synthesized. Neither 2-chloro-17α-ethinylestradiol (2-ClEE2) nor 4-chloro-17α-ethinylestradiol (4-ClEE2) induced tumors. The less carcinogenic effects were supported by histological examination of mammary glands of ACI rats treated with the chlorinated estrogens. A chlorine atom positioned at the 2- or 4-position of E2 may prevent the metabolic activation, resulting in reducing the carcinogenicity. 2-ClE2 and 4-ClE2 administered subcutaneously and 2-ClEE2 and 4-ClEE2 given orally to ovariectomized rats all showed uterotrophic potency, albeit slightly weaker than that of E2. Our results indicate that less carcinogenic chlorinated estrogens retaining estrogenic potential could be safer alternatives to the carcinogenic estrogens now in use for HRT.

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