Carotenoids, ergosterol and tocopherols in fresh and preserved herbage and their transfer to bovine milk fat and adipose tissues: A review

2012 ◽  
Vol 29 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Pavel Kalač
Keyword(s):  
Milk Fat ◽  
Bovine Milk ◽  
Alpha Tocopherol ◽  
Maize Silage ◽  
Forage Crops ◽  
Adipose Tissues ◽  
Factors Affecting ◽  
Preservation Method ◽  
Fat Soluble Vitamins ◽  
Β Carotene

Abstract The requirements of cattle for the fat-soluble vitamins A, D and E, their provitamins and some carotenoids have increased under conditions of intensive husbandry with insufficient provision of fresh forage, their cheapest natural source. Moreover, bovine milk fat and adipose tissues participate in the intake of these micronutrients by humans. Four major carotenoids occurring in forage crops are lutein, all-trans-β-carotene, zeaxanthin and epilutein. Fresh forage is their richest source. Losses are significantly higher in hay as compared with silage, particularly if prepared from unwilted herbage. Maize silage is a poor source of carotenoids as compared with ensiled grasses and legumes. Ergosterol contents in forages increase under environmental conditions favourable for the growing of moulds, particularly at higher humidity and lower temperatures. Credible data on changes of ergosterol during herbage preservation, particularly drying and ensiling, have been lacking. Alpha-tocopherol is the most important among eight related compounds marked as vitamin E. It is vulnerable to oxidation and herbage ensiling is thus a safer preservation method than haymaking. As with carotenoids, maize silage is a poor source of α-tocopherol. Overall, information on factors affecting the content of ergosterol and tocopherols in fresh herbage, on changes during forage preservation and on transfer to bovine fats has been much more limited than data for β-carotene.

scholarly journals The effect of feeding diets markedly differing in the proportion of grass and maize silages on bovine milk fat composition

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 93-100 ◽  
Author(s):  
E. Samková ◽  
M. Pešek ◽  
J. Špička ◽  
T. Pelikánová ◽  
O. Hanuš
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Saturated Fatty Acids ◽  
Bovine Milk ◽  
Atherogenic Index ◽  
Maize Silage ◽  
Grass Silage ◽  
Lactation Stage ◽  
Fat Composition ◽  
The Individual

Ten Czech Pied cows in the mid-lactation stage were fed diets based on grass silage and maize silage. The composition of milk fats differed. The proportions of even-chain saturated fatty acids (SFAs) up to C<sub>14:0</sub> were insignificant and the content of C<sub>16:0</sub> was significantly higher (<i>P</i> < 0.05) when feeding a diet based on maize silage, while the proportions of the individual polyunsaturated fatty acids (PUFAs) were significantly (except for C<sub>18:2</sub>) higher when feeding a diet based on grass silage. The total SFA proportions were 67.60 and 62.93% (<i>P</i> < 0.05) of maize and grass silages, respectively, while an opposite relation was observed for the sum of PUFAs (3.56 and 4.74%; <i>P</i> < 0.001). Feeding of grass silage resulted in a significantly lower proportion of hypercholesterolaemic fatty acids C<sub>12:0</sub>, C<sub>14:0</sub> and C<sub>16:0</sub> (49.38 and 44.98%, respectively; <i>P</i> < 0.05) and in lower values of the atherogenic index (3.03 and 2.44; <i>P</i> < 0.05). Thus, the results could be used for the improvement of milk fat composition.

Fatty acid content in bovine milk under the action of β-carotene

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
L Shevchenko ◽  
◽  
V Mykhalska ◽  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Vitamin A ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Bovine Milk ◽  
Feed Additives ◽  
The Body ◽  
Biologically Active ◽  
Β Carotene

