Bacteriocin-producing strain of Enterococcus faecium EK 13 with probiotic character and its application in the digestive tract of rabbits

Biologia ◽  
2006 ◽  
Vol 61 (6) ◽  
Author(s):  
Andrea Lauková ◽  
Viola Strompfová ◽  
Viera Skřivanová ◽  
Zdeněk Volek ◽  
Eva Jindřichová ◽  
...  
Keyword(s):  
Drinking Water ◽  
Enterococcus Faecium ◽  
Average Daily Gain ◽  
Standard Diet ◽  
Probiotic Properties ◽  
Resistant Variant ◽  
E Coli ◽  
Significant Difference ◽  
Enterocin A ◽  
Daily Gain

AbstractEnterococcus faecium EK 13 is a bacteriocin-enterocin A producing strain with probiotic properties. In this study its colonization, stability and effect on microflora in rabbits was studied as well as its influence on zootechnical parameters. Fifty rabbits of both sexes (HYPLUS, 30-day old; after weaning) were divided into control (CG) and experimental (EG) groups. They were fed a standard diet. Moreover, 25 rabbits in EG were fed daily (for 4 weeks) 15 g (separate doses ∼1.6 g) of lyophilized EK13 strain (rifampicin resistant variant — rifR; 109 cfu/g) dissolved in drinking water. After cessation of EK13 (rifR) strain application, the rabbits in both groups were fed a standard diet for the next 2 weeks. Sampling was performed in double on day 0 (at the beginning of experiment), weekly during EK13 (rifR) strain application as well as on week 1 and 2 after cessation of EK13 (rifR) strain application. The counts of EK13 (rifR) strain reached 7.1 ± 2.6 log10 cfu/g after 4 weeks and even on week 2 after its cessation the counts 5.6 ± 2.3 log10 cfu/g were determined. The total counts of enterococci in the rabbits were already increased in EG comparing with CG (p < 0.05); even 2 weeks after EK13 (rifR) strain cessation, their counts in EG were 7.2 ± 2.6 log10 cfu/g (p < 0.001). Enterococci in CG reached at the same time the value 3.7 ± 2.6 log10 cfu/g. The counts of E. coli were significantly reduced in EG during 4 weeks (p < 0.05, p < 0.001). Even 2 weeks after EK13 (rifR) strain cessation significant difference in E. coli counts between CG and EG was detected (p < 0.001). Enterobacteria in EG were significantly reduced (p < 0.001). Average daily gain in EG was 41.0 ± 3.83 in comparison to CG (40.6 ± 3.72); it means almost the same; although rabbits in EG showed higher feed intake per kg of gain than rabbits in CG. Preliminary results demonstrated that EK13 is a perspective probiotic candidate for rabbits.

scholarly journals PSIII-5 Effects of ingredient composition of oral drenches in small piglets post-farrowing on average daily gain and pre-wean mortality

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 238-238
Author(s):  
Olivia Harrison ◽  
Samantha Tauer ◽  
Brent Frederick
Keyword(s):  
Birth Weight ◽  
Average Daily Gain ◽  
High Energy ◽  
Experimental Unit ◽  
Swine Industry ◽  
Bioactive Proteins ◽  
Randomized Design ◽  
Significant Difference ◽  
Key Factor ◽  
Daily Gain

Abstract Number of pigs born alive has been a key factor of the increasing efficiency of the U.S. swine industry. However, with increased pigs in the uterus, birth weight has been negatively impacted, with more small or at-risk pigs being born per litter. In order to overcome these changes, a study testing three commercial oral drenches against a control to determine which would increase average daily gain and decrease preweaning mortality. In a completely randomized design, 877 one-day-old suckling pigs from a high-health farm were selected for the experiment if they appeared to be in the bottom 20% of bodyweight compared to their contemporaries. Selected pigs were given one of four drenching treatments: 1) none (control), 2) bioactive proteins (BP), 3) high energy sugars (HES), and 4) immunoglobulins (IgY). Pigs were weighed on d 1 and d 19 of age (weaning), with mortality tracked during the suckling period. Data were analyzed using SAS v 9.4 (Cary, NC), with pig as the experimental unit and an accepted alpha of 0.05. Treatment had no detected effect on birth weight, weaning weight, ADG, or mortality (P = 0.79, 0.96, 0.86, 0.38 respectively). Likewise, statistical contrasts were used to determine there was no detected impact (P &gt; 0.10) of drench, regardless of type, compared to the control in any measured response criteria. Interesting, pigs drenched with BP or IgY had numerically lower preweaning mortality (11.2 and 11.5% respectively), than those administered the control or HES (15.4 and 15.2%, respectively). In conclusion, this experiment showed no significant difference in the performance between piglets given no product vs. those drenched with bioactive proteins, high energy sugars, or immunoglobulins. However, additional research is warranted with greater replication or disease stressors to better understand if oral drenches may improve preweaning performance or mortality in different situations.

