scholarly journals Extraction of acyclovir from pharmaceutical creams for HPLC assay. Optimization and validation of pretreatment protocols

2008 ◽  
Vol 6 (2) ◽  
pp. 140-144 ◽  
Author(s):  
Paraskevas Tzanavaras ◽  
Constantinos Zacharis

AbstractThree simple protocols for the extraction of acyclovir from its pharmaceutical creams based on ultrasonication, ultrasonication with heating and magnetic stirring were evaluated and compared. Extraction kinetics were studied at different time intervals (5, 10, 15, 30 and 60 min) and the extraction efficiency was determined by HPLC. The effect of concentration of aqueous NaOH as the extraction medium and the stirring speed were also studied and optimized. Best results were obtained with 50 mL of 0.01 mol L−1 aqueous NaOH with magnetic stirring speed of 500 r.p.m. HPLC analysis involved rapid separation of acyclovir from the cream matrix using a 100 × 4.6 mm i.d. monolithic column and UV detection at 254 nm. Magnetic stirring produced the best results in terms of extraction efficiency with an average extraction yield of 100.8%, n = 16 at an optimum extraction time of 15 min. The selected protocol was validated for within and day-to-day precision and ruggedness.

2013 ◽  
Vol 634-638 ◽  
pp. 1049-1053
Author(s):  
Hui Lin Cao ◽  
Shuang Ran Liang ◽  
Yang Tian ◽  
Zhi Ling Chen ◽  
Jian Ping Chao

Microwave-assisted steam extraction system combines the advantages of both conventional and modern technologies. MAE is probably going to be the leading technology in essential oil producing industry. In our research, one-factor and uniform experimental designs are used to determine the optimum extraction conditions, and the results are as follows: the extraction yield is measured as 2.30% with 3h of reflux time, when degree of pulverization is 90, immersing time is 3h, yield of solid to liquid equals 12: 1 ml/g. The chemicals in the production were examined by GC-MS. More than 40 kinds of chemicals were detected, which is consistent with that of the traditional steam extraction method. However, the extraction efficiency is dramatically increased when the steam extraction is assisted with microwave heating, and it is up to 3.8 times.


2019 ◽  
Vol 102 (2) ◽  
pp. 680-682
Author(s):  
Junjian Ran ◽  
Xinhong Liang ◽  
Hanmei Du ◽  
Junliang Sun

Abstract Background: A lot of sweet potato residues (SPR) were discarded and wasted. Objective: To make full use of the SPR. Methods: Ultrasonic microwave synergistic (UMS) extraction method was used to extractdehydroepiandrosterone (DHEA) in SPR. The extraction conditions were optimized by response surface methodology based on single factors. Results: The optimum extraction conditions were 1:25 (solid–liquid ratio), 300 W (microwave power), 30 min (extraction time), and 30°C (extraction temperature). The extraction yield of DHEA from SPR reached 117.25 μg/100 g. Conclusions: The advantage of UMS extractiontechnology is to make full use of the synergistic effect of ultrasound and microwave to improve extraction efficiency. Highlights: The technology provides an effective way to improve the DHEA extraction yield from the SPR in industrial production.


Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2158 ◽  
Author(s):  
Florina Dranca ◽  
Mircea Oroian

Pectin was extracted from apple (Malus domestica ‘Fălticeni’) pomace with Celluclast 1.5L, at doses of 20, 40, and 60 µL/g of material. The temperature and time of extraction were varied on three levels—temperature—40, 50, and 60 °C; time—12, 18, and 24 h. For each experiment, the extraction yield (R2 = 0.8905), the galacturonic acid content (R2 = 0.9866), and the degree of esterification (R2 = 0.9520) of pectin was determined. Response surface methodology (RSM) was implemented via a Box–Behnken design, to optimize pectin extraction. In the optimum extraction conditions (temperature of 48.3 °C, extraction time of 18 h 14 min, and enzyme dose of 42.5 µL/g of pomace), the design predicted a 6.76% yield with a galacturonic acid content of 97.46 g/100 g of pectin and a degree of esterification of 96.02%. FT-IR analysis of the pectin sample obtained in these conditions showed a chemical structure similar to that of commercial apple and citrus pectin.


