Effect of Seed Priming Techniques on Germination Parameters of Safflower (Carthamus tinctorius L.)

Summary A useful approach for improving seed germination and seedling growth is a seed priming technique. Application of the priming technique enhances water absorption, causing activation of metabolic activities in the seed. The objective of this study was to evaluate the effect of seed priming on germination parameters of safflower and to compare different priming techniques: priming by soaking and priming on filter paper. The priming treatments included hydropriming (distilled water) and osmopriming with 0.1% and 0.5% solutions of KNO3 for 8 and 16 hours. The experiment revealed significant difference between the priming treatments and the control. The highest germination (89.50%) was recorded within the priming treatments by soaking in the solution of 0.1% KNO3 and priming on filter paper moistened with 0.5% KNO3 for 8 hours. Considering germination index, mean germination time and time to 50% germination, the best results were obtained within hydropriming on filter paper for 16 hours. This study has shown that the priming techniques significantly improved germination parameters of safflower. Although priming on filter paper showed better results, the soaking technique – due to its simplicity, low cost and easiness of application – can be successfully used to improve germination parameters of safflower and increase the number of plants per unit of area and thus increase the seed yield per acreage.

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GIBBERELLIC ACID PRIMING ENHANCES MAIZE SEED GERMINATION UNDER LOW WATER POTENTIAL

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v22n1.2021.p17-26 ◽

2021 ◽

Vol 22 (1) ◽

pp. 17

Author(s):

Amit Gnawali ◽

Roshan Subedi

Keyword(s):

Gibberellic Acid ◽

Water Potential ◽

Low Cost ◽

Shoot Length ◽

Maize Seedlings ◽

Germination Time ◽

Stressful Environment ◽

Mean Germination Time ◽

Low Water Potential ◽

Germination Parameters

Germination is a portentous yield determining factor that is a challenge in the low water potential environment due to disrupted imbibition. Hormonal seed priming can markedly increase the germination on maize even in such a stressful environment. Therefore, research was aimed to analyze the application of gibberellic acid (GA3) priming to maize seeds to minimize the deleterious effects of reduced water potential. GA3 priming was done at 0, 50, 100, 150, and 200 ppm for 12 hours and subjected to drought levels of 0, 0.15, 0.50, 1.05, and 1.75 MPa by using NaCl solution. Different germination parameters, i.e., germination percentage, mean germination time, germination index, relative water content, seedling vigor index, root length, and shoot length, were determined on the final day of the experiment. The results showed that all germination parameters were adversely influenced by low water potential. Every level of GA3 priming has hastened all parameters. GA3 priming at 100 ppm decreased the mean germination time by 35 hours under 1.75 MPa compared to non-primed seeds. GA3 priming increased the shoot length of maize seedlings with a shoot length of 2.9 cm in non-primed seedlings compared to 6.4 cm in 200 ppm GA3 priming under 1.05 MPa. GA3 priming is the best method for the early establishment of maize seedlings in low water potential conditions. As a result, it may be utilized as a low-cost and straightforward approach for establishing maize crops under saline and drought conditions.

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Impact of Organic Seed Priming Amendments and Botanicals on Seed Quality Parameters of French Bean (Phaseolous vulgaris L.)

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2021.1011.030 ◽

2021 ◽

Vol 10 (11) ◽

pp. 259-265

Author(s):

Prashant Kumar Rai Kotamarthi Bhavana ◽

G. M. Lal

Keyword(s):

Leaf Extract ◽

Seed Quality ◽

Low Cost ◽

Organic Amendments ◽

Seed Priming ◽

Quality Parameters ◽

French Bean ◽

Distilled Water ◽

Seed Vigour ◽

Vigour Index

The current experiment was conducted to evaluate the Performance of Organic amendments and botanical treatments on seed quality parameters of French bean under stimulated environmental conditions. An experiment was conducted in 2021 at postgraduate seed testing laboratory, Department of Genetics and Plant Breeding Naini Agriculture Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. The French bean Cultivar Arka Bold seeds were used for priming. Organic treatments are designated as Panchagavya 2%, 4%,6%,8%, Agniasthra 2%,4%,6%,8% and Botanicals treatments are Tulasi leaf extract 5%, Aloevera leaf extract 5%, Vinca rosea leaf extract 5% and also did hydroprming treatment using distilled water respectively. Seeds were soaked in organic, botanicals and in distilled water for duration of 6 hours followed by shade drying. The results were obtained and it was found that all the treatments showed significant effect on quality parameters. The highest was found in panchgavya of all doses followed by tulasi leaf extract found to be best in Germination percentage, Speed of Germination, Root length, Shoot length, Seedling length, Seed vigour index-I, Seed vigour Index-II compared to control or untreated seeds which indicates the effect of organics and botanicals on physiological processes of the seeds. This study revealed that priming with organic amendments and botanicals can be effective in enhancing the seed quality parameters of french bean and they are low cost, effective and nature friendly and toxic free.