scholarly journals THE EFFECTS OF LEVELS OF ISOLATION, OR VARIETAL DIFFERENCES IN, HIGH FIBRE HULL FRACTION OF LOW GLUCOSINOLATE RAPESEED MEALS ON RAT OR PIG PERFORMANCE

1978 ◽  
Vol 58 (4) ◽  
pp. 743-752 ◽  
Author(s):  
J. J. KENNELLY ◽  
F. X. AHERNE ◽  
A. J. LEWIS
Keyword(s):  
Feed Intake ◽  
Average Daily Gain ◽  
Rapeseed Meal ◽  
Sprague Dawley ◽  
Crude Fibre ◽  
Gain Ratio ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Pig Performance ◽  
Daily Gain

Forty-eight crossbred pigs of average initial weight 21 kg were fed 10% Tower rapeseed meal (RSM) and 10% Candle RSM as partial replacements for soybean meal (SBM). Diets were formulated to be isocaloric. Pigs fed the SBM diet consumed less feed, gained significantly (P < 0.01) faster and were more efficient at converting feed to gain than those fed the RSM diets. Performance of pigs fed Candle RSM was not significantly different to that obtained with Tower RSM. In a second experiment, dehulled Tower RSM and Tower RSM hulls were mixed in amounts to produce RSM with crude fibre levels of 6.8, 10.8, 13.5 and 15.8%. The simulated RSM and Tower and Candle RSM were used to completely replace SBM in the diets of weanling (75 g) Sprague-Dawley rats. Rats fed SBM had significantly (P < 0.05) higher average daily gain (ADG) than those fed Tower or Candle RSM, or diets containing the rapeseed meats. There was no significant (P < 0.05) difference in ADG, feed intake or feed to gain ratio of rats fed either Tower or Candle RSM. Feed intake, feed to gain ratio and fecal volatile fatty acid concentrations increased while average daily gain decreased with increasing level of hulls in simulated RSM diets. There was no significant difference (P < 0.05) in thyroid weight between rats fed SBM, Tower RSM or Candle RSM.

scholarly journals Meat productivity of quails by feeding nanocrystalline cerium dioxide

2021 ◽  
pp. 57-64
Author(s):  
V. Zotsenko ◽  
V. Bityutsky ◽  
D. Ostrovskiy ◽  
A. Andriichuk
Keyword(s):  
Drinking Water ◽  
Cerium Dioxide ◽  
Average Daily Gain ◽  
Live Weight ◽  
Feed Additive ◽  
Nanocrystalline Cerium Dioxide ◽  
Meat Productivity ◽  
Daily Gain ◽  
Edible Parts ◽  
Experimental Group

The use of nanomaterials in poultry farming has become increasingly popular as feed additives capable of increasing the productivity of the herd. Nanocrystalline cerium dioxide belongs to nanomaterials with a broad spectrum of activity. Owing to their oxygen non-stoichiometry and low toxicity, cerium dioxide nanoparticles are a very promising object for agriculture. The paper presents the results of studying the meat productivity of quails of the Pharaoh breed when drinking nanocrystalline cerium dioxide in the composition of the feed additive “Nanocerium”. The study was carried out on quails during the rearing period from 14 to 49 days. For the experiment, two groups (control and experimental) of 24 heads each were formed from one day old poultry. The quails were kept in a vivarium, in cage batteries, subject to the accepted microclimate conditions. Poultry of both groups received compound feed, developed taking into account age and physiological characteristics. With drinking water, the of the experimental group additionally received nanocrystalline cerium dioxide as part of the feed additive “Nanocerium” at a dose of 8,6 mg per liter of water for 35 days. The quail population was counted and weighed weekly, starting from the day-old age. The following indicators were determined: safety, dynamics of live weight, average daily gain in live weight. According to the results of the control slaughter and anatomical cutting, the weight of the carcass and the weight of the edible part were determined. Slaughter qualities and morphological composition of quail carcasses were determined by anatomical cutting with the measurement of the following parameters: pre-slaughter weight, half-battered carcass weight, carcass patrana, slaughter yield, weight of edible parts. Drinking the feed additive “Nanocerium” by young quails increased their safety by 4.17%, live weight and absolute average daily gain by 20.3 g and 0.48 g (P <0.05), respectively. The addition of NDC quails to drinking water contributed to an increase in their pre-slaughter weight by 19.3 g, half-gutted carcass by 18.0 g, and gutted by 17.5 g in comparison with the control group. The quail carcasses of the experimental group had a higher yield of edible parts in comparison with the poultry that consumed pure water. Key words: quail, nanocrystalline cerium dioxide, safety, body mass, gain, slaughter rates, output of edible part.