2018 ◽  
Vol 10 (6) ◽  
pp. 211
Author(s):  
Eka Indra Setyawan ◽  
Erna Prawita Setyowati ◽  
Abdul Rohman ◽  
Akhmad Kharis Nugroho

Objective: This study was intended to optimize the extraction condition using central composite design.Methods: Central composite cesign with three independent variables, namely water temperature, brewing time, and brewing number were used to obtain the optimum extraction condition. Two dependent variables, namely yield of extraction and epigallocatechingallate level were used as a response parameter. Epigallocatechin gallate level was determined by using high-performance liquid chromatography method.Results: Extraction yield was varied from 0.30 g to 0.72 g. All variables, namely water temperature, brewing time, and brewing number were able to increase the extraction yield. Epigallocatechingallate level was varied from 190.23 mg/g to 301.74 mg/g. Water temperature, brewing time, and both interaction were able to increase the epigallocatechin gallate level in green tea extract.Conclusion: Optimum extraction condition was shown using hot water at a temperature of 95 °C for 20 min and two-times infusions. The condition obtained extraction yield and epigallocatechingallate of 0.70 g and 286.87 mg/g dry weight, respectively.


2017 ◽  
Vol 32 (3) ◽  
pp. 295 ◽  
Author(s):  
Silas Da Silva Santos ◽  
Marco Antonio Martin Biaggioni ◽  
Maria Márcia Pereira Sartori ◽  
Iara Maria Casarini Monteiro ◽  
Ivan Fernandes De Souza

A prensagem mecânica é o método mais empregado para extração do óleo de diversos tipos de oleaginosas, constituindo-se em uma operação simples que não exige mão de obra qualificada e facilmente adaptável a diversos tipos de grãos. Entretanto, as prensas contínuas com pequena capacidade, tem um grande potencial de utilização nas pequenas comunidades rurais, apresentam baixa eficiência de extração, deixando uma quantidade significativa de óleo na torta. A operação de retirada das cascas dos frutos de crambe, antes da prensagem, constitui-se em um fator importante que pode otimizar a extração mecânica do óleo de crambe, reduzindo o teor de óleo que se perde pelo processamento do grão com casca. O objetivo deste trabalho foi avaliar o rendimento de óleo de crambe extraído mecanicamente, contendo diferentes percentagens de retirada de casca. Foram realizados os testes de rendimento de extração do óleo pelo método mecânico de prensagem a frio, a partir do crambe submetido aos seguintes tratamentos de descasque: grãos sem casca, grãos com 10% de casca, grãos com 20% de casca e grãos com casca (controle). Para avaliar o efeito do descasque foi utilizado um delineamento experimental inteiramente casualizado, com quatro tratamentos e quatro repetições. Os resultados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Os resultados obtidos neste trabalho permitiram observar o efeito positivo e significativo do descasque dos grãos sobre a extração do óleo de crambe, atingindo o máximo rendimento com a ausência de casca.PALAVRAS-CHAVE: crambe, descasque, rendimento de extração, prensagem mecânica, eficiência de extração. DEHULLING PROCESS OF CRAMBE [Crambe Hyspanica subesp. Abyssinica (Hochst. ex R.  E. Fr.) PRINA] AIMING TO OIL EXTRACTIONABSTRACT: The mechanical screw pressing (expeller) is the method most commonly used for extracting oil from various types of oilseeds, constituting of a simple operation that requires no skilled labor and is easily adaptable to various types of grain. However, continuous presses with small capacity have great potential of use in small rural communities, and have low extraction efficiency, leaving a significant amount of oil in the crambe cake. To remove the hull from crambe fruits before pressing is an important factor that can optimize the mechanical extraction of crambe oil, reducing oil waste. The purpose of this work was to evaluate the yield of mechanically extracted oil from crambe fruits containing different percentages of hull. The oil extraction yield tests were performed by the cold pressing method, submitting the crambe to the following hulling treatments: totally dehulled, partially dehulled with 10% hull, partially dehulled with 20% hull, and whole grains (control). To evaluate the dehulling effect, a completely randomized experimental design was used, with four treatments and four replications.  The results were submitted to analysis of variance and the averages were compared by the Tukey’s test at 5% probability.  The results obtained in this work allowed observing the positive and significant effect of hulling to crambe oil extraction. The absence of hull resulted on maximum yield of oil extracted.KEYWORDS: crambe, dehulling, extraction yield, mechanical pressing, extraction efficiency.


2016 ◽  
Vol 8 (1) ◽  
pp. 405-411 ◽  
Author(s):  
V. Ramya ◽  
Udaykumar Nidoni ◽  
Sharangouda Hiregoudar ◽  
C. T. Ramachandra ◽  
J. Ashoka ◽  
...  