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Seed Germination and Initial Growth of Pepper Seedlings is Influenced by Environment Temperature and Priming Method

Journal of Agricultural Studies ◽

10.5296/jas.v8i4.17831 ◽

2020 ◽

Vol 8 (4) ◽

pp. 540

Author(s):

Glenda Sallaku ◽

Gjok Vuksani ◽

Astrit Balliu

Keyword(s):

Polyethylene Glycol ◽

Germination Percentage ◽

Early Growth ◽

Distilled Water ◽

Initial Growth ◽

Environment Temperature ◽

Germination Speed ◽

Mean Germination Time ◽

Peg 8000 ◽

Germination Parameters

The effects of different environment temperatures, priming treatments and priming durations on germination parameters and early growth of pepper seedlings were investigated. Seeds were hydro primed in distilled water, or osmo primed in two different water potentials (-0.15 and -0.5 MPa) of polyethylene glycol (PEG) 8000. Both, hydro primed and osmo primed seeds were subject of two different imbibitions periods, 48 and 72 hrs. In addition to primed seeds, an equal sample of non primed seeds was included in the experiment as a control variant. Following priming, the seeds were subjected to germination tests at two different environment temperatures, respectively 18 0C and 24 0C. In general, there was no significant effect of hydro priming on germination parameters or early growth of pepper seedlings. On the contrary, significantly higher germination percentage and germination speed and significantly shorter mean germination time than the non primed seeds were obtained due to seed osmo priming by PEG. Similarly, an enhanced seedling growth, especially under suboptimum temperature conditions was evidenced in PEG primed seedlings. Apart from germination speed, the increase of PEG concentration from -0.15 to -0.5 MPa had no effect to germination parameters of pepper seeds. The extent of the imbibitions period over 48 hrs did also have no benefits on germination parameters.

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Evaluation of several methods for breaking dormancy of bitter vetch seeds (Vicia ervilia L.)

Plant Breeding and Seed Science ◽

10.1515/plass-2015-0022 ◽

2015 ◽

Vol 71 (1) ◽

pp. 57-65 ◽

Cited By ~ 1

Author(s):

Hossein Reza Rouhi ◽

Ali Sepehri ◽

Leila Sefidkhani ◽

Fatemeh Karimi

Keyword(s):

Germination Percentage ◽

Hot Water ◽

The Other ◽

Cold Stratification ◽

Distilled Water ◽

Breaking Dormancy ◽

Bitter Vetch ◽

Physiological Dormancy ◽

Mean Germination Time ◽

Germination Parameters

Abstract This study analysed the effects of different treatments on breaking dormancy and germination of bitter vetch (Vicia ervilia L.) freshly harvested seeds for seeding immediately. Partial scarified seeds (30 seconds with sandpaper) were subjected to different treatments including: GA3 (250, 500 and 750 ppm), KNO3 (0.1, 0.2 and 0.3% w/v), cold stratification (2, 4 and 6 days), sulfuric acid (25, 50 and 75 seconds), hot water (90°C; for 2.5 and 5 minutes), hydropriming via seed soaking in distilled water (4 and 8 hours) and mechanical scarification. Among the mentioned treatments, cold stratification for 6 days had a best effect on germination related parameters final germination percentage, mean germination time and vigour indexthan the other periods. In contrast to scarification with acid and hot water, mechanical scarification improved germination parameters but this effect was lower than the cold stratification. The results suggest that bitter vetch seed has both physical and physiological dormancy.

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Aerosolised fluorescein can quantify FFP mask faceseal leakage: a cost-effective adaptation to the existing point of care fit-test.

10.21203/rs.3.rs-104710/v1 ◽

2020 ◽

Author(s):

Sameer Zaman ◽

Henry Seligman ◽

Freya Hepworth Lloyd ◽

Keval Tushar Patel ◽

Digby Chappell ◽

...