scholarly journals PENAMBAHAN DAUN GEDI (Abelmoschus Manihot (L) Medik) SEBAGAI “ADDITIVE” DALAM AIR MINUM DAN PENGARUHNYA TERHADAP PERFORMANS AYAM KAMPUNG SUPER

ZOOTEC ◽  
2019 ◽  
Vol 39 (1) ◽  
pp. 82
Author(s):  
Desben Kogoya ◽  
J S Mandey ◽  
L J Rumokoy ◽  
M N Regar
Keyword(s):  
Drinking Water ◽  
Water Consumption ◽  
Average Daily Gain ◽  
Nutritional Composition ◽  
Metabolic Energy ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Native Chicken ◽  
Daily Gain

UTILIZATION OF “GEDI LEAF (Abelmoschus Manihot (L) Medik) AS“ADDITIVE” IN DRINKING WATER RELATED TO THE PERFORMANCE OF INDONESIAN SUPER NATIVE CHICKEN. Research was done to evaluate the performance of Indonesian super native chicken treated with “Gedi” leaf (Abelmoschus Manihot (L) Medik) as“Additive” in drinking water. Study was conducted using hundred individuals of the Indonesian super native day old chicken. The juice of “Gedi” leaf (Abelmoschus Manihot (L) Medik) was given as in forms of the four treatments as follows, drinking water without “Gedi” leaf as control (P0), drinking water with 10 ml juice of “Gedi” leaf per liter of drinking water (P1), drinking water with 20 ml juice of “Gedi” leaf per liter of drinking water (P2), and drinking water with 30 ml juice of “Gedi” leaf per liter of drinking water (P3). Treatments were applied to the birds at 6 weeks old by completely randomized design five replications at each treatment. Data were taken during 5 weeks. Feeds were using 73 percents of commercial feed added by 10 percents of corn and 17 percents of rice bran with nutritional composition of crude protein 19.49 percents, crude fiber 4.66 percents, fat 3.63 percents, Calcium 1.02 percents, Phosphorus 0.66 percents and metabolic energy of 2920 kcal/kg, all given ad libitum. Variables measurement were focused on feed consumption, drinking water consumption, average daily gain, and feed conversion. Results showed that drinking water with 30 ml juice of “Gedi” leaf per liter of drinking water (P3) was not significantly different with other treatments for feed and drinking water consumption, except for average daily gain and feed conversion. The treatments of P1 and P2 were not significantly different for average daily gain, but these treatments were higher in average daily gain compared with control (P0). The highest value of feed conversion was obtained at treatment P3 and the lowest value was found at treatment P1. These values indicated that the best feed conversion was treatment P1. Therefore, it can be concluded that juice of “Gedi” leaf can be used as the alternative additive feed in drinking water up to 20 ml juice of “Gedi” leaf per liter of drinking water in term of high average daily gain and feed conversion of Indonesian super native chicken. Key words: Drinking water, “Gedi” leaf, Indonesian super native chicken.

scholarly journals Productive performance of finishing lambs fed with faveleira fodder salt (Cnidoscolus quercifolius Pohl)

2016 ◽  
Vol 37 (2) ◽  
pp. 977
Author(s):  
João Vinícius Barbosa Roberto ◽  
Bonifácio Benicio de Souza ◽  
Gabriel Jorge Carneiro de Oliveira  ◽  
Jaime Miguel de Araujo Filho ◽  
Thaiz Lamy Alves Ribeiro ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Dry Matter ◽  
Salt Intake ◽  
Average Daily Gain ◽  
Daily Intake ◽  
Mineral Salt ◽  
Productive Performance ◽  
Feed Conversion ◽  
Significant Difference ◽  
Daily Gain

