scholarly journals ANALISIS POTENSI ANTIHIPERKOLESTEROLEMIA EKSTRAK ETANOL BUAH ASAM JAWA (Tamarindus indica L) TERHADAP STRUKTUR MIKROSKOPIS HATI MENCIT (Mus musculus)

2018 ◽  
Vol 18 (2) ◽  
pp. 86-92
Author(s):  
Ervina Dewi ◽  
Fadliyani Fadliyani ◽  
Ismiranda Ismiranda
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Block Design ◽  
Ethanol Extract ◽  
Normal Diet ◽  
High Cholesterol Diet ◽  
Microscopic Structure ◽  
Randomized Block Design ◽  
Structure Observation

Abstrak. Penelitian ini bertujuan untuk mengetahui potensi antihiperkolesterolemia ekstrak etanol buah asam jawa terhadap struktur mikroskopis hati mencit. Rancangan yang digunakan adalah Rancangan Acak Lengkap, terdiri atas 6 perlakuan dan 4 ulangan. Perlakuan terdiri atas Pemberian pakan standard dan akuades (P0), pakan aterogenik dan Aquades (P1), pakan aterogenik dan simvastatin 10 mg (P2), pakan aterogenik dan ekstrak asam jawa 5, 25 dan 50 mg/kg bb (P3, P4, P5). Volume simvastatin dan ekstrak etanol buah asam jawa adalah 0,5mL. Pembuatan sediaan histologis menggunakan metode parafin. Parameter yang diamati adalah kadar kolesterol total, infiltrasi lemak, degenerasi lemak dan nekrosa hepatosit. Data hasil penelitian dianalisis dengan analisis varian dan dilanjutkan dengan Uji Berjarak ganda Duncan. Hasil penelitian menunjukkan ekstrak etanol buah asam jawa berpengaruh nyata dalam menurunkan rerata infiltrasi lemak, degenerasi lemak dan nekrosa sel hati yan diindikasikan dengan penurunan kadar kolesterol secara bermakna. Kesimpulannya,  ekstrak etanol buah asam jawa mampu menurunkan rerata infiltrasi lemak, degenerasi lemak dan nekrosa sel hati mencit akibat pakan aterogenikKata Kunci : Pakan Aterogenik, Hiperkolesterolemia, Hati, Ekstrak Etanol Buah Asam Jawa, SimvastatinAbstract. This research aimed to determine  the potential  antihypercholesterolemic of ethanol extract of tamarind  at heart microscopic structure of mice induced with high cholesterol diet. A completely randomized-block design was used with 6 treatments and 4 repetitions of eachtreatment. The treatments were normal diet and aquadest (P0), hight-fat diet and aquadest (P1), hight-fat diet and Simvastatin 10 mg/Kg (P2), hight-fat diet and tamarind extract 5, 25 and 50 mg/Kg (P3, P4, P5). The volume of Simvastatin and tamarind extract given to each mice is 0,5 mL. Paraffin method was apllied of microscopic structure observation. The parameters observed were  total cholesterol level, lipid infiltration, lipid degeneration and necrosis of hepatocyte of hepatocyte. A variance analysis followed by Duncan Multiple Range Test was tested to the data mice microscopic structure. The result showed that etnanol extract of tamarind could significant to decrease of lipid infiltration, lipid degeneration and necrosis of hepatocyte showed decrease total cholesterol level. Inconlusion, the that etnanol extract of tamarind  could significant to  decrease of lipid infiltration, lipid degeneration and necrosis of hepatocyte due to hight-fat diet.Key Word : Atherogenic Feed, Hypercholesterolemia, Liver, Ethanol Extract of Tamarind, Simvastatin

scholarly journals PENGARUH PEMBERIAN KEFIR SUSU SAPI TERHADAP KADAR KOLESTEROL TOTAL TIKUS JANTAN SPRAGUE DAWLEY

2012 ◽  
Vol 1 (1) ◽  
pp. 224-228
Author(s):  
Finta Wahdania ◽  
Adriyan Pramono
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Serum Cholesterol ◽  
Serum Cholesterol Level ◽  
Total Cholesterol Level ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Cholesterol Lowering