Keyword(s):

Filter Paper ◽

Point Of Care ◽

Low Cost ◽

Cost Effective ◽

Test Protocol ◽

Taste Test ◽

Significant Difference ◽

Fit Testing ◽

Total Fluorescence ◽

Low Levels

Abstract Background Filtering facepiece (FFP) respirators must provide an adequate faceseal to protect healthcare workers from harmful particles. A qualitative fit-test using bitter-tasting aerosols the commonest way to determine if an FFP mask is safe enough for clinical use. This taste-test is subjective and can be biased by placebo. We propose a cheap and quantitative modification of the taste-test, by measuring the amount of fluorescein staining filter paper behind the FFP mask after a fit-test protocol, using digital image analysis. Methods Medical grade fluorescein was added to bitter-tasting denatonium benzoate solution and Aerosolised during a mask fit-testing protocol. Scientific filter paper was placed on the inner surface of the mask. Participants were asked if they could taste the solution to determine their qualitative ‘pass’ or ‘fail’ result. Filter paper photographs were analysed after the test to quantify total fluorescence (TF). TF levels in the taste-test ‘pass’ and ‘fail’ groups were compared.Results Fifty-six healthcare professionals completed the fluorescein mask fit-test protocol. 32 (57%) ‘passed’ the qualitative (taste) test and the remainder ‘failed’. There was a significant difference in TF between the groups based on their qualitative results (p <0.001). A cut-off of TF = 5.0 x 106 fluorescence units was determined by analysing the precision (78%) and recall (84%) of the fluorescein test. Applying this cut-off resulted in 5 out of 56 participants (9%) being reclassified from ‘pass’ to ‘fail’ by the fluorescein test. 7 out of 56 (12%) participants were reclassified from ‘fail’ to ‘pass’.Conclusions Fluorescein is detectable and sensitive to identify faceseal leaks in FFP masks. The fluorescein fit-test is discriminating in its ability to divide people into ‘pass’ and ‘fail’ groups similarly to the taste-test. The adaptations are low-cost and could be incorporated in the point-of-care setting. After further validation the fluorescein test could increase safety for staff by reducing the number of false ‘pass’ by the taste-test. It could also reassure people who have ‘failed’ the taste-test that they have low levels of fluorescein leak, enabling them to return to clinical practice safely.

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Nutripriming with zinc sulphate and borax for early growth and seedling vigour in grain cowpea [Vigna unguiculata (L.) Walp]

Legume Research - An International Journal ◽

10.18805/lr-4099 ◽

2019 ◽

Author(s):

Anju B Raj ◽

Sheeja K Raj ◽

K. Prathapan ◽

N.V. Radhakrishnan

Keyword(s):

Germination Rate ◽

Seed Priming ◽

Early Growth ◽

Research Station ◽

Seedling Vigour ◽

Early Seedling Growth ◽

Vigour Index ◽

Early Seedling ◽

Mean Germination Time ◽

Germination Parameters

The experiment was conducted at Coconut Research Station, Balaramapuram with an objective to study the effect of nutripriming with ZnSO4 and borax on the early growth and seedling vigour and Zn and B content in grain cowpea. Nutripriming treatments had significant effect on germination parameters. Nutripriming with ZnSO4 either at 0.025 or 0.05 per cent recorded higher values for the germination parameters. These treatments recorded 19.68 and 18.51 per centage higher germination than control. Germination index was 1.4 times higher in seeds primed with ZnSO4 @ 0.025 and 0.05 per cent and also recorded higher values for mean daily germination and speed of germination. The highest co-efficient rate of germination and germination rate index was recorded by seed priming with ZnSO4 at 0.05 per cent and it was closely followed by ZnSO4 @ 0.025 per cent for 4 h. Nutripriming with ZnSO4 either @ 0.025 or 0.05 per cent recorded the lowest mean germination time and time to reach 50 per cent germination and also recorded higher Zn and B content. Vigour index also followed the same trend. Hence it can be concluded that nutripriming with ZnSO4 either at 0.025 or 0.05 per cent can be recommended for early seedling growth, seedling vigour and higher Zn and B content in grain cowpea.