This study evaluated food intake and productive performance of crossbred (½ Santa Inês x ½ dorper) lambs supplemented with different levels of faveleira (Cnidoscolus quercifolius Pohl) fodder salt. Thirty male sheep fed tifton grass hay, water, and faveleira fodder salt were randomly allocated into five treatments with six replicates each. The treatments consisted of diets with different inclusion levels of faveleira hay in the fodder salt composition: Treatment 1 (1% mineral salt + 99% faveleira hay), Treatment 2 (3% mineral salt + 97% faveleira hay), Treatment 3 (5% mineral salt + 95% faveleira hay), Treatment 4 (7% mineral salt + 93% faveleira hay), and Treatment 5 (Control - 100% mineral salt). Intake of dry matter, tifton hay and water, average daily gain, feed conversion, and feed efficiency were not affected by fodder salt supplementation (P > 0.05). There was a significant difference (P < 0.05) in fodder salt intake between Treatments 4 and 5, and daily intake was higher in animals submitted to Treatment 4 (61.0 g day-1). Mineral salt intake increased significantly with increasing mineral salt levels in the diet. However, no significant difference was observed in average daily gain across treatments, indicating that faveleira hay, even in small quantities, and tifton hay were able to meet the nutritional requirements of animals to support a good average daily gain. The inclusion of up to 99% faveleira hay in fodder salt formulations did not affect voluntary intake of forage, water and dry matter, average daily gain, feed conversion, and feed efficiency. Lambs supplemented with faveleira fodder salt had average daily gains within the optimal range for slaughter and high feed conversion and feed efficiency values. Faveleira was shown to be an effective supplementary feed alternative in sheep.

Genetic improvement of meat sheep. 1. A comparison of different methods of correcting weaning weight for age of lamb

1976 ◽  
Vol 16 (80) ◽  
pp. 325 ◽  
Author(s):  
IP Gregory ◽  
EM Roberts ◽  
JW James
Keyword(s):  
Birth Weight ◽  
Genetic Improvement ◽  
Average Daily Gain ◽  
Weaning Weight ◽  
Significant Difference ◽  
Meat Sheep ◽  
Daily Gain ◽  
Weight For Age

Several methods of correcting lamb weaning weight for age were compared. Lambs were weighed at birth, at 90 � 1 day of age, when the oldest and when the youngest lamb was 90 days and when the average age of lambs was 90 days. Weights were adjusted to 90 days by the methods of regression, regression pooled within sire groups, average daily gain with and without a standard birth weight and weight per day of age. All the correction methods examined gave similar results and there was no significant difference between methods. The correlations between the actual 90-day weight and the adjusted weights ranged from 0.922 to 0.960 and were highest when the weights taken when the average age of lambs was 90 days were used as the basis for adjustment. All adjusted weights were significantly biased as estimates of actual 90-day weight, but the bias was least for weights when average age was 90 days.

scholarly journals Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs

2009 ◽  
Vol 54 (No. 8) ◽  
pp. 359-364 ◽  
Author(s):  
Z. Vykoukalová ◽  
A. Knoll ◽  
S. Čepica
Keyword(s):  
Linkage Disequilibrium ◽  
Amino Acid ◽  
Association Study ◽  
Gene Structure ◽  
Average Daily Gain ◽  
Large White ◽  
Meat Content ◽  
Significant Difference ◽  
Daily Gain ◽  
I Gene

Perilipin, encoded by the <I>PLIN</I> gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine <I>PLIN</I> gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G>A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan × Piétrain cross. Associations were studied between loci g.4119A>G and g.7966T>C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content (LMC) in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A>G and g.7966T>C only genotype combinations <I>AA-TT, AG-TC</I> and <I>GG-CC</I> were detected. A significant difference (<I>P</I> = 0.0290) between <I>GG-CC</I> and <I>AG-TC</I> genotype combinations for BFT in herd 2 and a suggestive difference (<I>P</I> = 0.0516) between <I>GG-CC</I> and <I>AA-TT</I> genotypes for ADG in herd 1 were detected.

scholarly journals Identification of Escherichiacoli from groups A, B1, B2 and D in drinking water in Brazil

2007 ◽  
Vol 5 (2) ◽  
pp. 323-327 ◽  
Author(s):  
Renato H. Orsi ◽  
Nancy C. Stoppe ◽  
Maria Inês Z. Sato ◽  
Laura M. M. Ottoboni
Keyword(s):  
Drinking Water ◽  
Distribution System ◽  
Water Distribution ◽  
Rapid Assessment ◽  
Waterborne Diseases ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Significant Difference ◽  
Drinking Water Distribution ◽  
Contamination Events