Background : A high serum cholesterol level might become one of contributing risk factor for development of cardiovascular disease (CVD). Serum cholesterol level could decreased by acid lactic bacteria (ALB) content. Kefir, one of probiotic product have been claimed as a cholesterol-lowering agent. The purpose of this research was to determine effect of cwo milk kefir to serum total cholesterol level in animal model. Method : true-experimental by using pre post test with randomized control group design. Twenty eight male Sprague dawley rat were divided into four group (K,P1, P2, P3) and induced by high cholesterol diet. Intervention started from 1,5 ml; 2 ml and 3 ml of kefir respectively to P1, P2 and P3 for fifteen days. Cholesterol level was examined by CHOD-PAP method. Data was examined by using one way ANOVA. Result : Total cholesterol level decreased significantly in all group but there is no defference between group. The highest reduction of total cholesterol was 31,45%, found in group P3 followed by 31,40% for K; 30,90% for P2 and 27,04 % for P1. Conclusion : kefir administration did not contribute in lowered total cholesterol level, result of this study did not support administration of kefir as a cholesterol-lowering agent

scholarly journals Uji Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Notika (Arcboldiodendron calosericeum Kobuski) Terhadap Kadar Kolesterol Total Tikus (Rattus norvegicus) Jantan Galur Wistar

2019 ◽  
Vol 2 (1) ◽  
pp. 1-10
Author(s):  
Nuralifah Nuralifah ◽  
Wahyuni Wahyuni ◽  
Parawansah Parawansah ◽  
Ulan Dwi Shintia
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Blood Lipids ◽  
Total Cholesterol Level ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antioxidant Compounds ◽  
Cholesterol Levels ◽  
Fat Consumption

Hyperlipidemia is an increase of lipid due to excessive fat consumption, that the intake and alteration of lipid is not balanced. Notika are plants that contain secondary metabolites in the form of flavonoids, tannins, saponins and triterpenoids which are antioxidant compounds that are thought to have the effect of reducing blood lipids. This study aims to determine the effect of ethanol extract of leaves of Notika on decreasing total cholesterol levels in rats induced by egg and propylthiouracil (PTU). This research was experimental in 6 groups, namely 4 groups given ethanol extract of leaves of Notika at a dose of 50 mg/kgBB, 100 mg/kgBB, 200 mg/kgBB, and 300 mg/kgBB, one group was given Na CMC 0.5% as a negative control, and one group was given simvastatin 10 mg as a positive control. The results showed that the ethanol extract of the leaves of Notika significantly reduced the total cholesterol level of rats with a significant value 0.05 and 300 mg/kgBB is an effective dose to reduce total cholesterol level.

scholarly journals PEMBERIAN EKSTRAK ETANOL BUAH BUNCIS (Phaseolus vulgaris L.) UNTUK MENURUNKAN KOLESTEROL TOTAL, Low Density Lipoprotein (LDL) DAN MENINGKATKAN High Density Lipoprotein (HDL) PADA TIKUS WISTAR DIET TINGGI LEMAK

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Sri Wahjuni ◽  
Ni Luh Rustini ◽  
Putu Yuliantari
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Density Lipoprotein ◽  
Ethanol Extract ◽  
Hdl Cholesterol ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Levels

Excessive fat consumption can increase blood cholesterol level. Phytosterol composition in the plant can decrease blood cholesterol level. One of the plants that contain phytosterol is beans. This study was to prove the effect of antihypercholesterol the ethanol extract of the beans (Phaseolus vulgaris L.) with variety of doses of 50 mg/Kg BW; 100 mg/Kg BW and 150 mg/Kg BW in male Wistar rats with high fat level condition. Subjects of this study were 24 Wistar rats divided into 6 groups randomly with posttest control group study design. First group as negative control, second group as positive control, and the third (high cholesterol diet and ethanol extract in dose of 50 mg/kg,bw), fourth ( high cholesterol diet and ethanol extractin dose of 100 mg/kg,bw), fifth ( high cholesterol diet and ethanol extract in dose of 150 mg/kg,bw),  sixth (high cholesterol diet and simvastatin drug). The data was analyzed with ANOVA Tukey test. The result of this study shows total cholesterol levels decrease 23,88%; 30,14%; 35,82%. Cholesterol LDL levels decrease 38,09%; 52,38%; 61,35%. Cholesterol HDL levels increase 21,86%; 56,56%; 63,84%. Dose variant on this study giving the best result to decrease total cholesterol levels and LDL was 150 mg/Kg BW. Furthermore, 100 mg/Kg BW dose gave the best result to increase HDL cholesterol level. Based on the results it can be suggested that beans ethanol extract is able to decrease total cholesterol level, LDL and increase HDL cholesterol levels.  