In the present study, supercritical fluid extraction (SFE) technology was applied to extract deoxynojirimycin (1-DNJ) from mulberry leaf powder using carbon dioxide (CO2) as major extraction solvent with ethanol as cosolvent, and extraction parameters such as pressure (100, 150 and 200 bar), temperature (40, 50 and 60 °C) anddynamic extraction time (40, 60 and 80 min) were systematically investigated by full factorial design to obtain the optimum extraction efficiency and extraction yield. Under optimized conditions (pressure of 200 bar, temperature of 50 °C and dynamic extraction time of 80 min), DNJ enriched extract was obtained with high extraction efficiency (96.46 %) and extraction yield (13.41 %), enabling this product to use for nutraceutical purpose. The results indicated that SC-CO2 extraction is a promising and alternative process for recovering the bioactive compounds from mulberry leaves.


2011 ◽  
Vol 1 (1) ◽  
pp. 194-200
Author(s):  
E. R. Gonzáles ◽  
A. L. Klingensmith ◽  
D. S. Peterson

AbstractThis paper presents a radiochemical procedure that combines radiochemical sample preparation and analyte separation to provide a rapid sample preparation method. Extractive ligands are prepared in a polymer matrix. This system can be immobilized on a surface or on a filter. Filters allow rapid sample uptake due to the movement of the analyte through the filter material. These approaches offer fast uptake kinetics, high material loading, and good selectivity. These systems have been demonstrated for the rapid extraction of plutonium and americium from solution and their subsequent analysis by alpha spectrometry, with no additional sample preparation required, thus eliminating extensive wet chemical processes that would otherwise be necessary. We have evaluated the extraction efficiency that can be obtained and have shown that these systems can provide for alpha spectrometry peak widths near what can be obtained using traditional techniques such as electroplating. We have also demonstrated Polymer Ligand Films (PLFs) for the selective extraction of plutonium from a solution prepared from a soil digest.


Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 322 ◽  
Author(s):  
Mircea Oroian ◽  
Florin Ursachi ◽  
Florina Dranca

The aim of this study was to evaluate the extraction efficiency of polyphenols from crude pollen by an ultrasonic process. Prior to the polyphenols extraction, the crude pollen was defatted. The extraction from defatted pollen was carried out by varying four extraction parameters: ultrasonic amplitude (20%, 60% and 100%), solid/liquid ratio (10 g/L, 20 g/L and 30 g/L), temperature (35, 50 and 65 °C) and time (10, 20 and 30 min). The extracts were analyzed in terms of extraction yield (%), total phenolic content (TPC) and total flavones content (TFC). The extracted oil was analyzed in terms of fatty acids composition; myristic acid (159.1 µg × g−1) and cis-14-pentadecenoic acid (106.6 µg·g−1) were found in the highest amount in the pollen oil. The optimum conditions of extraction were determined and were, as follows: 100% amplitude of ultrasonic treatment, 30 g/L solid/liquid ratio, 40.85 °C and 14.30 min, which led to the extraction of 366.1 mg GAE/L of TPC and 592.2 mg QE/g of TFC, and also to an extraction yield of 1.92%.


Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1062 ◽  
Author(s):  
Janet Menzio ◽  
Arianna Binello ◽  
Alessandro Barge ◽  
Giancarlo Cravotto

Natural caffeine from decaffeination processes is widely used by pharmaceutical, cosmetic and soft-drink industries. Supercritical CO2 extraction (SFE–CO2) is extensively exploited industrially, and one of its most representative applications is the decaffeination process, which is a greener alternative to the use of organic solvents. Despite its advantages, extraction kinetics are rather slow near the CO2 critical point, meaning that improvements are highly sought after. The effect exerted by a combination of SFE–CO2 and ultrasound (US–SFE–CO2) has been investigated in this preliminary study, with the aim of improving mass transfer and selectivity in the extraction of caffeine from green coffee beans. This hybrid technology can considerably enhance the extraction efficiency and cut down process time. Further studies are in progress to demonstrate the complete decaffeination of green coffee beans of different types and origins.


2014 ◽  
Vol 563 ◽  
pp. 384-390
Author(s):  
Tang Kai ◽  
Yan Jie ◽  
Huang Xin

In this paper, microwave-surfactant assisted extraction of saponin from Momordica Charantia.L was studied. The content of saponin was determined by the method of vanillin-acetic acid and perchloric which were used as developer. Four main factors including extraction powder, extraction time, ratio of solid to liquid and surfactant concentration were studied by orthogonal experiments. Results showed that extraction powder was the most significant influential factor, followed by extraction time and ratio of solid to liquid. Surfactant concentration showed the minimum influence. The optimum extraction conditions were: extraction powder 400W, extraction time 5min, ratio of solid to liquid 1:12g/mL and surfactant concentration 0.03mol/L, under which the extraction yield of saponin reached 4.69%.


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