The presence of Escherichia coli in drinking water is an indication of fecal contamination and can represent a risk of waterborne diseases. Forty-nine E.coli strains isolated from different sources of drinking water (distribution system, well, spring and mineral water) were placed into the phylogenetic groups A (15 strains), B1 (19 strains), B2 (2 strains) and D (13 strains). Approximately 30% of the strains analyzed belonged to groups B2 and D, which usually include potentially extraintestinal pathogenic strains. Moreover, the assignment of the strains to different phylogenetic groups indicates that different contamination events occurred in these waters. These results were compared with the distribution of E. coli strains isolated from two rivers and two dams into the phylogenetic groups. A significant difference was observed when the distribution of drinking water strains into the phylogenetic groups was compared to the results obtained from the Guarapiranga Dam and the Jaguari and Sorocaba Rivers. The results obtained in this work suggest that PCR-based methods can be used for a rapid assessment of potentially pathogenic E. coli strains in water samples.

Influence of early weaning on the behavioural patterns and growth performance in large white Yorkshire (LWY) piglets

2016 ◽  
Author(s):  
V. Sravanthi ◽  
D. Suresh Babu ◽  
A. Anitha ◽  
P. Asha Latha
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Early Weaning ◽  
Large White ◽  
Significant Difference ◽  
Weaning Age ◽  
Group 3 ◽  
Group 2 ◽  
Daily Gain ◽  
Group 1

The behavioural pattern and growth performance of three groups of LWY piglets weaned at different ages was studied for six months. The first, second and third groups were weaned at 25, 40 and 56 days, respectively. The playing and huddling behavior was more in group 1 piglets. While fighting, standing, locomotion and tail biting behavior was more in group 2 piglets. Whereas the lying behavior was more in group 3 piglets. There was a significant difference (P is less than 0.01) among the three groups of piglets for all behavioral patterns. The post weaning weight (kg) gains reached 56.63 ± 0.41, 65.03 ± 0.36 and 63.09 ± 0.38 Kg for group 1, group 2 and group 3 respectively, at the end of study period. A significant difference (P is less than 0.01) was observed among the three groups for all the fortnightly interval body weights. The mean average daily gain for group 1, 2 and 3 was 0.33, 0.38 and 0.36 respectively. Significant difference (P is less than 0.01) had been observed between the groups for average daily gain. The present study had suggested that early weaning at 40days age was more advantageous than 25 days age and conventional 56days weaning age.

scholarly journals INFLUENCE OF PORCINE IMMUNOGLOBULIN ADMINISTRATION ON SURVIVAL AND GROWTH OF PIGS WEANED AT TWO AND THREE WEEKS OF AGE

1979 ◽  
Vol 59 (4) ◽  
pp. 693-698 ◽  
Author(s):  
J. J. KENNELLY ◽  
R. O. BALL ◽  
F. X. AHERNE
Keyword(s):  
Average Daily Gain ◽  
Treated Group ◽  
Control Group ◽  
Weaned Piglets ◽  
Stomach Tube ◽  
E Coli ◽  
Porcine Serum ◽  
Neomycin Sulfate ◽  
Survival And Growth ◽  
Daily Gain

Three experiments were conducted to study growth, incidence of scouring and mortality of 2- and 3-wk weaned piglets fed immunoglobulins (IMG) derived from abbatoir porcine serum. In experiment 1, 20 piglets weaned at 2 wk of age were randomly divided into a control and an IMG-treated group. In both groups 1 × 1012 organisms of a pathogenic strain of Escherichia coli was administered by stomach tube. The IMG-treated group had a faster (P < 0.01) average daily gain, reduced incidence of scouring (P < 0.01) and lower mortality (P < 0.07) than the control group. In experiment 2, 36 piglets weaned at 3 wk of age were allotted to a control and IMG-treated group. In the absence of a loading dose of E. coli there were no significant differences in average daily gain, scour scores or rectal temperature between the control and the IMG-treated groups. In the third experiment, 72 piglets were weaned at 3 wk of age and randomly allotted to three treatments: a control, an IMG-treated and an antibiotic-treated (Neomycin Sulfate) group. The results of the former two groups were similar to those obtained in experiment 2. The antibiotic-treated group had a faster (P < 0.01) gain than both control and IMG group despite the similarity in the incidence of scouring.

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