One of the key issues in the production of high-quality, biologically complete milk is to provide cows with a sufficient amount of biologically active substances, including vitamin A and its precursor β-carotene. One of the ways to enrich milk with vitamin A and carotenoids is feeding cows with natural feed additives, including vitaton containing up to 10% trans-β-carotene, which can be converted into vitamin A, as well as exhibit antioxidant, immunostimulating, and anticarcinogenic effects in the body. Vitaton is the biomass of fungi Blakeslea trispora TKST strain, a product of microbial synthesis obtained by cultivating the producer on the waste of starch and molasses production. In terms of sanitation, the biomass of the fungi Bl. trispora is safe for animals, since the main condition for its production is sterility, excluding any microorganisms entering the fermentation medium. Besides, after the end of fermentation, the temperature of the medium is raised to 65–70 °C in the fermenter that destroys the β-carotene producer itself. We conducted research on cows of the Ukrainian black and white dairy breed, which were fed with vitaton, in conditions of “Kuibysheve” ALLC of the Poltava region. The introduction of vitaton into the cows’ diet contributes to the increase of the milk fat content by an average of 0.26% and enhances the phospholipid synthesis in the mammary gland by 1.8 times. Vitaton as a biologically active additive did not affect the ratio of total saturated to unsaturated fatty acids in bovine milk but stimulated the formation and inclusion of two unsaturated fatty acids into the milk fat, namely nonadecanoic and α-linolenic, against the background of the disappearance of the arachidonic acid peak in the chromatogram. Changes in the fatty acid composition of milk fat obtained from cows fed with the vitaton as a source of β-carotene indicate the effect of β-carotene and other biologically active components contained in vitaton on milk lipid synthesis.

Maize silage and winter crop options to maximise drymatter and energy for NZ dairy systems

2006 ◽  
pp. 193-197 ◽  
Author(s):  
R.J. Densley ◽  
G.M. Austin ◽  
I.D. Williams ◽  
R. Tsimba ◽  
G.O. Edmeades
Keyword(s):  
Italian Ryegrass ◽  
Maize Silage ◽  
Forage Crops ◽  
Forage Crop ◽  
Maize Hybrid ◽  
Trade Offs ◽  
Feed Value ◽  
Winter Crop ◽  
Winter Crops ◽  
Silage Production

Trade-offs in dry matter (DM) and metabolisable energy (ME) between combinations of three maize silage hybrids varying in maturity from 100-113 CRM and six winter forage options were investigated in a Waikato farmer's field over 2 years. Winter crops were triticale, cut once; oats grazed 1-2 times; and Tama and Feast II Italian ryegrass, each cut or grazed 2-3 times. Greatest DM and ME production (38.9 t/ha; 396 GJ/ha) was from a 113 CRM hybrid followed by a single-cut triticale crop. The most economical sources of DM and ME were obtained from a 100 CRM maize hybrid plus grazed oats (11.8 c/ kg; 1.12 c/MJ), while the cheapest ME source among cut winter forages was a 113 CRM maize hybrid + triticale (1.18 c/MJ). Reliable annual silage production of 30 t DM/ha and 330 GJ ME/ha (or 3000 kg MS/ha) is possible using a late maturing maize hybrid combined with a winter forage crop such as triticale, although the low feed value of the triticale may limit its use as feed for milking cows. Keywords: Italian ryegrass, oats, maize silage, supplements, triticale, winter forage crops

scholarly journals Bovine Milk-Derived Emulsifiers Increase Triglyceride Absorption in Newborn Formula-Fed Pigs

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 410 ◽  
Author(s):  
Kristine Bach Korsholm Knudsen ◽  
Christine Heerup ◽  
Tine Røngaard Stange Jensen ◽  
Xiaolu Geng ◽  
Nikolaj Drachmann ◽  
...  
Keyword(s):  
Milk Fat ◽  
Bovine Milk ◽  
In Vitro Digestion ◽  
Lipid Absorption ◽  
Lipid Digestion ◽  
Soy Lecithin ◽  
Neonatal Piglets ◽  
Milk Fat Globule Membranes

Efficient lipid digestion in formula-fed infants is required to ensure the availability of fatty acids for normal organ development. Previous studies suggest that the efficiency of lipid digestion may depend on whether lipids are emulsified with soy lecithin or fractions derived from bovine milk. This study, therefore, aimed to determine whether emulsification with bovine milk-derived emulsifiers or soy lecithin (SL) influenced lipid digestion in vitro and in vivo. Lipid digestibility was determined in vitro in oil-in-water emulsions using four different milk-derived emulsifiers or SL, and the ultrastructural appearance of the emulsions was assessed using electron microscopy. Subsequently, selected emulsions were added to a base diet and fed to preterm neonatal piglets. Initially, preterm pigs equipped with an ileostomy were fed experimental formulas for seven days and stoma output was collected quantitatively. Next, lipid absorption kinetics was studied in preterm pigs given pure emulsions. Finally, complete formulas with different emulsions were fed for four days, and the post-bolus plasma triglyceride level was determined. Milk-derived emulsifiers (containing protein and phospholipids from milk fat globule membranes and extracellular vesicles) showed increased effects on fat digestion compared to SL in an in vitro digestion model. Further, milk-derived emulsifiers significantly increased the digestion of triglyceride in the preterm piglet model compared with SL. Ultra-structural images indicated a more regular and smooth surface of fat droplets emulsified with milk-derived emulsifiers relative to SL. We conclude that, relative to SL, milk-derived emulsifiers lead to a different surface ultrastructure on the lipid droplets, and increase lipid digestion.