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Using seed respiration as a tool for calculating optimal soaking times for ‘on-farm’ seed priming of barley (Hordeum vulgare)

Seed Science Research ◽

10.1017/s0960258521000039 ◽

2021 ◽

pp. 1-9

Author(s):

Javier Carrillo-Reche ◽

Adrian C. Newton ◽

Richard S. Quilliam

Keyword(s):

Morphological Changes ◽

Low Cost ◽

Germination Rate ◽

Seed Priming ◽

Embryonic Axes ◽

Seedling Vigour ◽

Soaking Time ◽

On Farm ◽

Seed Respiration

Abstract A low-cost technique named ‘on-farm’ seed priming is increasingly being recognized as an effective approach to maximize crop establishment. It consists of anaerobically soaking seeds in water before sowing resulting in rapid and uniform germination, and enhanced seedling vigour. The extent of these benefits depends on the soaking time. The current determination of optimal soaking time by germination assays and mini-plot trials is resource-intensive, as it is species/genotype-specific. This study aimed to determine the potential of the seed respiration rate (an indicator of metabolic activity) and seed morphological changes during barley priming as predictors of the priming benefits and, thus, facilitate the determination of optimal soaking times. A series of germination tests revealed that the germination rate is mostly attributable to the rapid hydration of embryo tissues, as the highest gains in the germination rate occurred before the resumption of respiration. Germination uniformity, however, was not significantly improved until seeds were primed for at least 8 h, that is, after a first respiration burst was initiated. The maximum seedling vigour was attained when the priming was stopped just before the beginning of the differentiation of embryonic axes (20 h) after which vigour began to decrease (‘over-priming’). The onset of embryonic axis elongation was preceded by a second respiration burst, which can be used as a marker for priming optimization. Thus, monitoring of seed respiration provides a rapid and inexpensive alternative to the current practice. The method could be carried out by agricultural institutions to provide recommended optimal soaking times for the common barley varieties within a specific region.

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Quantification and Classification of Diclofenac Sodium Content in Dispersed Commercially Available Tablets by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Pharmaceuticals ◽

10.3390/ph14050440 ◽

2021 ◽

Vol 14 (5) ◽

pp. 440

Author(s):

Eirini Siozou ◽

Vasilios Sakkas ◽

Nikolaos Kourkoumelis

Keyword(s):

Infrared Spectroscopy ◽

Low Cost ◽

Diclofenac Sodium ◽

Reference Method ◽

Sodium Content ◽

Attenuated Total Reflection ◽

Accuracy And Precision ◽

Significant Difference ◽

Ft Ir

A new methodology, based on Fourier transform infrared spectroscopy equipped with an attenuated total reflectance accessory (ATR FT-IR), was developed for the determination of diclofenac sodium (DS) in dispersed commercially available tablets using chemometric tools such as partial least squares (PLS) coupled with discriminant analysis (PLS-DA). The results of PLS-DA depicted a perfect classification of the tablets into three different groups based on their DS concentrations, while the developed model with PLS had a sufficiently low root mean square error (RMSE) for the prediction of the samples’ concentration (~5%) and therefore can be practically used for any tablet with an unknown concentration of DS. Comparison with ultraviolet/visible (UV/Vis) spectrophotometry as the reference method revealed no significant difference between the two methods. The proposed methodology exhibited satisfactory results in terms of both accuracy and precision while being rapid, simple and of low cost.

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Robust immobilization of anionic silver nanoparticles on cellulose filter paper toward a low-cost point-of-use water disinfection system with improved anti-biofouling properties

RSC Advances ◽

10.1039/d0ra09152a ◽

2021 ◽

Vol 11 (9) ◽

pp. 4873-4882

Author(s):

Gongyan Liu ◽

Ruiquan Yu ◽

Jing Jiang ◽

Zhuang Ding ◽

Jing Ma ◽

...

Keyword(s):

Silver Nanoparticles ◽

Filter Paper ◽

Low Cost ◽

Water Disinfection ◽

Point Of Use ◽

Cellulose Filter

Point-of-use water disinfection by GA@AgNPs-LA-FP.

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Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

Seed Science Research ◽

10.1017/s0960258520000409 ◽

2020 ◽

pp. 1-9

Author(s):

Keum-Ah Lee ◽

Youngnam Kim ◽

Hossein Alizadeh ◽

David W.M. Leung

Keyword(s):

Oxidative Stress ◽

Amino Acids ◽

Salinity Stress ◽

Lolium Multiflorum ◽

Casein Hydrolysate ◽

Seed Priming ◽

Germination Percentage ◽

Italian Ryegrass ◽

Significant Difference ◽

Protein Amino Acids

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

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