scholarly journals Effectiveness of Ficus Elastica Roxb. Ex Hornem Leaf Extract in Reducing Total Cholesterol Level in High Fat Induced Diet Wistar Male Rats

2020 ◽  
Author(s):  
Muhamad Seto Sudirman ◽  
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Ethanol Extract ◽  
Study Groups ◽  
Male Rats ◽  
Plant Metabolites ◽  
Quasi Experimental ◽  
Mean Differences ◽  
Biology Laboratory

ABSTRACT Background: Kebo rubber leaves (ficus elastica roxb) contain flavonoids, polyphenols, and tannins. Flavonoids in the leaves of ficus elastica roxb such as catechins, isoflavones are polyphenolic antioxidants from plant metabolites. The leaves of ficus elastica roxb are trusted and proven empirically in the community to reduce cholesterol levels in the blood. Mice choose animals because they are considered to have physiological similarities with humans. This study aimed to determine the effect of ethanol extract of ficus elastica roxb leaves on reducing total cholesterol level in male Swiss Webster mice. Subjects and Method: This was a quasi-experimental study conducted at Biology Laboratory of the Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung from April to June, 2017. A sample of 25 male Swiss Webster mice was selected at random and allocated into groups. The dependent variable was total cholesterol. The independent variable was the extract of ficus elastica rox. The data were tested by One-Way ANOVA. Result: There were statistically significant mean differences among the study groups (p= 0.002), indicating the effect of ethanol extract of Ficus Elastica Roxb leaves on reducing total cholesterol level in male Swiss Webster mice at various doses. Conclusion: There are statistically significant mean differences among the study groups, indicating the effect of ethanol extract of Ficus Elastica Roxb leaves on reducing total cholesterol level in male Swiss Webster mice at various doses. Keyword: Ethanol extract of Ficus Elastica Roxb leaves, total cholesterol, mice Correspondence: Muhamad Seto Sudirman. School of Health Polytechnic, Pangkalpinang. Email: [email protected] DOI: https://doi.org/10.26911/the7thicph.05.10

Pengaruh Pemberian Terapi Oksigen Hiperbarik Terhadap Kadar Kolesterol Total Pada Tikus Putih (Rattus novergicus) Jantan Galur Wistar Yang Di Induksi Diet Tinggi Lemak

2018 ◽  
Vol 16 (1) ◽  
pp. 1 ◽  
Author(s):  
AZRUL HILDAN SAFRIZAL
Keyword(s):  
Cardiovascular Disease ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
High Fat Diet ◽  
Oxygen Therapy ◽  
Total Cholesterol Level ◽  
High Fat ◽  
Cholesterol Levels

<p>The pattern and lifestyle of today's society with the presence of an interner facility makes people spend more time sitting out than on exercise and increased consumption of high-fat foods may increase the risk of cardiovascular disease. An effective therapy is needed in preventing the occurrence of cardiovascular disease. Hyperbaric oxygen now starts to develop for the treatment of several diseases, which in turn can increase the gene forming antioxidant enzymes and ROS. To determine effect of hyperbaric oxygen therapy on total cholesterol levels of wistar white rats (Rattusnovergicus) induced bye high fat. The study was carried out in an expeative post test only group control of three groups. One group is given standard feed. All groups induced high-fat diet and standard feed. Of the two groups induced, one group was given hyperbaric oxygen therapy with a dose of 3 x 30 minutes for six days on day 7 at a blood test to determine total cholesterol levels<strong>. </strong>One way Anova parametric statistic test showed that p = 0.007 &lt; α proved hypothesis that hyperbaric oxygen therapy giving effect to total cholesterol level of white mice of jantangalurist rings induced by high fat diet. Total cholesterol was significantly different between K (-) and K (+) and between K (-) and P. It was found that hyperbaric oxygen therapy had an effect on total cholesterol level dose of 3x30 minutes for six days.</p>