scholarly journals Fatty Acid Prediction in Bovine Milk by Attenuated Total Reflection Infrared Spectroscopy after Solvent-Free Lipid Separation

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1054
Author(s):  
Christopher Akhgar ◽  
Vanessa Nürnberger ◽  
Marlene Nadvornik ◽  
Margit Velik ◽  
Andreas Schwaighofer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Milk Fat ◽  
Regression Models ◽  
Bovine Milk ◽  
Attenuated Total Reflection ◽  
Solvent Free ◽  
Novel Approach ◽  
Whole Milk ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

In the present study, a novel approach for mid-infrared (IR)-based prediction of bovine milk fatty acid composition is introduced. A rapid, solvent-free, two-step centrifugation method was applied in order to obtain representative milk fat fractions. IR spectra of pure milk lipids were recorded with attenuated total reflection Fourier-transform infrared (ATR-FT-IR) spectroscopy. Comparison to the IR transmission spectra of whole milk revealed a higher amount of significant spectral information for fatty acid analysis. Partial least squares (PLS) regression models were calculated to relate the IR spectra to gas chromatography/mass spectrometry (GC/MS) reference values, providing particularly good predictions for fatty acid sum parameters as well as for the following individual fatty acids: C10:0 (R2P = 0.99), C12:0 (R2P = 0.97), C14:0 (R2P = 0.88), C16:0 (R2P = 0.81), C18:0 (R2P = 0.93), and C18:1cis (R2P = 0.95). The IR wavenumber ranges for the individual regression models were optimized and validated by calculation of the PLS selectivity ratio. Based on a set of 45 milk samples, the obtained PLS figures of merit are significantly better than those reported in literature using whole milk transmission spectra and larger datasets. In this context, direct IR measurement of the milk fat fraction inherently eliminates covariation structures between fatty acids and total fat content, which poses a common problem in IR-based milk fat profiling. The combination of solvent-free lipid separation and ATR-FT-IR spectroscopy represents a novel approach for fast fatty acid prediction, with the potential for high-throughput application in routine lab operation.

Production and characterization of monoclonal antibodies directed against bovine milk fat globule membrane (MFGM)

1994 ◽  
Vol 1199 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Naohito Aoki ◽  
Hidenori Kuroda ◽  
Miho Urabe ◽  
Yoshimi Taniguchi ◽  
Takahiro Adachi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Milk Fat ◽  
Bovine Milk ◽  
Milk Fat Globule Membrane ◽  
Milk Fat Globule ◽  
Fat Globule

Chemical changes in bovine milk fat globule membrane caused by heat treatment and homogenization of whole milk

2002 ◽  
Vol 69 (4) ◽  
pp. 555-567 ◽  
Author(s):  
SUNG JE LEE ◽  
JOHN W. SHERBON
Keyword(s):  
Heat Treatment ◽  
Membrane Proteins ◽  
Milk Fat ◽  
Whey Proteins ◽  
Bovine Milk ◽  
Chemical Changes ◽  
Milk Fat Globule Membrane ◽  
Whole Milk ◽  
Milk Fat Globule ◽  
Fat Globule

The effects of heat treatment and homogenization of whole milk on chemical changes in the milk fat globule membrane (MFGM) were investigated. Heating at 80 °C for 3–18 min caused an incorporation of whey proteins, especially β-lactoglobulin (β-lg), into MFGM, thus increasing the protein content of the membrane and decreasing the lipid. SDS-PAGE showed that membrane glycoproteins, such as PAS-6 and PAS-7, had disappeared or were weakly stained in the gel due to heating of the milk. Heating also decreased free sulphydryl (SH) groups in the MFGM and increased disulphide (SS) groups, suggesting that incorporation of β-lg might be due to association with membrane proteins via disulphide bonds. In contrast, homogenization caused an adsorption of caseins to the MFGM but no binding of whey proteins to the MFGM without heating. Binding of caseins and whey proteins and loss of membrane proteins were not significantly different between milk samples that were homogenized before and after heating. Viscosity of whole milk was increased when milk was treated with both homogenization and heating.

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