Tracking of Serum Cholesterol Levels in a Multiracial Sample of Preschool Children

PEDIATRICS ◽  
1992 ◽  
Vol 90 (1) ◽  
pp. 80-86 ◽  
Author(s):  
David S. Freedman ◽  
Tim Byers ◽  
Karen Sell ◽  
Sarah Kuester ◽  
Eva Newe1l ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Initial Level ◽  
Total Cholesterol Level ◽  
Relative Weight ◽  
Cholesterol Levels ◽  
Final Measurement ◽  
Subsequent Measurement ◽  
Initial Measurement

The relation of an initial measurement of serum total cholesterol to subsequent levels over a (mean) 13-month interval was examined in a multiracial (white, Hispanic, American Indian, and black) sample of 1680 one- to four-year-olds. Although the relation of the initial level to the final measurement (r = .54) did not vary by race, sex, relative weight, or changes in relative weight, the association increased with age at the time of the initial measurement (eg, r .64 among 4-year-olds). Based on the initial and final total cholesterol determinations, the within-person standard deviation was 21 mg/dL and the coefficient of variation was 13%. Although the final total cholesterol level was within 5 mg/dL of the initial level for 18% of the children, the two determinations differed by ≥25 mg/dL for about 35% of the children and by ≥50 mg/dL for about 8%. Of the 149 children who had an initial cholesterol level ≥200 mg/dL, 34% (about five times the expected number) had a follow-up level that was similarly elevated whereas 25% had a subsequent measurement below 170 mg/dL. The results indicate that although an initial cholesterol level in early life is moderately predictive of subsequent levels, it may be difficult to interpret a single total cholesterol determination because of substantial within-person variability.

scholarly journals Diabetic retinopathy with or without clinically significant macular edema: The influencing factors

2016 ◽  
Vol 7 (2) ◽  
pp. 142-147
Author(s):  
Barsha Suwal ◽  
Jeevan Kumar Shrestha ◽  
Sagun Narayan Joshi ◽  
Ananda Kumar Sharma
Keyword(s):  
Diabetic Retinopathy ◽  
Glycemic Control ◽  
Macular Edema ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Density Lipoprotein ◽  
Diabetic Patients ◽  
Clinically Significant Macular Edema ◽  
Clinically Significant

Introduction: Diabetic retinopathy is the commonest micro vascular complication in patients with diabetes and remains a leading cause of blindness in people of working age group. Objective: to determine the prevalence of clinically significant macular edema (CSME) and the influence of systemic risk factors Materials and methods: It is a hospital based comparative study conducted in 220 eyes of 110 diabetic patients. DR was graded according to International Clinical Diabetic Retinopathy Severity Scale and CSME was defined according to Early Treatment Diabetic Retinopathy Study (ETDRS) system. The patients were grouped as 1) CSME group (DR and CSME in one or both eyes) and 2) Non- CSME group(CSME in none of the eyes but with any grade of DR).Level of glycosylated hemoglobin (HbA1C), serum total cholesterol, triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and urine for albumin were studied in both groups. Results: CSME was present in 36% of 110 patients. Poor glycemic control and high total cholesterol level showed positive association with CSME (p<0.05). LDL and TG levels were higher and HDL lower in CSME group. However, no statistical significance was found. Conclusion: The CSME is significantly associated with poorer glycemic control and elevated total cholesterol level.

scholarly journals Clinical Investigations of the Effect of Citrus unshiu Peel Pellet on Obesity and Lipid Profile

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Seongil Kang ◽  
Sangyeol Song ◽  
Joosang Lee ◽  
Hyekyung Chang ◽  
Sanghun Lee
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Weight Control ◽  
Large Scale ◽  
Total Cholesterol Level ◽  
Experimental Studies ◽  
Density Lipoprotein ◽  
Citrus Unshiu ◽  
The Impact

Objectives. Several experimental studies have reported antiobesity and lipid-improving effects of Citrus unshiu. However, clinical studies on its effects are lacking. This study was designed to evaluate the impact of Citrus unshiu peel pellet (CUPP) on obesity and lipid profile. Methods. For 118 patients with body mass index (BMI) > 23 who took Citrus unshiu peel pellet (CUPP) for 4 weeks in a Public Health Center, laboratory and biometric readings before and after CUPP administration were analyzed. Results. Mean age of these subjects was 53.8±10.6 years (range: 18-75 years). There were 88 (74.6%) females in the study sample (n = 118). A significant (p < 0.01) decrease in BMI from 27.47±2.24 to 27.27±2.22 was observed in all subjects after CUPP treatment and 65.3% (N = 77) of them lost 1.03±0.83 kg of weight after 4 weeks of treatment. Total cholesterol level was significantly (p < 0.01) decreased from 204.0±37.4 mg/dL to 193.5±36.5 mg/dL. Significant (p < 0.05) decreases in levels of low-density lipoprotein, cholesterol, and triglyceride were also observed. Conclusions. These results suggest that CUPP in practice could help weight control and improve total cholesterol level. Findings of this study provide clinical foundation for future large-scale trials to establish clinical benefits of CUPP.

scholarly journals Familial hypercholesterolemia: etiopathogenesis, diagnosis, treatment and state of the problem in Ukraine

2019 ◽  
Vol 26 (4) ◽  
pp. 23-31
Author(s):  
O. I. Mitchenko ◽  
V. Y. Romanov ◽  
N. M. Chulaevska ◽  
K. O. Timokhova
Keyword(s):  
Total Cholesterol ◽  
Familial Hypercholesterolemia ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Genetic Disorders ◽  
Low Density Lipoprotein ◽  
Coronary Vessels ◽  
Density Lipoprotein ◽  
Epidemiological Data ◽  
Membrane Receptors

Familial hypercholesterolemia (FH) is one of the most common, inherited autosomal dominant diseases. Most often, FH is caused by dominant mutation of the gene, responsible for the synthesis of low density lipoprotein (LDL) membrane receptors that remove LDL from the blood plasma. As a result, individuals with a mutation of this gene from birth have a significantly increased level of cholesterol LDL in the blood. FH mediates the accelerated development of cardiovascular disease of atherosclerotic genesis, especially coronary heart disease (CHD), so the level of cardiovascular mortality in the population of such patients is extremely high. The article focuses on the fact that the main threat of these lipid disorders is the early and rapid initiation of atherosclerotic lesions of coronary vessels: in patients with heterozygous FH with a total cholesterol level of 8–15 mmol/l, CHD usually manifests up to 55 and 60 years, whereas in homozygous patients with a total cholesterol level of 12–30 mmol/l, CHD manifests at the start of their life and if left untreated, death occurs by the age of 20 years. The major genetic disorders in familial hypercholesterolemia and the frequency of their detection in the population are characterized. There are definitions of clinical screening options for FH: targeted, opportunistic, universal, cascadic. A comprehensive view of the diagnosis of FH according to the Dutch Lipid Clinic Network (DLCN) is provided. The basic principles of non-medication and three-step medication treatment of FH are presented. The article presents a clinical case of the homozygous FH taking into account the peculiarities of the disease course, the results of laboratory and instrumental studies and step-by-step treatment in the department of dyslipidemia of M.D. Strazhesko Institute of Cardiology of NAMS of Ukraine. The epidemiological data of the Ukrainian population survey on the possible prevalence of FH in Ukraine are presented. The preliminary analysis of the Ukrainian registry of patients with FH as a national fragment of the international ScreenProFH Registry and the European Register EAS-FHSC is provided.

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